This is a Validated Antibody Database (VAD) review about rat Lrrk2, based on 21 published articles (read how Labome selects the articles), using Lrrk2 antibody in all methods. It is aimed to help Labome visitors find the most suited Lrrk2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Lrrk2 synonym: leucine-rich repeat serine/threonine-protein kinase 2

Knockout validation
Abcam
rabbit monoclonal (MJFF2 (c41-2))
  • western blot knockout validation; mouse; 1:2000; loading ...; fig s1a
Abcam Lrrk2 antibody (Abcam, ab133474) was used in western blot knockout validation on mouse samples at 1:2000 (fig s1a). NPJ Parkinsons Dis (2018) ncbi
Abcam
rabbit monoclonal (MJFF2 (c41-2))
  • western blot knockout validation; human; loading ...; fig 5b
  • immunoprecipitation; human; loading ...; fig 6e
In order to find that LRRK2 regulates Rip2 and promotes inflammatory cytokine induction through the Nod1/2-Rip2 pathway, Abcam Lrrk2 antibody (Epitomics, MJFF2 c41-2) was used in western blot knockout validation on human samples (fig 5b) and in immunoprecipitation on human samples (fig 6e). Protein Cell (2017) ncbi
Abcam
rabbit monoclonal (MJFF2 (c41-2))
  • western blot knockout validation; mouse; 1:2000; fig 7
Abcam Lrrk2 antibody (Epitomics, MJFF2) was used in western blot knockout validation on mouse samples at 1:2000 (fig 7). J Biol Chem (2014) ncbi
Abcam
rabbit monoclonal (MJFF2 (c41-2))
  • immunohistochemistry knockout validation; mouse; fig 4
  • western blot knockout validation; mouse; fig 1, s2
  • immunohistochemistry - free floating section; mouse; fig 4
  • western blot; human; fig 1
  • immunohistochemistry knockout validation; rat; fig 6
  • immunohistochemistry - free floating section; rat; fig 6
Abcam Lrrk2 antibody (Abcam, ab133474) was used in immunohistochemistry knockout validation on mouse samples (fig 4), in western blot knockout validation on mouse samples (fig 1, s2), in immunohistochemistry - free floating section on mouse samples (fig 4), in western blot on human samples (fig 1), in immunohistochemistry knockout validation on rat samples (fig 6) and in immunohistochemistry - free floating section on rat samples (fig 6). Biochem J (2013) ncbi
Abcam
rabbit monoclonal (MJFF4)
  • immunocytochemistry knockout validation; mouse; fig s3
  • immunohistochemistry knockout validation; mouse; fig 4
  • western blot; mouse; fig 1
  • western blot; human; fig 1
Abcam Lrrk2 antibody (Abcam, ab133476) was used in immunocytochemistry knockout validation on mouse samples (fig s3), in immunohistochemistry knockout validation on mouse samples (fig 4), in western blot on mouse samples (fig 1) and in western blot on human samples (fig 1). Biochem J (2013) ncbi
Abcam
rabbit monoclonal (MJFF2 (c41-2))
  • immunohistochemistry knockout validation; mouse; 1:4000; fig 1
Abcam Lrrk2 antibody (Epitomics, MJFF2 (c41-2)) was used in immunohistochemistry knockout validation on mouse samples at 1:4000 (fig 1). Mol Neurodegener (2012) ncbi
Abcam
rabbit monoclonal (MJFF2 (c41-2))
  • western blot knockout validation; mouse; 1:2000; loading ...; fig s1a
Abcam Lrrk2 antibody (Abcam, ab133474) was used in western blot knockout validation on mouse samples at 1:2000 (fig s1a). NPJ Parkinsons Dis (2018) ncbi
rabbit monoclonal (MJFF2 (c41-2))
  • immunohistochemistry - frozen section; mouse; loading ...; fig s3e
Abcam Lrrk2 antibody (Abcam, ab133474) was used in immunohistochemistry - frozen section on mouse samples (fig s3e). Proc Natl Acad Sci U S A (2018) ncbi
rabbit monoclonal (MJFF2 (c41-2))
  • western blot; mouse; 1:300; loading ...; fig 1
In order to elucidate the effects of mutations in leucine-rich repeat kinase 2 on nigro0striatal dopamine neurons, Abcam Lrrk2 antibody (Abcam, ab133474) was used in western blot on mouse samples at 1:300 (fig 1). Acta Neuropathol Commun (2017) ncbi
rabbit monoclonal (MJFF2 (c41-2))
  • western blot knockout validation; human; loading ...; fig 5b
  • immunoprecipitation; human; loading ...; fig 6e
In order to find that LRRK2 regulates Rip2 and promotes inflammatory cytokine induction through the Nod1/2-Rip2 pathway, Abcam Lrrk2 antibody (Epitomics, MJFF2 c41-2) was used in western blot knockout validation on human samples (fig 5b) and in immunoprecipitation on human samples (fig 6e). Protein Cell (2017) ncbi
rabbit monoclonal (MJFF2 (c41-2))
  • western blot; human; fig 2b
Abcam Lrrk2 antibody (Abcam, ab133474) was used in western blot on human samples (fig 2b). PLoS ONE (2016) ncbi
rabbit monoclonal (MJFF3 (c69-6))
  • immunohistochemistry; zebrafish ; 1:200; loading ...; fig 6a
  • western blot; zebrafish ; 1:500; loading ...; fig 1a
In order to describe multifaceted roles of LRRK2 in zebrafish, Abcam Lrrk2 antibody (Abcam, ab133475) was used in immunohistochemistry on zebrafish samples at 1:200 (fig 6a) and in western blot on zebrafish samples at 1:500 (fig 1a). J Neurosci Res (2016) ncbi
rabbit monoclonal (MJFF2 (c41-2))
  • western blot; human; 1:2000; loading ...; fig 1c
Abcam Lrrk2 antibody (Abcam, ab133474) was used in western blot on human samples at 1:2000 (fig 1c). Mol Neurobiol (2016) ncbi
rabbit monoclonal (MJFF2 (c41-2))
  • immunoprecipitation; human; fig 2a
  • western blot; human; fig 2b
Abcam Lrrk2 antibody (Abcam, ab133474) was used in immunoprecipitation on human samples (fig 2a) and in western blot on human samples (fig 2b). Mol Brain (2015) ncbi
rabbit monoclonal (MJFF2 (c41-2))
  • western blot; mouse; 1:1000; fig 4
  • western blot; human; 1:1000; fig 3
  • western blot; rat; 1:1000; fig 3
Abcam Lrrk2 antibody (Abcam, ab133474) was used in western blot on mouse samples at 1:1000 (fig 4), in western blot on human samples at 1:1000 (fig 3) and in western blot on rat samples at 1:1000 (fig 3). Nat Commun (2015) ncbi
rabbit monoclonal (MJFF2 (c41-2))
  • western blot; human; 1:3000; fig 1c
  • western blot; mouse; 1:3000; fig 1d
In order to analyze protein kinase LRRK2, parkinson's disease associated, which modulates notch signaling via the endosomal pathway, Abcam Lrrk2 antibody (Abcam, MJFF2) was used in western blot on human samples at 1:3000 (fig 1c) and in western blot on mouse samples at 1:3000 (fig 1d). PLoS Genet (2015) ncbi
rabbit monoclonal (MJFF4)
  • western blot; mouse; 1:1000; fig 2d
In order to compare cognitive and motor behaviors in various LRRK2 transgenic mice, Abcam Lrrk2 antibody (Abcam, ab133476) was used in western blot on mouse samples at 1:1000 (fig 2d). Parkinsonism Relat Disord (2015) ncbi
rabbit monoclonal (MJFF2 (c41-2))
  • western blot; human
In order to elucidate the relationship between LRRK2 and Rab5b, Abcam Lrrk2 antibody (Abcam, ab133474) was used in western blot on human samples . J Biochem (2015) ncbi
rabbit monoclonal (MJFF2 (c41-2))
  • western blot; human; 1:1000; fig 3
Abcam Lrrk2 antibody (Abcam, ab133474) was used in western blot on human samples at 1:1000 (fig 3). Biomed Res Int (2014) ncbi
rabbit monoclonal (MJFF4)
  • western blot; rat
In order to examine the p.G2019S mutation in the leucine-rich repeat kinase 2 (LRRK2) and it's role in Parkinson's disease, Abcam Lrrk2 antibody (Abcam, ab133476) was used in western blot on rat samples . J Parkinsons Dis (2014) ncbi
rabbit monoclonal (MJFF2 (c41-2))
  • western blot knockout validation; mouse; 1:2000; fig 7
Abcam Lrrk2 antibody (Epitomics, MJFF2) was used in western blot knockout validation on mouse samples at 1:2000 (fig 7). J Biol Chem (2014) ncbi
rabbit monoclonal (MJFF2 (c41-2))
  • immunohistochemistry knockout validation; mouse; fig 4
  • western blot knockout validation; mouse; fig 1, s2
  • immunohistochemistry - free floating section; mouse; fig 4
  • western blot; human; fig 1
  • immunohistochemistry knockout validation; rat; fig 6
  • immunohistochemistry - free floating section; rat; fig 6
Abcam Lrrk2 antibody (Abcam, ab133474) was used in immunohistochemistry knockout validation on mouse samples (fig 4), in western blot knockout validation on mouse samples (fig 1, s2), in immunohistochemistry - free floating section on mouse samples (fig 4), in western blot on human samples (fig 1), in immunohistochemistry knockout validation on rat samples (fig 6) and in immunohistochemistry - free floating section on rat samples (fig 6). Biochem J (2013) ncbi
rabbit monoclonal (MJFF4)
  • immunocytochemistry knockout validation; mouse; fig s3
  • immunohistochemistry knockout validation; mouse; fig 4
  • western blot; mouse; fig 1
  • western blot; human; fig 1
Abcam Lrrk2 antibody (Abcam, ab133476) was used in immunocytochemistry knockout validation on mouse samples (fig s3), in immunohistochemistry knockout validation on mouse samples (fig 4), in western blot on mouse samples (fig 1) and in western blot on human samples (fig 1). Biochem J (2013) ncbi
rabbit monoclonal (MJFF2 (c41-2))
  • immunohistochemistry knockout validation; mouse; 1:4000; fig 1
Abcam Lrrk2 antibody (Epitomics, MJFF2 (c41-2)) was used in immunohistochemistry knockout validation on mouse samples at 1:4000 (fig 1). Mol Neurodegener (2012) ncbi
Novus Biologicals
rabbit polyclonal
  • western blot; human; 1:1000; fig 3c
In order to discuss the contribution of leucine-rich repeat kinase 2 mutations to Parkinson's disease, Novus Biologicals Lrrk2 antibody (Novus Biologicals, NB110-58771) was used in western blot on human samples at 1:1000 (fig 3c). J Neuroinflammation (2016) ncbi
Cell Signaling Technology
rabbit polyclonal
  • western blot; rat; loading ...; fig 4f
Cell Signaling Technology Lrrk2 antibody (Cell Signaling Technology, 5559) was used in western blot on rat samples (fig 4f). Am J Physiol Endocrinol Metab (2017) ncbi
rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 4d
In order to discuss the contribution of LINK-A to cancer, Cell Signaling Technology Lrrk2 antibody (Cell Signaling, 5559) was used in western blot on human samples at 1:1000 (fig 4d). Nat Cell Biol (2016) ncbi
rabbit polyclonal
  • western blot; human; fig s4
In order to study the effect of G2019S on neuronal subtypes, Cell Signaling Technology Lrrk2 antibody (Cell Signal, 5559) was used in western blot on human samples (fig s4). Stem Cell Reports (2015) ncbi
Articles Reviewed
  1. Kluss J, Conti M, Kaganovich A, Beilina A, Melrose H, Cookson M, et al. Detection of endogenous S1292 LRRK2 autophosphorylation in mouse tissue as a readout for kinase activity. NPJ Parkinsons Dis. 2018;4:13 pubmed publisher
  2. Xiong Y, Neifert S, Karuppagounder S, Liu Q, Stankowski J, Lee B, et al. Robust kinase- and age-dependent dopaminergic and norepinephrine neurodegeneration in LRRK2 G2019S transgenic mice. Proc Natl Acad Sci U S A. 2018;115:1635-1640 pubmed publisher
  3. Pinto S, Lamon S, Stephenson E, Kalanon M, Mikovic J, Koch L, et al. Expression of microRNAs and target proteins in skeletal muscle of rats selectively bred for high and low running capacity. Am J Physiol Endocrinol Metab. 2017;313:E335-E343 pubmed publisher
  4. Longo F, Mercatelli D, Novello S, Arcuri L, Brugnoli A, Vincenzi F, et al. Age-dependent dopamine transporter dysfunction and Serine129 phospho-α-synuclein overload in G2019S LRRK2 mice. Acta Neuropathol Commun. 2017;5:22 pubmed publisher
  5. López de Maturana R, Lang V, Zubiarrain A, Sousa A, Vázquez N, Gorostidi A, et al. Mutations in LRRK2 impair NF-κB pathway in iPSC-derived neurons. J Neuroinflammation. 2016;13:295 pubmed
  6. Yan R, Liu Z. LRRK2 enhances Nod1/2-mediated inflammatory cytokine production by promoting Rip2 phosphorylation. Protein Cell. 2017;8:55-66 pubmed publisher
  7. Speidel A, Felk S, Reinhardt P, Sterneckert J, Gillardon F. Leucine-Rich Repeat Kinase 2 Influences Fate Decision of Human Monocytes Differentiated from Induced Pluripotent Stem Cells. PLoS ONE. 2016;11:e0165949 pubmed publisher
  8. Prabhudesai S, Bensabeur F, Abdullah R, Basak I, Baez S, Alves G, et al. LRRK2 knockdown in zebrafish causes developmental defects, neuronal loss, and synuclein aggregation. J Neurosci Res. 2016;94:717-35 pubmed publisher
  9. Lin A, Li C, Xing Z, Hu Q, Liang K, Han L, et al. The LINK-A lncRNA activates normoxic HIF1? signalling in triple-negative breast cancer. Nat Cell Biol. 2016;18:213-24 pubmed publisher
  10. Schwab A, Ebert A. Neurite Aggregation and Calcium Dysfunction in iPSC-Derived Sensory Neurons with Parkinson's Disease-Related LRRK2 G2019S Mutation. Stem Cell Reports. 2015;5:1039-1052 pubmed publisher
  11. Smith G, Jansson J, Rocha E, Osborn T, Hallett P, Isacson O. Fibroblast Biomarkers of Sporadic Parkinson's Disease and LRRK2 Kinase Inhibition. Mol Neurobiol. 2016;53:5161-77 pubmed publisher
  12. Ho D, Kim H, Kim J, Sim H, Ahn H, Kim J, et al. Leucine-Rich Repeat Kinase 2 (LRRK2) phosphorylates p53 and induces p21(WAF1/CIP1) expression. Mol Brain. 2015;8:54 pubmed publisher
  13. Choi I, Kim B, Byun J, Baik S, Huh Y, Kim J, et al. LRRK2 G2019S mutation attenuates microglial motility by inhibiting focal adhesion kinase. Nat Commun. 2015;6:8255 pubmed publisher
  14. Imai Y, Kobayashi Y, Inoshita T, Meng H, Arano T, Uemura K, et al. The Parkinson's Disease-Associated Protein Kinase LRRK2 Modulates Notch Signaling through the Endosomal Pathway. PLoS Genet. 2015;11:e1005503 pubmed publisher
  15. Volta M, Cataldi S, Beccano Kelly D, Munsie L, Tatarnikov I, Chou P, et al. Chronic and acute LRRK2 silencing has no long-term behavioral effects, whereas wild-type and mutant LRRK2 overexpression induce motor and cognitive deficits and altered regulation of dopamine release. Parkinsonism Relat Disord. 2015;21:1156-63 pubmed publisher
  16. Yun H, Kim H, Ga I, Oh H, Ho D, Kim J, et al. An early endosome regulator, Rab5b, is an LRRK2 kinase substrate. J Biochem. 2015;157:485-95 pubmed publisher
  17. Ho D, Yi S, Seo H, Son I, Seol W. Increased DJ-1 in urine exosome of Korean males with Parkinson's disease. Biomed Res Int. 2014;2014:704678 pubmed publisher
  18. Walker M, Volta M, Cataldi S, Dinelle K, Beccano Kelly D, Munsie L, et al. Behavioral deficits and striatal DA signaling in LRRK2 p.G2019S transgenic rats: a multimodal investigation including PET neuroimaging. J Parkinsons Dis. 2014;4:483-98 pubmed publisher
  19. Law B, Spain V, Leinster V, Chia R, Beilina A, Cho H, et al. A direct interaction between leucine-rich repeat kinase 2 and specific ?-tubulin isoforms regulates tubulin acetylation. J Biol Chem. 2014;289:895-908 pubmed publisher
  20. Davies P, Hinkle K, Sukar N, Sepulveda B, Mesias R, Serrano G, et al. Comprehensive characterization and optimization of anti-LRRK2 (leucine-rich repeat kinase 2) monoclonal antibodies. Biochem J. 2013;453:101-13 pubmed publisher
  21. Hinkle K, Yue M, Behrouz B, Dachsel J, Lincoln S, Bowles E, et al. LRRK2 knockout mice have an intact dopaminergic system but display alterations in exploratory and motor co-ordination behaviors. Mol Neurodegener. 2012;7:25 pubmed publisher