This is a Validated Antibody Database (VAD) review about rat Opn4, based on 23 published articles (read how Labome selects the articles), using Opn4 antibody in all methods. It is aimed to help Labome visitors find the most suited Opn4 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Opn4 synonym: melanopsin

Invitrogen
rabbit polyclonal
  • immunohistochemistry; scFv; 1:1000; loading ...; fig 6e
Invitrogen Opn4 antibody (Thermo Fisher Scientific, PA1-781) was used in immunohistochemistry on scFv samples at 1:1000 (fig 6e). J Comp Neurol (2019) ncbi
rabbit polyclonal
  • immunocytochemistry; fruit fly; loading ...; fig 2
In order to determine that replacing the native Drosophila Rh1 photopigment of R1-6 photoreceptors with mouse OPN4 provides heterologous function, Invitrogen Opn4 antibody (ThermoFisher, PA1-780) was used in immunocytochemistry on fruit fly samples (fig 2). J Biol Chem (2017) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; rat; fig 1
In order to study the correlation between circadian rhythm impairments in sighted and blind rats and age-related changes in photosensitive melanopsin-expressing retinal ganglion cells, Invitrogen Opn4 antibody (Thermo Fisher Scientific, PA1-780) was used in immunohistochemistry - paraffin section on rat samples (fig 1). Chronobiol Int (2016) ncbi
rabbit polyclonal
  • immunohistochemistry; rat; 1:1000; fig 2
In order to study the dorsal raphe nucleus and afferents received by alpha-like retinal ganglion cells and photosensitive retinal ganglion cells in rats, Invitrogen Opn4 antibody (Thermo Fisher, PA1-780) was used in immunohistochemistry on rat samples at 1:1000 (fig 2). Invest Ophthalmol Vis Sci (2015) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse; 1:250; tbl 1
In order to compare the inhibition of dopamine amacrine cells and retinal ganglion cells in mouse retina, Invitrogen Opn4 antibody (Thermo Fisher, PA1-781) was used in immunohistochemistry on mouse samples at 1:250 (tbl 1). J Neurosci (2015) ncbi
rabbit polyclonal
  • immunohistochemistry; rat; 1:500; fig 8
In order to study the effects of ocular hypertension in limbar tissues, Invitrogen Opn4 antibody (Pierce, ThermoFisher, PA1-780) was used in immunohistochemistry on rat samples at 1:500 (fig 8). Prog Brain Res (2015) ncbi
rabbit polyclonal
  • immunocytochemistry; Mongolian gerbil; 1:200
In order to describe the organization of melanopsin-immunoreactive neurons in the Mongolian gerbil retina, Invitrogen Opn4 antibody (Thermo Scientific, PA1-781) was used in immunocytochemistry on Mongolian gerbil samples at 1:200. Neurosci Res (2015) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse; 1:5000
In order to assess the presence of ret-ret RGCs in juvenile and young adult rats and mice by using retrograde tracers applied to the contralateral optic nerve, Invitrogen Opn4 antibody (Pierce, PA1-780) was used in immunohistochemistry on mouse samples at 1:5000. Exp Eye Res (2015) ncbi
rabbit polyclonal
  • immunocytochemistry; rat; 1:1000
In order to examine the ganglion-cell photoreceptors in rat retina, Invitrogen Opn4 antibody (Thermo Fisher Scientificipore, PA1-780) was used in immunocytochemistry on rat samples at 1:1000. Exp Eye Res (2015) ncbi
rabbit polyclonal
  • immunocytochemistry; mouse; 1:200
In order to study light- and phosphorylation- dependent reduction in melanopsin signaling mediated by both beta-arrestin 1 and beta-arrestin 2, Invitrogen Opn4 antibody (Thermo Scientific, PA1-781) was used in immunocytochemistry on mouse samples at 1:200. PLoS ONE (2014) ncbi
rabbit polyclonal
  • immunohistochemistry; rat; 0.3 ug/ml
In order to improve formaldehyde-based fixatives to minimize bead formation of retinal ganglion cells, Invitrogen Opn4 antibody (Thermo, PA1-780) was used in immunohistochemistry on rat samples at 0.3 ug/ml. J Comp Neurol (2015) ncbi
rabbit polyclonal
  • immunohistochemistry; rat; 1:500; fig 3
In order to study the expression of the three Brn3 factors in the albino and pigmented adult rat, Invitrogen Opn4 antibody (Affynity Bioreagents, PA1-781) was used in immunohistochemistry on rat samples at 1:500 (fig 3). PLoS ONE (2012) ncbi
rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; 1:1000
  • immunohistochemistry; mouse; 1:1000
In order to study the heterogeneity of intrinsically photosensitive retinal ganglion cells in the mouse, Invitrogen Opn4 antibody (Affinity Bioreagents, PA1-781) was used in immunohistochemistry - frozen section on mouse samples at 1:1000 and in immunohistochemistry on mouse samples at 1:1000. J Comp Neurol (2013) ncbi
Abcam
rabbit polyclonal
  • western blot; mouse; 1:2000; fig 5
Abcam Opn4 antibody (Abcam, ab19306) was used in western blot on mouse samples at 1:2000 (fig 5). PLoS ONE (2016) ncbi
EMD Millipore
mouse monoclonal (RET-P1)
  • immunohistochemistry; common buzzard; 1:300; loading ...; tbl 1
In order to characterize photoreceptors in the raptor fovea, EMD Millipore Opn4 antibody (Millipore, MAB5316) was used in immunohistochemistry on common buzzard samples at 1:300 (tbl 1). J Comp Neurol (2017) ncbi
mouse monoclonal (RET-P1)
  • immunohistochemistry - paraffin section; rat; 1:2000; loading ...; fig st14
In order to outline the protocols for antibodies used for immunohistochemical studies, EMD Millipore Opn4 antibody (Millipore, MAB5316) was used in immunohistochemistry - paraffin section on rat samples at 1:2000 (fig st14). J Toxicol Pathol (2017) ncbi
mouse monoclonal (RET-P1)
  • immunohistochemistry; mouse; fig 5
In order to investigate regulation of the visual cycle genes Rdh5 and Rlbp1 in the retinal pigment epithelium by microphthalmia-associated transcription factor, EMD Millipore Opn4 antibody (Millipore, MAB5316) was used in immunohistochemistry on mouse samples (fig 5). Sci Rep (2016) ncbi
mouse monoclonal (RET-P1)
  • immunohistochemistry; rat; 1:100; fig s1
EMD Millipore Opn4 antibody (Millipore, MAB5316) was used in immunohistochemistry on rat samples at 1:100 (fig s1). Mol Ther (2016) ncbi
mouse monoclonal (RET-P1)
  • immunohistochemistry; fruit fly; 1:500; fig 2
EMD Millipore Opn4 antibody (Millipore, MAB 5316) was used in immunohistochemistry on fruit fly samples at 1:500 (fig 2). J Comp Neurol (2015) ncbi
mouse monoclonal (RET-P1)
  • immunohistochemistry - frozen section; dog; 1:200
  • immunohistochemistry - frozen section; mouse
EMD Millipore Opn4 antibody (Millipore, MAB5316) was used in immunohistochemistry - frozen section on dog samples at 1:200 and in immunohistochemistry - frozen section on mouse samples . PLoS ONE (2014) ncbi
mouse monoclonal (RET-P1)
  • immunocytochemistry; human
  • western blot; human
EMD Millipore Opn4 antibody (Millipore, MAB5316) was used in immunocytochemistry on human samples and in western blot on human samples . elife (2013) ncbi
mouse monoclonal (RET-P1)
  • immunohistochemistry; Rhesus monkey; 1:2000
EMD Millipore Opn4 antibody (Chemicon / Millipore, MAB5316) was used in immunohistochemistry on Rhesus monkey samples at 1:2000. Invest Ophthalmol Vis Sci (2013) ncbi
mouse monoclonal (RET-P1)
  • immunohistochemistry - frozen section; mouse; 1:10
In order to investigate the role of glycine receptor-mediated synaptic transmission in the maturation of ganglion cell synaptic connectivity, EMD Millipore Opn4 antibody (Chemicon, MAB5316) was used in immunohistochemistry - frozen section on mouse samples at 1:10. J Comp Neurol (2008) ncbi
Articles Reviewed
  1. Johnson E, Westbrook T, Shayesteh R, Chen E, Schumacher J, Fitzpatrick D, et al. Distribution and diversity of intrinsically photosensitive retinal ganglion cells in tree shrew. J Comp Neurol. 2019;527:328-344 pubmed publisher
  2. Mitkus M, Olsson P, Toomey M, Corbo J, Kelber A. Specialized photoreceptor composition in the raptor fovea. J Comp Neurol. 2017;525:2152-2163 pubmed publisher
  3. Furukawa S, Nagaike M, Ozaki K. Databases for technical aspects of immunohistochemistry. J Toxicol Pathol. 2017;30:79-107 pubmed publisher
  4. Yasin B, Kohn E, Peters M, Zaguri R, Weiss S, Schopf K, et al. Ectopic Expression of Mouse Melanopsin in Drosophila Photoreceptors Reveals Fast Response Kinetics and Persistent Dark Excitation. J Biol Chem. 2017;292:3624-3636 pubmed publisher
  5. Jain V, Srivastava I, Palchaudhuri S, Goel M, Sinha Mahapatra S, Dhingra N. Classical Photoreceptors Are Primarily Responsible for the Pupillary Light Reflex in Mouse. PLoS ONE. 2016;11:e0157226 pubmed publisher
  6. Lax P, Esquiva G, Fuentes Broto L, Segura F, Sanchez Cano A, Cuenca N, et al. Age-related changes in photosensitive melanopsin-expressing retinal ganglion cells correlate with circadian rhythm impairments in sighted and blind rats. Chronobiol Int. 2016;33:374-91 pubmed publisher
  7. Wen B, Li S, Li H, Chen Y, Ma X, Wang J, et al. Microphthalmia-associated transcription factor regulates the visual cycle genes Rlbp1 and Rdh5 in the retinal pigment epithelium. Sci Rep. 2016;6:21208 pubmed publisher
  8. Li X, Ren C, Huang L, Lin B, Pu M, Pickard G, et al. The Dorsal Raphe Nucleus Receives Afferents From Alpha-Like Retinal Ganglion Cells and Intrinsically Photosensitive Retinal Ganglion Cells in the Rat. Invest Ophthalmol Vis Sci. 2015;56:8373-81 pubmed publisher
  9. Bakondi B, Lv W, Lu B, Jones M, Tsai Y, Kim K, et al. In Vivo CRISPR/Cas9 Gene Editing Corrects Retinal Dystrophy in the S334ter-3 Rat Model of Autosomal Dominant Retinitis Pigmentosa. Mol Ther. 2016;24:556-63 pubmed publisher
  10. Vuong H, Hardi C, Barnes S, Brecha N. Parallel Inhibition of Dopamine Amacrine Cells and Intrinsically Photosensitive Retinal Ganglion Cells in a Non-Image-Forming Visual Circuit of the Mouse Retina. J Neurosci. 2015;35:15955-70 pubmed publisher
  11. Vidal Sanz M, Valiente Soriano F, Ortín Martínez A, Nadal Nicolás F, Jiménez López M, Salinas Navarro M, et al. Retinal neurodegeneration in experimental glaucoma. Prog Brain Res. 2015;220:1-35 pubmed publisher
  12. Jeong M, Jeon C. Localization of melanopsin-immunoreactive cells in the Mongolian gerbil retina. Neurosci Res. 2015;100:6-16 pubmed publisher
  13. Nadal Nicolás F, Valiente Soriano F, Salinas Navarro M, Jiménez López M, Vidal Sanz M, Agudo Barriuso M. Retino-retinal projection in juvenile and young adult rats and mice. Exp Eye Res. 2015;134:47-52 pubmed publisher
  14. Reifler A, Chervenak A, Dolikian M, Benenati B, Meyers B, Demertzis Z, et al. The rat retina has five types of ganglion-cell photoreceptors. Exp Eye Res. 2015;130:17-28 pubmed publisher
  15. Coimbra J, Collin S, Hart N. Variations in retinal photoreceptor topography and the organization of the rod-free zone reflect behavioral diversity in Australian passerines. J Comp Neurol. 2015;523:1073-94 pubmed publisher
  16. Cameron E, Robinson P. β-Arrestin-dependent deactivation of mouse melanopsin. PLoS ONE. 2014;9:e113138 pubmed publisher
  17. Stradleigh T, Greenberg K, Partida G, Pham A, Ishida A. Moniliform deformation of retinal ganglion cells by formaldehyde-based fixatives. J Comp Neurol. 2015;523:545-64 pubmed publisher
  18. Beltran W, Cideciyan A, Guziewicz K, Iwabe S, Swider M, Scott E, et al. Canine retina has a primate fovea-like bouquet of cone photoreceptors which is affected by inherited macular degenerations. PLoS ONE. 2014;9:e90390 pubmed publisher
  19. Tucker B, Mullins R, Streb L, Anfinson K, Eyestone M, Kaalberg E, et al. Patient-specific iPSC-derived photoreceptor precursor cells as a means to investigate retinitis pigmentosa. elife. 2013;2:e00824 pubmed publisher
  20. Dashek R, Kim C, Rasmussen C, Hennes Beean E, Ver Hoeve J, Nork T. Structural and functional effects of hemiretinal endodiathermy axotomy in cynomolgus macaques. Invest Ophthalmol Vis Sci. 2013;54:3479-92 pubmed publisher
  21. Nadal Nicolás F, Jiménez López M, Salinas Navarro M, Sobrado Calvo P, Alburquerque Béjar J, Vidal Sanz M, et al. Whole number, distribution and co-expression of brn3 transcription factors in retinal ganglion cells of adult albino and pigmented rats. PLoS ONE. 2012;7:e49830 pubmed publisher
  22. Karnas D, Mordel J, Bonnet D, Pevet P, Hicks D, Meissl H. Heterogeneity of intrinsically photosensitive retinal ganglion cells in the mouse revealed by molecular phenotyping. J Comp Neurol. 2013;521:912-32 pubmed publisher
  23. Xu H, Tian N. Glycine receptor-mediated synaptic transmission regulates the maturation of ganglion cell synaptic connectivity. J Comp Neurol. 2008;509:53-71 pubmed publisher