This is a Validated Antibody Database (VAD) review about rat LOC100360205, based on 6 published articles (read how Labome selects the articles), using LOC100360205 antibody in all methods. It is aimed to help Labome visitors find the most suited LOC100360205 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
LOC100360205 synonym: palladin

Proteintech Group
rabbit polyclonal
  • immunocytochemistry; rat; 1:100; loading ...; fig 4a
  • western blot; rat; 1:1000; loading ...; fig 3a
In order to explore how laminin2 in the basement membrane modulates the blood testis barrier dynamics during spermatogenesis, Proteintech Group LOC100360205 antibody (Proteintech, 10853-1-AP) was used in immunocytochemistry on rat samples at 1:100 (fig 4a) and in western blot on rat samples at 1:1000 (fig 3a). FASEB J (2017) ncbi
rabbit polyclonal
  • western blot; rat; 1:1000; loading ...; tbl 1
In order to examine the organization of actin microfilaments in Sertoli cell at the blood testis barrier, Proteintech Group LOC100360205 antibody (Proteintech, 10853-1-AP) was used in western blot on rat samples at 1:1000 (tbl 1). Spermatogenesis (2016) ncbi
rabbit polyclonal
  • immunoprecipitation; rat; fig 4
  • immunocytochemistry; rat; 1:100; fig 6
  • western blot; rat; 1:1000; fig 5
In order to report that crumbs homolog 3 is an actin microfilament regulator, Proteintech Group LOC100360205 antibody (Proteintech, 10853-1-AP) was used in immunoprecipitation on rat samples (fig 4), in immunocytochemistry on rat samples at 1:100 (fig 6) and in western blot on rat samples at 1:1000 (fig 5). Sci Rep (2016) ncbi
rabbit polyclonal
  • immunocytochemistry; rat; 1:100; fig 5
In order to characterize disruption of the sertolic cell cytoskeleton by interleukin 1alpha and its affect on gap junctional communication, Proteintech Group LOC100360205 antibody (Proteintech, 10853-I-AP) was used in immunocytochemistry on rat samples at 1:100 (fig 5). Cell Signal (2016) ncbi
rabbit polyclonal
  • immunohistochemistry; rat; 1:50
  • western blot; rat; 1:1000
In order to study the role of plastin 3 in ectoplasmic specialization dynamics during spermatogenesis in the rat testis, Proteintech Group LOC100360205 antibody (Proteintech, 10853-1-AP) was used in immunohistochemistry on rat samples at 1:50 and in western blot on rat samples at 1:1000. FASEB J (2015) ncbi
rabbit polyclonal
  • immunohistochemistry - frozen section; rat; 1:100
  • western blot; rat; 1:1000
In order to study the role of EB1 in tubulin and actin cytoskeletal networks at the sertoli cell blood-testis barrier, Proteintech Group LOC100360205 antibody (Protein Tech Group, 10853-1-AP) was used in immunohistochemistry - frozen section on rat samples at 1:100 and in western blot on rat samples at 1:1000. Endocrinology (2015) ncbi
Articles Reviewed
  1. Gao Y, Mruk D, Chen H, Lui W, Lee W, Cheng C. Regulation of the blood-testis barrier by a local axis in the testis: role of laminin ?2 in the basement membrane. FASEB J. 2017;31:584-597 pubmed publisher
  2. Li N, Lee W, Cheng C. Overexpression of plastin 3 in Sertoli cells disrupts actin microfilament bundle homeostasis and perturbs the tight junction barrier. Spermatogenesis. 2016;6:e1206353 pubmed publisher
  3. Gao Y, Lui W, Lee W, Cheng C. Polarity protein Crumbs homolog-3 (CRB3) regulates ectoplasmic specialization dynamics through its action on F-actin organization in Sertoli cells. Sci Rep. 2016;6:28589 pubmed publisher
  4. Chojnacka K, Bilinska B, Mruk D. Interleukin 1alpha-induced disruption of the Sertoli cell cytoskeleton affects gap junctional communication. Cell Signal. 2016;28:469-480 pubmed publisher
  5. Li N, Mruk D, Wong C, Lee W, Han D, Cheng C. Actin-bundling protein plastin 3 is a regulator of ectoplasmic specialization dynamics during spermatogenesis in the rat testis. FASEB J. 2015;29:3788-805 pubmed publisher
  6. Tang E, Mok K, Lee W, Cheng C. EB1 regulates tubulin and actin cytoskeletal networks at the sertoli cell blood-testis barrier in male rats: an in vitro study. Endocrinology. 2015;156:680-93 pubmed publisher