This is a Validated Antibody Database (VAD) review about rat Npy, based on 25 published articles (read how Labome selects the articles), using Npy antibody in all methods. It is aimed to help Labome visitors find the most suited Npy antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Npy synonym: NPY02; RATNPY; RATNPY02

Knockout validation
MilliporeSigma
domestic rabbit polyclonal
  • immunohistochemistry knockout validation; zebrafish ; 1:5000; loading ...; fig 2
MilliporeSigma Npy antibody (Sigma-Aldrich, N9528) was used in immunohistochemistry knockout validation on zebrafish samples at 1:5000 (fig 2). Sci Rep (2020) ncbi
Abcam
domestic rabbit polyclonal
  • immunohistochemistry; human; 1:1000; loading ...; fig 13j
Abcam Npy antibody (ABCAM, ab30914) was used in immunohistochemistry on human samples at 1:1000 (fig 13j). Endocrinology (2021) ncbi
domestic rabbit polyclonal
  • western blot; rat; loading ...; fig 5s1
Abcam Npy antibody (Abcam, Ab30914) was used in western blot on rat samples (fig 5s1). elife (2020) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; 1:1000; loading ...; fig 4a
Abcam Npy antibody (Abcam, ab30914) was used in immunohistochemistry - frozen section on mouse samples at 1:1000 (fig 4a). Sci Rep (2020) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; mouse; 1:2000; loading ...; fig s4
Abcam Npy antibody (Abcam, AB30914) was used in immunohistochemistry on mouse samples at 1:2000 (fig s4). Nat Commun (2020) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; rat; 1:500; loading ...; fig 1a
Abcam Npy antibody (Abcam, ab30914) was used in immunohistochemistry - paraffin section on rat samples at 1:500 (fig 1a). J Ovarian Res (2020) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; rat; 1:2500; loading ...; fig 5d, 8d, 11d
Abcam Npy antibody (Abcam, ab30914) was used in immunohistochemistry - paraffin section on rat samples at 1:2500 (fig 5d, 8d, 11d). J Comp Neurol (2020) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - frozen section; degu; 1:10,000; loading ...; fig 7a
Abcam Npy antibody (Abcam, ab30914) was used in immunohistochemistry - frozen section on degu samples at 1:10,000 (fig 7a). J Comp Neurol (2019) ncbi
mouse monoclonal (8)
  • immunohistochemistry - frozen section; mouse; 1:5000; loading ...; fig 3a
Abcam Npy antibody (Abcam, ab112473) was used in immunohistochemistry - frozen section on mouse samples at 1:5000 (fig 3a). J Comp Neurol (2017) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; mouse; 1:10,000; loading ...; fig 5a
In order to elucidate the mechanism by which ghrelin activates paraventricular nucleus corticotropin-releasing factor neurons, Abcam Npy antibody (Abcam, ab30914) was used in immunohistochemistry on mouse samples at 1:10,000 (fig 5a). Psychoneuroendocrinology (2016) ncbi
Invitrogen
mouse monoclonal (8)
Invitrogen Npy antibody (ThermoFisher Scientific, ABS 028-08-02) was used . Animals (Basel) (2020) ncbi
mouse monoclonal (8)
  • immunohistochemistry - free floating section; rat; 1:500; fig 1d
In order to study how glucose and glutamate transporters are regulated by Ghrelin in hypothalamic astrocytes, Invitrogen Npy antibody (ThermoFisher, ABS 028-08-02) was used in immunohistochemistry - free floating section on rat samples at 1:500 (fig 1d). Sci Rep (2016) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D7Y5A)
  • immunohistochemistry; mouse; 1:1000; loading ...; fig 2j
Cell Signaling Technology Npy antibody (Cell Signaling, 11976S) was used in immunohistochemistry on mouse samples at 1:1000 (fig 2j). Nat Commun (2021) ncbi
domestic rabbit monoclonal (D7Y5A)
  • immunohistochemistry; mouse; 1:400; loading ...; fig 1c
Cell Signaling Technology Npy antibody (Cell Signaling Technology, 11976 s) was used in immunohistochemistry on mouse samples at 1:400 (fig 1c). elife (2021) ncbi
domestic rabbit monoclonal (D7Y5A)
  • immunohistochemistry - frozen section; mouse; 1:1000; loading ...; fig 5a
Cell Signaling Technology Npy antibody (Cell Signaling, 11976) was used in immunohistochemistry - frozen section on mouse samples at 1:1000 (fig 5a). Nat Commun (2020) ncbi
domestic rabbit monoclonal (D7Y5A)
  • western blot; rat; 1:1000; loading ...; fig 2a
Cell Signaling Technology Npy antibody (Cell Signaling, 11976) was used in western blot on rat samples at 1:1000 (fig 2a). J Ovarian Res (2020) ncbi
domestic rabbit monoclonal (D7Y5A)
  • immunohistochemistry; mouse; 1:1000; loading ...; fig 3k
Cell Signaling Technology Npy antibody (Cell Signaling, 11976) was used in immunohistochemistry on mouse samples at 1:1000 (fig 3k). elife (2019) ncbi
domestic rabbit monoclonal (D7Y5A)
  • immunohistochemistry; mouse; loading ...; fig st1
In order to develop a method for super-resolution imaging of the multiscale organization of intact tissues and use it to image the mouse brain, Cell Signaling Technology Npy antibody (Cell Signalling, 11976) was used in immunohistochemistry on mouse samples (fig st1). Nat Biotechnol (2016) ncbi
domestic rabbit monoclonal (D7Y5A)
  • immunohistochemistry - frozen section; rat; 1:1600; fig 5
Cell Signaling Technology Npy antibody (Cell signaling, 11976) was used in immunohistochemistry - frozen section on rat samples at 1:1600 (fig 5). Front Neurosci (2015) ncbi
domestic rabbit monoclonal (D7Y5A)
  • immunohistochemistry - frozen section; mouse; loading ...; fig 7h
Cell Signaling Technology Npy antibody (Millipore, 11976P) was used in immunohistochemistry - frozen section on mouse samples (fig 7h). PLoS ONE (2015) ncbi
Bachem
  • immunohistochemistry - frozen section; rat; 1:500-1:1000
Bachem Npy antibody (Bachem, IHC 7180) was used in immunohistochemistry - frozen section on rat samples at 1:500-1:1000. J Comp Neurol (2011) ncbi
MilliporeSigma
domestic rabbit polyclonal
  • immunohistochemistry - free floating section; rat; 1:1000; loading ...; fig 11d
MilliporeSigma Npy antibody (Sigma, N-9528) was used in immunohistochemistry - free floating section on rat samples at 1:1000 (fig 11d). Glia (2022) ncbi
domestic rabbit polyclonal
  • immunohistochemistry knockout validation; zebrafish ; 1:5000; loading ...; fig 2
MilliporeSigma Npy antibody (Sigma-Aldrich, N9528) was used in immunohistochemistry knockout validation on zebrafish samples at 1:5000 (fig 2). Sci Rep (2020) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; mouse; 1:500; loading ...; fig 5
MilliporeSigma Npy antibody (Sigma, N9528) was used in immunohistochemistry on mouse samples at 1:500 (fig 5). Dis Model Mech (2017) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; zebrafish ; 1:1000; loading ...; fig 4e
In order to describe the main afferents to the habenulae in larval and adult zebrafish, MilliporeSigma Npy antibody (Sigma, N9528) was used in immunohistochemistry on zebrafish samples at 1:1000 (fig 4e). Front Neural Circuits (2016) ncbi
domestic rabbit polyclonal
  • western blot; rat; loading ...; fig 6b
In order to examine the effects of dietary calcium supplementation and the mesocorticolimbic dopaminergic reward system in obese rats, MilliporeSigma Npy antibody (Sigma-Aldrich, N9528) was used in western blot on rat samples (fig 6b). J Neuroendocrinol (2016) ncbi
domestic rabbit polyclonal
MilliporeSigma Npy antibody (Sigma, N9528) was used . Biol Reprod (2015) ncbi
Articles Reviewed
  1. Fern xe1 ndez Arjona M, Le xf3 n Rodr xed guez A, Grondona J, L xf3 pez xc1 valos M. Long-term priming of hypothalamic microglia is associated with energy balance disturbances under diet-induced obesity. Glia. 2022;70:1734-1761 pubmed publisher
  2. Zhang X, Liu Y, Hong X, Li X, Meshul C, Moore C, et al. NG2 glia-derived GABA release tunes inhibitory synapses and contributes to stress-induced anxiety. Nat Commun. 2021;12:5740 pubmed publisher
  3. Lv X, Gao F, LI T, Xue P, Wang X, Wan M, et al. Skeleton interoception regulates bone and fat metabolism through hypothalamic neuroendocrine NPY. elife. 2021;10: pubmed publisher
  4. Keen K, Petersen A, Figueroa A, Fordyce B, Shin J, Yadav R, et al. Physiological Characterization and Transcriptomic Properties of GnRH Neurons Derived From Human Stem Cells. Endocrinology. 2021;162: pubmed publisher
  5. Kreutzberger A, Kiessling V, Doyle C, Schenk N, Upchurch C, Elmer Dixon M, et al. Distinct insulin granule subpopulations implicated in the secretory pathology of diabetes types 1 and 2. elife. 2020;9: pubmed publisher
  6. Jana B, Całka J. Endometritis Changes the Neurochemical Characteristics of the Caudal Mesenteric Ganglion Neurons Supplying the Gilt Uterus. Animals (Basel). 2020;10: pubmed publisher
  7. Shiozaki K, Kawabe M, Karasuyama K, Kurachi T, Hayashi A, Ataka K, et al. Neuropeptide Y deficiency induces anxiety-like behaviours in zebrafish (Danio rerio). Sci Rep. 2020;10:5913 pubmed publisher
  8. Ghule A, Racz I, Bilkei Gorzo A, Leidmaa E, Sieburg M, Zimmer A. Modulation of feeding behavior and metabolism by dynorphin. Sci Rep. 2020;10:3821 pubmed publisher
  9. Wu Z, Parry M, Hou X, Liu M, Wang H, Cain R, et al. Gene therapy conversion of striatal astrocytes into GABAergic neurons in mouse models of Huntington's disease. Nat Commun. 2020;11:1105 pubmed publisher
  10. Engström Ruud L, Pereira M, De Solis A, Fenselau H, Bruning J. NPY mediates the rapid feeding and glucose metabolism regulatory functions of AgRP neurons. Nat Commun. 2020;11:442 pubmed publisher
  11. Urata Y, Salehi R, Lima P, Osuga Y, Tsang B. Neuropeptide Y regulates proliferation and apoptosis in granulosa cells in a follicular stage-dependent manner. J Ovarian Res. 2020;13:5 pubmed publisher
  12. Carron S, Sun M, Shultz S, Rajan R. Inhibitory neuronal changes following a mixed diffuse-focal model of traumatic brain injury. J Comp Neurol. 2020;528:175-198 pubmed publisher
  13. Chen Y, Essner R, Kosar S, Miller O, Lin Y, Mesgarzadeh S, et al. Sustained NPY signaling enables AgRP neurons to drive feeding. elife. 2019;8: pubmed publisher
  14. Deichler A, Carrasco D, González Cabrera C, Letelier J, Mar n G, Mpodozis J. The nucleus pretectalis principalis: A pretectal structure hidden in the mammalian thalamus. J Comp Neurol. 2019;527:372-391 pubmed publisher
  15. Ladrón de Guevara Miranda D, Millón C, Rosell Valle C, Pérez Fernández M, Missiroli M, Serrano A, et al. Long-lasting memory deficits in mice withdrawn from cocaine are concomitant with neuroadaptations in hippocampal basal activity, GABAergic interneurons and adult neurogenesis. Dis Model Mech. 2017;10:323-336 pubmed publisher
  16. Yamada J, Jinno S. Molecular heterogeneity of aggrecan-based perineuronal nets around five subclasses of parvalbumin-expressing neurons in the mouse hippocampus. J Comp Neurol. 2017;525:1234-1249 pubmed publisher
  17. Ku T, Swaney J, Park J, Albanese A, Murray E, Cho J, et al. Multiplexed and scalable super-resolution imaging of three-dimensional protein localization in size-adjustable tissues. Nat Biotechnol. 2016;34:973-81 pubmed publisher
  18. Turner K, Hawkins T, Yáñez J, Anadón R, Wilson S, Folgueira M. Afferent Connectivity of the Zebrafish Habenulae. Front Neural Circuits. 2016;10:30 pubmed publisher
  19. Fuente Martín E, García Cáceres C, Argente Arizón P, Diaz F, Granado M, Freire Regatillo A, et al. Ghrelin Regulates Glucose and Glutamate Transporters in Hypothalamic Astrocytes. Sci Rep. 2016;6:23673 pubmed publisher
  20. Conceição E, Carvalho J, Manhães A, Guarda D, Figueiredo M, Quitete F, et al. Effect of Early Overfeeding on Palatable Food Preference and Brain Dopaminergic Reward System at Adulthood: Role of Calcium Supplementation. J Neuroendocrinol. 2016;28: pubmed publisher
  21. Cabral A, Portiansky E, Sánchez Jaramillo E, Zigman J, Perello M. Ghrelin activates hypophysiotropic corticotropin-releasing factor neurons independently of the arcuate nucleus. Psychoneuroendocrinology. 2016;67:27-39 pubmed publisher
  22. Lee S, Kang B, Shin M, Min J, Heo C, Lee Y, et al. Chronic Stress Decreases Cerebrovascular Responses During Rat Hindlimb Electrical Stimulation. Front Neurosci. 2015;9:462 pubmed publisher
  23. Johnson V, Xiang M, Chen Z, Junge H. Neurite Mistargeting and Inverse Order of Intraretinal Vascular Plexus Formation Precede Subretinal Vascularization in Vldlr Mutant Mice. PLoS ONE. 2015;10:e0132013 pubmed publisher
  24. Alves B, Cardoso R, Prezotto L, Thorson J, Bedenbaugh M, Sharpton S, et al. Elevated body weight gain during the juvenile period alters neuropeptide Y-gonadotropin-releasing hormone circuitry in prepubertal heifers. Biol Reprod. 2015;92:46 pubmed publisher
  25. Polgár E, Sardella T, Watanabe M, Todd A. Quantitative study of NPY-expressing GABAergic neurons and axons in rat spinal dorsal horn. J Comp Neurol. 2011;519:1007-23 pubmed publisher