This is a Validated Antibody Database (VAD) review about rat Itch, based on 15 published articles (read how Labome selects the articles), using Itch antibody in all methods. It is aimed to help Labome visitors find the most suited Itch antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Itch synonym: E3 ubiquitin-protein ligase Itchy homolog; itch E3 ubiquitin ligase; itchy E3 ubiquitin protein ligase homolog

Knockout validation
BD Biosciences
mouse monoclonal (32/Itch)
  • western blot knockout validation; mouse; 1:1000; fig 5
  • immunoprecipitation; mouse; fig 3
In order to analyze inhibition of IL-17-mediated colon inflammation and tumorigenesis by ROR-gamma-t ubiquitination by Itch, BD Biosciences Itch antibody (BD, 32) was used in western blot knockout validation on mouse samples at 1:1000 (fig 5) and in immunoprecipitation on mouse samples (fig 3). Nat Immunol (2016) ncbi
BD Biosciences
mouse monoclonal (32/Itch)
  • western blot knockout validation; mouse; fig 5
In order to report an Itch-Cyld-mediated regulatory mechanism in macrophages, BD Biosciences Itch antibody (BD, 611198) was used in western blot knockout validation on mouse samples (fig 5). Nat Immunol (2011) ncbi
Cell Signaling Technology
rabbit monoclonal (D8Q6D)
  • western blot; human; loading ...; fig 2a
In order to report that DUB3 family deubiquitylating enzymes regulate Hippo pathway activity, Cell Signaling Technology Itch antibody (Cell Signaling, 12117) was used in western blot on human samples (fig 2a). PLoS ONE (2017) ncbi
rabbit monoclonal (D8Q6D)
  • western blot; human; fig 4
In order to analyze AMOTL1 and how it is antagonized by Merlin and promotes breast cancer progression, Cell Signaling Technology Itch antibody (Cell Signaling, 12117) was used in western blot on human samples (fig 4). Neoplasia (2016) ncbi
rabbit monoclonal (D8Q6D)
  • western blot; mouse; fig 9
In order to study the function of the PHD motif of MEKK1, Cell Signaling Technology Itch antibody (Cell Signaling, 12117) was used in western blot on mouse samples (fig 9). EMBO J (2014) ncbi
BD Biosciences
mouse monoclonal (32/Itch)
  • western blot; mouse; loading ...; fig 8b
BD Biosciences Itch antibody (BD Biosciences, 611198) was used in western blot on mouse samples (fig 8b). J Exp Med (2018) ncbi
mouse monoclonal (32/Itch)
  • western blot; mouse; loading ...; fig s3a
In order to study NaV1.7 trafficking, BD Biosciences Itch antibody (BD Biosciences, 611198) was used in western blot on mouse samples (fig s3a). Proc Natl Acad Sci U S A (2016) ncbi
mouse monoclonal (32/Itch)
  • western blot; human; loading ...; fig 1b
In order to elucidate how Cdh1 regulates NEDD4 family E3 ligases such as WWP2, BD Biosciences Itch antibody (BD Labs, 611198) was used in western blot on human samples (fig 1b). Cell Discov (2016) ncbi
mouse monoclonal (32/Itch)
  • western blot knockout validation; mouse; 1:1000; fig 5
  • immunoprecipitation; mouse; fig 3
In order to analyze inhibition of IL-17-mediated colon inflammation and tumorigenesis by ROR-gamma-t ubiquitination by Itch, BD Biosciences Itch antibody (BD, 32) was used in western blot knockout validation on mouse samples at 1:1000 (fig 5) and in immunoprecipitation on mouse samples (fig 3). Nat Immunol (2016) ncbi
mouse monoclonal (32/Itch)
  • western blot; mouse; 1:500; loading ...; fig 6a
In order to demonstrate that Ndfip1/Ndfip2 regulate cross talk between T-cell receptor and cytokine signaling pathways, BD Biosciences Itch antibody (BD Biosciences, 32) was used in western blot on mouse samples at 1:500 (fig 6a). Nat Commun (2016) ncbi
mouse monoclonal (32/Itch)
  • other; human; loading ...; fig st1
  • western blot; human; loading ...; fig st4
In order to use size exclusion chromatography-microsphere-based affinity proteomics to study clinical samples obtained from pediatric acute leukemia patients, BD Biosciences Itch antibody (BD, 32) was used in other on human samples (fig st1) and in western blot on human samples (fig st4). Mol Cell Proteomics (2016) ncbi
mouse monoclonal (32/Itch)
  • western blot; human
BD Biosciences Itch antibody (BD pharmigen, 611198) was used in western blot on human samples . Sci Rep (2015) ncbi
mouse monoclonal (32/Itch)
  • immunoprecipitation; human; fig 3
  • western blot; mouse; fig 3
In order to provide mechanistic insights into how Itch function is regulated during inflammatory signaling, BD Biosciences Itch antibody (BD, 611198) was used in immunoprecipitation on human samples (fig 3) and in western blot on mouse samples (fig 3). J Immunol (2015) ncbi
mouse monoclonal (32/Itch)
  • western blot; hamsters; fig 5
In order to determine the localization of host proteins within vesicular stomatitis virus virions, BD Biosciences Itch antibody (BD, 32/Itch) was used in western blot on hamsters samples (fig 5). PLoS ONE (2014) ncbi
mouse monoclonal (32/Itch)
  • immunohistochemistry; human
BD Biosciences Itch antibody (BD Transduction Laboratories, 611198) was used in immunohistochemistry on human samples . Neurobiol Dis (2013) ncbi
mouse monoclonal (32/Itch)
  • western blot; chicken
  • western blot; human
BD Biosciences Itch antibody (BD Biosciences, 611198) was used in western blot on chicken samples and in western blot on human samples . Mol Cell Biol (2013) ncbi
mouse monoclonal (32/Itch)
  • western blot knockout validation; mouse; fig 5
In order to report an Itch-Cyld-mediated regulatory mechanism in macrophages, BD Biosciences Itch antibody (BD, 611198) was used in western blot knockout validation on mouse samples (fig 5). Nat Immunol (2011) ncbi
Articles Reviewed
  1. Chen S, Yun F, Yao Y, Cao M, Zhang Y, Wang J, et al. USP38 critically promotes asthmatic pathogenesis by stabilizing JunB protein. J Exp Med. 2018;215:2850-2867 pubmed publisher
  2. Nguyen H, Kugler J, Cohen S. DUB3 Deubiquitylating Enzymes Regulate Hippo Pathway Activity by Regulating the Stability of ITCH, LATS and AMOT Proteins. PLoS ONE. 2017;12:e0169587 pubmed publisher
  3. Dustrude E, Moutal A, Yang X, Wang Y, Khanna M, Khanna R. Hierarchical CRMP2 posttranslational modifications control NaV1.7 function. Proc Natl Acad Sci U S A. 2016;113:E8443-E8452 pubmed publisher
  4. Liu J, Wan L, Liu J, Yuan Z, Zhang J, Guo J, et al. Cdh1 inhibits WWP2-mediated ubiquitination of PTEN to suppress tumorigenesis in an APC-independent manner. Cell Discov. 2016;2:15044 pubmed publisher
  5. Kathania M, Khare P, Zeng M, Cantarel B, Zhang H, Ueno H, et al. Itch inhibits IL-17-mediated colon inflammation and tumorigenesis by ROR-?t ubiquitination. Nat Immunol. 2016;17:997-1004 pubmed publisher
  6. O Leary C, Riling C, Spruce L, Ding H, Kumar S, Deng G, et al. Ndfip-mediated degradation of Jak1 tunes cytokine signalling to limit expansion of CD4+ effector T cells. Nat Commun. 2016;7:11226 pubmed publisher
  7. Couderc C, Boin A, Fuhrmann L, Vincent Salomon A, Mandati V, Kieffer Y, et al. AMOTL1 Promotes Breast Cancer Progression and Is Antagonized by Merlin. Neoplasia. 2016;18:10-24 pubmed publisher
  8. Kanderová V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, et al. High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016;15:1246-61 pubmed publisher
  9. Stöhr R, Mavilio M, Marino A, Casagrande V, Kappel B, Möllmann J, et al. ITCH modulates SIRT6 and SREBP2 to influence lipid metabolism and atherosclerosis in ApoE null mice. Sci Rep. 2015;5:9023 pubmed publisher
  10. Kathania M, Zeng M, Yadav V, Moghaddam S, Yang B, Venuprasad K. Ndfip1 regulates itch ligase activity and airway inflammation via UbcH7. J Immunol. 2015;194:2160-7 pubmed publisher
  11. Charlaftis N, Suddason T, Wu X, Anwar S, Karin M, Gallagher E. The MEKK1 PHD ubiquitinates TAB1 to activate MAPKs in response to cytokines. EMBO J. 2014;33:2581-96 pubmed publisher
  12. Moerdyk Schauwecker M, Hwang S, Grdzelishvili V. Cellular proteins associated with the interior and exterior of vesicular stomatitis virus virions. PLoS ONE. 2014;9:e104688 pubmed publisher
  13. Pranski E, Dalal N, Sanford C, Herskowitz J, Gearing M, Lazo C, et al. RING finger protein 11 (RNF11) modulates susceptibility to 6-OHDA-induced nigral degeneration and behavioral deficits through NF-?B signaling in dopaminergic cells. Neurobiol Dis. 2013;54:264-79 pubmed publisher
  14. Moreno Garcia M, Sommer K, Rincón Arano H, Brault M, Ninomiya Tsuji J, Matesic L, et al. Kinase-independent feedback of the TAK1/TAB1 complex on BCL10 turnover and NF-?B activation. Mol Cell Biol. 2013;33:1149-63 pubmed publisher
  15. Ahmed N, Zeng M, Sinha I, Polin L, Wei W, Rathinam C, et al. The E3 ligase Itch and deubiquitinase Cyld act together to regulate Tak1 and inflammation. Nat Immunol. 2011;12:1176-83 pubmed publisher