This is a Validated Antibody Database (VAD) review about rat Pfn1, based on 8 published articles (read how Labome selects the articles), using Pfn1 antibody in all methods. It is aimed to help Labome visitors find the most suited Pfn1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Pfn1 synonym: profilin-1; profilin I

Abcam
rabbit monoclonal (EPR6304)
  • immunohistochemistry - paraffin section; fruit fly; 1:100; loading ...
Abcam Pfn1 antibody (Abcam, ab124904) was used in immunohistochemistry - paraffin section on fruit fly samples at 1:100. J Biol Chem (2016) ncbi
rabbit monoclonal (EPR6304)
  • immunohistochemistry - paraffin section; human; 1:1000; fig 1
In order to assess the prognostic value of an immunohistochemistry-based three-protein signature model in patients with esophageal squamous cell carcinoma, Abcam Pfn1 antibody (Abcam, ab124904) was used in immunohistochemistry - paraffin section on human samples at 1:1000 (fig 1). Oncotarget (2015) ncbi
Proteintech Group
rabbit polyclonal
  • western blot; human; loading ...; fig 8
Proteintech Group Pfn1 antibody (Proteintech, 11680-1-AP) was used in western blot on human samples (fig 8). Oncotarget (2015) ncbi
Cell Signaling Technology
rabbit polyclonal
  • immunocytochemistry; mouse; 1:100; loading ...; fig 1d
  • western blot; human; loading ...; fig 7b
In order to elucidate the antitumor mechanism of Pfn1, Cell Signaling Technology Pfn1 antibody (Cell Signaling, 3237) was used in immunocytochemistry on mouse samples at 1:100 (fig 1d) and in western blot on human samples (fig 7b). J Biol Chem (2015) ncbi
Sigma-Aldrich
rabbit polyclonal
  • immunohistochemistry; mouse; 1:100; loading ...; fig 1a
  • western blot; mouse; 1:100; loading ...; fig 7c
Sigma-Aldrich Pfn1 antibody (Abcam, P7749) was used in immunohistochemistry on mouse samples at 1:100 (fig 1a) and in western blot on mouse samples at 1:100 (fig 7c). Mol Biol Cell (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 1b
In order to determine the display of unaltered actin binding and lack of affect on the actin cytoskeleton by profilin 1 with amyotrophic lateral sclerosis associated mutation T109M, Sigma-Aldrich Pfn1 antibody (Sigma-Aldrich, P7749) was used in western blot on human samples (fig 1b). BMC Neurosci (2015) ncbi
rabbit polyclonal
  • western blot; mouse; 1:500; fig 1
Sigma-Aldrich Pfn1 antibody (Sigma, P7624) was used in western blot on mouse samples at 1:500 (fig 1). PLoS ONE (2015) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:500; loading ...; fig 5
Sigma-Aldrich Pfn1 antibody (Sigma-Aldrich, AV48269) was used in immunohistochemistry - paraffin section on human samples at 1:500 (fig 5). Neurobiol Aging (2015) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:500; loading ...; fig 5
Sigma-Aldrich Pfn1 antibody (Sigma-Aldrich, P7624) was used in immunohistochemistry - paraffin section on human samples at 1:500 (fig 5). Neurobiol Aging (2015) ncbi
Articles Reviewed
  1. Matsukawa K, Hashimoto T, Matsumoto T, Ihara R, Chihara T, Miura M, et al. Familial Amyotrophic Lateral Sclerosis-linked Mutations in Profilin 1 Exacerbate TDP-43-induced Degeneration in the Retina of Drosophila melanogaster through an Increase in the Cytoplasmic Localization of TDP-43. J Biol Chem. 2016;291:23464-23476 pubmed
  2. Nejedlá M, Sadi S, Sulimenko V, de Almeida F, Blom H, Draber P, et al. Profilin connects actin assembly with microtubule dynamics. Mol Biol Cell. 2016;27:2381-93 pubmed publisher
  3. Freischmidt A, Schöpflin M, Feiler M, Fleck A, Ludolph A, Weishaupt J. Profilin 1 with the amyotrophic lateral sclerosis associated mutation T109M displays unaltered actin binding and does not affect the actin cytoskeleton. BMC Neurosci. 2015;16:77 pubmed publisher
  4. Bo H, Gong Z, Zhang W, Li X, Zeng Y, Liao Q, et al. Upregulated long non-coding RNA AFAP1-AS1 expression is associated with progression and poor prognosis of nasopharyngeal carcinoma. Oncotarget. 2015;6:20404-18 pubmed
  5. Checconi P, Salzano S, Bowler L, Mullen L, Mengozzi M, Hanschmann E, et al. Redox proteomics of the inflammatory secretome identifies a common set of redoxins and other glutathionylated proteins released in inflammation, influenza virus infection and oxidative stress. PLoS ONE. 2015;10:e0127086 pubmed publisher
  6. Diamond M, Cai S, Boudreau A, Carey C, Lyle N, Pappu R, et al. Subcellular localization and Ser-137 phosphorylation regulate tumor-suppressive activity of profilin-1. J Biol Chem. 2015;290:9075-86 pubmed publisher
  7. Cao H, Zhang S, SHEN J, Wu Z, Wu J, Wang S, et al. A three-protein signature and clinical outcome in esophageal squamous cell carcinoma. Oncotarget. 2015;6:5435-48 pubmed
  8. Smith B, Vance C, Scotter E, Troakes C, Wong C, Topp S, et al. Novel mutations support a role for Profilin 1 in the pathogenesis of ALS. Neurobiol Aging. 2015;36:1602.e17-27 pubmed publisher