This is a Validated Antibody Database (VAD) review about rat Numa1, based on 5 published articles (read how Labome selects the articles), using Numa1 antibody in all methods. It is aimed to help Labome visitors find the most suited Numa1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Numa1 synonym: nuclear mitotic apparatus protein 1

Abcam
rabbit monoclonal (EP3976)
  • immunocytochemistry; human; 1:200; loading ...; fig 4d
Abcam Numa1 antibody (Abcam, ab109262) was used in immunocytochemistry on human samples at 1:200 (fig 4d). Sci Rep (2017) ncbi
rabbit monoclonal (EP3976)
  • immunoprecipitation; human; fig 8
  • immunocytochemistry; human; 1:500; fig 4
  • western blot; human; 1:1000; fig 8
In order to determine how Tank binding kinase 1 works in mitosis and microtubule dynamiccs, Abcam Numa1 antibody (Abcam, ab109262) was used in immunoprecipitation on human samples (fig 8), in immunocytochemistry on human samples at 1:500 (fig 4) and in western blot on human samples at 1:1000 (fig 8). Nat Commun (2015) ncbi
Novus Biologicals
rabbit polyclonal
  • immunocytochemistry; human; 1:100; loading ...; fig s5a
In order to determine that Light Intermediate Chain 2-dynein is a major mediator of proper spindle orientation and uncover its underlying molecular mechanism, Novus Biologicals Numa1 antibody (Novus Biologicals, NB500-174) was used in immunocytochemistry on human samples at 1:100 (fig s5a). Sci Rep (2016) ncbi
rabbit polyclonal
  • immunocytochemistry; human
In order to investigate the role of asymmetric cell division during tumorigenesis, Novus Biologicals Numa1 antibody (Novus Biologicals, NB500-174) was used in immunocytochemistry on human samples . Cancer Res (2014) ncbi
Invitrogen
rabbit polyclonal
  • immunocytochemistry; human; 1:1000; loading ...; fig s2c
In order to investigate that role of an ASPM-katanin complex in microtubule and spindle pole integrity, Invitrogen Numa1 antibody (Thermo Fisher, PA3-16829) was used in immunocytochemistry on human samples at 1:1000 (fig s2c). Nat Cell Biol (2017) ncbi
BD Biosciences
mouse monoclonal (22/NuMA)
  • immunoprecipitation; human; fig 5
  • western blot; human; 1:1000; fig 5
In order to determine how Tank binding kinase 1 works in mitosis and microtubule dynamiccs, BD Biosciences Numa1 antibody (BD Biosciences, 610561) was used in immunoprecipitation on human samples (fig 5) and in western blot on human samples at 1:1000 (fig 5). Nat Commun (2015) ncbi
Articles Reviewed
  1. Magescas J, Sengmanivong L, Viau A, Mayeux A, Dang T, Burtin M, et al. Spindle pole cohesion requires glycosylation-mediated localization of NuMA. Sci Rep. 2017;7:1474 pubmed publisher
  2. Jiang K, Rezabkova L, Hua S, Liu Q, Capitani G, Altelaar A, et al. Microtubule minus-end regulation at spindle poles by an ASPM-katanin complex. Nat Cell Biol. 2017;19:480-492 pubmed publisher
  3. Mahale S, Kumar M, Sharma A, Babu A, Ranjan S, Sachidanandan C, et al. The Light Intermediate Chain 2 Subpopulation of Dynein Regulates Mitotic Spindle Orientation. Sci Rep. 2016;6:22 pubmed publisher
  4. Pillai S, Nguyen J, Johnson J, Haura E, Coppola D, Chellappan S. Tank binding kinase 1 is a centrosome-associated kinase necessary for microtubule dynamics and mitosis. Nat Commun. 2015;6:10072 pubmed publisher
  5. Izumi H, Kaneko Y. Trim32 facilitates degradation of MYCN on spindle poles and induces asymmetric cell division in human neuroblastoma cells. Cancer Res. 2014;74:5620-30 pubmed publisher