This is a Validated Antibody Database (VAD) review about rat Il10, based on 13 published articles (read how Labome selects the articles), using Il10 antibody in all methods. It is aimed to help Labome visitors find the most suited Il10 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Il10 synonym: IL10X; interleukin-10; CSIF; IL-10; cytokine synthesis inhibitory factor

Abcam
rabbit polyclonal
  • western blot; rat; loading ...; fig 6a
In order to assess the critical role of programmed death protein 1/programmed death-ligand 1 in the polarization of microglia during intracerebral hemorrhage-induced secondary brain injury, Abcam Il10 antibody (Abcam, ab9969) was used in western blot on rat samples (fig 6a). J Neuroinflammation (2017) ncbi
rat monoclonal (JES5-2A5)
  • western blot; mouse; 1:500; fig 4a
In order to determine the contribution of lymphatic drainage to tumor inflammation and immunity, Abcam Il10 antibody (Abcam, ab33471) was used in western blot on mouse samples at 1:500 (fig 4a). J Clin Invest (2016) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse; 1:100; fig 4
Abcam Il10 antibody (abcam, ab9969) was used in immunohistochemistry on mouse samples at 1:100 (fig 4). J Neuroinflammation (2016) ncbi
rat monoclonal (JES5-2A5)
  • immunohistochemistry - frozen section; mouse; fig 3c
Abcam Il10 antibody (Abcam, ab33471) was used in immunohistochemistry - frozen section on mouse samples (fig 3c). Cell Mol Immunol (2016) ncbi
rabbit polyclonal
  • western blot; rat; 1:500
Abcam Il10 antibody (Abcam, ab9969) was used in western blot on rat samples at 1:500. PLoS ONE (2015) ncbi
rabbit polyclonal
  • blocking or activating experiments; rat
  • western blot; rat; 1:1,000
Abcam Il10 antibody (Abcam, ab9969) was used in blocking or activating experiments on rat samples and in western blot on rat samples at 1:1,000. Inflammopharmacology (2014) ncbi
R&D Systems
goat polyclonal
  • immunohistochemistry; mouse; 1:50; loading ...; fig 5h
R&D Systems Il10 antibody (R&D, AF519) was used in immunohistochemistry on mouse samples at 1:50 (fig 5h). Nat Commun (2019) ncbi
goat polyclonal
  • immunohistochemistry; mouse; 1:20; fig 2
In order to study how mitigation of Alzheimer's disease pathology occurs by breaking immune tolerance by targeting Foxp3(+) regulatory T cells, R&D Systems Il10 antibody (R&D systems, AF519) was used in immunohistochemistry on mouse samples at 1:20 (fig 2). Nat Commun (2015) ncbi
goat polyclonal
  • immunohistochemistry - frozen section; mouse; 1:100; fig 6(a-c)
R&D Systems Il10 antibody (R&D systems, AF519) was used in immunohistochemistry - frozen section on mouse samples at 1:100 (fig 6(a-c)). Brain (2015) ncbi
goat polyclonal
  • ELISA; human
R&D Systems Il10 antibody (R&D Systems, (AF519) was used in ELISA on human samples . J Immunol Methods (2015) ncbi
Invitrogen
rabbit polyclonal
  • immunohistochemistry; rat; 1:100
In order to investigate the effects of prior treatment of simvastatin on mitochondrial enzyme, ghrelin, and hypoxia-inducible factor 1 alpha in septic rats, Invitrogen Il10 antibody (Invitrogen, ARC0102) was used in immunohistochemistry on rat samples at 1:100. Int J Clin Exp Med (2015) ncbi
mouse monoclonal (2G101H7)
  • western blot; rat; fig 6
In order to assess the role of trolox in chronic bile duct ligation, Invitrogen Il10 antibody (Invitrogen, ARC9102) was used in western blot on rat samples (fig 6). Fundam Clin Pharmacol (2013) ncbi
BD Biosciences
mouse monoclonal (A5-4)
  • flow cytometry; rat; loading ...; fig 5a
In order to use rats to study cellular and molecular mechanisms underlying specific sexual dimorphism in T lymphocyte-dependent inflammatory responses, BD Biosciences Il10 antibody (BD Biosciences, A5-4) was used in flow cytometry on rat samples (fig 5a). Brain Behav Immun (2015) ncbi
Articles Reviewed
  1. Rosenzweig N, Dvir Szternfeld R, Tsitsou Kampeli A, Keren Shaul H, Ben Yehuda H, Weill Raynal P, et al. PD-1/PD-L1 checkpoint blockade harnesses monocyte-derived macrophages to combat cognitive impairment in a tauopathy mouse model. Nat Commun. 2019;10:465 pubmed publisher
  2. Wu J, Sun L, Li H, Shen H, Zhai W, Yu Z, et al. Roles of programmed death protein 1/programmed death-ligand 1 in secondary brain injury after intracerebral hemorrhage in rats: selective modulation of microglia polarization to anti-inflammatory phenotype. J Neuroinflammation. 2017;14:36 pubmed publisher
  3. Lund A, Wagner M, Fankhauser M, Steinskog E, Broggi M, Spranger S, et al. Lymphatic vessels regulate immune microenvironments in human and murine melanoma. J Clin Invest. 2016;126:3389-402 pubmed publisher
  4. Lee K, Lee H, Lin H, Tsay H, Tsai F, Shyue S, et al. Role of transient receptor potential ankyrin 1 channels in Alzheimer's disease. J Neuroinflammation. 2016;13:92 pubmed publisher
  5. Baruch K, Rosenzweig N, Kertser A, Deczkowska A, Sharif A, Spinrad A, et al. Breaking immune tolerance by targeting Foxp3(+) regulatory T cells mitigates Alzheimer's disease pathology. Nat Commun. 2015;6:7967 pubmed publisher
  6. Jiang Y, Gao Q, Wang L, Guo C, Zhu F, Wang B, et al. Deficiency of programmed cell death 4 results in increased IL-10 expression by macrophages and thereby attenuates atherosclerosis in hyperlipidemic mice. Cell Mol Immunol. 2016;13:524-34 pubmed publisher
  7. Yorulmaz H, Ozkok E, Erguven M, Ates G, Aydın I, Tamer S. Effect of simvastatin on mitochondrial enzyme activities, ghrelin, hypoxia-inducible factor 1α in hepatic tissue during early phase of sepsis. Int J Clin Exp Med. 2015;8:3640-50 pubmed
  8. Koronyo Y, Salumbides B, Sheyn J, Pelissier L, Li S, Ljubimov V, et al. Therapeutic effects of glatiramer acetate and grafted CD115⁺ monocytes in a mouse model of Alzheimer's disease. Brain. 2015;138:2399-422 pubmed publisher
  9. Nacka Aleksić M, Djikić J, Pilipović I, Stojić Vukanić Z, Kosec D, Bufan B, et al. Male rats develop more severe experimental autoimmune encephalomyelitis than female rats: sexual dimorphism and diergism at the spinal cord level. Brain Behav Immun. 2015;49:101-18 pubmed publisher
  10. Guo S, Jin Y, Fang Q, You C, Wang X, Hu X, et al. Beneficial effects of hydrogen-rich saline on early burn-wound progression in rats. PLoS ONE. 2015;10:e0124897 pubmed publisher
  11. Potas J, Haque F, Maclean F, Nisbet D. Interleukin-10 conjugated electrospun polycaprolactone (PCL) nanofibre scaffolds for promoting alternatively activated (M2) macrophages around the peripheral nerve in vivo. J Immunol Methods. 2015;420:38-49 pubmed publisher
  12. Zaringhalam J, Akhtari Z, Eidi A, Ruhani A, Tekieh E. Relationship between serum IL10 level and p38MAPK enzyme activity on behavioral and cellular aspects of variation of hyperalgesia during different stages of arthritis in rats. Inflammopharmacology. 2014;22:37-44 pubmed publisher
  13. Galicia Moreno M, Favari L, Muriel P. Trolox mitigates fibrosis in a bile duct ligation model. Fundam Clin Pharmacol. 2013;27:308-18 pubmed publisher