This is a Validated Antibody Database (VAD) review about rat Prmt5, based on 17 published articles (read how Labome selects the articles), using Prmt5 antibody in all methods. It is aimed to help Labome visitors find the most suited Prmt5 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Prmt5 synonym: Hrmt1l5; Skb1

Knockout validation
Abcam
domestic rabbit monoclonal (EPR5772)
  • immunohistochemistry knockout validation; mouse; 1:100; fig 2a
  • western blot knockout validation; mouse; 1:1000; fig 2c
  • immunohistochemistry - paraffin section; human; 1:100; loading ...; fig 1d
Abcam Prmt5 antibody (Abcam, ab109451) was used in immunohistochemistry knockout validation on mouse samples at 1:100 (fig 2a), in western blot knockout validation on mouse samples at 1:1000 (fig 2c) and in immunohistochemistry - paraffin section on human samples at 1:100 (fig 1d). Int J Biol Sci (2022) ncbi
Abcam
domestic rabbit monoclonal (EPR5772)
  • immunohistochemistry knockout validation; mouse; 1:100; fig 2a
  • western blot knockout validation; mouse; 1:1000; fig 2c
  • immunohistochemistry - paraffin section; human; 1:100; loading ...; fig 1d
Abcam Prmt5 antibody (Abcam, ab109451) was used in immunohistochemistry knockout validation on mouse samples at 1:100 (fig 2a), in western blot knockout validation on mouse samples at 1:1000 (fig 2c) and in immunohistochemistry - paraffin section on human samples at 1:100 (fig 1d). Int J Biol Sci (2022) ncbi
domestic rabbit monoclonal (EPR5772)
  • immunohistochemistry; human; 1:50; fig 1c
Abcam Prmt5 antibody (Abcam, ab109451) was used in immunohistochemistry on human samples at 1:50 (fig 1c). Theranostics (2021) ncbi
domestic rabbit monoclonal (EPR5772)
  • western blot; human; 1:5000; loading ...; fig s7
Abcam Prmt5 antibody (Abcam, ab109451) was used in western blot on human samples at 1:5000 (fig s7). Nat Commun (2020) ncbi
domestic rabbit monoclonal (EPR5772)
  • western blot; mouse; fig 1f
Abcam Prmt5 antibody (Abcam, ab109451) was used in western blot on mouse samples (fig 1f). Cell Rep (2019) ncbi
domestic rabbit monoclonal (EPR5772)
  • immunohistochemistry; human; loading ...; fig 1a
  • western blot; human; loading ...; fig 3a
Abcam Prmt5 antibody (Abcam, ab109451) was used in immunohistochemistry on human samples (fig 1a) and in western blot on human samples (fig 3a). Oncotarget (2016) ncbi
domestic rabbit monoclonal (EPR5772)
  • immunocytochemistry; mouse; 1:50; fig 5a
Abcam Prmt5 antibody (Abcam, ab109451) was used in immunocytochemistry on mouse samples at 1:50 (fig 5a). Genome Biol (2016) ncbi
domestic rabbit monoclonal (EPR5772)
  • western blot; mouse
Abcam Prmt5 antibody (Abcam, ab109451) was used in western blot on mouse samples . Mol Cancer Res (2013) ncbi
domestic rabbit monoclonal (EPR5772)
  • western blot; human
In order to investigate the role of menin in MEN1 tumor syndrome, Abcam Prmt5 antibody (Abcam, ab109451) was used in western blot on human samples . Cancer Res (2013) ncbi
Santa Cruz Biotechnology
mouse monoclonal (A-11)
  • western blot; human; fig s6c
Santa Cruz Biotechnology Prmt5 antibody (Santa Cruz, sc-376937) was used in western blot on human samples (fig s6c). Cancers (Basel) (2021) ncbi
mouse monoclonal (A-11)
  • immunohistochemistry - frozen section; mouse; loading ...; fig 2c
  • western blot; mouse; loading ...; fig 2a
Santa Cruz Biotechnology Prmt5 antibody (Santa Cruz Biotechnology Inc, sc-376937) was used in immunohistochemistry - frozen section on mouse samples (fig 2c) and in western blot on mouse samples (fig 2a). J Biol Chem (2018) ncbi
mouse monoclonal (A-11)
  • western blot; human; fig 6c
Santa Cruz Biotechnology Prmt5 antibody (Santa Cruz, sc-376937) was used in western blot on human samples (fig 6c). Nat Commun (2017) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot; human; 1:1000; fig s7
Cell Signaling Technology Prmt5 antibody (CST, 2252) was used in western blot on human samples at 1:1000 (fig s7). Nat Commun (2021) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 7c
Cell Signaling Technology Prmt5 antibody (Cell Signaling, 2252S) was used in western blot on human samples at 1:1000 (fig 7c). Nat Commun (2021) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 1b, 6a
Cell Signaling Technology Prmt5 antibody (Cell Signaling, 2252) was used in western blot on human samples (fig 1b, 6a). FASEB J (2018) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 1d
In order to examine the effects of the arginine methyltransferase PRMT5 on homologous recombination-mediated double-strand break repair, Cell Signaling Technology Prmt5 antibody (Cell Signaling Technologies, 2252) was used in western blot on human samples (fig 1d). Mol Cell (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; fig 1
Cell Signaling Technology Prmt5 antibody (CST, 2252) was used in western blot on human samples (fig 1). elife (2016) ncbi
MilliporeSigma
mouse monoclonal (PRMT5-21)
  • western blot; human; 1:1000; fig s3d
MilliporeSigma Prmt5 antibody (Sigma, P0493) was used in western blot on human samples at 1:1000 (fig s3d). BMC Cancer (2015) ncbi
Articles Reviewed
  1. Tang Y, Dong L, Zhang C, Li X, Li R, Lin H, et al. PRMT5 acts as a tumor suppressor by inhibiting Wnt/β-catenin signaling in murine gastric tumorigenesis. Int J Biol Sci. 2022;18:4329-4340 pubmed publisher
  2. Jiang Y, Yuan Y, Chen M, Li S, Bai J, Zhang Y, et al. PRMT5 disruption drives antitumor immunity in cervical cancer by reprogramming T cell-mediated response and regulating PD-L1 expression. Theranostics. 2021;11:9162-9176 pubmed publisher
  3. Giuliani V, Miller M, Liu C, Hartono S, Class C, Bristow C, et al. PRMT1-dependent regulation of RNA metabolism and DNA damage response sustains pancreatic ductal adenocarcinoma. Nat Commun. 2021;12:4626 pubmed publisher
  4. Bruce J, To K, Lui V, Chung G, Chan Y, Tsang C, et al. Whole-genome profiling of nasopharyngeal carcinoma reveals viral-host co-operation in inflammatory NF-κB activation and immune escape. Nat Commun. 2021;12:4193 pubmed publisher
  5. Szemes M, Melegh Z, Bellamy J, Park J, Chen B, Greenhough A, et al. Transcriptomic Analyses of MYCN-Regulated Genes in Anaplastic Wilms' Tumour Cell Lines Reveals Oncogenic Pathways and Potential Therapeutic Vulnerabilities. Cancers (Basel). 2021;13: pubmed publisher
  6. Szewczyk M, Ishikawa Y, Organ S, Sakai N, Li F, Halabelian L, et al. Pharmacological inhibition of PRMT7 links arginine monomethylation to the cellular stress response. Nat Commun. 2020;11:2396 pubmed publisher
  7. Tan D, Li Y, Yang C, Li J, Tan S, Chin D, et al. PRMT5 Modulates Splicing for Genome Integrity and Preserves Proteostasis of Hematopoietic Stem Cells. Cell Rep. 2019;26:2316-2328.e6 pubmed publisher
  8. Yang C, Chiu L, Chang C, Chuang H, Tan T. Induction of DUSP14 ubiquitination by PRMT5-mediated arginine methylation. FASEB J. 2018;:fj201800244RR pubmed publisher
  9. Huang L, Liu J, Zhang X, Sibley K, Najjar S, Lee M, et al. Inhibition of protein arginine methyltransferase 5 enhances hepatic mitochondrial biogenesis. J Biol Chem. 2018;293:10884-10894 pubmed publisher
  10. Wang Y, Liu X, Zhou L, Duong D, Bhuripanyo K, Zhao B, et al. Identifying the ubiquitination targets of E6AP by orthogonal ubiquitin transfer. Nat Commun. 2017;8:2232 pubmed publisher
  11. Clarke T, Sanchez Bailon M, Chiang K, Reynolds J, Herrero Ruiz J, Bandeiras T, et al. PRMT5-Dependent Methylation of the TIP60 Coactivator RUVBL1 Is a Key Regulator of Homologous Recombination. Mol Cell. 2017;65:900-916.e7 pubmed publisher
  12. Yang H, Zhao X, Zhao L, Liu L, Li J, Jia W, et al. PRMT5 competitively binds to CDK4 to promote G1-S transition upon glucose induction in hepatocellular carcinoma. Oncotarget. 2016;7:72131-72147 pubmed publisher
  13. Zhang X, Wu J, Wang J, Shen T, Li H, Lu J, et al. Integrative epigenomic analysis reveals unique epigenetic signatures involved in unipotency of mouse female germline stem cells. Genome Biol. 2016;17:162 pubmed publisher
  14. Mounir Z, Korn J, Westerling T, Lin F, Kirby C, Schirle M, et al. ERG signaling in prostate cancer is driven through PRMT5-dependent methylation of the Androgen Receptor. elife. 2016;5: pubmed publisher
  15. Rastetter R, Blömacher M, Drebber U, Marko M, Behrens J, Solga R, et al. Coronin 2A (CRN5) expression is associated with colorectal adenoma-adenocarcinoma sequence and oncogenic signalling. BMC Cancer. 2015;15:638 pubmed publisher
  16. Gurung B, Feng Z, Hua X. Menin directly represses Gli1 expression independent of canonical Hedgehog signaling. Mol Cancer Res. 2013;11:1215-22 pubmed publisher
  17. Gurung B, Feng Z, Iwamoto D, Thiel A, Jin G, Fan C, et al. Menin epigenetically represses Hedgehog signaling in MEN1 tumor syndrome. Cancer Res. 2013;73:2650-8 pubmed publisher