This is a Validated Antibody Database (VAD) review about rat Nlgn1, based on 18 published articles (read how Labome selects the articles), using Nlgn1 antibody in all methods. It is aimed to help Labome visitors find the most suited Nlgn1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Knockout validation
Synaptic Systems
domestic rabbit polyclonal
  • western blot knockout validation; mouse; 1:2000; fig 1
  • immunocytochemistry; mouse; fig s1
Synaptic Systems Nlgn1 antibody (Synaptic System, 129013) was used in western blot knockout validation on mouse samples at 1:2000 (fig 1) and in immunocytochemistry on mouse samples (fig s1). J Cell Biol (2016) ncbi
Synaptic Systems
mouse monoclonal (87H9)
  • western blot knockout validation; mouse; 1:1000; fig 1
Synaptic Systems Nlgn1 antibody (Synaptic Systems, 129211) was used in western blot knockout validation on mouse samples at 1:1000 (fig 1). Front Synaptic Neurosci (2015) ncbi
Synaptic Systems
mouse monoclonal (4C12)
  • western blot; human; loading ...; fig 1a
Synaptic Systems Nlgn1 antibody (Synaptic Systems, 129,111) was used in western blot on human samples (fig 1a). Acta Neuropathol Commun (2021) ncbi
domestic rabbit polyclonal
  • immunoprecipitation; African green monkey; 1:1000; loading ...; fig 1c
  • western blot; African green monkey; 1:1000; loading ...; fig 1c
Synaptic Systems Nlgn1 antibody (Synaptic systems, 129013) was used in immunoprecipitation on African green monkey samples at 1:1000 (fig 1c) and in western blot on African green monkey samples at 1:1000 (fig 1c). elife (2020) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:2000; loading ...; fig 2s1c
Synaptic Systems Nlgn1 antibody (Synaptic Systems, 129013) was used in western blot on mouse samples at 1:2000 (fig 2s1c). elife (2020) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; fig 2c
Synaptic Systems Nlgn1 antibody (Synaptic Systems, 129013) was used in western blot on mouse samples at 1:1000 (fig 2c). Mol Neurobiol (2019) ncbi
mouse monoclonal (87H9)
  • immunohistochemistry; mouse; 1 ug/ml; loading ...; fig 6
  • immunohistochemistry; rat; 1 ug/ml; loading ...; tbl 1
In order to develop a novel synaptic junction preparation to characterize components of the synaptic cleft, Synaptic Systems Nlgn1 antibody (Synaptic Systems, 129 211) was used in immunohistochemistry on mouse samples at 1 ug/ml (fig 6) and in immunohistochemistry on rat samples at 1 ug/ml (tbl 1). PLoS ONE (2017) ncbi
mouse monoclonal (4C12)
  • western blot; mouse; 1:1000; fig s7e
Synaptic Systems Nlgn1 antibody (Synaptic Systems, 4C12) was used in western blot on mouse samples at 1:1000 (fig s7e). Proc Natl Acad Sci U S A (2017) ncbi
mouse monoclonal (4C12)
  • western blot; mouse; 1:500; fig 3
Synaptic Systems Nlgn1 antibody (Synaptic Systems, 129 111) was used in western blot on mouse samples at 1:500 (fig 3). PLoS ONE (2016) ncbi
mouse monoclonal (4F9)
  • western blot; mouse; fig 7
In order to analyze selective partitioning into complexes and supercomplexes during synapse maturation by NMDA receptors, Synaptic Systems Nlgn1 antibody (Synaptic Systems, 129 011) was used in western blot on mouse samples (fig 7). Nat Commun (2016) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; fig s4e
  • western blot; rat; 1:1000; fig s4a
Synaptic Systems Nlgn1 antibody (Synaptic Systems, 129013) was used in western blot on mouse samples at 1:1000 (fig s4e) and in western blot on rat samples at 1:1000 (fig s4a). Nat Commun (2016) ncbi
domestic rabbit polyclonal
  • western blot knockout validation; mouse; 1:2000; fig 1
  • immunocytochemistry; mouse; fig s1
Synaptic Systems Nlgn1 antibody (Synaptic System, 129013) was used in western blot knockout validation on mouse samples at 1:2000 (fig 1) and in immunocytochemistry on mouse samples (fig s1). J Cell Biol (2016) ncbi
mouse monoclonal (4C12)
  • western blot; mouse; fig 2
Synaptic Systems Nlgn1 antibody (Synaptic Systems, 4C12) was used in western blot on mouse samples (fig 2). elife (2016) ncbi
mouse monoclonal (87H9)
  • western blot knockout validation; mouse; 1:1000; fig 1
Synaptic Systems Nlgn1 antibody (Synaptic Systems, 129211) was used in western blot knockout validation on mouse samples at 1:1000 (fig 1). Front Synaptic Neurosci (2015) ncbi
mouse monoclonal (4F9)
  • western blot; rat; 1:1000; fig 2, 5
Synaptic Systems Nlgn1 antibody (Synaptic Systems, 129-011) was used in western blot on rat samples at 1:1000 (fig 2, 5). Biochem J (2016) ncbi
BioLegend
mouse monoclonal (N97A/31)
  • immunohistochemistry; mouse; loading ...; fig st1
In order to develop a method for super-resolution imaging of the multiscale organization of intact tissues and use it to image the mouse brain, BioLegend Nlgn1 antibody (BioLegend, 819001) was used in immunohistochemistry on mouse samples (fig st1). Nat Biotechnol (2016) ncbi
Neuromab
mouse monoclonal (N97A/31)
  • western blot; mouse; 1:300; loading ...; fig 4c
Neuromab Nlgn1 antibody (NeuroMab, 75-160) was used in western blot on mouse samples at 1:300 (fig 4c). Nat Commun (2017) ncbi
mouse monoclonal (N97A/31)
  • western blot; rat; 3.3 ug/ml; loading ...; tbl 1
In order to develop a novel synaptic junction preparation to characterize components of the synaptic cleft, Neuromab Nlgn1 antibody (NeuroMab, N97A/31) was used in western blot on rat samples at 3.3 ug/ml (tbl 1). PLoS ONE (2017) ncbi
mouse monoclonal (N97A/31)
  • western blot; rat; loading ...; fig 2b
Neuromab Nlgn1 antibody (NeuroMab, N97A/31) was used in western blot on rat samples (fig 2b). J Biol Chem (2016) ncbi
mouse monoclonal (N97A/31)
  • immunohistochemistry; mouse; 1:400
In order to study the role of down syndrome cell adhesion molecule during mice retina development, Neuromab Nlgn1 antibody (NeuroMab, 75-160) was used in immunohistochemistry on mouse samples at 1:400. Mol Vis (2014) ncbi
mouse monoclonal (N97A/31)
  • western blot; mouse; 1:1000
Neuromab Nlgn1 antibody (NeuroMab, N97A/31) was used in western blot on mouse samples at 1:1000. J Neurosci (2013) ncbi
Articles Reviewed
  1. Camporesi E, Lashley T, Gobom J, Lantero Rodriguez J, Hansson O, Zetterberg H, et al. Neuroligin-1 in brain and CSF of neurodegenerative disorders: investigation for synaptic biomarkers. Acta Neuropathol Commun. 2021;9:19 pubmed publisher
  2. Letellier M, Lagardère M, Tessier B, Janovjak H, Thoumine O. Optogenetic control of excitatory post-synaptic differentiation through neuroligin-1 tyrosine phosphorylation. elife. 2020;9: pubmed publisher
  3. Mastro T, Preza A, Basu S, Chattarji S, Till S, Kind P, et al. A sex difference in the response of the rodent postsynaptic density to synGAP haploinsufficiency. elife. 2020;9: pubmed publisher
  4. Chmielewska J, Kuzniewska B, Milek J, Urbanska K, Dziembowska M. Neuroligin 1, 2, and 3 Regulation at the Synapse: FMRP-Dependent Translation and Activity-Induced Proteolytic Cleavage. Mol Neurobiol. 2019;56:2741-2759 pubmed publisher
  5. Tanabe Y, Naito Y, Vasuta C, Lee A, Soumounou Y, Linhoff M, et al. IgSF21 promotes differentiation of inhibitory synapses via binding to neurexin2?. Nat Commun. 2017;8:408 pubmed publisher
  6. Burch A, Tao Cheng J, Dosemeci A. A novel synaptic junction preparation for the identification and characterization of cleft proteins. PLoS ONE. 2017;12:e0174895 pubmed publisher
  7. Sterky F, Trotter J, Lee S, Recktenwald C, Du X, Zhou B, et al. Carbonic anhydrase-related protein CA10 is an evolutionarily conserved pan-neurexin ligand. Proc Natl Acad Sci U S A. 2017;114:E1253-E1262 pubmed publisher
  8. Ku T, Swaney J, Park J, Albanese A, Murray E, Cho J, et al. Multiplexed and scalable super-resolution imaging of three-dimensional protein localization in size-adjustable tissues. Nat Biotechnol. 2016;34:973-81 pubmed publisher
  9. Mabb A, Simon J, KING I, Lee H, An L, Philpot B, et al. Topoisomerase 1 Regulates Gene Expression in Neurons through Cleavage Complex-Dependent and -Independent Mechanisms. PLoS ONE. 2016;11:e0156439 pubmed publisher
  10. Frank R, Komiyama N, Ryan T, Zhu F, O Dell T, Grant S. NMDA receptors are selectively partitioned into complexes and supercomplexes during synapse maturation. Nat Commun. 2016;7:11264 pubmed publisher
  11. Um J, Choii G, Park D, Kim D, Jeon S, Kang H, et al. IQ Motif and SEC7 Domain-containing Protein 3 (IQSEC3) Interacts with Gephyrin to Promote Inhibitory Synapse Formation. J Biol Chem. 2016;291:10119-30 pubmed publisher
  12. Chamma I, Letellier M, Butler C, Tessier B, Lim K, Gauthereau I, et al. Mapping the dynamics and nanoscale organization of synaptic adhesion proteins using monomeric streptavidin. Nat Commun. 2016;7:10773 pubmed publisher
  13. Liu A, Zhou Z, Dang R, Zhu Y, Qi J, He G, et al. Neuroligin 1 regulates spines and synaptic plasticity via LIMK1/cofilin-mediated actin reorganization. J Cell Biol. 2016;212:449-63 pubmed publisher
  14. Kuhn P, Colombo A, Schusser B, Dreymueller D, Wetzel S, Schepers U, et al. Systematic substrate identification indicates a central role for the metalloprotease ADAM10 in axon targeting and synapse function. elife. 2016;5: pubmed publisher
  15. Ye R, Quinlan M, Iwamoto H, Wu H, Green N, Jetter C, et al. Physical Interactions and Functional Relationships of Neuroligin 2 and Midbrain Serotonin Transporters. Front Synaptic Neurosci. 2015;7:20 pubmed publisher
  16. Ulbrich L, Favaloro F, Trobiani L, Marchetti V, Patel V, Pascucci T, et al. Autism-associated R451C mutation in neuroligin3 leads to activation of the unfolded protein response in a PC12 Tet-On inducible system. Biochem J. 2016;473:423-34 pubmed publisher
  17. de Andrade G, Kunzelman L, Merrill M, Fuerst P. Developmentally dynamic colocalization patterns of DSCAM with adhesion and synaptic proteins in the mouse retina. Mol Vis. 2014;20:1422-33 pubmed
  18. Hoy J, Haeger P, Constable J, Arias R, McCallum R, Kyweriga M, et al. Neuroligin1 drives synaptic and behavioral maturation through intracellular interactions. J Neurosci. 2013;33:9364-84 pubmed publisher