This is a Validated Antibody Database (VAD) review about rat Shh, based on 17 published articles (read how Labome selects the articles), using Shh antibody in all methods. It is aimed to help Labome visitors find the most suited Shh antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Shh synonym: ShhNC; sonic hedgehog protein; shh unprocessed N-terminal signaling and C-terminal autoprocessing domains; sonic hedgehog homolog

Knockout validation
Developmental Studies Hybridoma Bank
mouse monoclonal (5E1)
  • immunohistochemistry knockout validation; mouse; 1:20; loading ...; fig s8b
  • immunohistochemistry - paraffin section; mouse; 1:20; loading ...; fig 6b
Developmental Studies Hybridoma Bank Shh antibody (DSHB, 5E1) was used in immunohistochemistry knockout validation on mouse samples at 1:20 (fig s8b) and in immunohistochemistry - paraffin section on mouse samples at 1:20 (fig 6b). PLoS Genet (2017) ncbi
Developmental Studies Hybridoma Bank
mouse monoclonal (5E1)
  • immunohistochemistry knockout validation; common quail; loading ...; fig 6c
Developmental Studies Hybridoma Bank Shh antibody (DSHB, 5E1) was used in immunohistochemistry knockout validation on common quail samples (fig 6c). Front Cell Dev Biol (2016) ncbi
Abcam
rabbit polyclonal
  • western blot; rat; 1:500
Abcam Shh antibody (Abcam, ab19897) was used in western blot on rat samples at 1:500. Int Orthop (2013) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; human
Abcam Shh antibody (Abcam, ab19897) was used in immunohistochemistry - paraffin section on human samples . J Exp Clin Cancer Res (2013) ncbi
Cell Signaling Technology
rabbit monoclonal (C9C5)
  • western blot; human; loading ...; fig 5
Cell Signaling Technology Shh antibody (Cell Signaling, 2207) was used in western blot on human samples (fig 5). Mol Clin Oncol (2016) ncbi
rabbit monoclonal (C9C5)
  • western blot; mouse; fig 4
Cell Signaling Technology Shh antibody (Cell Signaling, 2207) was used in western blot on mouse samples (fig 4). Sci Rep (2016) ncbi
rabbit monoclonal (C9C5)
  • western blot; chicken; 1:600
Cell Signaling Technology Shh antibody (Cell Signaling, C9C5) was used in western blot on chicken samples at 1:600. Int J Mol Sci (2015) ncbi
rabbit monoclonal (C9C5)
  • immunohistochemistry - paraffin section; mouse; 1:100
  • western blot; mouse; 1:500
Cell Signaling Technology Shh antibody (Cell Signaling Technology, 2207) was used in immunohistochemistry - paraffin section on mouse samples at 1:100 and in western blot on mouse samples at 1:500. Mol Neurobiol (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 4
Cell Signaling Technology Shh antibody (Cell Signaling, 2271) was used in western blot on human samples (fig 4). Cancer Lett (2015) ncbi
rabbit monoclonal (C9C5)
  • western blot; mouse; 1:1000
Cell Signaling Technology Shh antibody (Cell Signaling, 2207) was used in western blot on mouse samples at 1:1000. Toxicol Appl Pharmacol (2014) ncbi
rabbit monoclonal (C9C5)
  • western blot; human
Cell Signaling Technology Shh antibody (Cell Signaling Technology, 2207) was used in western blot on human samples . PLoS ONE (2014) ncbi
rabbit monoclonal (C9C5)
  • western blot; human; loading ...; fig 8a
Cell Signaling Technology Shh antibody (Cell Signaling, 2207S) was used in western blot on human samples (fig 8a). Nature (2014) ncbi
rabbit monoclonal (C9C5)
  • immunohistochemistry - paraffin section; human
Cell Signaling Technology Shh antibody (Cell Signaling Technologies, 2207) was used in immunohistochemistry - paraffin section on human samples . Am J Pathol (2012) ncbi
Developmental Studies Hybridoma Bank
mouse monoclonal (5E1)
  • immunohistochemistry knockout validation; mouse; 1:20; loading ...; fig s8b
  • immunohistochemistry - paraffin section; mouse; 1:20; loading ...; fig 6b
Developmental Studies Hybridoma Bank Shh antibody (DSHB, 5E1) was used in immunohistochemistry knockout validation on mouse samples at 1:20 (fig s8b) and in immunohistochemistry - paraffin section on mouse samples at 1:20 (fig 6b). PLoS Genet (2017) ncbi
mouse monoclonal (5E1)
  • immunohistochemistry knockout validation; common quail; loading ...; fig 6c
Developmental Studies Hybridoma Bank Shh antibody (DSHB, 5E1) was used in immunohistochemistry knockout validation on common quail samples (fig 6c). Front Cell Dev Biol (2016) ncbi
mouse monoclonal (5E1)
  • immunohistochemistry; chicken; 1:200; fig s2
In order to determine regulation of neural tube closure by modulating tight junctions by cell-cycle-dependent TGFbeta-BMP antagonism, Developmental Studies Hybridoma Bank Shh antibody (DSHB, 5E1) was used in immunohistochemistry on chicken samples at 1:200 (fig s2). J Cell Sci (2017) ncbi
mouse monoclonal (5E1)
  • western blot; human; 1:500; fig 4b
In order to study human carcinoma-associated mesenchymal stem cells and ovarian cancer chemotherapy through a BMP4/HH signaling loop, Developmental Studies Hybridoma Bank Shh antibody (DSHB, 5E1) was used in western blot on human samples at 1:500 (fig 4b). Oncotarget (2016) ncbi
mouse monoclonal (5E1)
  • immunocytochemistry; mouse; 1:10; fig 3
Developmental Studies Hybridoma Bank Shh antibody (DSHB, 5E1) was used in immunocytochemistry on mouse samples at 1:10 (fig 3). Development (2014) ncbi
mouse monoclonal (5E1)
  • immunohistochemistry - paraffin section; Gallotia galloti; 1:2
In order to study the histogenesis of the lizard visual system, Developmental Studies Hybridoma Bank Shh antibody (DSHB, 5E1) was used in immunohistochemistry - paraffin section on Gallotia galloti samples at 1:2. J Comp Neurol (2012) ncbi
Articles Reviewed
  1. He H, Huang M, Sun S, Wu Y, Lin X. Epithelial heparan sulfate regulates Sonic Hedgehog signaling in lung development. PLoS Genet. 2017;13:e1006992 pubmed publisher
  2. Matsubara Y, Nakano M, Kawamura K, Tsudzuki M, Funahashi J, Agata K, et al. Inactivation of Sonic Hedgehog Signaling and Polydactyly in Limbs of Hereditary Multiple Malformation, a Novel Type of Talpid Mutant. Front Cell Dev Biol. 2016;4:149 pubmed publisher
  3. Galoian K, Luo S, Qureshi A, Patel P, Price R, Morse A, et al. Effect of cytostatic proline rich polypeptide-1 on tumor suppressors of inflammation pathway signaling in chondrosarcoma. Mol Clin Oncol. 2016;5:618-624 pubmed
  4. Singh V, Singh M, Gorantla S, Poluektova L, Maggirwar S. Smoothened Agonist Reduces Human Immunodeficiency Virus Type-1-Induced Blood-Brain Barrier Breakdown in Humanized Mice. Sci Rep. 2016;6:26876 pubmed publisher
  5. Amarnath S, Agarwala S. Cell-cycle-dependent TGF?-BMP antagonism regulates neural tube closure by modulating tight junctions. J Cell Sci. 2017;130:119-131 pubmed publisher
  6. Coffman L, Choi Y, McLean K, Allen B, di Magliano M, Buckanovich R. Human carcinoma-associated mesenchymal stem cells promote ovarian cancer chemotherapy resistance via a BMP4/HH signaling loop. Oncotarget. 2016;7:6916-32 pubmed publisher
  7. Juhász T, Szentléleky E, Somogyi C, Takács R, Dobrosi N, Engler M, et al. Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) Pathway Is Induced by Mechanical Load and Reduces the Activity of Hedgehog Signaling in Chondrogenic Micromass Cell Cultures. Int J Mol Sci. 2015;16:17344-67 pubmed publisher
  8. Hung Y, Chang S, Huang C, Yin J, Hwang C, Yang L, et al. Inhibitor of Differentiation-1 and Hypoxia-Inducible Factor-1 Mediate Sonic Hedgehog Induction by Amyloid Beta-Peptide in Rat Cortical Neurons. Mol Neurobiol. 2016;53:793-809 pubmed publisher
  9. Chen Y, Wei M, Wang C, Lee H, Pan S, Gao M, et al. Dual phosphoinositide 3-kinase/mammalian target of rapamycin inhibitor is an effective radiosensitizer for colorectal cancer. Cancer Lett. 2015;357:582-90 pubmed publisher
  10. Liu J, Bain L. Arsenic inhibits hedgehog signaling during P19 cell differentiation. Toxicol Appl Pharmacol. 2014;281:243-53 pubmed publisher
  11. So J, Lin J, Wahler J, Liby K, Sporn M, Suh N. A synthetic triterpenoid CDDO-Im inhibits tumorsphere formation by regulating stem cell signaling pathways in triple-negative breast cancer. PLoS ONE. 2014;9:e107616 pubmed publisher
  12. Alfaro A, Roberts B, Kwong L, Bijlsma M, Roelink H. Ptch2 mediates the Shh response in Ptch1-/- cells. Development. 2014;141:3331-9 pubmed publisher
  13. Zahreddine H, Culjkovic Kraljacic B, Assouline S, Gendron P, Romeo A, Morris S, et al. The sonic hedgehog factor GLI1 imparts drug resistance through inducible glucuronidation. Nature. 2014;511:90-3 pubmed publisher
  14. Ma X, Zhang X, Jia Y, Zu S, Han S, Xiao D, et al. Dexamethasone induces osteogenesis via regulation of hedgehog signalling molecules in rat mesenchymal stem cells. Int Orthop. 2013;37:1399-404 pubmed publisher
  15. Zhang Y, He J, Zhang F, Li H, Yue D, Wang C, et al. SMO expression level correlates with overall survival in patients with malignant pleural mesothelioma. J Exp Clin Cancer Res. 2013;32:7 pubmed publisher
  16. Cigna N, Farrokhi Moshai E, Brayer S, Marchal Sommé J, Wemeau Stervinou L, Fabre A, et al. The hedgehog system machinery controls transforming growth factor-?-dependent myofibroblastic differentiation in humans: involvement in idiopathic pulmonary fibrosis. Am J Pathol. 2012;181:2126-37 pubmed publisher
  17. Romero Alemán M, Monzon Mayor M, Santos E, Lang D, Yanes C. Neuronal and glial differentiation during lizard (Gallotia galloti) visual system ontogeny. J Comp Neurol. 2012;520:2163-84 pubmed publisher