This is a Validated Antibody Database (VAD) review about rat Kcnma1, based on 9 published articles (read how Labome selects the articles), using Kcnma1 antibody in all methods. It is aimed to help Labome visitors find the most suited Kcnma1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Kcnma1 synonym: BKCa; KCNMA1b; KCNMA1c; Kcnma; Slo; cbv1; calcium-activated potassium channel subunit alpha-1; BK channel; BKCA alpha; KCa1.1; calcium-activated potassium channel alpha subunit; calcium-activated potassium channel, subfamily M subunit alpha-1; k(VCA)alpha; maxi K channel; maxiK; potassium channel, calcium activated large conductance subfamily M alpha, member 1; potassium large conductance calcium-activated channel, subfamily M, alpha member 1; slo homolog; slo-alpha; slo1; slowpoke homolog

Alomone Labs
rabbit polyclonal
  • western blot; human; 1:200; loading ...; fig 7g
In order to discover a large-conductance calcium-regulated potassium channel in the inner mitochondrial membrane of human dermal fibroblasts, Alomone Labs Kcnma1 antibody (Alomone Labs, APC-107) was used in western blot on human samples at 1:200 (fig 7g). Biochem J (2016) ncbi
rabbit polyclonal
  • western blot; human; 1:1000; fig 6b
In order to study the effects of hypertension and aging on the functional changes of large-conductance calcium- and voltage-activated potassium channels, Alomone Labs Kcnma1 antibody (Alomone Labs, APC-107) was used in western blot on human samples at 1:1000 (fig 6b). J Am Heart Assoc (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; 1:200; fig 5
In order to utilize the mechanosensitive TRPV4 channel in the collecting duct system of kidney that is coupled to expression of a diverse array of Ca2+-activated K+ channels (SK1/3, IK1, BK), Alomone Labs Kcnma1 antibody (Alomone, APC-107) was used in immunohistochemistry - frozen section on mouse samples at 1:200 (fig 5). PLoS ONE (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; 1:200; fig 5
In order to utilize the mechanosensitive TRPV4 channel in the collecting duct system of kidney that is coupled to expression of a diverse array of Ca2+-activated K+ channels (SK1/3, IK1, BK), Alomone Labs Kcnma1 antibody (Alomone, APC-151) was used in immunohistochemistry - frozen section on mouse samples at 1:200 (fig 5). PLoS ONE (2016) ncbi
rabbit polyclonal
  • immunocytochemistry; mouse; 1:100; fig 3
  • western blot; mouse; 1:1000; fig 1
In order to utilize the mechanosensitive TRPV4 channel in the collecting duct system of kidney that is coupled to expression of a diverse array of Ca2+-activated K+ channels (SK1/3, IK1, BK), Alomone Labs Kcnma1 antibody (Alomone, APC-021) was used in immunocytochemistry on mouse samples at 1:100 (fig 3) and in western blot on mouse samples at 1:1000 (fig 1). PLoS ONE (2016) ncbi
rabbit polyclonal
  • western blot; human; 1:500; fig 3
Alomone Labs Kcnma1 antibody (Alamone Labs, APC-107) was used in western blot on human samples at 1:500 (fig 3). Oncotarget (2016) ncbi
rabbit polyclonal
  • immunoprecipitation; mouse; fig 1
  • immunocytochemistry; human; 2 ug/ml; fig 2
In order to learn the connection between the MaxiK channel interactome and its interaction with GABA transporter 3 and heat shock protein 60 in the mammalian brain, Alomone Labs Kcnma1 antibody (Alomone Labs, APC021) was used in immunoprecipitation on mouse samples (fig 1) and in immunocytochemistry on human samples at 2 ug/ml (fig 2). Neuroscience (2016) ncbi
rabbit polyclonal
  • proximity ligation assay; rat; fig 4b
  • western blot; rat; fig 4a
Alomone Labs Kcnma1 antibody (Alomone Lab, APC-107) was used in proximity ligation assay on rat samples (fig 4b) and in western blot on rat samples (fig 4a). Physiol Rep (2016) ncbi
rabbit polyclonal
  • western blot; rat; fig 4
Alomone Labs Kcnma1 antibody (Alomone, APC-021) was used in western blot on rat samples (fig 4). Nat Neurosci (2015) ncbi
rabbit polyclonal
  • western blot; mouse; 1:100
In order to assess the specificity and sensitivity of six commercially available antibodies to BK beta1 using tissues from wild type and KO mice, Alomone Labs Kcnma1 antibody (Alomone Labs, APC-107) was used in western blot on mouse samples at 1:100. Physiol Rep (2014) ncbi
rabbit polyclonal
  • western blot; mouse; 1:200; loading ...; fig 3c
In order to test if AKAP150 mediates BKCa channel suppression during hyperglycemia and diabetes mellitus, Alomone Labs Kcnma1 antibody (Alomone, APC-021) was used in western blot on mouse samples at 1:200 (fig 3c). Circ Res (2014) ncbi
Articles Reviewed
  1. Kicinska A, Augustynek B, Kulawiak B, Jarmuszkiewicz W, Szewczyk A, Bednarczyk P. A large-conductance calcium-regulated K+ channel in human dermal fibroblast mitochondria. Biochem J. 2016;473:4457-4471 pubmed
  2. Cheng J, Mao L, Wen J, Li P, Wang N, Tan X, et al. Different Effects of Hypertension and Age on the Function of Large Conductance Calcium- and Voltage-Activated Potassium Channels in Human Mesentery Artery Smooth Muscle Cells. J Am Heart Assoc. 2016;5: pubmed publisher
  3. Li Y, Hu H, Butterworth M, Tian J, Zhu M, O Neil R. Expression of a Diverse Array of Ca2+-Activated K+ Channels (SK1/3, IK1, BK) that Functionally Couple to the Mechanosensitive TRPV4 Channel in the Collecting Duct System of Kidney. PLoS ONE. 2016;11:e0155006 pubmed publisher
  4. Edalat L, Stegen B, Klumpp L, Haehl E, Schilbach K, Lukowski R, et al. BK K+ channel blockade inhibits radiation-induced migration/brain infiltration of glioblastoma cells. Oncotarget. 2016;7:14259-78 pubmed publisher
  5. Singh H, Li M, Hall L, Chen S, Sukur S, Lu R, et al. MaxiK channel interactome reveals its interaction with GABA transporter 3 and heat shock protein 60 in the mammalian brain. Neuroscience. 2016;317:76-107 pubmed publisher
  6. Chen M, Li J, Jiang F, Fu J, Xia X, Du J, et al. Orai1 forms a signal complex with BKCa channel in mesenteric artery smooth muscle cells. Physiol Rep. 2016;4: pubmed publisher
  7. Laumet G, Garriga J, Chen S, Zhang Y, Li D, Smith T, et al. G9a is essential for epigenetic silencing of K(+) channel genes in acute-to-chronic pain transition. Nat Neurosci. 2015;18:1746-55 pubmed publisher
  8. Bhattarai Y, Fernandes R, Kadrofske M, Lockwood L, Galligan J, Xu H. Western blot analysis of BK channel β1-subunit expression should be interpreted cautiously when using commercially available antibodies. Physiol Rep. 2014;2: pubmed publisher
  9. Nystoriak M, Nieves Cintrón M, Nygren P, Hinke S, Nichols C, Chen C, et al. AKAP150 contributes to enhanced vascular tone by facilitating large-conductance Ca2+-activated K+ channel remodeling in hyperglycemia and diabetes mellitus. Circ Res. 2014;114:607-15 pubmed publisher