This is a Validated Antibody Database (VAD) review about rat Lgals3, based on 35 published articles (read how Labome selects the articles), using Lgals3 antibody in all methods. It is aimed to help Labome visitors find the most suited Lgals3 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Lgals3 synonym: AGE-R3; CBP30; L-34; gal-3

Knockout validation
Santa Cruz Biotechnology
rat monoclonal (M3/38)
  • immunohistochemistry knockout validation; mouse; fig 7
  • western blot knockout validation; mouse; fig 5
  • immunohistochemistry - paraffin section; mouse; fig 7
Santa Cruz Biotechnology Lgals3 antibody (Santa Cruz, M3/38) was used in immunohistochemistry knockout validation on mouse samples (fig 7), in western blot knockout validation on mouse samples (fig 5) and in immunohistochemistry - paraffin section on mouse samples (fig 7). Kidney Int (2016) ncbi
Abcam
mouse monoclonal (A3A12)
  • immunohistochemistry - paraffin section; human; 1:100; loading ...; fig 1a
Abcam Lgals3 antibody (Abcam, ab2785) was used in immunohistochemistry - paraffin section on human samples at 1:100 (fig 1a). J Neuroinflammation (2022) ncbi
domestic rabbit monoclonal (EP2775Y)
  • western blot; human; loading ...; fig 2b
Abcam Lgals3 antibody (Abcam, ab76245) was used in western blot on human samples (fig 2b). Thorac Cancer (2022) ncbi
mouse monoclonal (A3A12)
  • immunohistochemistry - paraffin section; mouse; 1:50; fig 1h
Abcam Lgals3 antibody (Abcam, ab2785) was used in immunohistochemistry - paraffin section on mouse samples at 1:50 (fig 1h). Nat Commun (2022) ncbi
domestic rabbit monoclonal
  • immunohistochemistry; mouse; 1:200; fig 4a
Abcam Lgals3 antibody (Abcam, Ab76466) was used in immunohistochemistry on mouse samples at 1:200 (fig 4a). J Cell Mol Med (2021) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:200; fig 6a
Abcam Lgals3 antibody (Abcam, ab53082) was used in immunohistochemistry - paraffin section on mouse samples at 1:200 (fig 6a). BMC Musculoskelet Disord (2021) ncbi
domestic rabbit monoclonal (EP2775Y)
  • immunohistochemistry; human; 1:1000; loading ...; fig 2
Abcam Lgals3 antibody (Abcam, ab76245) was used in immunohistochemistry on human samples at 1:1000 (fig 2). Ren Fail (2021) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; mouse; loading ...; fig 6
Abcam Lgals3 antibody (abcam, ab53082) was used in immunohistochemistry on mouse samples (fig 6). Theranostics (2021) ncbi
mouse monoclonal (A3A12)
  • immunohistochemistry - paraffin section; rat; 1:100; loading ...; fig 7a
Abcam Lgals3 antibody (Abcam, ab2785) was used in immunohistochemistry - paraffin section on rat samples at 1:100 (fig 7a). Theranostics (2020) ncbi
domestic rabbit monoclonal (EP2775Y)
  • immunohistochemistry - paraffin section; human; 1:2000; loading ...; fig 1c
Abcam Lgals3 antibody (Abcam, ab76245) was used in immunohistochemistry - paraffin section on human samples at 1:2000 (fig 1c). EBioMedicine (2020) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 1a
Abcam Lgals3 antibody (Abcam, ab53082) was used in immunohistochemistry - paraffin section on mouse samples (fig 1a). Front Endocrinol (Lausanne) (2020) ncbi
domestic rabbit polyclonal
  • western blot; rat; loading ...; fig 4b
Abcam Lgals3 antibody (Abcam, ab53082) was used in western blot on rat samples (fig 4b). Mol Pain (2020) ncbi
domestic rabbit monoclonal (EP2775Y)
  • immunohistochemistry - paraffin section; rat; 1:250; loading ...; fig 4a
  • western blot; rat; 1:5000; loading ...; fig 4d
Abcam Lgals3 antibody (Abcam, ab76245) was used in immunohistochemistry - paraffin section on rat samples at 1:250 (fig 4a) and in western blot on rat samples at 1:5000 (fig 4d). Biosci Rep (2019) ncbi
mouse monoclonal (A3A12)
  • immunocytochemistry; mouse; loading ...; fig 7a
In order to find that Legionella pneumophila has evolved to modulate mechanistic target of rapamycin-dependent lipogenesis in order to replicate, Abcam Lgals3 antibody (Abcam, A3A12) was used in immunocytochemistry on mouse samples (fig 7a). PLoS Pathog (2016) ncbi
mouse monoclonal (A3A12)
  • western blot; human; 1:1000; fig 2
In order to determine reduction of osteo-inductive potential of human plasma derived extracellular vesicles by a decrease in vesicular galectin-3 levels that decreses with donor age, Abcam Lgals3 antibody (Abcam, ab2785) was used in western blot on human samples at 1:1000 (fig 2). Aging (Albany NY) (2016) ncbi
mouse monoclonal (A3A12)
  • immunocytochemistry; human; 1:200
Abcam Lgals3 antibody (Abcam, ab2785) was used in immunocytochemistry on human samples at 1:200. Biomaterials (2015) ncbi
mouse monoclonal (A3A12)
  • immunohistochemistry - frozen section; mouse
Abcam Lgals3 antibody (Abcam, ab2785) was used in immunohistochemistry - frozen section on mouse samples . J Mol Cell Cardiol (2015) ncbi
Santa Cruz Biotechnology
mouse monoclonal (B2C10)
  • western blot; mouse; 1:500; loading ...; fig s6b
Santa Cruz Biotechnology Lgals3 antibody (Santa Cruz, sc-32790) was used in western blot on mouse samples at 1:500 (fig s6b). Mol Neurodegener (2022) ncbi
rat monoclonal (M3/38)
  • immunohistochemistry - frozen section; mouse; 1:50; loading ...; fig s15b
  • immunocytochemistry; mouse; 1:50; loading ...; fig 2e
Santa Cruz Biotechnology Lgals3 antibody (Santa Cruz, sc-23938) was used in immunohistochemistry - frozen section on mouse samples at 1:50 (fig s15b) and in immunocytochemistry on mouse samples at 1:50 (fig 2e). Theranostics (2022) ncbi
mouse monoclonal (B2C10)
  • immunohistochemistry - paraffin section; mouse; 1:50; loading ...; fig 1g
  • immunohistochemistry - paraffin section; human; 1:50; loading ...; fig 1g
Santa Cruz Biotechnology Lgals3 antibody (Santa Cruz, sc-32790) was used in immunohistochemistry - paraffin section on mouse samples at 1:50 (fig 1g) and in immunohistochemistry - paraffin section on human samples at 1:50 (fig 1g). Nat Commun (2022) ncbi
rat monoclonal (M3/38)
  • immunocytochemistry; mouse; 1:250; loading ...; fig 2b
Santa Cruz Biotechnology Lgals3 antibody (Santa Cruz, sc-23938) was used in immunocytochemistry on mouse samples at 1:250 (fig 2b). Front Cell Dev Biol (2021) ncbi
rat monoclonal (M3/38)
  • immunocytochemistry; mouse; loading ...; fig 6b
In order to investigate how LC3-associated phagocytosis targets Legionella dumoffii to limit bacterial infection, Santa Cruz Biotechnology Lgals3 antibody (Santa Cruz, sc-23938) was used in immunocytochemistry on mouse samples (fig 6b). Sci Rep (2017) ncbi
rat monoclonal (M3/38)
  • immunohistochemistry - frozen section; mouse; 1:100; fig 2
In order to characterize microglia in the subventricular zone, Santa Cruz Biotechnology Lgals3 antibody (Santa Cruz, sc-23938) was used in immunohistochemistry - frozen section on mouse samples at 1:100 (fig 2). J Neuroinflammation (2016) ncbi
rat monoclonal (M3/38)
  • immunohistochemistry knockout validation; mouse; fig 7
  • western blot knockout validation; mouse; fig 5
  • immunohistochemistry - paraffin section; mouse; fig 7
Santa Cruz Biotechnology Lgals3 antibody (Santa Cruz, M3/38) was used in immunohistochemistry knockout validation on mouse samples (fig 7), in western blot knockout validation on mouse samples (fig 5) and in immunohistochemistry - paraffin section on mouse samples (fig 7). Kidney Int (2016) ncbi
rat monoclonal (M3/38)
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 2a
In order to use a mouse model to show that polyuria can induce hydronephrosis in the absence of anatomical or functional obstruction of the ureter, Santa Cruz Biotechnology Lgals3 antibody (Santa Cruz, sc-23938) was used in immunohistochemistry - paraffin section on mouse samples at 1:100 (fig 2a). Am J Physiol Renal Physiol (2016) ncbi
rat monoclonal (M3/38)
  • immunoprecipitation; human; fig 1
Santa Cruz Biotechnology Lgals3 antibody (Santa Cruz, M3/38) was used in immunoprecipitation on human samples (fig 1). BMC Cancer (2016) ncbi
mouse monoclonal (B2C10)
  • western blot; human; fig 3
Santa Cruz Biotechnology Lgals3 antibody (Santa Cruz, sc-32790) was used in western blot on human samples (fig 3). Sci Rep (2015) ncbi
mouse monoclonal (B2C10)
  • western blot; human; fig 1
Santa Cruz Biotechnology Lgals3 antibody (Santa Cruz, sc-32790) was used in western blot on human samples (fig 1). Glycobiology (2016) ncbi
rat monoclonal (M3/38)
  • immunohistochemistry - paraffin section; human; 1:200
Santa Cruz Biotechnology Lgals3 antibody (Santa Cruz, sc-23938) was used in immunohistochemistry - paraffin section on human samples at 1:200. Glia (2016) ncbi
mouse monoclonal (B2C10)
  • immunohistochemistry - paraffin section; human; 1:200
Santa Cruz Biotechnology Lgals3 antibody (Santa Cruz Biotechnology, sc-32790) was used in immunohistochemistry - paraffin section on human samples at 1:200. Thyroid (2015) ncbi
rat monoclonal (M3/38)
  • western blot; mouse; 1:5000; fig 4.e,f
Santa Cruz Biotechnology Lgals3 antibody (Santa Cruz, sc-23938) was used in western blot on mouse samples at 1:5000 (fig 4.e,f). Nat Commun (2015) ncbi
R&D Systems
domestic goat polyclonal
  • western blot; mouse; 1:200; loading ...; fig 6b
R&D Systems Lgals3 antibody (R&D Systems, AF1197) was used in western blot on mouse samples at 1:200 (fig 6b). Acta Neuropathol Commun (2021) ncbi
rat monoclonal (202213)
  • mass cytometry; mouse; loading ...; fig s3
R&D Systems Lgals3 antibody (R&D Systems, MAB1197) was used in mass cytometry on mouse samples (fig s3). EMBO J (2021) ncbi
domestic goat polyclonal
  • immunohistochemistry; mouse; loading ...
R&D Systems Lgals3 antibody (R&D Systems, AF1197) was used in immunohistochemistry on mouse samples . J Endocrinol (2021) ncbi
Invitrogen
mouse monoclonal (A3A12)
  • immunohistochemistry - paraffin section; human; 1:1000; loading ...; fig 1c, 4a
Invitrogen Lgals3 antibody (Thermo Fisher, A3A12) was used in immunohistochemistry - paraffin section on human samples at 1:1000 (fig 1c, 4a). PLoS ONE (2020) ncbi
mouse monoclonal (A3A12)
  • western blot; human; 1:2000
  • western blot; mouse; 1:2000
In order to find galectin-3(-/-) mice infected with Streptococcus pneumoniae develop severe pneumonia, Invitrogen Lgals3 antibody (Affinity Bioreagents, A3A12) was used in western blot on human samples at 1:2000 and in western blot on mouse samples at 1:2000. Am J Pathol (2008) ncbi
Articles Reviewed
  1. Tabel M, Wolf A, Szczepan M, Xu H, J xe4 gle H, Moehle C, et al. Genetic targeting or pharmacological inhibition of galectin-3 dampens microglia reactivity and delays retinal degeneration. J Neuroinflammation. 2022;19:229 pubmed publisher
  2. Chen Y, Xu J, Pan W, Xu X, Ma X, Chu Y, et al. Galectin-3 enhances trastuzumab resistance by regulating cancer malignancy and stemness in HER2-positive breast cancer cells. Thorac Cancer. 2022;13:1961-1973 pubmed publisher
  3. Liu W, Zhou H, Wang H, Zhang Q, Zhang R, Willard B, et al. IL-1R-IRAKM-Slc25a1 signaling axis reprograms lipogenesis in adipocytes to promote diet-induced obesity in mice. Nat Commun. 2022;13:2748 pubmed publisher
  4. Zhu J, Pittman S, Dhavale D, French R, Patterson J, Kaleelurrrahuman M, et al. VCP suppresses proteopathic seeding in neurons. Mol Neurodegener. 2022;17:30 pubmed publisher
  5. Maiseyeu A, Di L, Ravodina A, Barajas Espinosa A, Sakamoto A, Chaplin A, et al. Plaque-targeted, proteolysis-resistant, activatable and MRI-visible nano-GLP-1 receptor agonist targets smooth muscle cell differentiation in atherosclerosis. Theranostics. 2022;12:2741-2757 pubmed publisher
  6. Günes Günsel G, Conlon T, Jeridi A, Kim R, Ertuz Z, Lang N, et al. The arginine methyltransferase PRMT7 promotes extravasation of monocytes resulting in tissue injury in COPD. Nat Commun. 2022;13:1303 pubmed publisher
  7. Cheng C, Xue F, Sui W, Meng L, Xie L, Zhang C, et al. Deletion of natriuretic peptide receptor C alleviates adipose tissue inflammation in hypercholesterolemic Apolipoprotein E knockout mice. J Cell Mol Med. 2021;25:9837-9850 pubmed publisher
  8. Ribeiro T, Delgado García L, Porcionatto M. Notch1 and Galectin-3 Modulate Cortical Reactive Astrocyte Response After Brain Injury. Front Cell Dev Biol. 2021;9:649854 pubmed publisher
  9. Emre C, Do K, Jun B, Hjorth E, Alcalde S, Kautzmann M, et al. Age-related changes in brain phospholipids and bioactive lipids in the APP knock-in mouse model of Alzheimer's disease. Acta Neuropathol Commun. 2021;9:116 pubmed publisher
  10. Chen L, Cheng S, Sun K, Wang J, Liu X, Zhao Y, et al. Changes in macrophage and inflammatory cytokine expressions during fracture healing in an ovariectomized mice model. BMC Musculoskelet Disord. 2021;22:494 pubmed publisher
  11. Lagnado A, Leslie J, Ruchaud Sparagano M, Victorelli S, Hirsova P, Ogrodnik M, et al. Neutrophils induce paracrine telomere dysfunction and senescence in ROS-dependent manner. EMBO J. 2021;40:e106048 pubmed publisher
  12. Ruan L, Yao X, Li W, Zhang L, Yang H, Sun J, et al. Effect of galectin-3 in the pathogenesis of arteriovenous fistula stenosis formation. Ren Fail. 2021;43:566-576 pubmed publisher
  13. Li B, Yin J, Chang J, Zhang J, Wang Y, Huang H, et al. Apelin/APJ relieve diabetic cardiomyopathy by reducing microvascular dysfunction. J Endocrinol. 2021;249:1-18 pubmed publisher
  14. Varasteh Z, De Rose F, Mohanta S, Li Y, Zhang X, Miritsch B, et al. Imaging atherosclerotic plaques by targeting Galectin-3 and activated macrophages using (89Zr)-DFO- Galectin3-F(ab')2 mAb. Theranostics. 2021;11:1864-1876 pubmed publisher
  15. Yan W, Li T, Yin T, Hou Z, Qu K, Wang N, et al. M2 macrophage-derived exosomes promote the c-KIT phenotype of vascular smooth muscle cells during vascular tissue repair after intravascular stent implantation. Theranostics. 2020;10:10712-10728 pubmed publisher
  16. Song H, Xu T, Feng X, Lai Y, Yang Y, Zheng H, et al. Itaconate prevents abdominal aortic aneurysm formation through inhibiting inflammation via activation of Nrf2. EBioMedicine. 2020;57:102832 pubmed publisher
  17. Kasacka I, Piotrowska Z, Niezgoda M, Lewandowska A, Łebkowski W. Ageing-related changes in the levels of β-catenin, CacyBP/SIP, galectin-3 and immunoproteasome subunit LMP7 in the heart of men. PLoS ONE. 2020;15:e0229462 pubmed publisher
  18. Petrovic I, Pejnovic N, Ljujic B, Pavlovic S, Miletic Kovacevic M, Jeftic I, et al. Overexpression of Galectin 3 in Pancreatic β Cells Amplifies β-Cell Apoptosis and Islet Inflammation in Type-2 Diabetes in Mice. Front Endocrinol (Lausanne). 2020;11:30 pubmed publisher
  19. Yun Z, Wang Y, Feng W, Zang J, Zhang D, Gao Y. Overexpression of microRNA-185 alleviates intervertebral disc degeneration through inactivation of the Wnt/β-catenin signaling pathway and downregulation of Galectin-3. Mol Pain. 2020;16:1744806920902559 pubmed publisher
  20. Chen X, He Y, Xu A, Deng Z, Feng J, Lu F, et al. Increase of glandular epithelial cell clusters by an external volume expansion device promotes adipose tissue regeneration by recruiting macrophages. Biosci Rep. 2019;39: pubmed publisher
  21. Hubber A, Kubori T, Coban C, Matsuzawa T, Ogawa M, Kawabata T, et al. Bacterial secretion system skews the fate of Legionella-containing vacuoles towards LC3-associated phagocytosis. Sci Rep. 2017;7:44795 pubmed publisher
  22. Abshire C, Dragoi A, Roy C, Ivanov S. MTOR-Driven Metabolic Reprogramming Regulates Legionella pneumophila Intracellular Niche Homeostasis. PLoS Pathog. 2016;12:e1006088 pubmed publisher
  23. Hillis J, Davies J, Mundim M, Al Dalahmah O, Szele F. Cuprizone demyelination induces a unique inflammatory response in the subventricular zone. J Neuroinflammation. 2016;13:190 pubmed publisher
  24. Balbo B, Amaral A, Fonseca J, de Castro I, Salemi V, Souza L, et al. Cardiac dysfunction in Pkd1-deficient mice with phenotype rescue by galectin-3 knockout. Kidney Int. 2016;90:580-97 pubmed publisher
  25. Yoshioka W, Kawaguchi T, Nishimura N, Akagi T, Fujisawa N, Yanagisawa H, et al. Polyuria-associated hydronephrosis induced by xenobiotic chemical exposure in mice. Am J Physiol Renal Physiol. 2016;311:F752-F762 pubmed publisher
  26. Fritsch K, Mernberger M, Nist A, Stiewe T, Brehm A, Jacob R. Galectin-3 interacts with components of the nuclear ribonucleoprotein complex. BMC Cancer. 2016;16:502 pubmed publisher
  27. Weilner S, Keider V, Winter M, Harreither E, Salzer B, Weiss F, et al. Vesicular Galectin-3 levels decrease with donor age and contribute to the reduced osteo-inductive potential of human plasma derived extracellular vesicles. Aging (Albany NY). 2016;8:16-33 pubmed
  28. Tiwari A, Swamy S, Gopinath K, Kumar A. Genomic amplification upregulates estrogen-related receptor alpha and its depletion inhibits oral squamous cell carcinoma tumors in vivo. Sci Rep. 2015;5:17621 pubmed publisher
  29. Kuo H, Hsu H, Chen Y, Chang Y, Liu F, Wu C. Galectin-3 modulates the EGFR signalling-mediated regulation of Sox2 expression via c-Myc in lung cancer. Glycobiology. 2016;26:155-65 pubmed publisher
  30. James R, Hillis J, Adorján I, Gration B, Mundim M, Iqbal A, et al. Loss of galectin-3 decreases the number of immune cells in the subventricular zone and restores proliferation in a viral model of multiple sclerosis. Glia. 2016;64:105-21 pubmed publisher
  31. Ralhan R, Veyhl J, Chaker S, Assi J, Alyass A, Jeganathan A, et al. Immunohistochemical Subcellular Localization of Protein Biomarkers Distinguishes Benign from Malignant Thyroid Nodules: Potential for Fine-Needle Aspiration Biopsy Clinical Application. Thyroid. 2015;25:1224-34 pubmed publisher
  32. Abreu Vieira G, Fischer A, Mattsson C, de Jong J, Shabalina I, Ryden M, et al. Cidea improves the metabolic profile through expansion of adipose tissue. Nat Commun. 2015;6:7433 pubmed publisher
  33. Luo Z, Jiang L, Xu Y, Li H, Xu W, Wu S, et al. Mechano growth factor (MGF) and transforming growth factor (TGF)-β3 functionalized silk scaffolds enhance articular hyaline cartilage regeneration in rabbit model. Biomaterials. 2015;52:463-75 pubmed publisher
  34. Lin Y, Liu P, Adhikari N, Hall J, Wei L. RIP140 contributes to foam cell formation and atherosclerosis by regulating cholesterol homeostasis in macrophages. J Mol Cell Cardiol. 2015;79:287-94 pubmed publisher
  35. Farnworth S, Henderson N, Mackinnon A, Atkinson K, Wilkinson T, Dhaliwal K, et al. Galectin-3 reduces the severity of pneumococcal pneumonia by augmenting neutrophil function. Am J Pathol. 2008;172:395-405 pubmed publisher