This is a Validated Antibody Database (VAD) review about rat Myh9, based on 16 published articles (read how Labome selects the articles), using Myh9 antibody in all methods. It is aimed to help Labome visitors find the most suited Myh9 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Myh9 synonym: NMMHC II-a; NMMHC-A; NMMHC-IIA

BioLegend
domestic rabbit polyclonal (Poly19098)
  • western blot; human; loading ...; fig 7b
BioLegend Myh9 antibody (Biolegend, 909801) was used in western blot on human samples (fig 7b). Cells (2022) ncbi
domestic rabbit polyclonal (Poly19098)
  • immunocytochemistry; mouse; 1:800; loading ...; fig 1a
  • immunocytochemistry; human; 1:800; loading ...; fig 1a
BioLegend Myh9 antibody (Biolegend, 909801) was used in immunocytochemistry on mouse samples at 1:800 (fig 1a) and in immunocytochemistry on human samples at 1:800 (fig 1a). elife (2021) ncbi
domestic rabbit polyclonal (Poly19098)
  • immunocytochemistry; human; loading ...; fig 3g
BioLegend Myh9 antibody (BioLegend, 909801) was used in immunocytochemistry on human samples (fig 3g). Curr Biol (2019) ncbi
domestic rabbit polyclonal (Poly19098)
  • immunocytochemistry; human; 1:200; loading ...; fig s1a
BioLegend Myh9 antibody (BioLegend, 909801) was used in immunocytochemistry on human samples at 1:200 (fig s1a). Sci Rep (2018) ncbi
domestic rabbit polyclonal (Poly19098)
  • immunocytochemistry; mouse; loading ...; fig s5j
BioLegend Myh9 antibody (Covance, PRB-440P) was used in immunocytochemistry on mouse samples (fig s5j). Dev Cell (2018) ncbi
domestic rabbit polyclonal (Poly19098)
  • immunocytochemistry; human; 1:500; loading ...; fig 5d, 7b
  • western blot; human; 1:1000; loading ...; fig 5b
BioLegend Myh9 antibody (Covance, PRB-440P) was used in immunocytochemistry on human samples at 1:500 (fig 5d, 7b) and in western blot on human samples at 1:1000 (fig 5b). Nat Commun (2018) ncbi
domestic rabbit polyclonal (Poly19098)
  • western blot; human; 1:1000; loading ...; fig 6c
BioLegend Myh9 antibody (Covance, PRB-440P) was used in western blot on human samples at 1:1000 (fig 6c). PLoS ONE (2018) ncbi
domestic rabbit polyclonal (Poly19098)
  • immunocytochemistry; dogs; loading ...
BioLegend Myh9 antibody (BioLegend, 909801) was used in immunocytochemistry on dogs samples . PLoS ONE (2017) ncbi
domestic rabbit polyclonal (Poly19098)
  • immunocytochemistry; human; loading ...; fig s8a
BioLegend Myh9 antibody (Covance, PRB-440P) was used in immunocytochemistry on human samples (fig s8a). Mol Biol Cell (2017) ncbi
domestic rabbit polyclonal (Poly19098)
  • western blot; human; loading ...; fig s4a
BioLegend Myh9 antibody (Covance, PRB-440P) was used in western blot on human samples (fig s4a). Oncotarget (2017) ncbi
domestic rabbit polyclonal (Poly19098)
  • western blot; human; 1:1000; loading ...; fig 5c
In order to analyze differential functional effects for rho kinases 1 and 2 at the epithelial zonula adherens, BioLegend Myh9 antibody (Covance, PRB-440P) was used in western blot on human samples at 1:1000 (fig 5c). Mol Biol Cell (2017) ncbi
domestic rabbit polyclonal (Poly19098)
  • immunocytochemistry; human; 1:1000; loading ...; fig 1d
  • western blot; human; loading ...; fig 1f
In order to investigate the role of Coronin 1B in regulating the non-muscle myosin II pool, BioLegend Myh9 antibody (Covance, PRB-440P) was used in immunocytochemistry on human samples at 1:1000 (fig 1d) and in western blot on human samples (fig 1f). Cell Cycle (2016) ncbi
domestic rabbit polyclonal (Poly19098)
  • immunocytochemistry; human; loading ...; fig 1a
In order to find that mitotic focal adhesions link mitotic cell shape and spindle orientation, BioLegend Myh9 antibody (BioLegends, 909801) was used in immunocytochemistry on human samples (fig 1a). Sci Rep (2016) ncbi
domestic rabbit polyclonal (Poly19098)
  • immunohistochemistry; zebrafish ; 1:100; fig 4
In order to assess the drive of lumen formation via blood flow through inverse membrane blebbing during angiogenesis in vivo, BioLegend Myh9 antibody (Covance, PRB-440P) was used in immunohistochemistry on zebrafish samples at 1:100 (fig 4). Nat Cell Biol (2016) ncbi
Abcam
domestic rabbit polyclonal
  • western blot; pigs ; 1:1000; loading ...; fig 2a
Abcam Myh9 antibody (Abcam, ab75590) was used in western blot on pigs samples at 1:1000 (fig 2a). BMC Genomics (2019) ncbi
Invitrogen
domestic rabbit polyclonal
In order to investigate how the cortical actin meshwork in the rear develops, Invitrogen Myh9 antibody (Thermo Scientific, PA5-17025) was used . Nat Commun (2015) ncbi
Articles Reviewed
  1. Naydenov N, Lechuga S, Zalavadia A, Mukherjee P, Gordon I, Skvasik D, et al. P-Cadherin Regulates Intestinal Epithelial Cell Migration and Mucosal Repair, but Is Dispensable for Colitis Associated Colon Cancer. Cells. 2022;11: pubmed publisher
  2. Lehtimäki J, Rajakylä E, Tojkander S, Lappalainen P. Generation of stress fibers through myosin-driven reorganization of the actin cortex. elife. 2021;10: pubmed publisher
  3. Zhao Y, Lu X, Cheng Z, Tian M, Qiangba Y, Fu Q, et al. Comparative proteomic analysis of Tibetan pig spermatozoa at high and low altitudes. BMC Genomics. 2019;20:569 pubmed publisher
  4. Jiu Y, Kumari R, Fenix A, Schaible N, Liu X, Varjosalo M, et al. Myosin-18B Promotes the Assembly of Myosin II Stacks for Maturation of Contractile Actomyosin Bundles. Curr Biol. 2019;29:81-92.e5 pubmed publisher
  5. Ciuba K, Hawkes W, Tojkander S, Kogan K, Engel U, Iskratsch T, et al. Calponin-3 is critical for coordinated contractility of actin stress fibers. Sci Rep. 2018;8:17670 pubmed publisher
  6. Schell C, Sabass B, Helmstaedter M, Geist F, Abed A, Yasuda Yamahara M, et al. ARP3 Controls the Podocyte Architecture at the Kidney Filtration Barrier. Dev Cell. 2018;47:741-757.e8 pubmed publisher
  7. Latham S, Ehmke N, Reinke P, Taft M, Eicke D, Reindl T, et al. Variants in exons 5 and 6 of ACTB cause syndromic thrombocytopenia. Nat Commun. 2018;9:4250 pubmed publisher
  8. Yasuda Yamahara M, Rogg M, Yamahara K, Maier J, Huber T, Schell C. AIF1L regulates actomyosin contractility and filopodial extensions in human podocytes. PLoS ONE. 2018;13:e0200487 pubmed publisher
  9. Fredriksson Lidman K, Van Itallie C, Tietgens A, Anderson J. Sorbin and SH3 domain-containing protein 2 (SORBS2) is a component of the acto-myosin ring at the apical junctional complex in epithelial cells. PLoS ONE. 2017;12:e0185448 pubmed publisher
  10. Owen L, Adhikari A, Patel M, Grimmer P, Leijnse N, Kim M, et al. A cytoskeletal clutch mediates cellular force transmission in a soft, three-dimensional extracellular matrix. Mol Biol Cell. 2017;28:1959-1974 pubmed publisher
  11. Jeong S, Lim S, Schevzov G, Gunning P, Helfman D. Loss of Tpm4.1 leads to disruption of cell-cell adhesions and invasive behavior in breast epithelial cells via increased Rac1 signaling. Oncotarget. 2017;8:33544-33559 pubmed publisher
  12. Priya R, Liang X, Teo J, Duszyc K, Yap A, Gomez G. ROCK1 but not ROCK2 contributes to RhoA signaling and NMIIA-mediated contractility at the epithelial zonula adherens. Mol Biol Cell. 2017;28:12-20 pubmed publisher
  13. Priya R, Wee K, Budnar S, Gomez G, Yap A, Michael M. Coronin 1B supports RhoA signaling at cell-cell junctions through Myosin II. Cell Cycle. 2016;15:3033-3041 pubmed
  14. Taneja N, Fenix A, Rathbun L, Millis B, Tyska M, Hehnly H, et al. Focal adhesions control cleavage furrow shape and spindle tilt during mitosis. Sci Rep. 2016;6:29846 pubmed publisher
  15. Gebala V, Collins R, Geudens I, Phng L, Gerhardt H. Blood flow drives lumen formation by inverse membrane blebbing during angiogenesis in vivo. Nat Cell Biol. 2016;18:443-50 pubmed publisher
  16. Ramalingam N, Franke C, Jaschinski E, Winterhoff M, Lu Y, Brühmann S, et al. A resilient formin-derived cortical actin meshwork in the rear drives actomyosin-based motility in 2D confinement. Nat Commun. 2015;6:8496 pubmed publisher