This is a Validated Antibody Database (VAD) review about rat Rad50, based on 8 published articles (read how Labome selects the articles), using Rad50 antibody in all methods. It is aimed to help Labome visitors find the most suited Rad50 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Rad50 synonym: DNA repair protein RAD50; RAD50 homolog, double strand break repair protein

GeneTex
mouse monoclonal (13B3)
  • western blot; human; loading ...; fig 1f
GeneTex Rad50 antibody (Genetex, GTX70228) was used in western blot on human samples (fig 1f). Sci Signal (2018) ncbi
mouse monoclonal (13B3)
  • western blot; Escherichia coli; fig 6a
GeneTex Rad50 antibody (GeneTex, 13B3) was used in western blot on Escherichia coli samples (fig 6a). Nucleic Acids Res (2017) ncbi
mouse monoclonal (13B3)
  • immunoprecipitation; human; fig 3
  • immunocytochemistry; human; 1:200; fig 3
  • western blot; human; 1:1000; fig 3
GeneTex Rad50 antibody (Genetex, GTX70228) was used in immunoprecipitation on human samples (fig 3), in immunocytochemistry on human samples at 1:200 (fig 3) and in western blot on human samples at 1:1000 (fig 3). Nat Commun (2015) ncbi
mouse monoclonal (13B3)
  • western blot; human; fig 5
GeneTex Rad50 antibody (GeneTex, GTX70228) was used in western blot on human samples (fig 5). BMC Biol (2015) ncbi
mouse monoclonal (13B3)
  • chromatin immunoprecipitation; mouse
  • western blot; mouse
GeneTex Rad50 antibody (GeneTex, GTX70228) was used in chromatin immunoprecipitation on mouse samples and in western blot on mouse samples . J Cell Biol (2015) ncbi
mouse monoclonal (13B3)
  • western blot; human
GeneTex Rad50 antibody (GeneTex, #GTX70228) was used in western blot on human samples . Cancer Res (2011) ncbi
mouse monoclonal (13B3)
GeneTex Rad50 antibody (Genetex, GTX70228) was used . EMBO J (2010) ncbi
Abcam
rabbit monoclonal (EPR3466(2))
  • immunocytochemistry; mouse; 1:500; loading ...; fig s7j
  • western blot; mouse; 1:500; loading ...; fig s13a
  • western blot; human; 1:500; loading ...; fig s12e
Abcam Rad50 antibody (Abcam, ab124682) was used in immunocytochemistry on mouse samples at 1:500 (fig s7j), in western blot on mouse samples at 1:500 (fig s13a) and in western blot on human samples at 1:500 (fig s12e). Science (2016) ncbi
Articles Reviewed
  1. Lee J, Mand M, Kao C, Zhou Y, Ryu S, Richards A, et al. ATM directs DNA damage responses and proteostasis via genetically separable pathways. Sci Signal. 2018;11: pubmed publisher
  2. Deshpande R, Lee J, Paull T. Rad50 ATPase activity is regulated by DNA ends and requires coordination of both active sites. Nucleic Acids Res. 2017;45:5255-5268 pubmed publisher
  3. Kanakkanthara A, Jeganathan K, Limzerwala J, Baker D, Hamada M, Nam H, et al. Cyclin A2 is an RNA binding protein that controls Mre11 mRNA translation. Science. 2016;353:1549-1552 pubmed
  4. Lee K, Im J, Shibata E, Park J, Handa N, Kowalczykowski S, et al. MCM8-9 complex promotes resection of double-strand break ends by MRE11-RAD50-NBS1 complex. Nat Commun. 2015;6:7744 pubmed publisher
  5. Harten S, Oey H, Bourke L, Bharti V, Isbel L, Daxinger L, et al. The recently identified modifier of murine metastable epialleles, Rearranged L-Myc Fusion, is involved in maintaining epigenetic marks at CpG island shores and enhancers. BMC Biol. 2015;13:21 pubmed publisher
  6. Xiong J, Todorova D, Su N, Kim J, Lee P, Shen Z, et al. Stemness factor Sall4 is required for DNA damage response in embryonic stem cells. J Cell Biol. 2015;208:513-20 pubmed publisher
  7. Anderson V, Walton M, Eve P, Boxall K, Antoni L, Caldwell J, et al. CCT241533 is a potent and selective inhibitor of CHK2 that potentiates the cytotoxicity of PARP inhibitors. Cancer Res. 2011;71:463-72 pubmed publisher
  8. Lee J, Goodarzi A, Jeggo P, Paull T. 53BP1 promotes ATM activity through direct interactions with the MRN complex. EMBO J. 2010;29:574-85 pubmed publisher