This is a Validated Antibody Database (VAD) review about rat Runx1, based on 14 published articles (read how Labome selects the articles), using Runx1 antibody in all methods. It is aimed to help Labome visitors find the most suited Runx1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Runx1 synonym: Aml1; CBF-alpha-2; Cbfa2; PEA2-alpha; PEBP2-alpha

Knockout validation
Abcam
domestic rabbit polyclonal
  • immunohistochemistry knockout validation; mouse; loading ...; fig 1c
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 2a
Abcam Runx1 antibody (Abcam, 23980) was used in immunohistochemistry knockout validation on mouse samples (fig 1c) and in immunohistochemistry - paraffin section on mouse samples (fig 2a). Sci Rep (2019) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D4A6)
  • western blot knockout validation; mouse; loading ...; fig 3c, 7d
Cell Signaling Technology Runx1 antibody (Cell signaling, 8529s) was used in western blot knockout validation on mouse samples (fig 3c, 7d). PLoS Genet (2021) ncbi
Abcam
domestic rabbit polyclonal
  • ChIP-Seq; human; ; loading ...; fig 2c
  • immunohistochemistry - paraffin section; human; 1:100; loading ...; fig 6c
Abcam Runx1 antibody (Abcam, ab23980) was used in ChIP-Seq on human samples at (fig 2c) and in immunohistochemistry - paraffin section on human samples at 1:100 (fig 6c). PLoS Genet (2021) ncbi
domestic rabbit polyclonal
  • immunohistochemistry knockout validation; mouse; loading ...; fig 1c
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 2a
Abcam Runx1 antibody (Abcam, 23980) was used in immunohistochemistry knockout validation on mouse samples (fig 1c) and in immunohistochemistry - paraffin section on mouse samples (fig 2a). Sci Rep (2019) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:100; loading ...; fig 7f
  • chromatin immunoprecipitation; human; loading ...; fig 7d
  • immunocytochemistry; human; 1:100; loading ...; fig 1g
Abcam Runx1 antibody (Abcam, ab23980) was used in immunohistochemistry - paraffin section on human samples at 1:100 (fig 7f), in chromatin immunoprecipitation on human samples (fig 7d) and in immunocytochemistry on human samples at 1:100 (fig 1g). Nat Commun (2019) ncbi
domestic rabbit polyclonal
  • ChIP-Seq; human; fig 4b
Abcam Runx1 antibody (Abcam, ab23980) was used in ChIP-Seq on human samples (fig 4b). Cell (2018) ncbi
domestic rabbit polyclonal
  • ChIP-Seq; mouse; loading ...; fig s5b
Abcam Runx1 antibody (Abcam, ab23980) was used in ChIP-Seq on mouse samples (fig s5b). Nat Commun (2017) ncbi
domestic rabbit polyclonal
  • ChIP-Seq; mouse; loading ...; fig 5b
Abcam Runx1 antibody (Abcam, ab23980) was used in ChIP-Seq on mouse samples (fig 5b). Leukemia (2018) ncbi
domestic rabbit polyclonal
  • chromatin immunoprecipitation; mouse; loading ...; fig 1a
In order to study the role of RUNX1 and FOXP3 interplay in expression of breast cancer related genes, Abcam Runx1 antibody (Abcam, 23980) was used in chromatin immunoprecipitation on mouse samples (fig 1a). Oncotarget (2016) ncbi
Santa Cruz Biotechnology
mouse monoclonal (A-2)
  • immunocytochemistry; zebrafish ; loading ...; fig s9f
Santa Cruz Biotechnology Runx1 antibody (Santa, sc-365644) was used in immunocytochemistry on zebrafish samples (fig s9f). Science (2019) ncbi
mouse monoclonal (A-2)
  • western blot; human; loading ...; fig 3c
Santa Cruz Biotechnology Runx1 antibody (Santa Cruz, sc- 365644) was used in western blot on human samples (fig 3c). Blood Adv (2018) ncbi
mouse monoclonal (A-2)
  • immunohistochemistry - frozen section; mouse; loading ...; fig 2e
In order to utilize a G2-Gata4Cre;R26REYFP mouse line to track the developmental fate of the G2-Gata4 cell lineage, Santa Cruz Biotechnology Runx1 antibody (Santa Cruz Biotechnology, Sc-365644) was used in immunohistochemistry - frozen section on mouse samples (fig 2e). Haematologica (2017) ncbi
mouse monoclonal (A-2)
  • western blot; mouse
Santa Cruz Biotechnology Runx1 antibody (Santa Cruz, sc-365644) was used in western blot on mouse samples . J Biol Chem (2015) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D4A6)
  • western blot knockout validation; mouse; loading ...; fig 3c, 7d
Cell Signaling Technology Runx1 antibody (Cell signaling, 8529s) was used in western blot knockout validation on mouse samples (fig 3c, 7d). PLoS Genet (2021) ncbi
domestic rabbit monoclonal (D4A6)
  • immunohistochemistry; mouse; 1:100; loading ...; fig 1a
Cell Signaling Technology Runx1 antibody (Cell Signaling, 8529) was used in immunohistochemistry on mouse samples at 1:100 (fig 1a). elife (2020) ncbi
domestic rabbit monoclonal (D4A6)
  • immunocytochemistry; human
  • western blot; human
In order to identify DUBA as a suppressor of IL-17 production in T cells, Cell Signaling Technology Runx1 antibody (Cell Signaling, D4A6) was used in immunocytochemistry on human samples and in western blot on human samples . Nature (2015) ncbi
Articles Reviewed
  1. Malik N, Yan H, Yang H, Ayaz G, DuBois W, Tseng Y, et al. CBFB cooperates with p53 to maintain TAp73 expression and suppress breast cancer. PLoS Genet. 2021;17:e1009553 pubmed publisher
  2. Tang C, Wu M, Zhao D, Edwards D, McVicar A, Luo Y, et al. Runx1 is a central regulator of osteogenesis for bone homeostasis by orchestrating BMP and WNT signaling pathways. PLoS Genet. 2021;17:e1009233 pubmed publisher
  3. Mevel R, Steiner I, Mason S, Galbraith L, Patel R, Fadlullah M, et al. RUNX1 marks a luminal castration-resistant lineage established at the onset of prostate development. elife. 2020;9: pubmed publisher
  4. Yano F, Ohba S, Murahashi Y, Tanaka S, Saito T, Chung U. Runx1 contributes to articular cartilage maintenance by enhancement of cartilage matrix production and suppression of hypertrophic differentiation. Sci Rep. 2019;9:7666 pubmed publisher
  5. Malik N, Yan H, Moshkovich N, Palangat M, Yang H, Sanchez V, et al. The transcription factor CBFB suppresses breast cancer through orchestrating translation and transcription. Nat Commun. 2019;10:2071 pubmed publisher
  6. Gu Q, Yang X, Lv J, Zhang J, Xia B, Kim J, et al. AIBP-mediated cholesterol efflux instructs hematopoietic stem and progenitor cell fate. Science. 2019;363:1085-1088 pubmed publisher
  7. Pulikkan J, Hegde M, Ahmad H, Belaghzal H, Illendula A, Yu J, et al. CBFβ-SMMHC Inhibition Triggers Apoptosis by Disrupting MYC Chromatin Dynamics in Acute Myeloid Leukemia. Cell. 2018;174:172-186.e21 pubmed publisher
  8. Jalagadugula G, Goldfinger L, Mao G, Lambert M, Rao A. Defective RAB1B-related megakaryocytic ER-to-Golgi transport in RUNX1 haplodeficiency: impact on von Willebrand factor. Blood Adv. 2018;2:797-806 pubmed publisher
  9. Mao A, Ishizuka I, Kasal D, Mandal M, Bendelac A. A shared Runx1-bound Zbtb16 enhancer directs innate and innate-like lymphoid lineage development. Nat Commun. 2017;8:863 pubmed publisher
  10. Mingay M, Chaturvedi A, Bilenky M, Cao Q, Jackson L, Hui T, et al. Vitamin C-induced epigenomic remodelling in IDH1 mutant acute myeloid leukaemia. Leukemia. 2018;32:11-20 pubmed publisher
  11. Cañete A, Carmona R, Ariza L, Sanchez M, Rojas A, Muñoz Chápuli R. A population of hematopoietic stem cells derives from GATA4-expressing progenitors located in the placenta and lateral mesoderm of mice. Haematologica. 2017;102:647-655 pubmed publisher
  12. Recouvreux M, Grasso E, Echeverria P, Rocha Viegas L, Castilla L, Schere Levy C, et al. RUNX1 and FOXP3 interplay regulates expression of breast cancer related genes. Oncotarget. 2016;7:6552-65 pubmed publisher
  13. Majumder A, Syed K, Joseph S, Scambler P, Dutta D. Histone Chaperone HIRA in Regulation of Transcription Factor RUNX1. J Biol Chem. 2015;290:13053-63 pubmed publisher
  14. Rutz S, Kayagaki N, Phung Q, Eidenschenk C, Noubade R, Wang X, et al. Deubiquitinase DUBA is a post-translational brake on interleukin-17 production in T cells. Nature. 2015;518:417-21 pubmed publisher