This is a Validated Antibody Database (VAD) review about rat Mecp2, based on 29 published articles (read how Labome selects the articles), using Mecp2 antibody in all methods. It is aimed to help Labome visitors find the most suited Mecp2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Knockout validation
Invitrogen
domestic rabbit polyclonal
  • western blot knockout validation; mouse; 1:1000; loading ...; fig s6
In order to elucidate the regulation of Sapap3 and show essential roles for striatal Hdac1, Hdac2, and MeCP2 in repetitive behavior suppression, Invitrogen Mecp2 antibody (ThermoFisher, PA1-888) was used in western blot knockout validation on mouse samples at 1:1000 (fig s6). Nat Neurosci (2016) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D4F3)
  • western blot knockout validation; mouse; 1:500; loading ...; fig 1s1a
  • immunohistochemistry - frozen section; mouse; 1:500; loading ...; fig 1s1b
Cell Signaling Technology Mecp2 antibody (Cell Signaling, 3456) was used in western blot knockout validation on mouse samples at 1:500 (fig 1s1a) and in immunohistochemistry - frozen section on mouse samples at 1:500 (fig 1s1b). elife (2020) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D4F3)
  • western blot knockout validation; human; loading ...; fig 1e
  • immunocytochemistry; human; loading ...; fig 1h
In order to investigate the involvement of CREB signaling in the etiology of Rett syndrome, Cell Signaling Technology Mecp2 antibody (Cell Signaling, 3456S) was used in western blot knockout validation on human samples (fig 1e) and in immunocytochemistry on human samples (fig 1h). J Neurosci (2017) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D4F3)
  • immunohistochemistry knockout validation; mouse; 1:500; loading ...; fig 4g
In order to assess the efficacy of a self-complementary adeno-associated virus 9 vector expressing a codon-optimized version of the methyl CpG binding protein 2 gene, Cell Signaling Technology Mecp2 antibody (Cell Signaling, 3456) was used in immunohistochemistry knockout validation on mouse samples at 1:500 (fig 4g). Neurobiol Dis (2017) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D4F3)
  • immunohistochemistry knockout validation; mouse; fig 1
  • immunohistochemistry - free floating section; mouse; fig 1
Cell Signaling Technology Mecp2 antibody (Cell signaling, 3456S) was used in immunohistochemistry knockout validation on mouse samples (fig 1) and in immunohistochemistry - free floating section on mouse samples (fig 1). elife (2016) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D4F3)
  • immunohistochemistry knockout validation; mouse; fig 1
  • western blot knockout validation; mouse; fig 4
  • immunohistochemistry - frozen section; mouse; fig 1
Cell Signaling Technology Mecp2 antibody (Cell signaling, 3456) was used in immunohistochemistry knockout validation on mouse samples (fig 1), in western blot knockout validation on mouse samples (fig 4) and in immunohistochemistry - frozen section on mouse samples (fig 1). Genes Cells (2015) ncbi
Invitrogen
domestic rabbit polyclonal
  • western blot knockout validation; mouse; 1:1000; loading ...; fig s6
In order to elucidate the regulation of Sapap3 and show essential roles for striatal Hdac1, Hdac2, and MeCP2 in repetitive behavior suppression, Invitrogen Mecp2 antibody (ThermoFisher, PA1-888) was used in western blot knockout validation on mouse samples at 1:1000 (fig s6). Nat Neurosci (2016) ncbi
domestic rabbit polyclonal
  • western blot; fruit fly ; 1:5000; fig 2
In order to study the function of the MeCP2 C-terminus, Invitrogen Mecp2 antibody (Thermo Scientific, PA1-887) was used in western blot on fruit fly samples at 1:5000 (fig 2). PLoS ONE (2016) ncbi
Active Motif
mouse monoclonal (4B4)
  • ChIP-Seq; mouse; loading ...; fig 3k
Active Motif Mecp2 antibody (Active Motif, 61285) was used in ChIP-Seq on mouse samples (fig 3k). Epigenetics (2017) ncbi
mouse monoclonal (4B4)
  • immunohistochemistry; rat; 1:200
Active Motif Mecp2 antibody (Active Motif, 4B4) was used in immunohistochemistry on rat samples at 1:200. J Chem Neuroanat (2014) ncbi
Abcam
domestic rabbit polyclonal
  • western blot; human; fig 3a
Abcam Mecp2 antibody (Abcam, ab2828) was used in western blot on human samples (fig 3a). Cell Death Dis (2021) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D4F3)
  • immunohistochemistry - frozen section; mouse; 1:150; loading ...; fig 1b
  • western blot; mouse; 1:2000; loading ...; fig 6a
Cell Signaling Technology Mecp2 antibody (Cell Signaling, 3456) was used in immunohistochemistry - frozen section on mouse samples at 1:150 (fig 1b) and in western blot on mouse samples at 1:2000 (fig 6a). Sci Rep (2021) ncbi
domestic rabbit monoclonal (D4F3)
  • immunohistochemistry - free floating section; mouse; 1:500; loading ...; fig 5a
Cell Signaling Technology Mecp2 antibody (Cell Signaling, 3456) was used in immunohistochemistry - free floating section on mouse samples at 1:500 (fig 5a). Sci Rep (2021) ncbi
domestic rabbit monoclonal (D4F3)
  • immunohistochemistry; mouse; 1:200; loading ...; fig 1a
Cell Signaling Technology Mecp2 antibody (Cell Signaling Technology, 3456 s) was used in immunohistochemistry on mouse samples at 1:200 (fig 1a). elife (2020) ncbi
domestic rabbit monoclonal (D4F3)
  • western blot knockout validation; mouse; 1:500; loading ...; fig 1s1a
  • immunohistochemistry - frozen section; mouse; 1:500; loading ...; fig 1s1b
Cell Signaling Technology Mecp2 antibody (Cell Signaling, 3456) was used in western blot knockout validation on mouse samples at 1:500 (fig 1s1a) and in immunohistochemistry - frozen section on mouse samples at 1:500 (fig 1s1b). elife (2020) ncbi
domestic rabbit monoclonal (D4F3)
  • western blot; mouse; 1:1000; loading ...; fig 3a
Cell Signaling Technology Mecp2 antibody (CST, 3456) was used in western blot on mouse samples at 1:1000 (fig 3a). Mol Ther Nucleic Acids (2018) ncbi
domestic rabbit monoclonal (D4F3)
  • chromatin immunoprecipitation; mouse; fig 6f
Cell Signaling Technology Mecp2 antibody (Cell Signaling, 3456) was used in chromatin immunoprecipitation on mouse samples (fig 6f). Neurobiol Dis (2018) ncbi
domestic rabbit monoclonal (D4F3)
  • western blot; mouse; loading ...; fig 4a
In order to assess the role of MECP2 in tau pathology using several mouse models, Cell Signaling Technology Mecp2 antibody (Cell Signaling, 3456) was used in western blot on mouse samples (fig 4a). Front Mol Neurosci (2017) ncbi
domestic rabbit monoclonal (D4F3)
  • western blot knockout validation; human; loading ...; fig 1e
  • immunocytochemistry; human; loading ...; fig 1h
In order to investigate the involvement of CREB signaling in the etiology of Rett syndrome, Cell Signaling Technology Mecp2 antibody (Cell Signaling, 3456S) was used in western blot knockout validation on human samples (fig 1e) and in immunocytochemistry on human samples (fig 1h). J Neurosci (2017) ncbi
domestic rabbit monoclonal (D4F3)
  • immunohistochemistry knockout validation; mouse; 1:500; loading ...; fig 4g
In order to assess the efficacy of a self-complementary adeno-associated virus 9 vector expressing a codon-optimized version of the methyl CpG binding protein 2 gene, Cell Signaling Technology Mecp2 antibody (Cell Signaling, 3456) was used in immunohistochemistry knockout validation on mouse samples at 1:500 (fig 4g). Neurobiol Dis (2017) ncbi
domestic rabbit monoclonal (D4F3)
  • immunohistochemistry knockout validation; mouse; fig 1
  • immunohistochemistry - free floating section; mouse; fig 1
Cell Signaling Technology Mecp2 antibody (Cell signaling, 3456S) was used in immunohistochemistry knockout validation on mouse samples (fig 1) and in immunohistochemistry - free floating section on mouse samples (fig 1). elife (2016) ncbi
domestic rabbit monoclonal (D4F3)
  • immunohistochemistry; mouse; 1:200
Cell Signaling Technology Mecp2 antibody (Cell Signaling, 3456S) was used in immunohistochemistry on mouse samples at 1:200. elife (2016) ncbi
domestic rabbit monoclonal (D4F3)
  • chromatin immunoprecipitation; human; loading ...; fig 4e
  • immunoprecipitation; human; loading ...; fig 4b
  • western blot; human; loading ...; fig 4a
Cell Signaling Technology Mecp2 antibody (Cell Signaling, 3456) was used in chromatin immunoprecipitation on human samples (fig 4e), in immunoprecipitation on human samples (fig 4b) and in western blot on human samples (fig 4a). Oncotarget (2016) ncbi
domestic rabbit monoclonal (D4F3)
  • immunoprecipitation; mouse; fig 2
  • western blot; mouse; 1:10,000; fig 2
Cell Signaling Technology Mecp2 antibody (Cell Signaling, 3456) was used in immunoprecipitation on mouse samples (fig 2) and in western blot on mouse samples at 1:10,000 (fig 2). Nat Commun (2016) ncbi
domestic rabbit monoclonal (D4F3)
  • immunocytochemistry; mouse; 1:500; fig 3
Cell Signaling Technology Mecp2 antibody (Cell signaling, 3456) was used in immunocytochemistry on mouse samples at 1:500 (fig 3). Nat Commun (2016) ncbi
domestic rabbit monoclonal (D4F3)
  • immunohistochemistry; African green monkey; fig 1
  • western blot; African green monkey; fig 1
In order to discuss using genetically engineered non-human primates to study brain disorders, Cell Signaling Technology Mecp2 antibody (Cell Signaling, 3456S) was used in immunohistochemistry on African green monkey samples (fig 1) and in western blot on African green monkey samples (fig 1). Nature (2016) ncbi
domestic rabbit monoclonal (D4F3)
  • immunohistochemistry knockout validation; mouse; fig 1
  • western blot knockout validation; mouse; fig 4
  • immunohistochemistry - frozen section; mouse; fig 1
Cell Signaling Technology Mecp2 antibody (Cell signaling, 3456) was used in immunohistochemistry knockout validation on mouse samples (fig 1), in western blot knockout validation on mouse samples (fig 4) and in immunohistochemistry - frozen section on mouse samples (fig 1). Genes Cells (2015) ncbi
domestic rabbit monoclonal (D4F3)
  • western blot; mouse; fig 1c
In order to describe how PTP1B is a mechanism-based therapeutic target for Rett syndrome, Cell Signaling Technology Mecp2 antibody (Cell Signaling Technology, 3456) was used in western blot on mouse samples (fig 1c). J Clin Invest (2015) ncbi
domestic rabbit monoclonal (D4F3)
  • western blot; mouse; 1:1000; fig 2
In order to study the muscular defects in Mecp2-null mice, Cell Signaling Technology Mecp2 antibody (Cell Signaling, 3456) was used in western blot on mouse samples at 1:1000 (fig 2). PLoS ONE (2015) ncbi
domestic rabbit monoclonal (D4F3)
  • immunohistochemistry; mouse; 1:200; fig 1
Cell Signaling Technology Mecp2 antibody (Cell Signaling, D4F3) was used in immunohistochemistry on mouse samples at 1:200 (fig 1). Cereb Cortex (2016) ncbi
domestic rabbit monoclonal (D4F3)
  • immunohistochemistry - frozen section; mouse; 1:500; fig 2
  • western blot; mouse; 1:1000; fig 2
Cell Signaling Technology Mecp2 antibody (CST, 3456) was used in immunohistochemistry - frozen section on mouse samples at 1:500 (fig 2) and in western blot on mouse samples at 1:1000 (fig 2). Mol Pain (2015) ncbi
domestic rabbit monoclonal (D4F3)
  • western blot; mouse; 1:1000
Cell Signaling Technology Mecp2 antibody (Cell Signaling Technology, 3456) was used in western blot on mouse samples at 1:1000. Neuroscience (2014) ncbi
domestic rabbit monoclonal (D4F3)
  • western blot; human
Cell Signaling Technology Mecp2 antibody (Cell Signaling, 3456) was used in western blot on human samples . PLoS ONE (2013) ncbi
domestic rabbit monoclonal (D4F3)
  • western blot; human; 1:1000
In order to describe the effect of SUMOlyation on methyl CpG binding protein 2 function, Cell Signaling Technology Mecp2 antibody (Cell Signaling Technology, #3456) was used in western blot on human samples at 1:1000. J Neurochem (2014) ncbi
domestic rabbit monoclonal (D4F3)
  • immunocytochemistry; human
  • western blot; human
Cell Signaling Technology Mecp2 antibody (Epitomics, 3456) was used in immunocytochemistry on human samples and in western blot on human samples . Proc Natl Acad Sci U S A (2012) ncbi
Articles Reviewed
  1. Villani C, Carli M, Castaldo A, Sacchetti G, Invernizzi R. Fluoxetine increases brain MeCP2 immuno-positive cells in a female Mecp2 heterozygous mouse model of Rett syndrome through endogenous serotonin. Sci Rep. 2021;11:14690 pubmed publisher
  2. Uchida K, Hasuoka K, Fuse T, Kobayashi K, Moriya T, Suzuki M, et al. Thyroid hormone insufficiency alters the expression of psychiatric disorder-related molecules in the hypothyroid mouse brain during the early postnatal period. Sci Rep. 2021;11:6723 pubmed publisher
  3. Lee M, Nam H, Kang H, Lee W, Lee G, Sung G, et al. Epigenetic regulation of p62/SQSTM1 overcomes the radioresistance of head and neck cancer cells via autophagy-dependent senescence induction. Cell Death Dis. 2021;12:250 pubmed publisher
  4. Zhang Y, Zhu Y, Cao S, Sun P, Yang J, Xia Y, et al. MeCP2 in cholinergic interneurons of nucleus accumbens regulates fear learning. elife. 2020;9: pubmed publisher
  5. Lavery L, Ure K, Wan Y, Luo C, Trostle A, Wang W, et al. Losing Dnmt3a dependent methylation in inhibitory neurons impairs neural function by a mechanism impacting Rett syndrome. elife. 2020;9: pubmed publisher
  6. Fukuoka M, Takahashi M, Fujita H, Chiyo T, Popiel H, Watanabe S, et al. Supplemental Treatment for Huntington's Disease with miR-132 that Is Deficient in Huntington's Disease Brain. Mol Ther Nucleic Acids. 2018;11:79-90 pubmed publisher
  7. Ka M, Kim W. ANKRD11 associated with intellectual disability and autism regulates dendrite differentiation via the BDNF/TrkB signaling pathway. Neurobiol Dis. 2018;111:138-152 pubmed publisher
  8. Liu Y, Zhang B, Meng X, Korn M, Parent J, Lu L, et al. UHRF2 regulates local 5-methylcytosine and suppresses spontaneous seizures. Epigenetics. 2017;12:551-560 pubmed publisher
  9. Maphis N, Jiang S, Binder J, Wright C, Gopalan B, Lamb B, et al. Whole Genome Expression Analysis in a Mouse Model of Tauopathy Identifies MECP2 as a Possible Regulator of Tau Pathology. Front Mol Neurosci. 2017;10:69 pubmed publisher
  10. Bu Q, Wang A, Hamzah H, Waldman A, Jiang K, Dong Q, et al. CREB Signaling Is Involved in Rett Syndrome Pathogenesis. J Neurosci. 2017;37:3671-3685 pubmed publisher
  11. Matagne V, Ehinger Y, Saidi L, Borges Correia A, Barkats M, Bartoli M, et al. A codon-optimized Mecp2 transgene corrects breathing deficits and improves survival in a mouse model of Rett syndrome. Neurobiol Dis. 2017;99:1-11 pubmed publisher
  12. Mahgoub M, Adachi M, Suzuki K, Liu X, Kavalali E, Chahrour M, et al. MeCP2 and histone deacetylases 1 and 2 in dorsal striatum collectively suppress repetitive behaviors. Nat Neurosci. 2016;19:1506-1512 pubmed publisher
  13. Williams A, Mehler V, Mueller C, Vonhoff F, White R, Duch C. Apoptotic Activity of MeCP2 Is Enhanced by C-Terminal Truncating Mutations. PLoS ONE. 2016;11:e0159632 pubmed publisher
  14. Meng X, Wang W, Lu H, He L, Chen W, Chao E, et al. Manipulations of MeCP2 in glutamatergic neurons highlight their contributions to Rett and other neurological disorders. elife. 2016;5: pubmed publisher
  15. Ure K, Lu H, Wang W, Ito Ishida A, Wu Z, He L, et al. Restoration of Mecp2 expression in GABAergic neurons is sufficient to rescue multiple disease features in a mouse model of Rett syndrome. elife. 2016;5: pubmed publisher
  16. Xu M, Bian S, Li J, He J, Chen H, Ge L, et al. MeCP2 suppresses LIN28A expression via binding to its methylated-CpG islands in pancreatic cancer cells. Oncotarget. 2016;7:14476-85 pubmed publisher
  17. Tai D, Liu Y, Hsu W, Ma Y, Cheng S, Liu S, et al. MeCP2 SUMOylation rescues Mecp2-mutant-induced behavioural deficits in a mouse model of Rett syndrome. Nat Commun. 2016;7:10552 pubmed publisher
  18. Kishi N, MacDonald J, Ye J, Molyneaux B, Azim E, Macklis J. Reduction of aberrant NF-κB signalling ameliorates Rett syndrome phenotypes in Mecp2-null mice. Nat Commun. 2016;7:10520 pubmed publisher
  19. Liu Z, Li X, Zhang J, Cai Y, Cheng T, Cheng C, et al. Autism-like behaviours and germline transmission in transgenic monkeys overexpressing MeCP2. Nature. 2016;530:98-102 pubmed publisher
  20. Tsuchiya Y, Minami Y, Umemura Y, Watanabe H, Ono D, Nakamura W, et al. Disruption of MeCP2 attenuates circadian rhythm in CRISPR/Cas9-based Rett syndrome model mouse. Genes Cells. 2015;20:992-1005 pubmed publisher
  21. Krishnan N, Krishnan K, Connors C, Choy M, Page R, Peti W, et al. PTP1B inhibition suggests a therapeutic strategy for Rett syndrome. J Clin Invest. 2015;125:3163-77 pubmed publisher
  22. Conti V, Gandaglia A, Galli F, Tirone M, Bellini E, Campana L, et al. MeCP2 Affects Skeletal Muscle Growth and Morphology through Non Cell-Autonomous Mechanisms. PLoS ONE. 2015;10:e0130183 pubmed publisher
  23. Bedogni F, Cobolli Gigli C, Pozzi D, Rossi R, Scaramuzza L, Rossetti G, et al. Defects During Mecp2 Null Embryonic Cortex Development Precede the Onset of Overt Neurological Symptoms. Cereb Cortex. 2016;26:2517-2529 pubmed publisher
  24. Zhang R, Huang M, Cao Z, Qi J, Qiu Z, Chiang L. MeCP2 plays an analgesic role in pain transmission through regulating CREB / miR-132 pathway. Mol Pain. 2015;11:19 pubmed publisher
  25. Hoefflin S, Carter D. Neuronal expression of SOX2 is enriched in specific hypothalamic cell groups. J Chem Neuroanat. 2014;61-62:153-60 pubmed publisher
  26. Subbanna S, Nagre N, Shivakumar M, Umapathy N, Psychoyos D, Basavarajappa B. Ethanol induced acetylation of histone at G9a exon1 and G9a-mediated histone H3 dimethylation leads to neurodegeneration in neonatal mice. Neuroscience. 2014;258:422-32 pubmed publisher
  27. Arora S, Saini S, Fukuhara S, Majid S, Shahryari V, Yamamura S, et al. MicroRNA-4723 inhibits prostate cancer growth through inactivation of the Abelson family of nonreceptor protein tyrosine kinases. PLoS ONE. 2013;8:e78023 pubmed publisher
  28. Cheng J, Huang M, Zhu Y, Xin Y, Zhao Y, Huang J, et al. SUMOylation of MeCP2 is essential for transcriptional repression and hippocampal synapse development. J Neurochem. 2014;128:798-806 pubmed publisher
  29. Ma P, Pan H, Montgomery R, Olson E, Schultz R. Compensatory functions of histone deacetylase 1 (HDAC1) and HDAC2 regulate transcription and apoptosis during mouse oocyte development. Proc Natl Acad Sci U S A. 2012;109:E481-9 pubmed publisher