This is a Validated Antibody Database (VAD) review about rat Lrp6, based on 31 published articles (read how Labome selects the articles), using Lrp6 antibody in all methods. It is aimed to help Labome visitors find the most suited Lrp6 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Knockout validation
Cell Signaling Technology
domestic rabbit monoclonal (C47E12)
  • western blot knockout validation; human; 1:1000; loading ...; fig 6f
Cell Signaling Technology Lrp6 antibody (Cell Signaling, 3395) was used in western blot knockout validation on human samples at 1:1000 (fig 6f). Cell Rep (2019) ncbi
Santa Cruz Biotechnology
mouse monoclonal (C-10)
  • western blot; human; loading ...; fig s3i
Santa Cruz Biotechnology Lrp6 antibody (SantaCruz, sc-25317) was used in western blot on human samples (fig s3i). Nature (2017) ncbi
mouse monoclonal (C-10)
  • immunoprecipitation; mouse; loading ...; fig 5c
  • immunohistochemistry; mouse; 1:50; loading ...; fig s6e
  • western blot; mouse; 1:500; loading ...; fig s7b
  • immunocytochemistry; human; 1:500; loading ...; fig s6f
In order to demonstrate that actin-binding protein filamin A interacts with actin-nucleating protein formin 2, Santa Cruz Biotechnology Lrp6 antibody (Santa Cruz, Sc-25317) was used in immunoprecipitation on mouse samples (fig 5c), in immunohistochemistry on mouse samples at 1:50 (fig s6e), in western blot on mouse samples at 1:500 (fig s7b) and in immunocytochemistry on human samples at 1:500 (fig s6f). Development (2016) ncbi
mouse monoclonal (C-10)
  • western blot; human; fig 2
Santa Cruz Biotechnology Lrp6 antibody (Santa Cruz, sc-25317) was used in western blot on human samples (fig 2). Int J Mol Sci (2016) ncbi
mouse monoclonal
  • western blot; human; 1:500; tbl 1
In order to research the expression and the functions of cancer/testis-antigens in seminomas, Santa Cruz Biotechnology Lrp6 antibody (Santa Cruz, C10) was used in western blot on human samples at 1:500 (tbl 1). Br J Cancer (2016) ncbi
mouse monoclonal
  • immunohistochemistry; mouse; loading ...; fig s2a
  • immunohistochemistry; human; loading ...; fig s2c
In order to research the atheroprotective effect of OCT4 in smooth muscle cells, Santa Cruz Biotechnology Lrp6 antibody (Santa-Cruz, C10) was used in immunohistochemistry on mouse samples (fig s2a) and in immunohistochemistry on human samples (fig s2c). Nat Med (2016) ncbi
mouse monoclonal (C-10)
  • western blot; human; fig s2
Santa Cruz Biotechnology Lrp6 antibody (Santa Cruz, sc-25317) was used in western blot on human samples (fig s2). Int J Mol Sci (2016) ncbi
Abcam
domestic rabbit monoclonal (EPR2423(2))
  • flow cytometry; mouse; 1:600; loading ...; fig 5e
  • western blot; mouse; 1:500; loading ...; fig 5d
Abcam Lrp6 antibody (Abcam, ab134146) was used in flow cytometry on mouse samples at 1:600 (fig 5e) and in western blot on mouse samples at 1:500 (fig 5d). Bone Res (2018) ncbi
domestic rabbit monoclonal (EPR2423(2))
  • western blot; rat; loading ...; fig 5
Abcam Lrp6 antibody (Abcam, EPR2423(2)) was used in western blot on rat samples (fig 5). Biomed Pharmacother (2018) ncbi
domestic rabbit monoclonal (EPR2423(2))
  • immunocytochemistry; human; 1:100; loading ...; fig s6f
  • immunohistochemistry; mouse; 1:100; loading ...; fig s6e
  • western blot; mouse; 1:1000; loading ...; fig s7b
In order to demonstrate that actin-binding protein filamin A interacts with actin-nucleating protein formin 2, Abcam Lrp6 antibody (Abcam, ab134146) was used in immunocytochemistry on human samples at 1:100 (fig s6f), in immunohistochemistry on mouse samples at 1:100 (fig s6e) and in western blot on mouse samples at 1:1000 (fig s7b). Development (2016) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (C5C7)
  • western blot; human; loading ...; fig 7d
Cell Signaling Technology Lrp6 antibody (Cell signaling Technology, 2560) was used in western blot on human samples (fig 7d). Mol Cancer (2020) ncbi
domestic rabbit monoclonal (C47E12)
  • western blot knockout validation; human; 1:1000; loading ...; fig 6f
Cell Signaling Technology Lrp6 antibody (Cell Signaling, 3395) was used in western blot knockout validation on human samples at 1:1000 (fig 6f). Cell Rep (2019) ncbi
domestic rabbit monoclonal (C5C7)
  • western blot; rat; loading ...; fig 4a
Cell Signaling Technology Lrp6 antibody (Cell Signaling, C5C7) was used in western blot on rat samples (fig 4a). Biomed Pharmacother (2018) ncbi
domestic rabbit monoclonal (C5C7)
  • western blot; human; loading ...; fig 1b
Cell Signaling Technology Lrp6 antibody (Cell Signaling Technology, 2560) was used in western blot on human samples (fig 1b). EMBO J (2018) ncbi
domestic rabbit monoclonal (C47E12)
  • western blot; human; loading ...; fig s6g
Cell Signaling Technology Lrp6 antibody (Cell Signaling, 3395) was used in western blot on human samples (fig s6g). Nature (2017) ncbi
domestic rabbit monoclonal (C5C7)
  • western blot; human; loading ...; fig 2a
Cell Signaling Technology Lrp6 antibody (Cell Signaling, C5C7) was used in western blot on human samples (fig 2a). Oncotarget (2017) ncbi
domestic rabbit monoclonal (C47E12)
  • western blot; human; loading ...; fig 4a
Cell Signaling Technology Lrp6 antibody (Cell Signaling, C47E12) was used in western blot on human samples (fig 4a). Proc Natl Acad Sci U S A (2017) ncbi
domestic rabbit monoclonal (C5C7)
  • western blot; human; loading ...; fig 1b
In order to describe the dual roles of TbetaRIII/betaglycan, Cell Signaling Technology Lrp6 antibody (Cell signaling, 2560) was used in western blot on human samples (fig 1b). J Biol Chem (2016) ncbi
domestic rabbit monoclonal (C5C7)
  • western blot; human; loading ...; fig s9b
In order to report that frizzled family proteins are receptors for Clostridium difficile toxin B, Cell Signaling Technology Lrp6 antibody (Cell Signaling, C5C7) was used in western blot on human samples (fig s9b). Nature (2016) ncbi
domestic rabbit monoclonal (C47E12)
  • western blot; human; 1:1000; fig st1
In order to identify and characterize alterations in signal transduction that occur during the development Lapatinib resistance, Cell Signaling Technology Lrp6 antibody (Cell Signaling, 3395) was used in western blot on human samples at 1:1000 (fig st1). Nat Commun (2016) ncbi
domestic rabbit monoclonal (C47E12)
  • western blot; mouse; fig 2
In order to determine maintenance of mouse embryonic stem cell identity by a myc-driven self-reinforcing regulatory network, Cell Signaling Technology Lrp6 antibody (Cell Signalling, 3395) was used in western blot on mouse samples (fig 2). Nat Commun (2016) ncbi
domestic rabbit monoclonal (C47E12)
  • western blot; mouse; fig 1
Cell Signaling Technology Lrp6 antibody (Cell Signaling, 3395S) was used in western blot on mouse samples (fig 1). PLoS Genet (2016) ncbi
domestic rabbit monoclonal (C47E12)
  • western blot; human; loading ...; fig 3f
Cell Signaling Technology Lrp6 antibody (Cell Signaling, 3395S) was used in western blot on human samples (fig 3f). Proc Natl Acad Sci U S A (2016) ncbi
domestic rabbit monoclonal (C5C7)
  • western blot; human; 1:1000; fig 6
Cell Signaling Technology Lrp6 antibody (Cell Signaling, 2560) was used in western blot on human samples at 1:1000 (fig 6). Nat Commun (2016) ncbi
domestic rabbit monoclonal (C47E12)
  • western blot; mouse
Cell Signaling Technology Lrp6 antibody (Cell Signaling Technology, 3395) was used in western blot on mouse samples . PLoS ONE (2014) ncbi
domestic rabbit monoclonal (C5C7)
  • western blot; rat; loading ...; fig 3
Cell Signaling Technology Lrp6 antibody (Cell Signaling, 2560) was used in western blot on rat samples (fig 3). Biomed Res Int (2014) ncbi
domestic rabbit monoclonal (C47E12)
  • western blot; human; fig 5
Cell Signaling Technology Lrp6 antibody (Cell Signaling Technology, C47E12) was used in western blot on human samples (fig 5). Cell (2014) ncbi
domestic rabbit monoclonal (C5C7)
  • western blot; human; fig 6
In order to identify serines in the beta-catenin-binding domain of E-cadherin that are phosphorylated, Cell Signaling Technology Lrp6 antibody (Cell Signaling, C5C7) was used in western blot on human samples (fig 6). Mol Biol Cell (2014) ncbi
domestic rabbit monoclonal (C5C7)
  • western blot; human
Cell Signaling Technology Lrp6 antibody (CST, 2560) was used in western blot on human samples . FEBS Lett (2014) ncbi
domestic rabbit monoclonal (C5C7)
  • western blot; human; 1:1000
In order to investigate the interaction between alpha-taxilin and nexin 4 and its role in the recycling pathway of transferrin receptor, Cell Signaling Technology Lrp6 antibody (Cell Signaling, 2560S) was used in western blot on human samples at 1:1000. PLoS ONE (2014) ncbi
domestic rabbit monoclonal (C5C7)
  • western blot; human; 1:1000
Cell Signaling Technology Lrp6 antibody (Cell Signaling, C5C7) was used in western blot on human samples at 1:1000. BMC Cell Biol (2013) ncbi
domestic rabbit monoclonal (C5C7)
  • western blot; human
Cell Signaling Technology Lrp6 antibody (Cell Signaling Technology, C5C7) was used in western blot on human samples . Cell Mol Life Sci (2014) ncbi
domestic rabbit monoclonal (C5C7)
  • immunohistochemistry - paraffin section; human; 1:200
In order to study the expression and prognostic value of Wnt and TGF-beta pathway components and key adrenal transcription factors in adrenocortical tumors, Cell Signaling Technology Lrp6 antibody (Cell, 2560S) was used in immunohistochemistry - paraffin section on human samples at 1:200. Pathol Res Pract (2013) ncbi
domestic rabbit monoclonal (C5C7)
  • western blot; mouse; fig 3
In order to investigate the contribution of Rspo/Wnt on gene expression, Cell Signaling Technology Lrp6 antibody (Cell Signaling Technology, 2560) was used in western blot on mouse samples (fig 3). PLoS ONE (2012) ncbi
Articles Reviewed
  1. Pothuraju R, Rachagani S, Krishn S, Chaudhary S, Nimmakayala R, Siddiqui J, et al. Molecular implications of MUC5AC-CD44 axis in colorectal cancer progression and chemoresistance. Mol Cancer. 2020;19:37 pubmed publisher
  2. Agajanian M, Walker M, Axtman A, Ruela de Sousa R, Serafin D, Rabinowitz A, et al. WNT Activates the AAK1 Kinase to Promote Clathrin-Mediated Endocytosis of LRP6 and Establish a Negative Feedback Loop. Cell Rep. 2019;26:79-93.e8 pubmed publisher
  3. Wang L, Chai Y, Li C, Liu H, Su W, Liu X, et al. Oxidized phospholipids are ligands for LRP6. Bone Res. 2018;6:22 pubmed publisher
  4. Chen Z, Li Y, Jiang G, Yang C, Wang Y, Wang X, et al. Knockdown of LRP6 activates Drp1 to inhibit survival of cardiomyocytes during glucose deprivation. Biomed Pharmacother. 2018;103:1408-1414 pubmed publisher
  5. Glaeser K, Urban M, Fenech E, Voloshanenko O, Kranz D, Lari F, et al. ERAD-dependent control of the Wnt secretory factor Evi. EMBO J. 2018;37: pubmed publisher
  6. Wang H, Nicolay B, Chick J, Gao X, Geng Y, Ren H, et al. The metabolic function of cyclin D3-CDK6 kinase in cancer cell survival. Nature. 2017;546:426-430 pubmed publisher
  7. Janda C, Dang L, You C, Chang J, de Lau W, Zhong Z, et al. Surrogate Wnt agonists that phenocopy canonical Wnt and ?-catenin signalling. Nature. 2017;545:234-237 pubmed publisher
  8. Nag J, Kancharla A, Maoz M, Turm H, Agranovich D, Gupta C, et al. Low-density lipoprotein receptor-related protein 6 is a novel coreceptor of protease-activated receptor-2 in the dynamics of cancer-associated ?-catenin stabilization. Oncotarget. 2017;8:38650-38667 pubmed publisher
  9. van Andel H, Ren Z, Koopmans I, Joosten S, Kocemba K, de Lau W, et al. Aberrantly expressed LGR4 empowers Wnt signaling in multiple myeloma by hijacking osteoblast-derived R-spondins. Proc Natl Acad Sci U S A. 2017;114:376-381 pubmed publisher
  10. Lian G, Dettenhofer M, Lu J, Downing M, Chenn A, Wong T, et al. Filamin A- and formin 2-dependent endocytosis regulates proliferation via the canonical Wnt pathway. Development. 2016;143:4509-4520 pubmed
  11. JENKINS L, Singh P, Varadaraj A, Lee N, Shah S, Flores H, et al. Altering the Proteoglycan State of Transforming Growth Factor ? Type III Receptor (T?RIII)/Betaglycan Modulates Canonical Wnt/?-Catenin Signaling. J Biol Chem. 2016;291:25716-25728 pubmed
  12. Tao L, Zhang J, Meraner P, Tovaglieri A, Wu X, Gerhard R, et al. Frizzled proteins are colonic epithelial receptors for C. difficile toxin B. Nature. 2016;538:350-355 pubmed publisher
  13. Treindl F, Ruprecht B, Beiter Y, Schultz S, Döttinger A, Staebler A, et al. A bead-based western for high-throughput cellular signal transduction analyses. Nat Commun. 2016;7:12852 pubmed publisher
  14. Guo J, Li Y, Ren Y, Sun Z, Dong J, Yan H, et al. Mutant LRP6 Impairs Endothelial Cell Functions Associated with Familial Normolipidemic Coronary Artery Disease. Int J Mol Sci. 2016;17: pubmed publisher
  15. Nettersheim D, Arndt I, Sharma R, Riesenberg S, Jostes S, Schneider S, et al. The cancer/testis-antigen PRAME supports the pluripotency network and represses somatic and germ cell differentiation programs in seminomas. Br J Cancer. 2016;115:454-64 pubmed publisher
  16. Fagnocchi L, Cherubini A, Hatsuda H, Fasciani A, Mazzoleni S, Poli V, et al. A Myc-driven self-reinforcing regulatory network maintains mouse embryonic stem cell identity. Nat Commun. 2016;7:11903 pubmed publisher
  17. Zeng L, Cai C, Li S, Wang W, Li Y, Chen J, et al. Essential Roles of Cyclin Y-Like 1 and Cyclin Y in Dividing Wnt-Responsive Mammary Stem/Progenitor Cells. PLoS Genet. 2016;12:e1006055 pubmed publisher
  18. Cherepanova O, Gomez D, Shankman L, Swiatlowska P, Williams J, Sarmento O, et al. Activation of the pluripotency factor OCT4 in smooth muscle cells is atheroprotective. Nat Med. 2016;22:657-65 pubmed publisher
  19. Madan B, Walker M, Young R, Quick L, Orgel K, Ryan M, et al. USP6 oncogene promotes Wnt signaling by deubiquitylating Frizzleds. Proc Natl Acad Sci U S A. 2016;113:E2945-54 pubmed publisher
  20. Yang E, Tacchelly Benites O, Wang Z, Randall M, Tian A, Benchabane H, et al. Wnt pathway activation by ADP-ribosylation. Nat Commun. 2016;7:11430 pubmed publisher
  21. Ozeki N, Mogi M, Hase N, Hiyama T, Yamaguchi H, Kawai R, et al. Wnt16 Signaling Is Required for IL-1β-Induced Matrix Metalloproteinase-13-Regulated Proliferation of Human Stem Cell-Derived Osteoblastic Cells. Int J Mol Sci. 2016;17:221 pubmed publisher
  22. Willis C, Klüppel M. Chondroitin sulfate-E is a negative regulator of a pro-tumorigenic Wnt/beta-catenin-Collagen 1 axis in breast cancer cells. PLoS ONE. 2014;9:e103966 pubmed publisher
  23. Bañón Maneus E, Rovira J, Ramírez Bajo M, Moya Rull D, Hierro Garcia N, Takenaka S, et al. Wnt pathway activation in long term remnant rat model. Biomed Res Int. 2014;2014:324713 pubmed publisher
  24. Azzolin L, Panciera T, Soligo S, Enzo E, Bicciato S, Dupont S, et al. YAP/TAZ incorporation in the ?-catenin destruction complex orchestrates the Wnt response. Cell. 2014;158:157-70 pubmed publisher
  25. McEwen A, Maher M, Mo R, Gottardi C. E-cadherin phosphorylation occurs during its biosynthesis to promote its cell surface stability and adhesion. Mol Biol Cell. 2014;25:2365-74 pubmed publisher
  26. Li S, Song W, Jiang M, Zeng L, Zhu X, Chen J. Phosphorylation of cyclin Y by CDK14 induces its ubiquitination and degradation. FEBS Lett. 2014;588:1989-96 pubmed publisher
  27. Sakane H, Horii Y, Nogami S, Kawano Y, Kaneko Kawano T, Shirataki H. ?-Taxilin interacts with sorting nexin 4 and participates in the recycling pathway of transferrin receptor. PLoS ONE. 2014;9:e93509 pubmed publisher
  28. Ogunkolade B, Jones T, Aarum J, Szary J, Owen N, Ottaviani D, et al. BORIS/CTCFL is an RNA-binding protein that associates with polysomes. BMC Cell Biol. 2013;14:52 pubmed publisher
  29. Knoblich K, Wang H, Sharma C, Fletcher A, Turley S, Hemler M. Tetraspanin TSPAN12 regulates tumor growth and metastasis and inhibits ?-catenin degradation. Cell Mol Life Sci. 2014;71:1305-14 pubmed publisher
  30. Parviainen H, Schrade A, Kiiveri S, Prunskaite Hyyryläinen R, Haglund C, Vainio S, et al. Expression of Wnt and TGF-? pathway components and key adrenal transcription factors in adrenocortical tumors: association to carcinoma aggressiveness. Pathol Res Pract. 2013;209:503-9 pubmed publisher
  31. Baljinnyam B, Klauzinska M, Saffo S, Callahan R, Rubin J. Recombinant R-spondin2 and Wnt3a up- and down-regulate novel target genes in C57MG mouse mammary epithelial cells. PLoS ONE. 2012;7:e29455 pubmed publisher