This is a Validated Antibody Database (VAD) review about rat Ldha, based on 15 published articles (read how Labome selects the articles), using Ldha antibody in all methods. It is aimed to help Labome visitors find the most suited Ldha antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Ldha synonym: CDK1; Ldh1

Knockout validation
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot knockout validation; human; loading ...; fig s3a
In order to study the activation of TAK1 by interleukin-1 and TRAF6, Cell Signaling Technology Ldha antibody (Cell Signaling, 2012) was used in western blot knockout validation on human samples (fig s3a). Biochem J (2017) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot knockout validation; mouse; loading ...; fig s1b
Cell Signaling Technology Ldha antibody (Cell Signaling, 2012S) was used in western blot knockout validation on mouse samples (fig s1b). Science (2016) ncbi
Abcam
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 1b
Abcam Ldha antibody (Abcam, ab47010) was used in western blot on human samples (fig 1b). Free Radic Biol Med (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 2d
Abcam Ldha antibody (Abcam, ab47010) was used in western blot on human samples at 1:1000 (fig 2d). Oncotarget (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 3a
Abcam Ldha antibody (Abcam, ab47010) was used in western blot on human samples (fig 3a). Oncotarget (2016) ncbi
Invitrogen
domestic rabbit polyclonal
  • other; human; 1:100; loading ...; fig 4a
In order to determine if cytosolic lactate is metabolized by the mitochondria of fermenting mammalian cells, Invitrogen Ldha antibody (Thermo Scientific, PA5-17183) was used in other on human samples at 1:100 (fig 4a). Nat Chem Biol (2016) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 4j
Cell Signaling Technology Ldha antibody (CST, 2012) was used in western blot on human samples (fig 4j). Sci Adv (2020) ncbi
domestic rabbit polyclonal
  • western blot knockout validation; human; loading ...; fig s3a
In order to study the activation of TAK1 by interleukin-1 and TRAF6, Cell Signaling Technology Ldha antibody (Cell Signaling, 2012) was used in western blot knockout validation on human samples (fig s3a). Biochem J (2017) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; human; 1:200; fig 7a
  • western blot; human; loading ...; fig 2a
In order to report that lactate dehydrogenase A phosphorylation results in pro-invasive, anti-anoikis, and pro-metastatic advantages to cancer cells, Cell Signaling Technology Ldha antibody (Cell Signaling Technology, 8176) was used in immunohistochemistry on human samples at 1:200 (fig 7a) and in western blot on human samples (fig 2a). Oncogene (2017) ncbi
domestic rabbit polyclonal
  • western blot knockout validation; mouse; loading ...; fig s1b
Cell Signaling Technology Ldha antibody (Cell Signaling, 2012S) was used in western blot knockout validation on mouse samples (fig s1b). Science (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:200; fig st1
In order to identify and characterize alterations in signal transduction that occur during the development Lapatinib resistance, Cell Signaling Technology Ldha antibody (Cell Signaling, 2012) was used in western blot on human samples at 1:200 (fig st1). Nat Commun (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; fig 3
In order to study the reprogramming of glucose metabolism in cancer cells by ROS-mediated stabilization of HIF1alpha via the immunoregulatory protein B7-H3, Cell Signaling Technology Ldha antibody (Cell Signaling Technology, 2012) was used in western blot on human samples (fig 3). Cancer Res (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; fig 4
In order to investigate protection of ovarian cancer-associated fibroblasts against oxidative stress by autophagy, Cell Signaling Technology Ldha antibody (Cell Signaling Technology, 2012) was used in western blot on human samples (fig 4). Cell Cycle (2016) ncbi
domestic rabbit monoclonal (C28H7)
  • immunohistochemistry - paraffin section; human; 1:250; fig 2
Cell Signaling Technology Ldha antibody (Cell Signaling, 3558) was used in immunohistochemistry - paraffin section on human samples at 1:250 (fig 2). PLoS ONE (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; fig 4
Cell Signaling Technology Ldha antibody (Cell Signaling Technology, 2012) was used in western blot on human samples (fig 4). Mol Cancer Res (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; fig 4g
  • western blot; mouse; 1:1000; fig 6l
Cell Signaling Technology Ldha antibody (Cell Signaling Technology, 2012) was used in western blot on human samples at 1:1000 (fig 4g) and in western blot on mouse samples at 1:1000 (fig 6l). FASEB J (2016) ncbi
domestic rabbit monoclonal (C28H7)
  • western blot; human; fig 1f
Cell Signaling Technology Ldha antibody (Cell Signaling Technology, 3558) was used in western blot on human samples (fig 1f). Oncogene (2015) ncbi
Articles Reviewed
  1. Aldonza M, Ku J, Hong J, Kim D, Yu S, Lee M, et al. Prior acquired resistance to paclitaxel relays diverse EGFR-targeted therapy persistence mechanisms. Sci Adv. 2020;6:eaav7416 pubmed publisher
  2. Zhang J, MacArtney T, Peggie M, Cohen P. Interleukin-1 and TRAF6-dependent activation of TAK1 in the absence of TAB2 and TAB3. Biochem J. 2017;474:2235-2248 pubmed publisher
  3. Jin L, Chun J, Pan C, Alesi G, Li D, Magliocca K, et al. Phosphorylation-mediated activation of LDHA promotes cancer cell invasion and tumour metastasis. Oncogene. 2017;36:3797-3806 pubmed publisher
  4. Zeng C, Wu Q, Wang J, Yao B, Ma L, Yang Z, et al. NOX4 supports glycolysis and promotes glutamine metabolism in non-small cell lung cancer cells. Free Radic Biol Med. 2016;101:236-248 pubmed publisher
  5. Li Y, Li X, Kan Q, Zhang M, Li X, Xu R, et al. Mitochondrial pyruvate carrier function is negatively linked to Warburg phenotype in vitro and malignant features in esophageal squamous cell carcinomas. Oncotarget. 2017;8:1058-1073 pubmed publisher
  6. Peng M, Yin N, Chhangawala S, Xu K, Leslie C, Li M. Aerobic glycolysis promotes T helper 1 cell differentiation through an epigenetic mechanism. Science. 2016;354:481-484 pubmed
  7. Treindl F, Ruprecht B, Beiter Y, Schultz S, Döttinger A, Staebler A, et al. A bead-based western for high-throughput cellular signal transduction analyses. Nat Commun. 2016;7:12852 pubmed publisher
  8. Chen Y, Mahieu N, Huang X, Singh M, Crawford P, Johnson S, et al. Lactate metabolism is associated with mammalian mitochondria. Nat Chem Biol. 2016;12:937-943 pubmed publisher
  9. Li Y, Li X, Li X, Zhong Y, Ji Y, Yu D, et al. PDHA1 gene knockout in prostate cancer cells results in metabolic reprogramming towards greater glutamine dependence. Oncotarget. 2016;7:53837-53852 pubmed publisher
  10. Lim S, Liu H, Madeira da Silva L, Arora R, Liu Z, Phillips J, et al. Immunoregulatory Protein B7-H3 Reprograms Glucose Metabolism in Cancer Cells by ROS-Mediated Stabilization of HIF1?. Cancer Res. 2016;76:2231-42 pubmed publisher
  11. Wang Q, Xue L, Zhang X, Bu S, Zhu X, Lai D. Autophagy protects ovarian cancer-associated fibroblasts against oxidative stress. Cell Cycle. 2016;15:1376-85 pubmed publisher
  12. Mohammad G, Olde Damink S, Malago M, Dhar D, Pereira S. Pyruvate Kinase M2 and Lactate Dehydrogenase A Are Overexpressed in Pancreatic Cancer and Correlate with Poor Outcome. PLoS ONE. 2016;11:e0151635 pubmed publisher
  13. Agarwal S, Bell C, Taylor S, Moran R. p53 Deletion or Hotspot Mutations Enhance mTORC1 Activity by Altering Lysosomal Dynamics of TSC2 and Rheb. Mol Cancer Res. 2016;14:66-77 pubmed publisher
  14. Sharma B, Kolhe R, Black S, Keller J, Mivechi N, Satyanarayana A. Inhibitor of differentiation 1 transcription factor promotes metabolic reprogramming in hepatocellular carcinoma cells. FASEB J. 2016;30:262-75 pubmed publisher
  15. Liu X, Yao J, Tripathi D, Ding Z, Xu Y, Sun M, et al. Autophagy mediates HIF2α degradation and suppresses renal tumorigenesis. Oncogene. 2015;34:2450-60 pubmed publisher