This is a Validated Antibody Database (VAD) review about rat Ncl, based on 32 published articles (read how Labome selects the articles), using Ncl antibody in all methods. It is aimed to help Labome visitors find the most suited Ncl antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Ncl synonym: nucleolin; nucleolin-like protein

Santa Cruz Biotechnology
mouse monoclonal (MS-3)
  • immunocytochemistry; human; 1:100; loading ...; fig 2h
Santa Cruz Biotechnology Ncl antibody (SantaCruz, sc-8031) was used in immunocytochemistry on human samples at 1:100 (fig 2h). Proc Natl Acad Sci U S A (2017) ncbi
mouse monoclonal (MS-3)
  • western blot; human; loading ...; fig 3a
Santa Cruz Biotechnology Ncl antibody (SantaCruz, sc-8031) was used in western blot on human samples (fig 3a). Open Biol (2016) ncbi
mouse monoclonal (MS-3)
  • western blot; human; loading ...; fig 1a
Santa Cruz Biotechnology Ncl antibody (Santa Cruz, SC-8031) was used in western blot on human samples (fig 1a). Oncotarget (2016) ncbi
mouse monoclonal (MS-3)
  • western blot; human; fig 4
Santa Cruz Biotechnology Ncl antibody (Santa Cruz, sc-8031) was used in western blot on human samples (fig 4). Sci Rep (2016) ncbi
mouse monoclonal (MS-3)
  • western blot; human; fig 3
Santa Cruz Biotechnology Ncl antibody (Santa Cruz, sc-8031) was used in western blot on human samples (fig 3). IUBMB Life (2016) ncbi
mouse monoclonal (MS-3)
  • immunoprecipitation; human; fig S5
Santa Cruz Biotechnology Ncl antibody (Santacruz, sc-8031) was used in immunoprecipitation on human samples (fig S5). Oncotarget (2016) ncbi
mouse monoclonal (MS-3)
  • western blot; human; fig 2
Santa Cruz Biotechnology Ncl antibody (Santa Cruz, sc-8031) was used in western blot on human samples (fig 2). Oncogene (2016) ncbi
mouse monoclonal (MS-3)
  • western blot; human
Santa Cruz Biotechnology Ncl antibody (Santa Cruz, sc-8031) was used in western blot on human samples . Neurobiol Aging (2015) ncbi
mouse monoclonal
  • western blot; human; loading ...; fig 2a
Santa Cruz Biotechnology Ncl antibody (Santa Cruz, sc-515312) was used in western blot on human samples (fig 2a). Toxicol Lett (2015) ncbi
mouse monoclonal (MS-3)
  • western blot; human; 1:1000
In order to study the role of Tn expression and post-translationally modifications in pancreatic ductal adenocarcinoma, Santa Cruz Biotechnology Ncl antibody (Santa Cruz, sc-8031) was used in western blot on human samples at 1:1000. Mol Cancer (2015) ncbi
mouse monoclonal (MS-3)
  • western blot; human; 1:1000; fig 3
Santa Cruz Biotechnology Ncl antibody (Santa Cruz, sc8031) was used in western blot on human samples at 1:1000 (fig 3). J Mol Biol (2015) ncbi
mouse monoclonal (MS-3)
  • immunoprecipitation; human; fig 1
  • western blot; human; fig 1
In order to characterize a potential treatment for glioblastoma by interfering with Erb1, Ras and nucleolin interactions, Santa Cruz Biotechnology Ncl antibody (Santa Cruz, sc-8031) was used in immunoprecipitation on human samples (fig 1) and in western blot on human samples (fig 1). Oncotarget (2014) ncbi
mouse monoclonal (MS-3)
  • immunocytochemistry; mouse
Santa Cruz Biotechnology Ncl antibody (Santa Cruz, sc-8031) was used in immunocytochemistry on mouse samples . Stem Cells Dev (2014) ncbi
mouse monoclonal (MS-3)
  • immunoprecipitation; human; fig s1c
Santa Cruz Biotechnology Ncl antibody (Santa, sc-8031) was used in immunoprecipitation on human samples (fig s1c). Nucleic Acids Res (2014) ncbi
Abcam
rabbit polyclonal
  • immunocytochemistry; human; loading ...; fig 4a
  • western blot; human; loading ...; fig 4b
Abcam Ncl antibody (Abcam, ab22758) was used in immunocytochemistry on human samples (fig 4a) and in western blot on human samples (fig 4b). J Am Chem Soc (2019) ncbi
rabbit polyclonal
  • immunoprecipitation; human; loading ...; fig 6a
Abcam Ncl antibody (Abcam, ab22758) was used in immunoprecipitation on human samples (fig 6a). Proc Natl Acad Sci U S A (2018) ncbi
rabbit polyclonal
  • western blot; human; loading ...; fig 1c
In order to report the interactome of all dipeptide repeat proteins, Abcam Ncl antibody (Abcam, ab22758) was used in western blot on human samples (fig 1c). Cell (2016) ncbi
rabbit polyclonal
  • immunocytochemistry; human; fig 1
In order to study how PARylation regulates Top1 nuclear dynamics, Abcam Ncl antibody (Abcam, ab22758) was used in immunocytochemistry on human samples (fig 1). Nucleic Acids Res (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:1000; fig 6a
  • western blot; human; 1:100; fig 7a
In order to examine the composition of mitochondrial proteins in cases of fatal familial insomnia, Abcam Ncl antibody (Abcam, Ab22758) was used in immunohistochemistry - paraffin section on human samples at 1:1000 (fig 6a) and in western blot on human samples at 1:100 (fig 7a). Brain Pathol (2017) ncbi
rabbit polyclonal
  • immunocytochemistry; human; fig 2
In order to elucidate how genome integrity is protected from R-loops in the fanconi anemia pathway, Abcam Ncl antibody (Abcam, ab50279) was used in immunocytochemistry on human samples (fig 2). PLoS Genet (2015) ncbi
rabbit polyclonal
  • immunocytochemistry; rat; 1:100; fig 3
In order to discuss intracellular functions of versican, Abcam Ncl antibody (Abcam, ab22758) was used in immunocytochemistry on rat samples at 1:100 (fig 3). Cardiovasc Pathol (2015) ncbi
rabbit polyclonal
  • western blot; human; 1:3000; fig 2
Abcam Ncl antibody (Abcam, 22758) was used in western blot on human samples at 1:3000 (fig 2). Front Microbiol (2015) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000; fig  6
Abcam Ncl antibody (Abcam, Ab22758) was used in western blot on mouse samples at 1:1000 (fig  6). J Mol Cell Cardiol (2015) ncbi
rabbit polyclonal
  • western blot; human
Abcam Ncl antibody (Abcam, ab22758) was used in western blot on human samples . J Biol Chem (2015) ncbi
rabbit polyclonal
  • immunocytochemistry; human; fig 6
Abcam Ncl antibody (Abcam, ab22758) was used in immunocytochemistry on human samples (fig 6). Cancer Sci (2015) ncbi
rabbit polyclonal
  • immunocytochemistry; human; fig s5
Abcam Ncl antibody (Abcam, ab22758) was used in immunocytochemistry on human samples (fig s5). Nucleus (2014) ncbi
rabbit polyclonal
  • immunocytochemistry; mouse; fig 5
In order to study why HSC function declines with age, Abcam Ncl antibody (Abcam, ab22758) was used in immunocytochemistry on mouse samples (fig 5). Nature (2014) ncbi
rabbit polyclonal
  • immunocytochemistry; human
  • western blot; human
In order to investigate the role of nuclear ARVCF protein during alternative splicing, Abcam Ncl antibody (Abcam, ab22758) was used in immunocytochemistry on human samples and in western blot on human samples . J Biol Chem (2014) ncbi
rabbit polyclonal
  • western blot; human
Abcam Ncl antibody (Abcam, ab50279) was used in western blot on human samples . Nucleic Acids Res (2013) ncbi
Proteintech Group
rabbit polyclonal
  • immunohistochemistry - paraffin section; human; loading ...; fig 3
Proteintech Group Ncl antibody (Proteintech, 10556-1-AP) was used in immunohistochemistry - paraffin section on human samples (fig 3). Acta Neuropathol (2015) ncbi
Abnova
rabbit polyclonal
  • western blot; human; fig 1
Abnova Ncl antibody (Abnova, PAB12541) was used in western blot on human samples (fig 1). Mol Cell Proteomics (2015) ncbi
Novus Biologicals
rabbit polyclonal
  • western blot; human; 1:4000; fig 4c
Novus Biologicals Ncl antibody (Novus, NB600-241) was used in western blot on human samples at 1:4000 (fig 4c). Sci Rep (2016) ncbi
Articles Reviewed
  1. Wang F, Zhou S, Qi D, Xiang S, Wong E, Wang X, et al. Nucleolin Is a Functional Binding Protein for Salinomycin in Neuroblastoma Stem Cells. J Am Chem Soc. 2019;141:3613-3622 pubmed publisher
  2. Castanotto D, Zhang X, Alluin J, Zhang X, Rüger J, Armstrong B, et al. A stress-induced response complex (SIRC) shuttles miRNAs, siRNAs, and oligonucleotides to the nucleus. Proc Natl Acad Sci U S A. 2018;115:E5756-E5765 pubmed publisher
  3. Coccia M, Rossi A, Riccio A, Trotta E, Santoro M. Human NF-κB repressing factor acts as a stress-regulated switch for ribosomal RNA processing and nucleolar homeostasis surveillance. Proc Natl Acad Sci U S A. 2017;114:1045-1050 pubmed publisher
  4. Samson A, Ho B, Au A, Schoenwaelder S, Smyth M, Bottomley S, et al. Physicochemical properties that control protein aggregation also determine whether a protein is retained or released from necrotic cells. Open Biol. 2016;6: pubmed
  5. Lee K, Zhang P, Kim H, Mitrea D, Sarkar M, Freibaum B, et al. C9orf72 Dipeptide Repeats Impair the Assembly, Dynamics, and Function of Membrane-Less Organelles. Cell. 2016;167:774-788.e17 pubmed publisher
  6. Das S, Rehman I, Ghosh A, Sengupta S, Majumdar P, Jana B, et al. Poly(ADP-ribose) polymers regulate DNA topoisomerase I (Top1) nuclear dynamics and camptothecin sensitivity in living cells. Nucleic Acids Res. 2016;44:8363-75 pubmed publisher
  7. Robijns J, Molenberghs F, Sieprath T, Corne T, Verschuuren M, De Vos W. In silico synchronization reveals regulators of nuclear ruptures in lamin A/C deficient model cells. Sci Rep. 2016;6:30325 pubmed publisher
  8. Kim Y, Lee M, Kim H, Kim J. Ribosomal protein S3 (rpS3) secreted from various cancer cells is N-linked glycosylated. Oncotarget. 2016;7:80350-80362 pubmed publisher
  9. Terrier O, Carron C, de Chassey B, Dubois J, Traversier A, Julien T, et al. Nucleolin interacts with influenza A nucleoprotein and contributes to viral ribonucleoprotein complexes nuclear trafficking and efficient influenza viral replication. Sci Rep. 2016;6:29006 pubmed publisher
  10. Frau Méndez M, Fernández Vega I, Ansoleaga B, Blanco Tech R, Carmona Tech M, Antonio Del Río J, et al. Fatal familial insomnia: mitochondrial and protein synthesis machinery decline in the mediodorsal thalamus. Brain Pathol. 2017;27:95-106 pubmed publisher
  11. Bober J, Olsnes S, Kostas M, Bogacz M, Zakrzewska M, Otlewski J. Identification of new FGF1 binding partners-Implications for its intracellular function. IUBMB Life. 2016;68:242-51 pubmed publisher
  12. Nogales V, Reinhold W, Varma S, Martinez Cardus A, Moutinho C, Moran S, et al. Epigenetic inactivation of the putative DNA/RNA helicase SLFN11 in human cancer confers resistance to platinum drugs. Oncotarget. 2016;7:3084-97 pubmed publisher
  13. García Rubio M, Pérez Calero C, Barroso S, Tumini E, Herrera Moyano E, Rosado I, et al. The Fanconi Anemia Pathway Protects Genome Integrity from R-loops. PLoS Genet. 2015;11:e1005674 pubmed publisher
  14. Felli N, Errico M, Pedini F, Petrini M, Puglisi R, Bellenghi M, et al. AP2α controls the dynamic balance between miR-126&126* and miR-221&222 during melanoma progression. Oncogene. 2016;35:3016-26 pubmed publisher
  15. Carthy J, Abraham T, Meredith A, Boroomand S, McManus B. Versican localizes to the nucleus in proliferating mesenchymal cells. Cardiovasc Pathol. 2015;24:368-74 pubmed publisher
  16. Walker E, Jensen L, Croft S, Ghildyal R. Variation in the nuclear effects of infection by different human rhinovirus serotypes. Front Microbiol. 2015;6:875 pubmed publisher
  17. Li M, Pehar M, Liu Y, Bhattacharyya A, Zhang S, O Riordan K, et al. The amyloid precursor protein (APP) intracellular domain regulates translation of p44, a short isoform of p53, through an IRES-dependent mechanism. Neurobiol Aging. 2015;36:2725-36 pubmed publisher
  18. Chiou Y, Liou S, Wong R, Chen C, Lee H. Nickel may contribute to EGFR mutation and synergistically promotes tumor invasion in EGFR-mutated lung cancer via nickel-induced microRNA-21 expression. Toxicol Lett. 2015;237:46-54 pubmed publisher
  19. Hofmann B, Schlüter L, Lange P, Mercanoglu B, Ewald F, Fölster A, et al. COSMC knockdown mediated aberrant O-glycosylation promotes oncogenic properties in pancreatic cancer. Mol Cancer. 2015;14:109 pubmed publisher
  20. Revuelta López E, Cal R, Herraiz Martínez A, De Gonzalo Calvo D, Nasarre L, Roura S, et al. Hypoxia-driven sarcoplasmic/endoplasmic reticulum calcium ATPase 2 (SERCA2) downregulation depends on low-density lipoprotein receptor-related protein 1 (LRP1)-signalling in cardiomyocytes. J Mol Cell Cardiol. 2015;85:25-36 pubmed publisher
  21. Cooper Knock J, Higginbottom A, Stopford M, Highley J, Ince P, Wharton S, et al. Antisense RNA foci in the motor neurons of C9ORF72-ALS patients are associated with TDP-43 proteinopathy. Acta Neuropathol. 2015;130:63-75 pubmed publisher
  22. Gago Fuentes R, Fernández Puente P, Megias D, Carpintero Fernández P, Mateos J, Acea B, et al. Proteomic Analysis of Connexin 43 Reveals Novel Interactors Related to Osteoarthritis. Mol Cell Proteomics. 2015;14:1831-45 pubmed publisher
  23. Mayor R, Izquierdo Bouldstridge A, Millán Ariño L, Bustillos A, Sampaio C, Luque N, et al. Genome distribution of replication-independent histone H1 variants shows H1.0 associated with nucleolar domains and H1X associated with RNA polymerase II-enriched regions. J Biol Chem. 2015;290:7474-91 pubmed publisher
  24. Szerlong H, Herman J, Krause C, DeLuca J, Skoultchi A, Winger Q, et al. Proteomic characterization of the nucleolar linker histone H1 interaction network. J Mol Biol. 2015;427:2056-71 pubmed publisher
  25. Nakaguro M, Kiyonari S, Kishida S, Cao D, Murakami Tonami Y, Ichikawa H, et al. Nucleolar protein PES1 is a marker of neuroblastoma outcome and is associated with neuroblastoma differentiation. Cancer Sci. 2015;106:237-43 pubmed publisher
  26. Ragoczy T, Telling A, Scalzo D, Kooperberg C, Groudine M. Functional redundancy in the nuclear compartmentalization of the late-replicating genome. Nucleus. 2014;5:626-35 pubmed publisher
  27. Goldshmit Y, Trangle S, Kloog Y, Pinkas Kramarski R. Interfering with the interaction between ErbB1, nucleolin and Ras as a potential treatment for glioblastoma. Oncotarget. 2014;5:8602-13 pubmed
  28. Flach J, Bakker S, Mohrin M, Conroy P, Pietras E, Reynaud D, et al. Replication stress is a potent driver of functional decline in ageing haematopoietic stem cells. Nature. 2014;512:198-202 pubmed publisher
  29. Lührig S, Siamishi I, Tesmer Wolf M, Zechner U, Engel W, Nolte J. Lrrc34, a novel nucleolar protein, interacts with npm1 and ncl and has an impact on pluripotent stem cells. Stem Cells Dev. 2014;23:2862-74 pubmed publisher
  30. Ullius A, Lüscher Firzlaff J, Costa I, Walsemann G, Forst A, Gusmao E, et al. The interaction of MYC with the trithorax protein ASH2L promotes gene transcription by regulating H3K27 modification. Nucleic Acids Res. 2014;42:6901-20 pubmed publisher
  31. Rappe U, Schlechter T, Aschoff M, Hotz Wagenblatt A, Hofmann I. Nuclear ARVCF protein binds splicing factors and contributes to the regulation of alternative splicing. J Biol Chem. 2014;289:12421-34 pubmed publisher
  32. Peddigari S, Li P, Rabe J, Martin S. hnRNPL and nucleolin bind LINE-1 RNA and function as host factors to modulate retrotransposition. Nucleic Acids Res. 2013;41:575-85 pubmed publisher