This is a Validated Antibody Database (VAD) review about rat Sirt1, based on 39 published articles (read how Labome selects the articles), using Sirt1 antibody in all methods. It is aimed to help Labome visitors find the most suited Sirt1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Sirt1 synonym: Sir2

Knockout validation
Cell Signaling Technology
mouse monoclonal (1F3)
  • western blot knockout validation; human; fig 1
Cell Signaling Technology Sirt1 antibody (Cell Signaling, 8469) was used in western blot knockout validation on human samples (fig 1). Oncotarget (2016) ncbi
Cell Signaling Technology
mouse monoclonal (1F3)
  • western blot; human; fig 3
  • western blot knockout validation; mouse; fig 3
Cell Signaling Technology Sirt1 antibody (Santa Cruz, 1F3) was used in western blot on human samples (fig 3) and in western blot knockout validation on mouse samples (fig 3). Mol Cancer Ther (2015) ncbi
Abcam
mouse monoclonal (19A7AB4)
  • immunohistochemistry - paraffin section; rat; 1:100; loading ...; fig 9
Abcam Sirt1 antibody (Abcam, ab110304) was used in immunohistochemistry - paraffin section on rat samples at 1:100 (fig 9). Biomed Res Int (2022) ncbi
mouse monoclonal (19A7AB4)
  • western blot; mouse; 1:1000; loading ...; fig 8b
Abcam Sirt1 antibody (Abcam, ab110304) was used in western blot on mouse samples at 1:1000 (fig 8b). Mol Med Rep (2022) ncbi
mouse monoclonal (19A7AB4)
  • immunohistochemistry; human; loading ...; fig 7a
  • western blot; human; loading ...; fig 7c
Abcam Sirt1 antibody (Abcam, ab110304) was used in immunohistochemistry on human samples (fig 7a) and in western blot on human samples (fig 7c). Cell Death Dis (2021) ncbi
mouse monoclonal (19A7AB4)
  • immunohistochemistry; human; 1:30; loading ...; fig 1d
  • western blot; human; 1:2000; loading ...; fig 1b
Abcam Sirt1 antibody (Abcam, ab110304) was used in immunohistochemistry on human samples at 1:30 (fig 1d) and in western blot on human samples at 1:2000 (fig 1b). Oncol Rep (2021) ncbi
mouse monoclonal (19A7AB4)
  • western blot; mouse; loading ...; fig 2a
Abcam Sirt1 antibody (Abeam, abl 10304) was used in western blot on mouse samples (fig 2a). Cell (2021) ncbi
mouse monoclonal (19A7AB4)
  • other; rat; loading ...; fig 3h
  • western blot; rat; loading ...; fig 3c
Abcam Sirt1 antibody (Abcam, ab110304) was used in other on rat samples (fig 3h) and in western blot on rat samples (fig 3c). Aging (Albany NY) (2020) ncbi
mouse monoclonal (19A7AB4)
  • western blot; mouse; 1:500; fig 5c
Abcam Sirt1 antibody (Abcam, ab110304) was used in western blot on mouse samples at 1:500 (fig 5c). elife (2020) ncbi
mouse monoclonal (19A7AB4)
  • immunohistochemistry - frozen section; rat; 1:200; loading ...; fig 2e
Abcam Sirt1 antibody (Abcam, ab157401) was used in immunohistochemistry - frozen section on rat samples at 1:200 (fig 2e). Neurooncol Adv (2020) ncbi
mouse monoclonal (19A7AB4)
  • western blot; mouse; loading ...; fig 1e, 4a
Abcam Sirt1 antibody (Abcam, ab110304) was used in western blot on mouse samples (fig 1e, 4a). Front Physiol (2020) ncbi
mouse monoclonal (19A7AB4)
  • western blot; human; loading ...; fig 2e
Abcam Sirt1 antibody (Abcam, ab110304) was used in western blot on human samples (fig 2e). EBioMedicine (2020) ncbi
mouse monoclonal (19A7AB4)
  • immunohistochemistry; mouse; loading ...; fig 4b
  • western blot; mouse; loading ...; fig 4a
  • immunocytochemistry; human; loading ...; fig 1b
  • western blot; human; loading ...; fig 1a
Abcam Sirt1 antibody (Abcam, 110304) was used in immunohistochemistry on mouse samples (fig 4b), in western blot on mouse samples (fig 4a), in immunocytochemistry on human samples (fig 1b) and in western blot on human samples (fig 1a). Int J Chron Obstruct Pulmon Dis (2019) ncbi
mouse monoclonal (19A7AB4)
  • western blot; rat; loading ...; fig 6a
Abcam Sirt1 antibody (Abcam, ab110304) was used in western blot on rat samples (fig 6a). Braz J Med Biol Res (2019) ncbi
mouse monoclonal (19A7AB4)
  • western blot; mouse; 1:1000; fig 4
Abcam Sirt1 antibody (Abcam, ab110304) was used in western blot on mouse samples at 1:1000 (fig 4). Nat Commun (2015) ncbi
mouse monoclonal (19A7AB4)
  • immunohistochemistry - paraffin section; human; 1:50
In order to determine the role of Sirt1 in regulating the expression of tissue-restricted antigens in medullary thymic epithelial cells, Abcam Sirt1 antibody (Abcam, 110304) was used in immunohistochemistry - paraffin section on human samples at 1:50. Nat Immunol (2015) ncbi
mouse monoclonal (19A7AB4)
  • immunohistochemistry - paraffin section; mouse; 1:200
  • western blot; mouse; 1:1500
In order to investigate the effect of resveratrol on osteoarthritis in mice, Abcam Sirt1 antibody (Abcam, ab110304) was used in immunohistochemistry - paraffin section on mouse samples at 1:200 and in western blot on mouse samples at 1:1500. J Orthop Res (2015) ncbi
mouse monoclonal (19A7AB4)
  • western blot; rat; 1:2000; loading ...; fig 3a
Abcam Sirt1 antibody (Abcam, ab110304) was used in western blot on rat samples at 1:2000 (fig 3a). PLoS ONE (2014) ncbi
Santa Cruz Biotechnology
mouse monoclonal (B-7)
  • immunohistochemistry; mouse; 1:1000; loading ...; fig 6h
  • western blot; mouse; loading ...; fig 6f
Santa Cruz Biotechnology Sirt1 antibody (Santa Cruz, sc-74465) was used in immunohistochemistry on mouse samples at 1:1000 (fig 6h) and in western blot on mouse samples (fig 6f). Aging (Albany NY) (2021) ncbi
mouse monoclonal (B-7)
  • immunohistochemistry; rat; 1:200; loading ...; fig 9
Santa Cruz Biotechnology Sirt1 antibody (Santa Cruz, sc-74465) was used in immunohistochemistry on rat samples at 1:200 (fig 9). Biomolecules (2020) ncbi
mouse monoclonal (B-7)
  • western blot; rat; loading ...; fig 4c
Santa Cruz Biotechnology Sirt1 antibody (Santa Cruz, sc-74465) was used in western blot on rat samples (fig 4c). Biosci Rep (2019) ncbi
mouse monoclonal (B-7)
  • western blot; human; 1:500; fig 1
Santa Cruz Biotechnology Sirt1 antibody (Santa Cruz, sc-74465) was used in western blot on human samples at 1:500 (fig 1). Oncol Lett (2016) ncbi
mouse monoclonal (B-7)
  • western blot; rat; fig 5
Santa Cruz Biotechnology Sirt1 antibody (Santa Cruz, sc-74465) was used in western blot on rat samples (fig 5). Int J Med Sci (2016) ncbi
mouse monoclonal (B-7)
  • western blot; human; fig 2
Santa Cruz Biotechnology Sirt1 antibody (Santa Cruz, sc-74465) was used in western blot on human samples (fig 2). Sci Rep (2015) ncbi
mouse monoclonal (B-7)
  • western blot; human; loading ...; fig 5a
Santa Cruz Biotechnology Sirt1 antibody (Santa Cruz, sc-74465) was used in western blot on human samples (fig 5a). J Biol Chem (2015) ncbi
Novus Biologicals
mouse monoclonal (1F3)
  • immunohistochemistry; mouse; 1:1000; fig 4
Novus Biologicals Sirt1 antibody (Novus, NBP1-51641) was used in immunohistochemistry on mouse samples at 1:1000 (fig 4). Transl Psychiatry (2015) ncbi
Cell Signaling Technology
mouse monoclonal (1F3)
  • western blot; mouse; loading ...; fig 4b
Cell Signaling Technology Sirt1 antibody (Cell Signaling, 8469) was used in western blot on mouse samples (fig 4b). Front Physiol (2021) ncbi
mouse monoclonal (1F3)
  • western blot; mouse; 1:500; fig 5h
Cell Signaling Technology Sirt1 antibody (Cell Signaling, 8469) was used in western blot on mouse samples at 1:500 (fig 5h). BMC Biol (2021) ncbi
mouse monoclonal (1F3)
  • immunocytochemistry; human; loading ...
  • western blot; human; loading ...; fig 1a, 1c
Cell Signaling Technology Sirt1 antibody (Cell Signaling Technology, 8469) was used in immunocytochemistry on human samples and in western blot on human samples (fig 1a, 1c). elife (2020) ncbi
mouse monoclonal (1F3)
  • western blot; rat; 1:1000; loading ...; fig 4a
Cell Signaling Technology Sirt1 antibody (Cell Signaling, 8469S) was used in western blot on rat samples at 1:1000 (fig 4a). J Clin Invest (2020) ncbi
mouse monoclonal (1F3)
  • western blot; human; 1:1000; loading ...; fig 3c
Cell Signaling Technology Sirt1 antibody (Cell signaling, 1F3) was used in western blot on human samples at 1:1000 (fig 3c). Aging (Albany NY) (2019) ncbi
mouse monoclonal (1F3)
  • western blot; mouse; 1:1000; loading ...; fig 6e
Cell Signaling Technology Sirt1 antibody (Cell Signaling, 8469) was used in western blot on mouse samples at 1:1000 (fig 6e). Nat Commun (2018) ncbi
mouse monoclonal (1F3)
  • western blot; human; loading ...; fig 3a
In order to determine that SIRT1 is functionally required for sustaining the proliferation and survival of primary effusion lymphoma cells, Cell Signaling Technology Sirt1 antibody (Cell Signaling Technology, 8469) was used in western blot on human samples (fig 3a). J Pathol (2017) ncbi
mouse monoclonal (1F3)
  • western blot; human; loading ...; fig 7a
Cell Signaling Technology Sirt1 antibody (CST, 8469) was used in western blot on human samples (fig 7a). Cell Death Dis (2017) ncbi
mouse monoclonal (1F3)
  • western blot; mouse; loading ...; fig 4
In order to evaluate the antifibrotic effect of emodin in silica inhalation-induced lung fibrosis, Cell Signaling Technology Sirt1 antibody (Cell signaling, 8469) was used in western blot on mouse samples (fig 4). Mol Med Rep (2016) ncbi
mouse monoclonal (1F3)
  • western blot knockout validation; human; fig 1
Cell Signaling Technology Sirt1 antibody (Cell Signaling, 8469) was used in western blot knockout validation on human samples (fig 1). Oncotarget (2016) ncbi
mouse monoclonal (1F3)
  • western blot; human; 1:1000; fig 1
Cell Signaling Technology Sirt1 antibody (Cell Signaling, 8469) was used in western blot on human samples at 1:1000 (fig 1). PLoS ONE (2016) ncbi
mouse monoclonal (1F3)
  • western blot; human; 1:500; loading ...; fig 2
Cell Signaling Technology Sirt1 antibody (Cell signaling, 8469S) was used in western blot on human samples at 1:500 (fig 2). Oncotarget (2016) ncbi
mouse monoclonal (1F3)
  • western blot; rat; loading ...; fig 3a
In order to investigate the role of autophagy in the anti-neurotoxicity effect of resveratrol, Cell Signaling Technology Sirt1 antibody (Cell signaling, 8469S) was used in western blot on rat samples (fig 3a). Neurochem Res (2016) ncbi
mouse monoclonal (1F3)
  • western blot; human; fig 3
  • western blot knockout validation; mouse; fig 3
Cell Signaling Technology Sirt1 antibody (Santa Cruz, 1F3) was used in western blot on human samples (fig 3) and in western blot knockout validation on mouse samples (fig 3). Mol Cancer Ther (2015) ncbi
mouse monoclonal (1F3)
  • immunocytochemistry; hamsters; 1:100
  • western blot; hamsters
Cell Signaling Technology Sirt1 antibody (CST, 8469s) was used in immunocytochemistry on hamsters samples at 1:100 and in western blot on hamsters samples . J Mol Neurosci (2015) ncbi
Articles Reviewed
  1. Zhou Q, Li J, Xiang Z, Zou H, Shao X. Amelioration of Renal Injury by Resveratrol in a Rat Renal Transplantation Model via Activation of the SIRT1/NF-κB Signaling Pathway. Biomed Res Int. 2022;2022:7140961 pubmed publisher
  2. Yeh C, Liu H, Lee M, Leu Y, Chiang W, Chang H, et al. Phytochemical‑rich herbal formula ATG‑125 protects against sucrose‑induced gastrocnemius muscle atrophy by rescuing Akt signaling and improving mitochondrial dysfunction in young adult mice. Mol Med Rep. 2022;25: pubmed publisher
  3. Lü Z, Liu H, Song N, Liang Y, Zhu J, Chen J, et al. METTL14 aggravates podocyte injury and glomerulopathy progression through N6-methyladenosine-dependent downregulating of Sirt1. Cell Death Dis. 2021;12:881 pubmed publisher
  4. Wang X, Lu Y, Tuo Z, Zhou H, Zhang Y, Cao Z, et al. Role of SIRT1/AMPK signaling in the proliferation, migration and invasion of renal cell carcinoma cells. Oncol Rep. 2021;45: pubmed publisher
  5. Dong W, Zhang H, Zhao C, Luo Y, Chen Y. Silencing of miR-150-5p Ameliorates Diabetic Nephropathy by Targeting SIRT1/p53/AMPK Pathway. Front Physiol. 2021;12:624989 pubmed publisher
  6. Shin M, Vázquez Rosa E, Koh Y, Dhar M, Chaubey K, Cintrón Pérez C, et al. Reducing acetylated tau is neuroprotective in brain injury. Cell. 2021;184:2715-2732.e23 pubmed publisher
  7. Affortit C, Casas F, Ladrech S, Ceccato J, Bourien J, Coyat C, et al. Exacerbated age-related hearing loss in mice lacking the p43 mitochondrial T3 receptor. BMC Biol. 2021;19:18 pubmed publisher
  8. Huang S, You S, Qian J, Dai C, Shen S, Wang J, et al. Myeloid differentiation 2 deficiency attenuates AngII-induced arterial vascular oxidative stress, inflammation, and remodeling. Aging (Albany NY). 2021;13:4409-4427 pubmed publisher
  9. Meng F, Qian M, Peng B, Peng L, Wang X, Zheng K, et al. Synergy between SIRT1 and SIRT6 helps recognize DNA breaks and potentiates the DNA damage response and repair in humans and mice. elife. 2020;9: pubmed publisher
  10. Qian W, Cai X, Qian Q. Sirt1 antisense long non-coding RNA attenuates pulmonary fibrosis through sirt1-mediated epithelial-mesenchymal transition. Aging (Albany NY). 2020;12:4322-4336 pubmed publisher
  11. Zhang M, Du W, Acklin S, Jin S, Xia F. SIRT2 protects peripheral neurons from cisplatin-induced injury by enhancing nucleotide excision repair. J Clin Invest. 2020;130:2953-2965 pubmed publisher
  12. Che H, Li J, Li Y, Ma C, Liu H, Qin J, et al. p16 deficiency attenuates intervertebral disc degeneration by adjusting oxidative stress and nucleus pulposus cell cycle. elife. 2020;9: pubmed publisher
  13. Merlo S, Luaces J, Spampinato S, Toro Urrego N, Caruso G, D Amico F, et al. SIRT1 Mediates Melatonin's Effects on Microglial Activation in Hypoxia: In Vitro and In Vivo Evidence. Biomolecules. 2020;10: pubmed publisher
  14. Laws M, Bonomi R, Gelovani D, Llaniguez J, Lu X, Mangner T, et al. Noninvasive quantification of SIRT1 expression-activity and pharmacologic inhibition in a rat model of intracerebral glioma using 2-[18F]BzAHA PET/CT/MRI. Neurooncol Adv. 2020;2:vdaa006 pubmed publisher
  15. Li X, Zhu Q, Zheng R, Yan J, Wei M, Fan Y, et al. Puerarin Attenuates Diabetic Nephropathy by Promoting Autophagy in Podocytes. Front Physiol. 2020;11:73 pubmed publisher
  16. Xiang Q, Kang L, Wang J, Liao Z, Song Y, Zhao K, et al. CircRNA-CIDN mitigated compression loading-induced damage in human nucleus pulposus cells via miR-34a-5p/SIRT1 axis. EBioMedicine. 2020;53:102679 pubmed publisher
  17. Perri A, Agosti V, Olivo E, Concolino A, Angelis M, Tammè L, et al. Histone proteomics reveals novel post-translational modifications in breast cancer. Aging (Albany NY). 2019;11:11722-11755 pubmed publisher
  18. Wang J, Dong Z, Gui M, Yao L, Li J, Zhou X, et al. HuoXue QianYang QuTan Recipe attenuates left ventricular hypertrophy in obese hypertensive rats by improving mitochondrial function through SIRT1/PGC-1α deacetylation pathway. Biosci Rep. 2019;39: pubmed publisher
  19. Lai T, Wen X, Wu D, Su G, Gao Y, Chen C, et al. SIRT1 protects against urban particulate matter-induced airway inflammation. Int J Chron Obstruct Pulmon Dis. 2019;14:1741-1752 pubmed publisher
  20. Zhang X, Qin Q, Dai H, Cai S, Zhou C, Guan J. Emodin protects H9c2 cells from hypoxia-induced injury by up-regulating miR-138 expression. Braz J Med Biol Res. 2019;52:e7994 pubmed publisher
  21. Li T, Song L, Sun Y, Li J, Yi C, Lam S, et al. Tip60-mediated lipin 1 acetylation and ER translocation determine triacylglycerol synthesis rate. Nat Commun. 2018;9:1916 pubmed publisher
  22. He M, Tan B, Vasan K, Yuan H, Cheng F, Ramos da Silva S, et al. SIRT1 and AMPK pathways are essential for the proliferation and survival of primary effusion lymphoma cells. J Pathol. 2017;242:309-321 pubmed publisher
  23. Yuan H, Tan B, Gao S. Tenovin-6 impairs autophagy by inhibiting autophagic flux. Cell Death Dis. 2017;8:e2608 pubmed publisher
  24. Yang T, Wang J, Pang Y, Dang X, Ren H, Liu Y, et al. Emodin suppresses silica-induced lung fibrosis by promoting Sirt1 signaling via direct contact. Mol Med Rep. 2016;14:4643-4649 pubmed publisher
  25. He M, Yuan H, Tan B, Bai R, Kim H, Bae S, et al. SIRT1-mediated downregulation of p27Kip1 is essential for overcoming contact inhibition of Kaposi's sarcoma-associated herpesvirus transformed cells. Oncotarget. 2016;7:75698-75711 pubmed publisher
  26. Romeo S, Conti A, Polito F, Tomasello C, Barresi V, La Torre D, et al. miRNA regulation of Sirtuin-1 expression in human astrocytoma. Oncol Lett. 2016;12:2992-2998 pubmed
  27. Timucin A, Basaga H. SIRT6 Is a Positive Regulator of Aldose Reductase Expression in U937 and HeLa cells under Osmotic Stress: In Vitro and In Silico Insights. PLoS ONE. 2016;11:e0161494 pubmed publisher
  28. Wawrzyniak N, Joseph A, Levin D, Gundermann D, Leeuwenburgh C, Sandesara B, et al. Idiopathic chronic fatigue in older adults is linked to impaired mitochondrial content and biogenesis signaling in skeletal muscle. Oncotarget. 2016;7:52695-52709 pubmed publisher
  29. Deng H, Mi M. Resveratrol Attenuates A?25-35 Caused Neurotoxicity by Inducing Autophagy Through the TyrRS-PARP1-SIRT1 Signaling Pathway. Neurochem Res. 2016;41:2367-79 pubmed publisher
  30. Huang C, Wang T, Tung Y, Lin W. Effect of Exercise Training on Skeletal Muscle SIRT1 and PGC-1? Expression Levels in Rats of Different Age. Int J Med Sci. 2016;13:260-70 pubmed publisher
  31. Xiao X, Shi X, Fan Y, Zhang X, Wu M, Lan P, et al. GITR subverts Foxp3(+) Tregs to boost Th9 immunity through regulation of histone acetylation. Nat Commun. 2015;6:8266 pubmed publisher
  32. Lo Iacono L, Visco Comandini F, Valzania A, Viscomi M, Coviello M, Giampà A, et al. Adversity in childhood and depression: linked through SIRT1. Transl Psychiatry. 2015;5:e629 pubmed publisher
  33. Chuprin A, Avin A, Goldfarb Y, Herzig Y, Levi B, Jacob A, et al. The deacetylase Sirt1 is an essential regulator of Aire-mediated induction of central immunological tolerance. Nat Immunol. 2015;16:737-45 pubmed publisher
  34. Li W, Cai L, Zhang Y, Cui L, Shen G. Intra-articular resveratrol injection prevents osteoarthritis progression in a mouse model by activating SIRT1 and thereby silencing HIF-2α. J Orthop Res. 2015;33:1061-70 pubmed publisher
  35. Kobayashi K, Sakurai K, Hiramatsu H, Inada K, Shiogama K, Nakamura S, et al. The miR-199a/Brm/EGR1 axis is a determinant of anchorage-independent growth in epithelial tumor cell lines. Sci Rep. 2015;5:8428 pubmed publisher
  36. Peng L, Yuan Z, Li Y, Ling H, Izumi V, Fang B, et al. Ubiquitinated sirtuin 1 (SIRT1) function is modulated during DNA damage-induced cell death and survival. J Biol Chem. 2015;290:8904-12 pubmed publisher
  37. Torma F, Koltai E, Nagy E, Ziaaldini M, Posa A, Koch L, et al. Exercise Increases Markers of Spermatogenesis in Rats Selectively Bred for Low Running Capacity. PLoS ONE. 2014;9:e114075 pubmed publisher
  38. Lahusen T, Deng C. SRT1720 induces lysosomal-dependent cell death of breast cancer cells. Mol Cancer Ther. 2015;14:183-92 pubmed publisher
  39. Wang J, Zhang J, Shi Q, Zhang B, Chen C, Chen L, et al. Scrapie infection in experimental rodents and SMB-S15 cells decreased the brain endogenous levels and activities of Sirt1. J Mol Neurosci. 2015;55:1022-30 pubmed publisher