This is a Validated Antibody Database (VAD) review about rat Ptbp1, based on 21 published articles (read how Labome selects the articles), using Ptbp1 antibody in all methods. It is aimed to help Labome visitors find the most suited Ptbp1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Ptbp1 synonym: PTB1; Ptb; Pybp; Pybp1; Pybp2; polypyrimidine tract-binding protein 1; heterogeneous nuclear ribonucleoprotein I; hnRNP I; pyrimidine binding protein 1; pyrimidine binding protein 2

Knockout validation
Invitrogen
mouse monoclonal (1)
  • western blot knockout validation; mouse; loading ...; fig 1c
In order to explore the role of heterogeneous nuclear ribonucleoprotein I in regulating neonatal immune adaptation, Invitrogen Ptbp1 antibody (Life Technologies, 324800) was used in western blot knockout validation on mouse samples (fig 1c). PLoS Genet (2017) ncbi
Invitrogen
mouse monoclonal (1)
  • western blot; human; 1:500; loading ...; fig 4c
Invitrogen Ptbp1 antibody (Thermo Fisher Scientific, 32-4800) was used in western blot on human samples at 1:500 (fig 4c). Mol Cell Biol (2018) ncbi
mouse monoclonal (1)
  • western blot; human; loading ...; fig 3a
Invitrogen Ptbp1 antibody (Invitrogen, 32-4800) was used in western blot on human samples (fig 3a). Nucleic Acids Res (2018) ncbi
mouse monoclonal (1)
  • western blot knockout validation; mouse; loading ...; fig 1c
In order to explore the role of heterogeneous nuclear ribonucleoprotein I in regulating neonatal immune adaptation, Invitrogen Ptbp1 antibody (Life Technologies, 324800) was used in western blot knockout validation on mouse samples (fig 1c). PLoS Genet (2017) ncbi
mouse monoclonal (1)
  • RNA immunoprecipitation; human; loading ...; fig 1d
  • western blot; human; loading ...; fig 1c
In order to study the functions of MEG3 and PTBP1 in human fibrotic and cirrhotic livers, Invitrogen Ptbp1 antibody (Thermo Fisher, 32-4800) was used in RNA immunoprecipitation on human samples (fig 1d) and in western blot on human samples (fig 1c). Hepatology (2017) ncbi
mouse monoclonal (1)
  • western blot; rat; fig 4
  • western blot; mouse; fig 7
In order to characterize the involvement of concerted non-productive splicing of post-transcriptional regulators that involves the alternative splicing program of differentiatied smooth muscle cells, Invitrogen Ptbp1 antibody (Invitrogen, 324800) was used in western blot on rat samples (fig 4) and in western blot on mouse samples (fig 7). Nucleic Acids Res (2016) ncbi
mouse monoclonal (1)
  • western blot; human; loading ...; fig 3e
In order to analyze the different isoforms of human CD46, Invitrogen Ptbp1 antibody (Life Technologies, 32-4800) was used in western blot on human samples (fig 3e). J Biol Chem (2016) ncbi
mouse monoclonal (1)
  • RNA immunoprecipitation; human; fig 1
In order to investigate how mitochondrial tRNAs affect cytoplasmic processes, Invitrogen Ptbp1 antibody (ThermoFisher Scientific, 32-4800) was used in RNA immunoprecipitation on human samples (fig 1). Nucleic Acids Res (2016) ncbi
mouse monoclonal (1)
  • western blot; human
In order to discuss methods for the characterization of stress granules in virus infected cells, Invitrogen Ptbp1 antibody (InvitroGen, 32-4800) was used in western blot on human samples . Methods (2015) ncbi
mouse monoclonal (1)
  • western blot; human; 1:1000; fig 5
In order to examine CD44 variants during the progression of melanoma to melanoma brain metastasis, Invitrogen Ptbp1 antibody (Invitrogen, 32-4800) was used in western blot on human samples at 1:1000 (fig 5). Pigment Cell Melanoma Res (2015) ncbi
mouse monoclonal (1)
  • western blot; human; fig 6
In order to report that MEF2 protein levels are regulated at the translational level and determine myocyte size during hypertrophy, Invitrogen Ptbp1 antibody (ZYMED, 324800) was used in western blot on human samples (fig 6). J Mol Cell Cardiol (2012) ncbi
mouse monoclonal (1)
  • western blot; mouse; 1:2000; fig 2
In order to study the role of polypyrimidine tract-binding protein in neuronal stem cells in the developing brain, Invitrogen Ptbp1 antibody (Zymed, 324800) was used in western blot on mouse samples at 1:2000 (fig 2). Cereb Cortex (2013) ncbi
mouse monoclonal (1)
  • western blot; mouse; fig 4
In order to study the mechanism for the tau exon mis-splicing in progressive supranuclear palsy, Invitrogen Ptbp1 antibody (Zymed, 32-4800) was used in western blot on mouse samples (fig 4). Hum Mol Genet (2011) ncbi
mouse monoclonal (1)
  • immunocytochemistry; human; 1:50; fig 5
In order to investigate stress granules assemble in cells infected with Theiler's murine encephalomyelitis virus, Invitrogen Ptbp1 antibody (Zymed, 32-4800) was used in immunocytochemistry on human samples at 1:50 (fig 5). J Virol (2011) ncbi
mouse monoclonal (1)
  • immunohistochemistry; mouse; fig 2
In order to use knock out mice to determine the role of polypyrimidine-tract binding protein 1 during development, Invitrogen Ptbp1 antibody (Zymed, 32-4800) was used in immunohistochemistry on mouse samples (fig 2). PLoS ONE (2011) ncbi
mouse monoclonal (1)
  • western blot; mouse; fig 2
In order to investigate the role of miRNAs in amyloid precursor protein splicing in vivo, Invitrogen Ptbp1 antibody (Zymed Laboratories, #32-4800) was used in western blot on mouse samples (fig 2). J Neurochem (2011) ncbi
mouse monoclonal (1)
  • immunocytochemistry; mouse; 1:50; fig 4
In order to compare viral leader proteins, Invitrogen Ptbp1 antibody (Zymed, 32-4800) was used in immunocytochemistry on mouse samples at 1:50 (fig 4). J Gen Virol (2006) ncbi
mouse monoclonal (1)
  • western blot; human; 1:250; fig 2
In order to study human cytomegalovirus UL37 isoforms, Invitrogen Ptbp1 antibody (Zymed, 32-4800) was used in western blot on human samples at 1:250 (fig 2). J Gen Virol (2004) ncbi
Abcam
goat polyclonal
  • western blot; human; fig 6
Abcam Ptbp1 antibody (abcam, ab5642) was used in western blot on human samples (fig 6). RNA (2016) ncbi
goat polyclonal
  • RNA immunoprecipitation; mouse; loading ...; fig s3d
Abcam Ptbp1 antibody (Abcam, ab5642) was used in RNA immunoprecipitation on mouse samples (fig s3d). Nature (2015) ncbi
Cell Signaling Technology
rabbit polyclonal
  • western blot; mouse; loading ...; fig s5d
In order to study the role of Yin-yang 2 in mouse embryonic stem cells, Cell Signaling Technology Ptbp1 antibody (Cell Signaling, 8776) was used in western blot on mouse samples (fig s5d). Proc Natl Acad Sci U S A (2016) ncbi
rabbit polyclonal
  • western blot; human; 1:1000
In order to describe the mechanisms by which hepatitis B virus core protein mediates resistance of hepatoma cells to apoptosis induced by anti-Fas antibody treatment, Cell Signaling Technology Ptbp1 antibody (Cell Signaling Technology, 8776) was used in western blot on human samples at 1:1000. FASEB J (2015) ncbi
Articles Reviewed
  1. Ghanem L, Kromer A, Silverman I, Ji X, Gazzara M, Nguyen N, et al. Poly(C)-Binding Protein Pcbp2 Enables Differentiation of Definitive Erythropoiesis by Directing Functional Splicing of the Runx1 Transcript. Mol Cell Biol. 2018;38: pubmed publisher
  2. Ji X, Humenik J, Yang D, Liebhaber S. PolyC-binding proteins enhance expression of the CDK2 cell cycle regulatory protein via alternative splicing. Nucleic Acids Res. 2018;46:2030-2044 pubmed publisher
  3. Jin Z, Liang F, Yang J, Mei W. hnRNP I regulates neonatal immune adaptation and prevents colitis and colorectal cancer. PLoS Genet. 2017;13:e1006672 pubmed publisher
  4. Tahmasebi S, Jafarnejad S, Tam I, Gonatopoulos Pournatzis T, Matta Camacho E, Tsukumo Y, et al. Control of embryonic stem cell self-renewal and differentiation via coordinated alternative splicing and translation of YY2. Proc Natl Acad Sci U S A. 2016;113:12360-12367 pubmed
  5. Zhang L, Yang Z, Trottier J, Barbier O, Wang L. Long noncoding RNA MEG3 induces cholestatic liver injury by interaction with PTBP1 to facilitate shp mRNA decay. Hepatology. 2017;65:604-615 pubmed publisher
  6. Llorian M, Gooding C, Bellora N, Hallegger M, Buckroyd A, Wang X, et al. The alternative splicing program of differentiated smooth muscle cells involves concerted non-productive splicing of post-transcriptional regulators. Nucleic Acids Res. 2016;44:8933-8950 pubmed
  7. Tang S, Luo S, Ho J, Ly P, Goh E, Roca X. Characterization of the Regulation of CD46 RNA Alternative Splicing. J Biol Chem. 2016;291:14311-23 pubmed publisher
  8. Kalantari R, Hicks J, Li L, Gagnon K, Sridhara V, Lemoff A, et al. Stable association of RNAi machinery is conserved between the cytoplasm and nucleus of human cells. RNA. 2016;22:1085-98 pubmed publisher
  9. Marnef A, Jády B, Kiss T. Human polypyrimidine tract-binding protein interacts with mitochondrial tRNA(Thr) in the cytosol. Nucleic Acids Res. 2016;44:1342-53 pubmed publisher
  10. McHugh C, Chen C, Chow A, Surka C, Tran C, McDonel P, et al. The Xist lncRNA interacts directly with SHARP to silence transcription through HDAC3. Nature. 2015;521:232-6 pubmed publisher
  11. Panas M, Kedersha N, McInerney G. Methods for the characterization of stress granules in virus infected cells. Methods. 2015;90:57-64 pubmed publisher
  12. Liu W, Lin Y, Yan X, Ding Y, Wu Y, Chen W, et al. Hepatitis B virus core protein inhibits Fas-mediated apoptosis of hepatoma cells via regulation of mFas/FasL and sFas expression. FASEB J. 2015;29:1113-23 pubmed publisher
  13. Marzese D, Liu M, Huynh J, Hirose H, Donovan N, Huynh K, et al. Brain metastasis is predetermined in early stages of cutaneous melanoma by CD44v6 expression through epigenetic regulation of the spliceosome. Pigment Cell Melanoma Res. 2015;28:82-93 pubmed publisher
  14. Ye J, Cardona M, Llovera M, Comella J, Sanchis D. Translation of Myocyte Enhancer Factor-2 is induced by hypertrophic stimuli in cardiomyocytes through a Calcineurin-dependent pathway. J Mol Cell Cardiol. 2012;53:578-87 pubmed publisher
  15. Shibasaki T, Tokunaga A, Sakamoto R, Sagara H, Noguchi S, Sasaoka T, et al. PTB deficiency causes the loss of adherens junctions in the dorsal telencephalon and leads to lethal hydrocephalus. Cereb Cortex. 2013;23:1824-35 pubmed publisher
  16. Smith P, Delay C, Girard J, Papon M, Planel E, Sergeant N, et al. MicroRNA-132 loss is associated with tau exon 10 inclusion in progressive supranuclear palsy. Hum Mol Genet. 2011;20:4016-24 pubmed publisher
  17. Borghese F, Michiels T. The leader protein of cardioviruses inhibits stress granule assembly. J Virol. 2011;85:9614-22 pubmed publisher
  18. Suckale J, Wendling O, Masjkur J, Jäger M, Münster C, Anastassiadis K, et al. PTBP1 is required for embryonic development before gastrulation. PLoS ONE. 2011;6:e16992 pubmed publisher
  19. Smith P, Al Hashimi A, Girard J, Delay C, Hébert S. In vivo regulation of amyloid precursor protein neuronal splicing by microRNAs. J Neurochem. 2011;116:240-7 pubmed publisher
  20. Paul S, Michiels T. Cardiovirus leader proteins are functionally interchangeable and have evolved to adapt to virus replication fitness. J Gen Virol. 2006;87:1237-46 pubmed
  21. Adair R, Liebisch G, Su Y, COLBERG POLEY A. Alteration of cellular RNA splicing and polyadenylation machineries during productive human cytomegalovirus infection. J Gen Virol. 2004;85:3541-53 pubmed