This is a Validated Antibody Database (VAD) review about rat Nf2, based on 15 published articles (read how Labome selects the articles), using Nf2 antibody in all methods. It is aimed to help Labome visitors find the most suited Nf2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Nf2 synonym: merlin

Knockout validation
Abcam
domestic rabbit monoclonal (EPR2573(2))
  • western blot knockout validation; human; 1:1000; fig 4
Abcam Nf2 antibody (Abcam, ab109244) was used in western blot knockout validation on human samples at 1:1000 (fig 4). Science (2014) ncbi
MilliporeSigma
domestic rabbit polyclonal
  • immunohistochemistry knockout validation; mouse; 1:300; fig 1
  • western blot; mouse; 1:2000; fig s3
In order to investigate the roles of NF2, LATS1/2, and YAP in the branching morphogenesis of the mouse kidney, MilliporeSigma Nf2 antibody (Sigma Prestige Antibodies, HPA003097) was used in immunohistochemistry knockout validation on mouse samples at 1:300 (fig 1) and in western blot on mouse samples at 1:2000 (fig s3). Nat Commun (2016) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D1D8)
  • immunocytochemistry knockout validation; mouse; loading ...; fig 2d
  • western blot knockout validation; mouse; 1:750; loading ...; fig 2a
Cell Signaling Technology Nf2 antibody (CST, 6995S) was used in immunocytochemistry knockout validation on mouse samples (fig 2d) and in western blot knockout validation on mouse samples at 1:750 (fig 2a). elife (2020) ncbi
Santa Cruz Biotechnology
mouse monoclonal (E-2)
  • western blot; human; loading ...; fig s1b
Santa Cruz Biotechnology Nf2 antibody (Santa Cruz, sc-55574) was used in western blot on human samples (fig s1b). Sci Rep (2017) ncbi
mouse monoclonal (B-12)
  • western blot; human; loading ...; fig s2
In order to explore how C-kinase potentiated protein phosphatase-1 inhibitor of 17 kDa drives Ras activity and tumorigenesis, Santa Cruz Biotechnology Nf2 antibody (Santa Cruz, B-12) was used in western blot on human samples (fig s2). Oncotarget (2016) ncbi
mouse monoclonal
  • western blot; human; loading ...; fig s2
In order to explore how C-kinase potentiated protein phosphatase-1 inhibitor of 17 kDa drives Ras activity and tumorigenesis, Santa Cruz Biotechnology Nf2 antibody (Santa Cruz, B-12) was used in western blot on human samples (fig s2). Oncotarget (2016) ncbi
mouse monoclonal (B-12)
  • western blot; human
Santa Cruz Biotechnology Nf2 antibody (Santa Cruz Biotechnology, sc-55575) was used in western blot on human samples . Brain (2013) ncbi
Abcam
mouse monoclonal (AF1G4)
  • western blot; human; 1:1000; loading ...; fig s2a
Abcam Nf2 antibody (Abcam, ab88957) was used in western blot on human samples at 1:1000 (fig s2a). Nat Commun (2016) ncbi
domestic rabbit monoclonal (EPR2573(2))
  • western blot knockout validation; human; 1:1000; fig 4
Abcam Nf2 antibody (Abcam, ab109244) was used in western blot knockout validation on human samples at 1:1000 (fig 4). Science (2014) ncbi
MilliporeSigma
domestic rabbit polyclonal
  • immunohistochemistry knockout validation; mouse; 1:300; fig 1
  • western blot; mouse; 1:2000; fig s3
In order to investigate the roles of NF2, LATS1/2, and YAP in the branching morphogenesis of the mouse kidney, MilliporeSigma Nf2 antibody (Sigma Prestige Antibodies, HPA003097) was used in immunohistochemistry knockout validation on mouse samples at 1:300 (fig 1) and in western blot on mouse samples at 1:2000 (fig s3). Nat Commun (2016) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D1D8)
  • immunocytochemistry knockout validation; mouse; loading ...; fig 2d
  • western blot knockout validation; mouse; 1:750; loading ...; fig 2a
Cell Signaling Technology Nf2 antibody (CST, 6995S) was used in immunocytochemistry knockout validation on mouse samples (fig 2d) and in western blot knockout validation on mouse samples at 1:750 (fig 2a). elife (2020) ncbi
domestic rabbit monoclonal (D1D8)
  • western blot; human; 1:1000; loading ...; fig st2
Cell Signaling Technology Nf2 antibody (Cell signalling, 6995) was used in western blot on human samples at 1:1000 (fig st2). Int J Mol Sci (2020) ncbi
domestic rabbit monoclonal (D1D8)
  • immunocytochemistry; human; loading ...; fig 4i
Cell Signaling Technology Nf2 antibody (Cell Signaling, 6995S) was used in immunocytochemistry on human samples (fig 4i). Curr Biol (2019) ncbi
domestic rabbit monoclonal (D1D8)
  • western blot; human; fig 1
Cell Signaling Technology Nf2 antibody (Cell signaling, 6995) was used in western blot on human samples (fig 1). J Biol Chem (2016) ncbi
domestic rabbit monoclonal (D1D8)
  • western blot; human; fig 2
Cell Signaling Technology Nf2 antibody (Cell Signaling, D1D8) was used in western blot on human samples (fig 2). Cell Death Differ (2016) ncbi
domestic rabbit monoclonal (D1D8)
  • western blot; mouse; 1:1000; fig 3
Cell Signaling Technology Nf2 antibody (Cell Signaling Tech, 6995) was used in western blot on mouse samples at 1:1000 (fig 3). PLoS ONE (2015) ncbi
domestic rabbit monoclonal (D1D8)
  • western blot; mouse; 1:1000; fig s1
Cell Signaling Technology Nf2 antibody (Cell Signaling, 6995) was used in western blot on mouse samples at 1:1000 (fig s1). Cell (2015) ncbi
domestic rabbit monoclonal (D1D8)
  • western blot; human; fig 3
Cell Signaling Technology Nf2 antibody (Cell Signaling Technology, 6995) was used in western blot on human samples (fig 3). PLoS ONE (2014) ncbi
domestic rabbit monoclonal (D1D8)
  • western blot; human; loading ...; fig 1a
Cell Signaling Technology Nf2 antibody (Cell Signaling Technology, 6995) was used in western blot on human samples (fig 1a). Oncogene (2015) ncbi
Articles Reviewed
  1. Fomicheva M, Macara I. Genome-wide CRISPR screen identifies noncanonical NF-κB signaling as a regulator of density-dependent proliferation. elife. 2020;9: pubmed publisher
  2. Adams C, Ercolano E, Ferluga S, Sofela A, Dave F, Negroni C, et al. A Rapid Robust Method for Subgrouping Non-NF2 Meningiomas According to Genotype and Detection of Lower Levels of M2 Macrophages in AKT1 E17K Mutated Tumours. Int J Mol Sci. 2020;21: pubmed publisher
  3. Rausch V, Bostrom J, Park J, Bravo I, Feng Y, Hay D, et al. The Hippo Pathway Regulates Caveolae Expression and Mediates Flow Response via Caveolae. Curr Biol. 2019;29:242-255.e6 pubmed publisher
  4. Das S, Jackson W, Prasain J, Hanna A, Bailey S, Tucker J, et al. Loss of Merlin induces metabolomic adaptation that engages dependence on Hedgehog signaling. Sci Rep. 2017;7:40773 pubmed publisher
  5. Riecken L, Zoch A, Wiehl U, Reichert S, Scholl I, Cui Y, et al. CPI-17 drives oncogenic Ras signaling in human melanomas via Ezrin-Radixin-Moesin family proteins. Oncotarget. 2016;7:78242-78254 pubmed publisher
  6. Chen Y, Xu J, Skanderup A, Dong Y, Brannon A, Wang L, et al. Molecular analysis of aggressive renal cell carcinoma with unclassified histology reveals distinct subsets. Nat Commun. 2016;7:13131 pubmed publisher
  7. Reginensi A, Enderle L, Gregorieff A, Johnson R, Wrana J, McNeill H. A critical role for NF2 and the Hippo pathway in branching morphogenesis. Nat Commun. 2016;7:12309 pubmed publisher
  8. Chen X, Stauffer S, Chen Y, Dong J. Ajuba Phosphorylation by CDK1 Promotes Cell Proliferation and Tumorigenesis. J Biol Chem. 2016;291:14761-72 pubmed publisher
  9. Chernova T, Sun X, Powley I, Galavotti S, Grosso S, Murphy F, et al. Molecular profiling reveals primary mesothelioma cell lines recapitulate human disease. Cell Death Differ. 2016;23:1152-64 pubmed publisher
  10. Zoch A, Mayerl S, Schulz A, Greither T, Frappart L, Rübsam J, et al. Merlin Isoforms 1 and 2 Both Act as Tumour Suppressors and Are Required for Optimal Sperm Maturation. PLoS ONE. 2015;10:e0129151 pubmed publisher
  11. Chen S, Sanjana N, Zheng K, Shalem O, Lee K, Shi X, et al. Genome-wide CRISPR screen in a mouse model of tumor growth and metastasis. Cell. 2015;160:1246-60 pubmed publisher
  12. Moriarity B, Rahrmann E, Beckmann D, Conboy C, Watson A, Carlson D, et al. Simple and efficient methods for enrichment and isolation of endonuclease modified cells. PLoS ONE. 2014;9:e96114 pubmed publisher
  13. Shalem O, Sanjana N, Hartenian E, Shi X, Scott D, Mikkelson T, et al. Genome-scale CRISPR-Cas9 knockout screening in human cells. Science. 2014;343:84-87 pubmed publisher
  14. Tanaka I, Osada H, Fujii M, Fukatsu A, Hida T, Horio Y, et al. LIM-domain protein AJUBA suppresses malignant mesothelioma cell proliferation via Hippo signaling cascade. Oncogene. 2015;34:73-83 pubmed publisher
  15. Doddrell R, Dun X, Shivane A, Feltri M, Wrabetz L, Wegner M, et al. Loss of SOX10 function contributes to the phenotype of human Merlin-null schwannoma cells. Brain. 2013;136:549-63 pubmed publisher