This is a Validated Antibody Database (VAD) review about rat Itpr3, based on 18 published articles (read how Labome selects the articles), using Itpr3 antibody in all methods. It is aimed to help Labome visitors find the most suited Itpr3 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Itpr3 synonym: IP3R-3; IP3R3; IP3R3X

Knockout validation
BD Biosciences
mouse monoclonal (2/IP3R-3)
  • western blot knockout validation; human; 1:1000; loading ...; fig s5a
BD Biosciences Itpr3 antibody (BD, 610312) was used in western blot knockout validation on human samples at 1:1000 (fig s5a). Cell Rep (2018) ncbi
BD Biosciences
mouse monoclonal (2/IP3R-3)
  • western blot knockout validation; mouse; loading ...; fig 1d
In order to elucidate the role of IP3R in regulating blood pressure in vivo, BD Biosciences Itpr3 antibody (BD Biosciences, 610312) was used in western blot knockout validation on mouse samples (fig 1d). JCI Insight (2016) ncbi
Santa Cruz Biotechnology
mouse monoclonal (B-2)
  • western blot; human; 1:200; fig 1
Santa Cruz Biotechnology Itpr3 antibody (Santa Cruz Biotechnology, sc-377518) was used in western blot on human samples at 1:200 (fig 1). Stem Cells Dev (2016) ncbi
BD Biosciences
mouse monoclonal (2/IP3R-3)
  • western blot; human; 1:1000; loading ...; fig 4a, 4d, 4g
BD Biosciences Itpr3 antibody (BD Pharmingen, 610312) was used in western blot on human samples at 1:1000 (fig 4a, 4d, 4g). Nat Commun (2021) ncbi
mouse monoclonal (2/IP3R-3)
  • immunocytochemistry; human; 1:200; loading ...; fig s4e
  • western blot; human; 1:1000; loading ...; fig s5h
BD Biosciences Itpr3 antibody (BD Transduction, 610313) was used in immunocytochemistry on human samples at 1:200 (fig s4e) and in western blot on human samples at 1:1000 (fig s5h). Nat Commun (2021) ncbi
mouse monoclonal (2/IP3R-3)
  • immunohistochemistry - frozen section; mouse; 1:300; loading ...; fig 4d
BD Biosciences Itpr3 antibody (BDTransduction Laboratories, 610312) was used in immunohistochemistry - frozen section on mouse samples at 1:300 (fig 4d). Proc Natl Acad Sci U S A (2019) ncbi
mouse monoclonal (2/IP3R-3)
  • western blot; mouse; loading ...; fig 4a
BD Biosciences Itpr3 antibody (BD Biosciences, 610312) was used in western blot on mouse samples (fig 4a). Cell Rep (2018) ncbi
mouse monoclonal (2/IP3R-3)
  • western blot knockout validation; human; 1:1000; loading ...; fig s5a
BD Biosciences Itpr3 antibody (BD, 610312) was used in western blot knockout validation on human samples at 1:1000 (fig s5a). Cell Rep (2018) ncbi
mouse monoclonal (2/IP3R-3)
  • immunohistochemistry; mouse; 1:50; loading ...; fig s2i
BD Biosciences Itpr3 antibody (BD Biosciences, 610313) was used in immunohistochemistry on mouse samples at 1:50 (fig s2i). Proc Natl Acad Sci U S A (2018) ncbi
mouse monoclonal (2/IP3R-3)
  • western blot; mouse; loading ...; fig 3a
BD Biosciences Itpr3 antibody (BD Biosciences, 610312) was used in western blot on mouse samples (fig 3a). Mol Cell (2017) ncbi
mouse monoclonal (2/IP3R-3)
  • western blot; human; loading ...; fig 2a
BD Biosciences Itpr3 antibody (BD Biosciences, 610312) was used in western blot on human samples (fig 2a). Nature (2017) ncbi
mouse monoclonal (2/IP3R-3)
  • immunohistochemistry; human; loading ...; fig s6k
  • western blot; human; loading ...; fig 1c
In order to understand the interaction between PTEN and FBXL2 in the context of apoptosis, BD Biosciences Itpr3 antibody (BD-Pharmingen, 610312) was used in immunohistochemistry on human samples (fig s6k) and in western blot on human samples (fig 1c). Nature (2017) ncbi
mouse monoclonal (2/IP3R-3)
  • western blot; human; 1:1000; loading ...; fig 2f
  • immunohistochemistry; mouse; 1:1000; loading ...; fig 6a
  • western blot; mouse; 1:1000; loading ...; fig 5e
BD Biosciences Itpr3 antibody (BD biosciences, 610313) was used in western blot on human samples at 1:1000 (fig 2f), in immunohistochemistry on mouse samples at 1:1000 (fig 6a) and in western blot on mouse samples at 1:1000 (fig 5e). EMBO Mol Med (2016) ncbi
mouse monoclonal (2/IP3R-3)
  • western blot knockout validation; mouse; loading ...; fig 1d
In order to elucidate the role of IP3R in regulating blood pressure in vivo, BD Biosciences Itpr3 antibody (BD Biosciences, 610312) was used in western blot knockout validation on mouse samples (fig 1d). JCI Insight (2016) ncbi
mouse monoclonal (2/IP3R-3)
  • western blot; human; fig 4
BD Biosciences Itpr3 antibody (BD, 610312) was used in western blot on human samples (fig 4). Oncotarget (2015) ncbi
mouse monoclonal (2/IP3R-3)
  • western blot; human; fig 4
BD Biosciences Itpr3 antibody (BD Biosciences, 610312) was used in western blot on human samples (fig 4). Int J Mol Sci (2015) ncbi
mouse monoclonal (2/IP3R-3)
  • western blot; human; fig 2
BD Biosciences Itpr3 antibody (BD Biosciences, 610312) was used in western blot on human samples (fig 2). PLoS ONE (2015) ncbi
mouse monoclonal (2/IP3R-3)
  • immunohistochemistry; mouse; 1:500
BD Biosciences Itpr3 antibody (BD Pharmingen, 610312) was used in immunohistochemistry on mouse samples at 1:500. J Comp Neurol (2015) ncbi
mouse monoclonal (2/IP3R-3)
  • immunohistochemistry; mouse; 1:500
In order to identify a method for simultaneous use of both biochemistry and immunohistochemistry in regards to the same tissue, BD Biosciences Itpr3 antibody (BD BioScience, 610312) was used in immunohistochemistry on mouse samples at 1:500. Eur J Neurosci (2014) ncbi
mouse monoclonal (2/IP3R-3)
  • immunocytochemistry; human
BD Biosciences Itpr3 antibody (BD Transduction Laboratories, 610313) was used in immunocytochemistry on human samples . Biochem Biophys Res Commun (2013) ncbi
Articles Reviewed
  1. Xue Y, Morris J, Yang K, Fu Z, Zhu X, Johnson F, et al. SMARCA4/2 loss inhibits chemotherapy-induced apoptosis by restricting IP3R3-mediated Ca2+ flux to mitochondria. Nat Commun. 2021;12:5404 pubmed publisher
  2. Thillaiappan N, Smith H, Atakpa Adaji P, Taylor C. KRAP tethers IP3 receptors to actin and licenses them to evoke cytosolic Ca2+ signals. Nat Commun. 2021;12:4514 pubmed publisher
  3. Luo X, Chen Z, Xue J, Zhao D, Lu C, Li Y, et al. Infection by the parasitic helminth Trichinella spiralis activates a Tas2r-mediated signaling pathway in intestinal tuft cells. Proc Natl Acad Sci U S A. 2019;116:5564-5569 pubmed publisher
  4. D Eletto M, Rossin F, Occhigrossi L, Farrace M, Faccenda D, Desai R, et al. Transglutaminase Type 2 Regulates ER-Mitochondria Contact Sites by Interacting with GRP75. Cell Rep. 2018;25:3573-3581.e4 pubmed publisher
  5. Atakpa P, Thillaiappan N, Mataragka S, Prole D, Taylor C. IP3 Receptors Preferentially Associate with ER-Lysosome Contact Sites and Selectively Deliver Ca2+ to Lysosomes. Cell Rep. 2018;25:3180-3193.e7 pubmed publisher
  6. Dutta Banik D, Martin L, Freichel M, Torregrossa A, Medler K. TRPM4 and TRPM5 are both required for normal signaling in taste receptor cells. Proc Natl Acad Sci U S A. 2018;115:E772-E781 pubmed publisher
  7. Zhao Y, Chen Y, Miao G, Zhao H, Qu W, Li D, et al. The ER-Localized Transmembrane Protein EPG-3/VMP1 Regulates SERCA Activity to Control ER-Isolation Membrane Contacts for Autophagosome Formation. Mol Cell. 2017;67:974-989.e6 pubmed publisher
  8. Bononi A, Giorgi C, Patergnani S, Larson D, Verbruggen K, Tanji M, et al. BAP1 regulates IP3R3-mediated Ca2+ flux to mitochondria suppressing cell transformation. Nature. 2017;546:549-553 pubmed publisher
  9. Kuchay S, Giorgi C, Simoneschi D, Pagan J, Missiroli S, Saraf A, et al. PTEN counteracts FBXL2 to promote IP3R3- and Ca2+-mediated apoptosis limiting tumour growth. Nature. 2017;546:554-558 pubmed publisher
  10. Watanabe S, Ilieva H, Tamada H, Nomura H, Komine O, Endo F, et al. Mitochondria-associated membrane collapse is a common pathomechanism in SIGMAR1- and SOD1-linked ALS. EMBO Mol Med. 2016;8:1421-1437 pubmed publisher
  11. Lin Q, Zhao G, Fang X, Peng X, Tang H, Wang H, et al. IP3 receptors regulate vascular smooth muscle contractility and hypertension. JCI Insight. 2016;1:e89402 pubmed publisher
  12. Zuccolo E, Bottino C, Diofano F, Poletto V, Codazzi A, Mannarino S, et al. Constitutive Store-Operated Ca(2+) Entry Leads to Enhanced Nitric Oxide Production and Proliferation in Infantile Hemangioma-Derived Endothelial Colony-Forming Cells. Stem Cells Dev. 2016;25:301-19 pubmed publisher
  13. Patergnani S, Giorgi C, Maniero S, Missiroli S, Maniscalco P, Bononi I, et al. The endoplasmic reticulum mitochondrial calcium cross talk is downregulated in malignant pleural mesothelioma cells and plays a critical role in apoptosis inhibition. Oncotarget. 2015;6:23427-44 pubmed
  14. Macvicar T, Mannack L, Lees R, Lane J. Targeted siRNA Screens Identify ER-to-Mitochondrial Calcium Exchange in Autophagy and Mitophagy Responses in RPE1 Cells. Int J Mol Sci. 2015;16:13356-80 pubmed publisher
  15. Shambharkar P, Bittinger M, Latario B, Xiong Z, Bandyopadhyay S, Davis V, et al. TMEM203 Is a Novel Regulator of Intracellular Calcium Homeostasis and Is Required for Spermatogenesis. PLoS ONE. 2015;10:e0127480 pubmed publisher
  16. PFISTER S, Weber T, Härtig W, Schwerdel C, Elsaesser R, Knuesel I, et al. Novel role of cystic fibrosis transmembrane conductance regulator in maintaining adult mouse olfactory neuronal homeostasis. J Comp Neurol. 2015;523:406-30 pubmed publisher
  17. Notter T, Panzanelli P, PFISTER S, Mircsof D, Fritschy J. A protocol for concurrent high-quality immunohistochemical and biochemical analyses in adult mouse central nervous system. Eur J Neurosci. 2014;39:165-75 pubmed publisher
  18. Matsuzaki H, Fujimoto T, Tanaka M, Shirasawa S. Tespa1 is a novel component of mitochondria-associated endoplasmic reticulum membranes and affects mitochondrial calcium flux. Biochem Biophys Res Commun. 2013;433:322-6 pubmed publisher