This is a Validated Antibody Database (VAD) review about rat Scn5a, based on 23 published articles (read how Labome selects the articles), using Scn5a antibody in all methods. It is aimed to help Labome visitors find the most suited Scn5a antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Scn5a synonym: Nav1.5; SCAL

Alomone Labs
domestic rabbit polyclonal
  • immunocytochemistry; rat; loading ...; fig 4c
  • western blot; rat; 1:200; loading ...; fig 4a
  • western blot; mouse; loading ...; fig 4a
Alomone Labs Scn5a antibody (Alomone Labs, ASC-005) was used in immunocytochemistry on rat samples (fig 4c), in western blot on rat samples at 1:200 (fig 4a) and in western blot on mouse samples (fig 4a). Cell Death Dis (2021) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - frozen section; rat; 1:50; loading ...; fig 5a
In order to suggest that ectopic beats originating in the myocardial sleeves of the pulmonary veins occur only under pathological conditions, Alomone Labs Scn5a antibody (Alomone Labs, ASC-005) was used in immunohistochemistry - frozen section on rat samples at 1:50 (fig 5a). Exp Ther Med (2016) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; mouse; 1:50; loading ...; fig 1b
  • western blot; mouse; 1:500; loading ...; fig 7d
Alomone Labs Scn5a antibody (Alomone Labs, ASC-005) was used in immunohistochemistry on mouse samples at 1:50 (fig 1b) and in western blot on mouse samples at 1:500 (fig 7d). J Clin Invest (2016) ncbi
MilliporeSigma
mouse monoclonal (K58/35)
  • immunohistochemistry; rat; 1:200; fig 8o
MilliporeSigma Scn5a antibody (Sigma-Aldrich, S8809) was used in immunohistochemistry on rat samples at 1:200 (fig 8o). Sci Adv (2022) ncbi
mouse monoclonal (K58/35)
  • immunocytochemistry; mouse; 1:100; fig 3c
MilliporeSigma Scn5a antibody (Sigma-Aldrich, S8809-.1MG) was used in immunocytochemistry on mouse samples at 1:100 (fig 3c). Development (2022) ncbi
mouse monoclonal (K58/35)
  • immunocytochemistry; human; fig 6h
MilliporeSigma Scn5a antibody (Sigma-Aldrich, S8809) was used in immunocytochemistry on human samples (fig 6h). Cell Rep Med (2021) ncbi
mouse monoclonal (K58/35)
  • immunohistochemistry; mouse; 1:400; loading ...; fig 5b
MilliporeSigma Scn5a antibody (Sigma?CAldrich, K58/35) was used in immunohistochemistry on mouse samples at 1:400 (fig 5b). Commun Biol (2020) ncbi
mouse monoclonal (K58/35)
  • western blot; human; 1:2000; loading ...; fig 2a
MilliporeSigma Scn5a antibody (Sigma Aldrich, S8809) was used in western blot on human samples at 1:2000 (fig 2a). elife (2020) ncbi
mouse monoclonal (K58/35)
  • immunocytochemistry; rat; 1:100; loading ...; fig 3a
MilliporeSigma Scn5a antibody (Sigma-Aldrich, S8809) was used in immunocytochemistry on rat samples at 1:100 (fig 3a). Cell Death Differ (2019) ncbi
mouse monoclonal (K58/35)
  • immunocytochemistry; rat; 1:1000; loading ...; fig 1a
MilliporeSigma Scn5a antibody (Sigma, S8809) was used in immunocytochemistry on rat samples at 1:1000 (fig 1a). Front Cell Neurosci (2019) ncbi
mouse monoclonal (K58/35)
  • western blot; rat; 1:200; loading ...; fig 8
In order to demonstrate that fibroblast growth factor 14 affects KCNQ channel function and localization, MilliporeSigma Scn5a antibody (Sigma, K58/35) was used in western blot on rat samples at 1:200 (fig 8). Proc Natl Acad Sci U S A (2017) ncbi
mouse monoclonal (K58/35)
  • immunohistochemistry - frozen section; mouse; 1:250; loading ...; fig 4d
In order to examine the role of Opalin in mammalian myelination, MilliporeSigma Scn5a antibody (Sigma, S8809) was used in immunohistochemistry - frozen section on mouse samples at 1:250 (fig 4d). PLoS ONE (2016) ncbi
mouse monoclonal (K58/35)
  • western blot; mouse; fig 1
MilliporeSigma Scn5a antibody (Sigma Aldrich, K58/35) was used in western blot on mouse samples (fig 1). Nat Commun (2016) ncbi
mouse monoclonal (K58/35)
  • immunohistochemistry - frozen section; rat; 1:100; loading ...; fig 2a
MilliporeSigma Scn5a antibody (Sigma-Aldrich, S8809) was used in immunohistochemistry - frozen section on rat samples at 1:100 (fig 2a). J Comp Neurol (2017) ncbi
mouse monoclonal (K58/35)
  • immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig 5a
  • immunoprecipitation; mouse; 1:200; loading ...; fig 4a
MilliporeSigma Scn5a antibody (Sigma-Aldrich, S8809) was used in immunohistochemistry - frozen section on mouse samples at 1:200 (fig 5a) and in immunoprecipitation on mouse samples at 1:200 (fig 4a). J Neurosci (2016) ncbi
mouse monoclonal (K58/35)
  • immunohistochemistry - frozen section; mouse; 1:300; fig s7
MilliporeSigma Scn5a antibody (Sigma, S8809) was used in immunohistochemistry - frozen section on mouse samples at 1:300 (fig s7). Front Cell Neurosci (2016) ncbi
mouse monoclonal (K58/35)
  • immunoprecipitation; mouse; fig 6
  • western blot; mouse; 1:1000; fig 1
In order to study native cerebellar iFGF14 complexes by proteomic analysis, MilliporeSigma Scn5a antibody (Sigma, S8809) was used in immunoprecipitation on mouse samples (fig 6) and in western blot on mouse samples at 1:1000 (fig 1). Channels (Austin) (2016) ncbi
mouse monoclonal (K58/35)
  • western blot; guinea pig; 1:800; fig 6
MilliporeSigma Scn5a antibody (Sigma-Aldrich, S8809) was used in western blot on guinea pig samples at 1:800 (fig 6). PLoS ONE (2016) ncbi
mouse monoclonal (K58/35)
  • western blot; mouse
In order to examine the effects of Kidins220 ablation on neuronal excitability, MilliporeSigma Scn5a antibody (Sigma Aldrich, S8809) was used in western blot on mouse samples . J Biol Chem (2015) ncbi
mouse monoclonal (K58/35)
  • western blot; human; fig s6
MilliporeSigma Scn5a antibody (Sigma, S8809) was used in western blot on human samples (fig s6). PLoS ONE (2015) ncbi
mouse monoclonal (K58/35)
  • immunocytochemistry; rat; loading ...; fig 2e
MilliporeSigma Scn5a antibody (Sigma-Aldrich, S8809) was used in immunocytochemistry on rat samples (fig 2e). J Cell Biol (2015) ncbi
mouse monoclonal (K58/35)
  • immunocytochemistry; rat; 1:100; fig 6
MilliporeSigma Scn5a antibody (Sigma, S8809) was used in immunocytochemistry on rat samples at 1:100 (fig 6). J Neurosci (2015) ncbi
mouse monoclonal (K58/35)
  • immunohistochemistry; mouse; 1:100
In order to use multicolor labeling technology to simultaneously individual oligodendrocytes in the postnatal mouse optic nerve, MilliporeSigma Scn5a antibody (Sigma, S8809) was used in immunohistochemistry on mouse samples at 1:100. Glia (2015) ncbi
Articles Reviewed
  1. Dorrego Rivas A, Ezan J, Moreau M, Poirault Chassac S, Aubailly N, De Neve J, et al. The core PCP protein Prickle2 regulates axon number and AIS maturation by binding to AnkG and modulating microtubule bundling. Sci Adv. 2022;8:eabo6333 pubmed publisher
  2. Tian T, Quintana Urzainqui I, Kozi x107 Z, Pratt T, Price D. Pax6 loss alters the morphological and electrophysiological development of mouse prethalamic neurons. Development. 2022;149: pubmed publisher
  3. Clark A, Kugathasan U, Baskozos G, Priestman D, Fugger N, Lone M, et al. An iPSC model of hereditary sensory neuropathy-1 reveals L-serine-responsive deficits in neuronal ganglioside composition and axoglial interactions. Cell Rep Med. 2021;2:100345 pubmed publisher
  4. Liu X, Ge J, Chen C, Shen Y, Xie J, Zhu X, et al. FAT10 protects against ischemia-induced ventricular arrhythmia by decreasing Nedd4-2/Nav1.5 complex formation. Cell Death Dis. 2021;12:25 pubmed publisher
  5. Otani Y, Ohno N, Cui J, Yamaguchi Y, Baba H. Upregulation of large myelin protein zero leads to Charcot-Marie-Tooth disease-like neuropathy in mice. Commun Biol. 2020;3:121 pubmed publisher
  6. Yang H, Pérez Hernández M, Sanchez Alonso J, Shevchuk A, Gorelik J, Rothenberg E, et al. Ankyrin-G mediates targeting of both Na+ and KATP channels to the rat cardiac intercalated disc. elife. 2020;9: pubmed publisher
  7. Aprile D, Fruscione F, Baldassari S, Fadda M, Ferrante D, Falace A, et al. TBC1D24 regulates axonal outgrowth and membrane trafficking at the growth cone in rodent and human neurons. Cell Death Differ. 2019;: pubmed publisher
  8. Alpizar S, BAKER A, Gulledge A, Hoppa M. Loss of Neurofascin-186 Disrupts Alignment of AnkyrinG Relative to Its Binding Partners in the Axon Initial Segment. Front Cell Neurosci. 2019;13:1 pubmed publisher
  9. Pablo J, Pitt G. FGF14 is a regulator of KCNQ2/3 channels. Proc Natl Acad Sci U S A. 2017;114:154-159 pubmed publisher
  10. Xiao Y, Cai X, Atkinson A, Logantha S, Boyett M, Dobrzynski H. Expression of connexin 43, ion channels and Ca2+-handling proteins in rat pulmonary vein cardiomyocytes. Exp Ther Med. 2016;12:3233-3241 pubmed
  11. Yoshikawa F, Sato Y, Tohyama K, Akagi T, Furuse T, Sadakata T, et al. Mammalian-Specific Central Myelin Protein Opalin Is Redundant for Normal Myelination: Structural and Behavioral Assessments. PLoS ONE. 2016;11:e0166732 pubmed publisher
  12. Shekhar A, Lin X, Liu F, Zhang J, Mo H, Bastarache L, et al. Transcription factor ETV1 is essential for rapid conduction in the heart. J Clin Invest. 2016;126:4444-4459 pubmed publisher
  13. Dover K, Marra C, Solinas S, Popovic M, Subramaniyam S, Zecevic D, et al. FHF-independent conduction of action potentials along the leak-resistant cerebellar granule cell axon. Nat Commun. 2016;7:12895 pubmed publisher
  14. Serwanski D, Jukkola P, Nishiyama A. Heterogeneity of astrocyte and NG2 cell insertion at the node of ranvier. J Comp Neurol. 2017;525:535-552 pubmed publisher
  15. Kruger L, O Malley H, Hull J, Kleeman A, Patino G, Isom L. ?1-C121W Is Down But Not Out: Epilepsy-Associated Scn1b-C121W Results in a Deleterious Gain-of-Function. J Neurosci. 2016;36:6213-24 pubmed publisher
  16. Alshammari M, Alshammari T, Laezza F. Improved Methods for Fluorescence Microscopy Detection of Macromolecules at the Axon Initial Segment. Front Cell Neurosci. 2016;10:5 pubmed publisher
  17. Bosch M, Nerbonne J, Townsend R, Miyazaki H, Nukina N, Ornitz D, et al. Proteomic analysis of native cerebellar iFGF14 complexes. Channels (Austin). 2016;10:297-312 pubmed publisher
  18. Nassal D, Wan X, Liu H, Deschenes I. Myocardial KChIP2 Expression in Guinea Pig Resolves an Expanded Electrophysiologic Role. PLoS ONE. 2016;11:e0146561 pubmed publisher
  19. Cesca F, Satapathy A, Ferrea E, Nieus T, Benfenati F, Scholz Starke J. Functional Interaction between the Scaffold Protein Kidins220/ARMS and Neuronal Voltage-Gated Na+ Channels. J Biol Chem. 2015;290:18045-55 pubmed publisher
  20. Koenig J, Werdehausen R, Linley J, Habib A, Vernon J, Lolignier S, et al. Regulation of Nav1.7: A Conserved SCN9A Natural Antisense Transcript Expressed in Dorsal Root Ganglia. PLoS ONE. 2015;10:e0128830 pubmed publisher
  21. Colombelli C, Palmisano M, Eshed Eisenbach Y, Zambroni D, Pavoni E, Ferri C, et al. Perlecan is recruited by dystroglycan to nodes of Ranvier and binds the clustering molecule gliomedin. J Cell Biol. 2015;208:313-29 pubmed publisher
  22. Chand A, Galliano E, Chesters R, Grubb M. A distinct subtype of dopaminergic interneuron displays inverted structural plasticity at the axon initial segment. J Neurosci. 2015;35:1573-90 pubmed publisher
  23. Dumas L, Heitz Marchaland C, Fouquet S, Suter U, Livet J, Moreau Fauvarque C, et al. Multicolor analysis of oligodendrocyte morphology, interactions, and development with Brainbow. Glia. 2015;63:699-717 pubmed publisher