This is a Validated Antibody Database (VAD) review about rat SAP97, based on 23 published articles (read how Labome selects the articles), using SAP97 antibody in all methods. It is aimed to help Labome visitors find the most suited SAP97 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
SAP97 synonym: Dlgh1; SAP97

Knockout validation
Santa Cruz Biotechnology
mouse monoclonal (2D11)
  • western blot knockout validation; dogs; loading ...; fig s3
  • immunocytochemistry; dogs; loading ...; fig s3
Santa Cruz Biotechnology SAP97 antibody (Santa Cruz, sc-9961) was used in western blot knockout validation on dogs samples (fig s3) and in immunocytochemistry on dogs samples (fig s3). PLoS Pathog (2021) ncbi
Santa Cruz Biotechnology
mouse monoclonal (2D11)
  • western blot knockout validation; dogs; loading ...; fig s3
  • immunocytochemistry; dogs; loading ...; fig s3
Santa Cruz Biotechnology SAP97 antibody (Santa Cruz, sc-9961) was used in western blot knockout validation on dogs samples (fig s3) and in immunocytochemistry on dogs samples (fig s3). PLoS Pathog (2021) ncbi
mouse monoclonal (2D11)
  • immunohistochemistry; mouse; loading ...; fig 3c
In order to report that SB216763 prevents myocyte injury and cardiac dysfunction in two murine models of arrhythmogenic cardiomyopathy, Santa Cruz Biotechnology SAP97 antibody (Santa Cruz, sc-9961) was used in immunohistochemistry on mouse samples (fig 3c). JCI Insight (2016) ncbi
mouse monoclonal (2D11)
  • immunohistochemistry - paraffin section; human; 1:40; loading ...; fig 1a
  • western blot; human; 1:100; loading ...; fig 4a
In order to study the role of cell polarity determinant human Discs-large in endometrial cancer, Santa Cruz Biotechnology SAP97 antibody (Santa Cruz, sc-9961) was used in immunohistochemistry - paraffin section on human samples at 1:40 (fig 1a) and in western blot on human samples at 1:100 (fig 4a). Br J Cancer (2016) ncbi
mouse monoclonal (2D11)
  • western blot; human; fig 6
Santa Cruz Biotechnology SAP97 antibody (Santa Cruz, sc-9961) was used in western blot on human samples (fig 6). Int J Biochem Cell Biol (2015) ncbi
mouse monoclonal (2D11)
  • immunoprecipitation; human
Santa Cruz Biotechnology SAP97 antibody (Santa Cruz Biotechnology, sc-9961) was used in immunoprecipitation on human samples . J Biol Chem (2013) ncbi
Invitrogen
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...
Invitrogen SAP97 antibody (Thermo, PA1-741) was used in western blot on mouse samples at 1:1000. Cell Death Dis (2021) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; rat; 1:1000; loading ...; fig 3a
In order to investigate diverging presynaptic and postsynaptic roles of SAP97 N-terminal isoforms in synapse maturation and plasticity, Invitrogen SAP97 antibody (Thermo Scientific, PA1-741) was used in immunocytochemistry on rat samples at 1:1000 (fig 3a). Hippocampus (2017) ncbi
domestic rabbit polyclonal
  • western blot; mouse; fig 9
In order to analyze modulation by donepezil and diet on mTOR signaling and neuroinflammation in the brain by donepezil and diet, Invitrogen SAP97 antibody (Pierce, PA1-741) was used in western blot on mouse samples (fig 9). Biochim Biophys Acta (2016) ncbi
domestic rabbit polyclonal
In order to identify modulators of corticotropin-releasing hormone receptor type 1 signaling, Invitrogen SAP97 antibody (Thermo Fisher Scientific, PA1-741) was used . PLoS ONE (2015) ncbi
domestic rabbit polyclonal
In order to demonstrate that the dendrite branching promoting action of full length SAP97 is dependent on the PDZ3 domain, Invitrogen SAP97 antibody (ThermoFisher Scientific, PA1-741) was used . Mol Cell Neurosci (2015) ncbi
domestic rabbit polyclonal
In order to explore how the different domains of SAP97 regulate its localization, Invitrogen SAP97 antibody (Thermo, PA1-044) was used . Biochim Biophys Acta (2015) ncbi
Synaptic Systems
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 4a
Synaptic Systems SAP97 antibody (Synaptic Systems, 124302) was used in western blot on mouse samples at 1:1000 (fig 4a). Cell Rep (2019) ncbi
Neuromab
mouse monoclonal (K64/15)
  • immunohistochemistry; mouse; 1:250; loading ...; fig 1c
Neuromab SAP97 antibody (UC Davis/NIH Neuromab, 75-030) was used in immunohistochemistry on mouse samples at 1:250 (fig 1c). Front Cell Neurosci (2021) ncbi
mouse monoclonal (K64/15)
  • western blot; rat; 1:1000; loading ...; fig 1b
Neuromab SAP97 antibody (UC Davis/NIH NeuroMab Facil, 75-030) was used in western blot on rat samples at 1:1000 (fig 1b). Theranostics (2021) ncbi
mouse monoclonal (K64/15)
  • western blot; mouse; loading ...; fig s4b
Neuromab SAP97 antibody (NeuroMab, 75-030) was used in western blot on mouse samples (fig s4b). Proc Natl Acad Sci U S A (2021) ncbi
mouse monoclonal (K64/15)
  • western blot; mouse; 1:50; loading ...; fig 1d
  • western blot; human; 1:50; loading ...; fig 1d
Neuromab SAP97 antibody (UC Davis/NIH NeuroMab Facility and Antibodies, K64/15) was used in western blot on mouse samples at 1:50 (fig 1d) and in western blot on human samples at 1:50 (fig 1d). Sci Rep (2017) ncbi
mouse monoclonal (K64/15)
  • immunohistochemistry; domestic rabbit; 1:500; loading ...; tbl 1
In order to identify and investigate binding partners of SAP102, Neuromab SAP97 antibody (UC Davis/NIH NeuroMab Facility, 75-030) was used in immunohistochemistry on domestic rabbit samples at 1:500 (tbl 1). J Comp Neurol (2017) ncbi
mouse monoclonal (K64/15)
  • western blot; mouse; 1:1000; loading ...; fig 6e
Neuromab SAP97 antibody (Neuromab, 75-030) was used in western blot on mouse samples at 1:1000 (fig 6e). J Neurosci (2016) ncbi
mouse monoclonal (K64/15)
  • western blot; mouse; fig 7
In order to analyze selective partitioning into complexes and supercomplexes during synapse maturation by NMDA receptors, Neuromab SAP97 antibody (NeuroMab, 75-030) was used in western blot on mouse samples (fig 7). Nat Commun (2016) ncbi
mouse monoclonal (K64/15)
  • western blot; mouse; fig 8
Neuromab SAP97 antibody (Antibodies Incorporated, 75-030) was used in western blot on mouse samples (fig 8). Proc Natl Acad Sci U S A (2016) ncbi
mouse monoclonal (K64/15)
  • western blot; human
In order to demonstrate that the dendrite branching promoting action of full length SAP97 is dependent on the PDZ3 domain, Neuromab SAP97 antibody (UC Davis/NIH NeuroMab Facility, 75-030) was used in western blot on human samples . Mol Cell Neurosci (2015) ncbi
mouse monoclonal (K64/15)
  • western blot; rat; 1:1000
In order to study the roles of the NR2A and NR2B subunits, and of their scaffolding proteins, in remodeling the dendritic architecture of developing hippocampal neurons, Neuromab SAP97 antibody (UC Davis/NIH NeuroMab Facility, 75-030) was used in western blot on rat samples at 1:1000. PLoS ONE (2014) ncbi
mouse monoclonal (K64/15)
  • western blot; mouse; 1:1000
Neuromab SAP97 antibody (NeuroMab, K64/15) was used in western blot on mouse samples at 1:1000. J Neurosci (2013) ncbi
mouse monoclonal (K64/15)
  • western blot; rat
In order to examine the roles of SAP102 in cortical synapse development, Neuromab SAP97 antibody (NeuroMab, 75-030) was used in western blot on rat samples . J Neurosci (2013) ncbi
Articles Reviewed
  1. Vila A, Shihabeddin E, Zhang Z, Santhanam A, Ribelayga C, O BRIEN J. Synaptic Scaffolds, Ion Channels and Polyamines in Mouse Photoreceptor Synapses: Anatomy of a Signaling Complex. Front Cell Neurosci. 2021;15:667046 pubmed publisher
  2. Bourgeois J, Wang L, Rabino A, Everitt J, Alvarez M, Awadia S, et al. ARHGEF26 enhances Salmonella invasion and inflammation in cells and mice. PLoS Pathog. 2021;17:e1009713 pubmed publisher
  3. Ayuso Dolado S, Esteban Ortega G, Vidaurre O, Díaz Guerra M. A novel cell-penetrating peptide targeting calpain-cleavage of PSD-95 induced by excitotoxicity improves neurological outcome after stroke. Theranostics. 2021;11:6746-6765 pubmed publisher
  4. Liu X, Ge J, Chen C, Shen Y, Xie J, Zhu X, et al. FAT10 protects against ischemia-induced ventricular arrhythmia by decreasing Nedd4-2/Nav1.5 complex formation. Cell Death Dis. 2021;12:25 pubmed publisher
  5. Fukata Y, Chen X, Chiken S, Hirano Y, Yamagata A, Inahashi H, et al. LGI1-ADAM22-MAGUK configures transsynaptic nanoalignment for synaptic transmission and epilepsy prevention. Proc Natl Acad Sci U S A. 2021;118: pubmed publisher
  6. Wagner W, Lippmann K, Heisler F, Gromova K, Lombino F, Roesler M, et al. Myosin VI Drives Clathrin-Mediated AMPA Receptor Endocytosis to Facilitate Cerebellar Long-Term Depression. Cell Rep. 2019;28:11-20.e9 pubmed publisher
  7. Fragkouli A, Koukouraki P, Vlachos I, Paraskevopoulou M, Hatzigeorgiou A, Doxakis E. Neuronal ELAVL proteins utilize AUF-1 as a co-partner to induce neuron-specific alternative splicing of APP. Sci Rep. 2017;7:44507 pubmed publisher
  8. Goodman L, Baddeley D, Ambroziak W, Waites C, Garner C, Soeller C, et al. N-terminal SAP97 isoforms differentially regulate synaptic structure and postsynaptic surface pools of AMPA receptors. Hippocampus. 2017;27:668-682 pubmed publisher
  9. Vila A, Whitaker C, O BRIEN J. Membrane-associated guanylate kinase scaffolds organize a horizontal cell synaptic complex restricted to invaginating contacts with photoreceptors. J Comp Neurol. 2017;525:850-867 pubmed publisher
  10. Sim S, Lim C, Kim J, Seo D, Chun H, Yu N, et al. The Brain-Enriched MicroRNA miR-9-3p Regulates Synaptic Plasticity and Memory. J Neurosci. 2016;36:8641-52 pubmed publisher
  11. Chelko S, Asimaki A, Andersen P, Bedja D, Amat Alarcon N, Demazumder D, et al. Central role for GSK3β in the pathogenesis of arrhythmogenic cardiomyopathy. JCI Insight. 2016;1: pubmed publisher
  12. Frank R, Komiyama N, Ryan T, Zhu F, O Dell T, Grant S. NMDA receptors are selectively partitioned into complexes and supercomplexes during synapse maturation. Nat Commun. 2016;7:11264 pubmed publisher
  13. Sugihara T, Nakagawa S, Sasajima Y, Ichinose T, Hiraike H, Kondo F, et al. Loss of the cell polarity determinant human Discs-large is a novel molecular marker of nodal involvement and poor prognosis in endometrial cancer. Br J Cancer. 2016;114:1012-8 pubmed publisher
  14. Li W, Xu X, Pozzo Miller L. Excitatory synapses are stronger in the hippocampus of Rett syndrome mice due to altered synaptic trafficking of AMPA-type glutamate receptors. Proc Natl Acad Sci U S A. 2016;113:E1575-84 pubmed publisher
  15. Dasuri K, Zhang L, Kim S, Bruce Keller A, Keller J. Dietary and donepezil modulation of mTOR signaling and neuroinflammation in the brain. Biochim Biophys Acta. 2016;1862:274-83 pubmed publisher
  16. Bender J, Engeholm M, Ederer M, Breu J, Møller T, Michalakis S, et al. Corticotropin-Releasing Hormone Receptor Type 1 (CRHR1) Clustering with MAGUKs Is Mediated via Its C-Terminal PDZ Binding Motif. PLoS ONE. 2015;10:e0136768 pubmed publisher
  17. Zhang L, Hsu F, Mojsilovic Petrovic J, Jablonski A, Zhai J, Coulter D, et al. Structure-function analysis of SAP97, a modular scaffolding protein that drives dendrite growth. Mol Cell Neurosci. 2015;65:31-44 pubmed publisher
  18. Menga A, Iacobazzi V, Infantino V, Avantaggiati M, Palmieri F. The mitochondrial aspartate/glutamate carrier isoform 1 gene expression is regulated by CREB in neuronal cells. Int J Biochem Cell Biol. 2015;60:157-66 pubmed publisher
  19. Hong X, Avetisyan M, Ronilo M, Standley S. SAP97 blocks the RXR ER retention signal of NMDA receptor subunit GluN1-3 through its SH3 domain. Biochim Biophys Acta. 2015;1853:489-99 pubmed publisher
  20. Bustos F, Varela Nallar L, Campos M, Henriquez B, Phillips M, Opazo C, et al. PSD95 suppresses dendritic arbor development in mature hippocampal neurons by occluding the clustering of NR2B-NMDA receptors. PLoS ONE. 2014;9:e94037 pubmed publisher
  21. Hoy J, Haeger P, Constable J, Arias R, McCallum R, Kyweriga M, et al. Neuroligin1 drives synaptic and behavioral maturation through intracellular interactions. J Neurosci. 2013;33:9364-84 pubmed publisher
  22. Philippe M, Léger T, Desvaux R, Walch L. Discs large 1 (Dlg1) scaffolding protein participates with clathrin and adaptator protein complex 1 (AP-1) in forming Weibel-Palade bodies of endothelial cells. J Biol Chem. 2013;288:13046-56 pubmed publisher
  23. Murata Y, Constantine Paton M. Postsynaptic density scaffold SAP102 regulates cortical synapse development through EphB and PAK signaling pathway. J Neurosci. 2013;33:5040-52 pubmed publisher