This is a Validated Antibody Database (VAD) review about rat Nkx6 1, based on 33 published articles (read how Labome selects the articles), using Nkx6 1 antibody in all methods. It is aimed to help Labome visitors find the most suited Nkx6 1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Nkx6 1 synonym: Nkx6.1; Nkx61; Nkx6a; homeobox protein Nkx-6.1; NK homeobox, family 6, A; NK6 transcription factor homolog A; NK6 transcription factor related, locus 1; homeobox protein NK-6 homolog A

Developmental Studies Hybridoma Bank
mouse monoclonal (F55A12)
  • immunohistochemistry - paraffin section; mouse; loading ...; fig s6j
Developmental Studies Hybridoma Bank Nkx6 1 antibody (DSHB, F55A12) was used in immunohistochemistry - paraffin section on mouse samples (fig s6j). Cell (2019) ncbi
mouse monoclonal (F55A12)
  • immunocytochemistry; mouse; loading ...; fig 3c
Developmental Studies Hybridoma Bank Nkx6 1 antibody (DSHB, F55A12) was used in immunocytochemistry on mouse samples (fig 3c). J Clin Invest (2019) ncbi
mouse monoclonal (F55A10)
  • immunocytochemistry; human; fig 2a
Developmental Studies Hybridoma Bank Nkx6 1 antibody (DSHB, F55A10) was used in immunocytochemistry on human samples (fig 2a). Cell (2018) ncbi
mouse monoclonal (F55A10)
  • flow cytometry; human; 1:2000; loading ...; tbl 1
In order to describe a four-stage differentiation protocol designed to generate pancreatic progenitor cells from human embryonic stem cells, Developmental Studies Hybridoma Bank Nkx6 1 antibody (DSHB, F55A10) was used in flow cytometry on human samples at 1:2000 (tbl 1). J Vis Exp (2017) ncbi
mouse monoclonal (F55A12)
  • flow cytometry; human; 1:250; fig 4b
  • immunocytochemistry; human; 1:500; fig 4e
Developmental Studies Hybridoma Bank Nkx6 1 antibody (DSHB, F55A12-c) was used in flow cytometry on human samples at 1:250 (fig 4b) and in immunocytochemistry on human samples at 1:500 (fig 4e). Cell Stem Cell (2017) ncbi
mouse monoclonal (F55A12)
  • immunohistochemistry; mouse; 1:25; loading ...; fig s2c
In order to study regulation of Notch1 in ventral neural stem/progenitor cells of the developing spinal cord, Developmental Studies Hybridoma Bank Nkx6 1 antibody (DSHB, F55A12) was used in immunohistochemistry on mouse samples at 1:25 (fig s2c). Sci Rep (2016) ncbi
mouse monoclonal (F55A12)
  • immunocytochemistry; human; 1:50; loading ...; tbl 1
In order to describe a method to produce human embryonic stem cell-derived Beta-like cells, Developmental Studies Hybridoma Bank Nkx6 1 antibody (Hybridoma Bank, F55A12) was used in immunocytochemistry on human samples at 1:50 (tbl 1). PLoS ONE (2016) ncbi
mouse monoclonal (F55A10)
  • immunohistochemistry - frozen section; mouse; fig 6
Developmental Studies Hybridoma Bank Nkx6 1 antibody (DSHB, F55A10) was used in immunohistochemistry - frozen section on mouse samples (fig 6). Mol Cell Neurosci (2016) ncbi
mouse monoclonal (F55A12)
  • immunohistochemistry - paraffin section; mouse; fig 6
In order to use a blinded study to examine the effects of ANGPTL8 upon beta-cell proliferation, Developmental Studies Hybridoma Bank Nkx6 1 antibody (DSHB, F55A12-c) was used in immunohistochemistry - paraffin section on mouse samples (fig 6). PLoS ONE (2016) ncbi
mouse monoclonal (F64A6B4)
  • immunohistochemistry; mouse; 1:100; loading ...; fig 3a
Developmental Studies Hybridoma Bank Nkx6 1 antibody (DSHB, F64A6B4) was used in immunohistochemistry on mouse samples at 1:100 (fig 3a). FEBS Lett (2016) ncbi
mouse monoclonal (F65A2)
  • immunohistochemistry; mouse; fig s1
Developmental Studies Hybridoma Bank Nkx6 1 antibody (Developmental Studies Hybridoma Bank, F65A2) was used in immunohistochemistry on mouse samples (fig s1). PLoS ONE (2016) ncbi
mouse monoclonal (F55A12)
  • flow cytometry; human; 1:200; fig s19
Developmental Studies Hybridoma Bank Nkx6 1 antibody (Developmental Studies Hybridoma Bank, F55A12-s) was used in flow cytometry on human samples at 1:200 (fig s19). Nat Commun (2016) ncbi
mouse monoclonal (F55A10)
  • immunocytochemistry; human; 1:300
Developmental Studies Hybridoma Bank Nkx6 1 antibody (DSHB, F55A10) was used in immunocytochemistry on human samples at 1:300. Nature (2016) ncbi
mouse monoclonal (F55A10)
  • immunohistochemistry; mouse; 1:50; fig 1
In order to analyze regulation of formation of the columnar neural epithelium by the tumor suppressor PTEN and the PDK1 kinase, Developmental Studies Hybridoma Bank Nkx6 1 antibody (Hybridoma Bank, F55A10) was used in immunohistochemistry on mouse samples at 1:50 (fig 1). elife (2016) ncbi
mouse monoclonal (F55A12)
  • immunohistochemistry - paraffin section; human; fig 3
Developmental Studies Hybridoma Bank Nkx6 1 antibody (DSHB, F55A12) was used in immunohistochemistry - paraffin section on human samples (fig 3). Nat Med (2016) ncbi
mouse monoclonal (F55A10)
  • immunohistochemistry; chicken; 1:20; fig 2
In order to assess and validate novel hedgehog-responsive enhancers by computational analysis prediction of Ci/Gli binding site density, Developmental Studies Hybridoma Bank Nkx6 1 antibody (DSHB, F55A10) was used in immunohistochemistry on chicken samples at 1:20 (fig 2). PLoS ONE (2015) ncbi
mouse monoclonal (F55A12)
  • immunohistochemistry - frozen section; zebrafish ; 1:500; fig 3
Developmental Studies Hybridoma Bank Nkx6 1 antibody (DSHB, F55A12) was used in immunohistochemistry - frozen section on zebrafish samples at 1:500 (fig 3). Genes Dev (2015) ncbi
mouse monoclonal (F55A10)
  • immunohistochemistry; mouse; 1:50; fig 4
In order to characterize sonic hedgehog-mediated dopaminergic neurogenesis in the embryonic midbrain by primary cilia, Developmental Studies Hybridoma Bank Nkx6 1 antibody (Developmental Studies Hybridoma Bank, F55A10) was used in immunohistochemistry on mouse samples at 1:50 (fig 4). Dev Biol (2016) ncbi
mouse monoclonal (F55A10)
  • flow cytometry; human; 1:2000; fig 3
In order to describe protocols to generate specific pancreatic lineages from hPSCs, Developmental Studies Hybridoma Bank Nkx6 1 antibody (DSHB, F55A10) was used in flow cytometry on human samples at 1:2000 (fig 3). Methods (2016) ncbi
mouse monoclonal (F55A10)
  • immunohistochemistry - paraffin section; human; 1:100; fig 3
In order to analyze a model for ductal pancreatic cancer and drug screening, Developmental Studies Hybridoma Bank Nkx6 1 antibody (Developmental Studies Hybridoma Bank, F55A10) was used in immunohistochemistry - paraffin section on human samples at 1:100 (fig 3). Nat Med (2015) ncbi
mouse monoclonal (F55A10)
  • immunocytochemistry; human; 1:200; fig 4a
In order to demonstrate that dCas9 activator controls human pluripotent stem cell differentiation into endodermal lineages, Developmental Studies Hybridoma Bank Nkx6 1 antibody (DSHB, F55A10) was used in immunocytochemistry on human samples at 1:200 (fig 4a). Stem Cell Reports (2015) ncbi
mouse monoclonal (F55A10)
  • immunohistochemistry; human; 1:20; fig 1
In order to assess dorsal root avulsion injury and how human embryonic stem cell-derived progenitors assist functional sensory axo regeneration, Developmental Studies Hybridoma Bank Nkx6 1 antibody (DSHB, F55A10) was used in immunohistochemistry on human samples at 1:20 (fig 1). Sci Rep (2015) ncbi
mouse monoclonal (F55A10)
  • immunohistochemistry; mouse; fig s5
In order to study Shh signaling during oncogenesis and hindbrain development due to Eya1 phosphate promotion, Developmental Studies Hybridoma Bank Nkx6 1 antibody (Developmental Studies Hybridoma Bank, F55A10) was used in immunohistochemistry on mouse samples (fig s5). Dev Cell (2015) ncbi
mouse monoclonal (F55A10)
  • immunohistochemistry; mouse; 1:50; fig s5
In order to test if extracellular signals orient the mitotic spindle of cells in the spinal cord, Developmental Studies Hybridoma Bank Nkx6 1 antibody (Developmental Studies Hybridoma Bank, F55A10) was used in immunohistochemistry on mouse samples at 1:50 (fig s5). Nat Commun (2015) ncbi
mouse monoclonal (F55A12)
  • immunocytochemistry; mouse
  • western blot; mouse
Developmental Studies Hybridoma Bank Nkx6 1 antibody (DHSB, F55A12-c) was used in immunocytochemistry on mouse samples and in western blot on mouse samples . Nat Biotechnol (2014) ncbi
mouse monoclonal (F55A12)
  • immunocytochemistry; human; 1:100; fig 5
In order to describe a scalable, automated approach to test the ability of small molecules to promote human pluripotent stem cell differentiation, Developmental Studies Hybridoma Bank Nkx6 1 antibody (DSHB, F55A12) was used in immunocytochemistry on human samples at 1:100 (fig 5). Nat Biotechnol (2015) ncbi
mouse monoclonal (F55A12)
  • immunohistochemistry - paraffin section; mouse; 1:50
In order to investigate the function of islet-1 in the pancreas, Developmental Studies Hybridoma Bank Nkx6 1 antibody (Hybridoma Bank, F55A12-C) was used in immunohistochemistry - paraffin section on mouse samples at 1:50. Diabetes (2014) ncbi
mouse monoclonal (F55A10)
  • immunohistochemistry - paraffin section; mouse; 1:200; fig 3
Developmental Studies Hybridoma Bank Nkx6 1 antibody (DSHB, F55A10) was used in immunohistochemistry - paraffin section on mouse samples at 1:200 (fig 3). Nat Commun (2014) ncbi
mouse monoclonal (F55A10)
  • immunocytochemistry; mouse; 1:100
Developmental Studies Hybridoma Bank Nkx6 1 antibody (DSHB, F55A10) was used in immunocytochemistry on mouse samples at 1:100. Neurotoxicol Teratol (2014) ncbi
mouse monoclonal (F55A10)
  • immunohistochemistry - frozen section; chicken; 1:20
In order to study the role of Sox2 activity in neural differentiation, Developmental Studies Hybridoma Bank Nkx6 1 antibody (Developmental Studies Hybridoma Bank, F55A10) was used in immunohistochemistry - frozen section on chicken samples at 1:20. Proc Natl Acad Sci U S A (2014) ncbi
mouse monoclonal (F55A10)
  • immunohistochemistry - frozen section; chicken
Developmental Studies Hybridoma Bank Nkx6 1 antibody (DSHB, F55A10) was used in immunohistochemistry - frozen section on chicken samples . PLoS ONE (2014) ncbi
mouse monoclonal (F55A10)
  • immunohistochemistry - paraffin section; mouse
Developmental Studies Hybridoma Bank Nkx6 1 antibody (Dev. Studies Hybridoma Bank, F55A10) was used in immunohistochemistry - paraffin section on mouse samples . PLoS ONE (2013) ncbi
mouse monoclonal (F55A10)
  • immunohistochemistry - free floating section; Spanish newt; 1:500
Developmental Studies Hybridoma Bank Nkx6 1 antibody (Developmental Studies Hybridoma Bank, F55A10) was used in immunohistochemistry - free floating section on Spanish newt samples at 1:500. J Comp Neurol (2013) ncbi
Articles Reviewed
  1. Sharon N, Chawla R, Mueller J, Vanderhooft J, Whitehorn L, Rosenthal B, et al. A Peninsular Structure Coordinates Asynchronous Differentiation with Morphogenesis to Generate Pancreatic Islets. Cell. 2019;176:790-804.e13 pubmed publisher
  2. Bartolomé A, Zhu C, Sussel L, Pajvani U. Notch signaling dynamically regulates adult β cell proliferation and maturity. J Clin Invest. 2019;129:268-280 pubmed publisher
  3. Wu X, Dao Thi V, Huang Y, Billerbeck E, Saha D, Hoffmann H, et al. Intrinsic Immunity Shapes Viral Resistance of Stem Cells. Cell. 2018;172:423-438.e25 pubmed publisher
  4. McGaugh E, Nostro M. Efficient Differentiation of Pluripotent Stem Cells to NKX6-1+ Pancreatic Progenitors. J Vis Exp. 2017;: pubmed publisher
  5. Shi Z, Lee K, Yang D, Amin S, Verma N, Li Q, et al. Genome Editing in hPSCs Reveals GATA6 Haploinsufficiency and a Genetic Interaction with GATA4 in Human Pancreatic Development. Cell Stem Cell. 2017;20:675-688.e6 pubmed publisher
  6. Li Y, Tzatzalos E, Kwan K, Grumet M, Cai L. Transcriptional Regulation of Notch1 Expression by Nkx6.1 in Neural Stem/Progenitor Cells during Ventral Spinal Cord Development. Sci Rep. 2016;6:38665 pubmed publisher
  7. Massumi M, Pourasgari F, Nalla A, Batchuluun B, Nagy K, Neely E, et al. An Abbreviated Protocol for In Vitro Generation of Functional Human Embryonic Stem Cell-Derived Beta-Like Cells. PLoS ONE. 2016;11:e0164457 pubmed publisher
  8. Fogarty L, Song B, Suppiah Y, Hasan S, Martin H, Hogan S, et al. Bcl-xL dependency coincides with the onset of neurogenesis in the developing mammalian spinal cord. Mol Cell Neurosci. 2016;77:34-46 pubmed publisher
  9. Cox A, Barrandon O, Cai E, Rios J, Chavez J, Bonnyman C, et al. Resolving Discrepant Findings on ANGPTL8 in ?-Cell Proliferation: A Collaborative Approach to Resolving the Betatrophin Controversy. PLoS ONE. 2016;11:e0159276 pubmed publisher
  10. Omori H, Ogaki S, Sakano D, Sato M, Umeda K, Takeda N, et al. Changes in expression of C2cd4c in pancreatic endocrine cells during pancreatic development. FEBS Lett. 2016;590:2584-93 pubmed publisher
  11. Airik R, Schueler M, Airik M, Cho J, Ulanowicz K, Porath J, et al. SDCCAG8 Interacts with RAB Effector Proteins RABEP2 and ERC1 and Is Required for Hedgehog Signaling. PLoS ONE. 2016;11:e0156081 pubmed publisher
  12. Saxena P, Heng B, Bai P, Folcher M, Zulewski H, Fussenegger M. A programmable synthetic lineage-control network that differentiates human IPSCs into glucose-sensitive insulin-secreting beta-like cells. Nat Commun. 2016;7:11247 pubmed publisher
  13. Sagi I, Chia G, Golan Lev T, Peretz M, Weissbein U, Sui L, et al. Derivation and differentiation of haploid human embryonic stem cells. Nature. 2016;532:107-11 pubmed publisher
  14. Grego Bessa J, Bloomekatz J, Castel P, Omelchenko T, Baselga J, Anderson K. The tumor suppressor PTEN and the PDK1 kinase regulate formation of the columnar neural epithelium. elife. 2016;5:e12034 pubmed publisher
  15. Vegas A, Veiseh O, Gürtler M, Millman J, Pagliuca F, Bader A, et al. Long-term glycemic control using polymer-encapsulated human stem cell-derived beta cells in immune-competent mice. Nat Med. 2016;22:306-11 pubmed publisher
  16. Gurdziel K, Lorberbaum D, Udager A, Song J, Richards N, Parker D, et al. Identification and Validation of Novel Hedgehog-Responsive Enhancers Predicted by Computational Analysis of Ci/Gli Binding Site Density. PLoS ONE. 2015;10:e0145225 pubmed publisher
  17. Ravanelli A, Appel B. Motor neurons and oligodendrocytes arise from distinct cell lineages by progenitor recruitment. Genes Dev. 2015;29:2504-15 pubmed publisher
  18. Gazea M, Tasouri E, Tolve M, Bosch V, Kabanova A, Gojak C, et al. Primary cilia are critical for Sonic hedgehog-mediated dopaminergic neurogenesis in the embryonic midbrain. Dev Biol. 2016;409:55-71 pubmed publisher
  19. Korytnikov R, Nostro M. Generation of polyhormonal and multipotent pancreatic progenitor lineages from human pluripotent stem cells. Methods. 2016;101:56-64 pubmed publisher
  20. Huang L, Holtzinger A, Jagan I, BeGora M, Lohse I, Ngai N, et al. Ductal pancreatic cancer modeling and drug screening using human pluripotent stem cell- and patient-derived tumor organoids. Nat Med. 2015;21:1364-71 pubmed publisher
  21. Balboa D, Weltner J, Eurola S, Trokovic R, Wartiovaara K, Otonkoski T. Conditionally Stabilized dCas9 Activator for Controlling Gene Expression in Human Cell Reprogramming and Differentiation. Stem Cell Reports. 2015;5:448-59 pubmed publisher
  22. Hoeber J, Trolle C, König N, Du Z, Gallo A, Hermans E, et al. Human Embryonic Stem Cell-Derived Progenitors Assist Functional Sensory Axon Regeneration after Dorsal Root Avulsion Injury. Sci Rep. 2015;5:10666 pubmed publisher
  23. Eisner A, Pazyra Murphy M, Durresi E, Zhou P, Zhao X, Chadwick E, et al. The Eya1 phosphatase promotes Shh signaling during hindbrain development and oncogenesis. Dev Cell. 2015;33:22-35 pubmed publisher
  24. Arbeille E, Reynaud F, Sanyas I, Bozon M, Kindbeiter K, Causeret F, et al. Cerebrospinal fluid-derived Semaphorin3B orients neuroepithelial cell divisions in the apicobasal axis. Nat Commun. 2015;6:6366 pubmed publisher
  25. Li W, Cavelti Weder C, Zhang Y, Zhang Y, Clement K, Donovan S, et al. Long-term persistence and development of induced pancreatic beta cells generated by lineage conversion of acinar cells. Nat Biotechnol. 2014;32:1223-30 pubmed publisher
  26. Maury Y, Côme J, Piskorowski R, Salah Mohellibi N, Chevaleyre V, Peschanski M, et al. Combinatorial analysis of developmental cues efficiently converts human pluripotent stem cells into multiple neuronal subtypes. Nat Biotechnol. 2015;33:89-96 pubmed publisher
  27. Ediger B, Du A, Liu J, Hunter C, Walp E, Schug J, et al. Islet-1 Is essential for pancreatic ?-cell function. Diabetes. 2014;63:4206-17 pubmed publisher
  28. Veleri S, Manjunath S, Fariss R, May Simera H, Brooks M, Foskett T, et al. Ciliopathy-associated gene Cc2d2a promotes assembly of subdistal appendages on the mother centriole during cilia biogenesis. Nat Commun. 2014;5:4207 pubmed publisher
  29. Akeju O, Davis Dusenbery B, Cassel S, Ichida J, Eggan K. Ketamine exposure in early development impairs specification of the primary germ cell layers. Neurotoxicol Teratol. 2014;43:59-68 pubmed publisher
  30. Lee K, Seo J, Shin J, Ji E, Roh J, Kim J, et al. Positive feedback loop between Sox2 and Sox6 inhibits neuronal differentiation in the developing central nervous system. Proc Natl Acad Sci U S A. 2014;111:2794-9 pubmed publisher
  31. Yan X, Lin J, Talabattula V, Mußmann C, Yang F, Wree A, et al. ADAM10 negatively regulates neuronal differentiation during spinal cord development. PLoS ONE. 2014;9:e84617 pubmed publisher
  32. Diaz de Durana Y, Lau J, Knee D, Filippi C, Londei M, McNamara P, et al. IL-2 immunotherapy reveals potential for innate beta cell regeneration in the non-obese diabetic mouse model of autoimmune diabetes. PLoS ONE. 2013;8:e78483 pubmed publisher
  33. Joven A, Morona R, González A, Moreno N. Spatiotemporal patterns of Pax3, Pax6, and Pax7 expression in the developing brain of a urodele amphibian, Pleurodeles waltl. J Comp Neurol. 2013;521:3913-53 pubmed publisher