This is a Validated Antibody Database (VAD) review about rat Shank3, based on 6 published articles (read how Labome selects the articles), using Shank3 antibody in all methods. It is aimed to help Labome visitors find the most suited Shank3 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Shank3 synonym: Prosap2; SH3 and multiple ankyrin repeat domains protein 3; SH3/ankyrin domain gene 3; SPANK-2; Shank postsynaptic density protein 3a; proline-rich synapse-associated protein 2

Knockout validation
Neuromab
mouse monoclonal (N69/46)
  • western blot knockout validation; rat; 1:100; loading ...; fig s1b
Neuromab Shank3 antibody (NeuroMab, N69/46) was used in western blot knockout validation on rat samples at 1:100 (fig s1b). elife (2017) ncbi
Synaptic Systems
rabbit polyclonal (/)
  • western blot; rat; fig 3
Synaptic Systems Shank3 antibody (SYSY, 162302) was used in western blot on rat samples (fig 3). Front Cell Neurosci (2015) ncbi
Neuromab
mouse monoclonal (N367/62)
  • western blot; rat; 1:100; loading ...; fig 1b
Neuromab Shank3 antibody (NeuroMab, N367/62) was used in western blot on rat samples at 1:100 (fig 1b). elife (2017) ncbi
mouse monoclonal (N69/46)
  • western blot knockout validation; rat; 1:100; loading ...; fig s1b
Neuromab Shank3 antibody (NeuroMab, N69/46) was used in western blot knockout validation on rat samples at 1:100 (fig s1b). elife (2017) ncbi
mouse monoclonal (N367/62)
  • western blot; mouse; fig 7
In order to analyze selective partitioning into complexes and supercomplexes during synapse maturation by NMDA receptors, Neuromab Shank3 antibody (NeuroMab, 75-344) was used in western blot on mouse samples (fig 7). Nat Commun (2016) ncbi
mouse monoclonal (N69/46)
  • western blot; rat; 1:10
In order to characterize rat cerebellar, hippocampal and cortical postsynaptic densities, Neuromab Shank3 antibody (Neuromab, 75-109) was used in western blot on rat samples at 1:10. Neuroscience (2015) ncbi
mouse monoclonal (N69/46)
  • immunoprecipitation; mouse; fig 1
Neuromab Shank3 antibody (UC Davis/NIH NeuroMab Facility, 75-109) was used in immunoprecipitation on mouse samples (fig 1). Int J Mol Sci (2015) ncbi
mouse monoclonal (N69/46)
  • immunohistochemistry; mouse
Neuromab Shank3 antibody (Neuromab, 75-109) was used in immunohistochemistry on mouse samples . Hear Res (2015) ncbi
Articles Reviewed
  1. Harony Nicolas H, Kay M, Hoffmann J, Klein M, Bozdagi Gunal O, Riad M, et al. Oxytocin improves behavioral and electrophysiological deficits in a novel Shank3-deficient rat. elife. 2017;6: pubmed publisher
  2. Frank R, Komiyama N, Ryan T, Zhu F, O Dell T, Grant S. NMDA receptors are selectively partitioned into complexes and supercomplexes during synapse maturation. Nat Commun. 2016;7:11264 pubmed publisher
  3. Garcia Manteiga J, Bonfiglio S, Folladori L, Malosio M, Lazarevic D, Stupka E, et al. REST-Governed Gene Expression Profiling in a Neuronal Cell Model Reveals Novel Direct and Indirect Processes of Repression and Up-Regulation. Front Cell Neurosci. 2015;9:438 pubmed publisher
  4. Farley M, Swulius M, Waxham M. Electron tomographic structure and protein composition of isolated rat cerebellar, hippocampal and cortical postsynaptic densities. Neuroscience. 2015;304:286-301 pubmed publisher
  5. Benthani F, Tran P, Currey N, Ng I, Giry Laterriere M, Carey L, et al. Proteogenomic Analysis Identifies a Novel Human SHANK3 Isoform. Int J Mol Sci. 2015;16:11522-30 pubmed publisher
  6. Braude J, Vijayakumar S, Baumgarner K, Laurine R, Jones T, Jones S, et al. Deletion of Shank1 has minimal effects on the molecular composition and function of glutamatergic afferent postsynapses in the mouse inner ear. Hear Res. 2015;321:52-64 pubmed publisher