This is a Validated Antibody Database (VAD) review about rat Lep, based on 3 published articles (read how Labome selects the articles), using Lep antibody in all methods. It is aimed to help Labome visitors find the most suited Lep antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Lep synonym: obese; leptin; obesity factor

Santa Cruz Biotechnology
mouse monoclonal (H-5)
  • western blot; mouse; loading ...; fig 10
Santa Cruz Biotechnology Lep antibody (SantaCruz, sc-393043) was used in western blot on mouse samples (fig 10). Am J Physiol Regul Integr Comp Physiol (2017) ncbi
Abcam
rabbit polyclonal
  • immunocytochemistry; human; 1:100; tbl 1
Abcam Lep antibody (Abcam, ab3583) was used in immunocytochemistry on human samples at 1:100 (tbl 1). Acta Biomater (2015) ncbi
Invitrogen
rabbit polyclonal
  • western blot; human; 1:400; loading ...; fig 2a
  • western blot; mouse; 1:400; loading ...; fig 5a
In order to propose that ER stress-induced CHOP activation in the brain links palmitate-induced regulation of leptin and IGF1, Invitrogen Lep antibody (ThermoFisher, PA1-051) was used in western blot on human samples at 1:400 (fig 2a) and in western blot on mouse samples at 1:400 (fig 5a). Cell Signal (2016) ncbi
Articles Reviewed
  1. Yi X, Gao H, Chen D, Tang D, Huang W, Li T, et al. Effects of obesity and exercise on testicular leptin signal transduction and testosterone biosynthesis in male mice. Am J Physiol Regul Integr Comp Physiol. 2017;312:R501-R510 pubmed publisher
  2. Marwarha G, Claycombe K, Schommer J, Collins D, Ghribi O. Palmitate-induced Endoplasmic Reticulum stress and subsequent C/EBP? Homologous Protein activation attenuates leptin and Insulin-like growth factor 1 expression in the brain. Cell Signal. 2016;28:1789-805 pubmed publisher
  3. Johnstone S, Liley M, Dalby M, Barnett S. Comparison of human olfactory and skeletal MSCs using osteogenic nanotopography to demonstrate bone-specific bioactivity of the surfaces. Acta Biomater. 2015;13:266-76 pubmed publisher