This is a Validated Antibody Database (VAD) review about rat Ifng, based on 36 published articles (read how Labome selects the articles), using Ifng antibody in all methods. It is aimed to help Labome visitors find the most suited Ifng antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Ifng synonym: IFNG2; interferon gamma; IFN-gamma

Invitrogen
mouse monoclonal (DB-1 (DB1))
  • flow cytometry; rat; fig s6e
In order to elucidate the role of B cells in the initiation of central nervous system autoimmunity, Invitrogen Ifng antibody (Biosource, DB-1) was used in flow cytometry on rat samples (fig s6e). Proc Natl Acad Sci U S A (2016) ncbi
mouse monoclonal (DB-1 (DB1))
  • ELISA; rat; loading ...; fig 4b
In order to use rats to study cellular and molecular mechanisms underlying specific sexual dimorphism in T lymphocyte-dependent inflammatory responses, Invitrogen Ifng antibody (eBioscience, DB1) was used in ELISA on rat samples (fig 4b). Brain Behav Immun (2015) ncbi
goat polyclonal
  • western blot; rat; 1:500
  • immunohistochemistry; mouse; 1:100
In order to examine the mechanism of sensorimotor declines in a rat model of repetitive overuse injury, Invitrogen Ifng antibody (Thermo Scientific, PA1-24782) was used in western blot on rat samples at 1:500 and in immunohistochemistry on mouse samples at 1:100. J Cell Commun Signal (2015) ncbi
mouse monoclonal (DB-1 (DB1))
  • immunohistochemistry; rat
In order to test if spreading depression disrupts myelin via interferon gamma and oxidative stress, Invitrogen Ifng antibody (Invitrogen, ARC4033) was used in immunohistochemistry on rat samples . Exp Neurol (2015) ncbi
mouse monoclonal (DB-1 (DB1))
  • ELISA; rat; fig 5
In order to test if HLA-B27 expression on T cells is required for development of colitis, Invitrogen Ifng antibody (Biosource, DB-1) was used in ELISA on rat samples (fig 5). Clin Exp Immunol (2006) ncbi
mouse monoclonal (DB-1 (DB1))
  • ELISA; mouse; fig 4
In order to induce protective immunity against secondary hydatidosis and elucidate the mechanisms of protection, Invitrogen Ifng antibody (Biosource, DB1) was used in ELISA on mouse samples (fig 4). Parasitol Res (2005) ncbi
mouse monoclonal (DB-1 (DB1))
  • ELISA; rat; fig 5
In order to elucidate how prebiotics prevent colitis in specific pathogen-free HLA-B27 rats, Invitrogen Ifng antibody (Biosource, DB-1) was used in ELISA on rat samples (fig 5). Inflamm Bowel Dis (2005) ncbi
mouse monoclonal (DB-1 (DB1))
  • ELISA; rat
In order to examine the regulation of in vitro T-cell responses to enteric bacterial components, Invitrogen Ifng antibody (Biosource, DB-1) was used in ELISA on rat samples . Immunology (2005) ncbi
mouse monoclonal (DB-1 (DB1))
  • ELISA; rat; 1 ug/ml
In order to isolate rat dendritic cells and compare them to human and mouse dendritic cells, Invitrogen Ifng antibody (Biosource, DB-1) was used in ELISA on rat samples at 1 ug/ml. Immunol Lett (2005) ncbi
mouse monoclonal (DB-1 (DB1))
  • ELISA; rat; 10 ug/ml; fig 4
In order to study contribution of T cells to neuropathic pain, Invitrogen Ifng antibody (Biosource, DB-1) was used in ELISA on rat samples at 10 ug/ml (fig 4). Neuroscience (2004) ncbi
mouse monoclonal (DB-1 (DB1))
  • ELISA; rat; fig 3
In order to study the role of intraepithelial NK in autoimmunity, Invitrogen Ifng antibody (BioSource, DB-1) was used in ELISA on rat samples (fig 3). J Immunol (2004) ncbi
mouse monoclonal (DB-1 (DB1))
  • ELISA; mouse
In order to determine the fate of autoreactive CD4+T cells in HNT-TCR x GFAP-HA double transgenic mice, Invitrogen Ifng antibody (Biosource, DB-1) was used in ELISA on mouse samples . J Autoimmun (2004) ncbi
mouse monoclonal (DB-1 (DB1))
  • ELISA; rat; fig 3
In order to use transgenic rats to investigate host-dependent immune responses to commensal caecal bacteria, Invitrogen Ifng antibody (Biosource, DB-1) was used in ELISA on rat samples (fig 3). Clin Exp Immunol (2004) ncbi
mouse monoclonal (DB-1 (DB1))
  • blocking or activating experiments; rat
In order to determine T-bet and GATA-3 expression by semi-quantitative reverse transcription polymerase chain reaction in Th1 and Th2 cells, Invitrogen Ifng antibody (Biosource, DB-1) was used in blocking or activating experiments on rat samples . J Immunol Methods (2003) ncbi
mouse monoclonal (DB-1 (DB1))
  • immunohistochemistry - frozen section; mouse
In order to study herpes simplex virus-induced retinitis, Invitrogen Ifng antibody (BioSource International, clone DB-1) was used in immunohistochemistry - frozen section on mouse samples . J Neuroimmunol (2002) ncbi
mouse monoclonal (DB-1 (DB1))
  • blocking or activating experiments; rat
In order to study a chronic asthma like phenotype in rats, Invitrogen Ifng antibody (Biosource, DB-1) was used in blocking or activating experiments on rat samples . Pediatr Res (2002) ncbi
mouse monoclonal (DB-1 (DB1))
  • ELISA; rat; 10 ug/ml; fig 2
In order to test the effect of bacillus Calmette-Guerin immunization on an allergic inflammation depends on the Th2-type cytokine response, Invitrogen Ifng antibody (Biosource, clone DB-1) was used in ELISA on rat samples at 10 ug/ml (fig 2). Am J Respir Cell Mol Biol (2002) ncbi
mouse monoclonal (DB-1 (DB1))
  • flow cytometry; rat
In order to test the antitumor effect of OK-Lipo on hepatocellular carcinomas, Invitrogen Ifng antibody (Biosource, DB-1) was used in flow cytometry on rat samples . J Gastroenterol Hepatol (2002) ncbi
mouse monoclonal (DB-1 (DB1))
  • ELISA; rat; fig 8
In order to characterize the rat intestinal lymphoid compartment, Invitrogen Ifng antibody (BioSource International, DB-1) was used in ELISA on rat samples (fig 8). J Immunol (2001) ncbi
mouse monoclonal (DB-1 (DB1))
  • ELISA; mouse; fig 1
In order to study gender specific cytokine responses, Invitrogen Ifng antibody (BioSource International, DB-1) was used in ELISA on mouse samples (fig 1). J Immunol (2001) ncbi
mouse monoclonal (DB-1 (DB1))
  • flow cytometry; rat
In order to use flow cytometry to characterize rat T cells, Invitrogen Ifng antibody (Biosource, DB-1) was used in flow cytometry on rat samples . J Immunol Methods (2000) ncbi
mouse monoclonal (DB-1 (DB1))
  • immunohistochemistry - paraffin section; rat; fig 6
In order to characterize a hamster-rat model of discordant xenotransplantation, Invitrogen Ifng antibody (Biosource, DB-1) was used in immunohistochemistry - paraffin section on rat samples (fig 6). J Immunol (2000) ncbi
mouse monoclonal (DB-1 (DB1))
  • ELISA; rat; 2 ug/ml; fig 1
In order to study migration of Th1 and Th2 cells, Invitrogen Ifng antibody (BioSource International, DB-1) was used in ELISA on rat samples at 2 ug/ml (fig 1). J Immunol (1999) ncbi
mouse monoclonal (DB-1 (DB1))
  • ELISA; rat
In order to study Th2 responses in a rat model of schistosomiasis, Invitrogen Ifng antibody (Biosource, DB-1) was used in ELISA on rat samples . Infect Immun (1999) ncbi
mouse monoclonal (DB-1 (DB1))
  • immunohistochemistry - paraffin section; rat
In order to determine the cellular mechanism of xenograft rejection in rats, Invitrogen Ifng antibody (Biosource, DB-1) was used in immunohistochemistry - paraffin section on rat samples . J Immunol (1999) ncbi
mouse monoclonal (DB-1 (DB1))
  • immunohistochemistry; rat
In order to test if xenografts would not be rejected upon retransplantation into second recipients treated with cyclosporine, Invitrogen Ifng antibody (biosource, DB-1) was used in immunohistochemistry on rat samples . Transplantation (1998) ncbi
mouse monoclonal (DB-1 (DB1))
  • ELISA; mouse; fig 1
In order to study cytokine production by splenic cells stimulated with phagocytosable-size chitin particles, Invitrogen Ifng antibody (Biosource, DB-1) was used in ELISA on mouse samples (fig 1). J Immunol (1997) ncbi
mouse monoclonal (DB-1 (DB1))
  • ELISA; mouse
In order to study the effects of phagocytosable chitin particles on alveolar macrophages, Invitrogen Ifng antibody (Biosource, DB-1) was used in ELISA on mouse samples . Infect Immun (1997) ncbi
mouse monoclonal (DB-1 (DB1))
  • blocking or activating experiments; mouse
In order to assess the role of antigen-specific, IFN-gamma-producing TH1 cells in murine Pseudomonas aeruginosa infection, Invitrogen Ifng antibody (Biosource, DB-1) was used in blocking or activating experiments on mouse samples . Infect Immun (1995) ncbi
BioLegend
mouse monoclonal (DB-1)
  • flow cytometry; rat; loading ...; fig s4b
BioLegend Ifng antibody (Biolegend, DB-1) was used in flow cytometry on rat samples (fig s4b). Nature (2019) ncbi
mouse monoclonal (DB-1)
  • flow cytometry; rat; loading ...; fig 4c
BioLegend Ifng antibody (BioLegend, DB-1) was used in flow cytometry on rat samples (fig 4c). Eur J Immunol (2017) ncbi
mouse monoclonal (DB-1)
  • western blot; rat
BioLegend Ifng antibody (Biolegend, 507802) was used in western blot on rat samples . Int J Clin Exp Pathol (2015) ncbi
mouse monoclonal (DB-1)
BioLegend Ifng antibody (BioLegend, DB-1) was used . J Immunol (2014) ncbi
Abcam
rabbit monoclonal (EPR1108)
  • immunocytochemistry; mouse; 1:200; loading ...; fig 3e
In order to study the role of CUGBP1 in the profibrotic TGF-beta-dependent activation of hepatic stellate cells, Abcam Ifng antibody (Abcam, ab133566) was used in immunocytochemistry on mouse samples at 1:200 (fig 3e). Nat Commun (2016) ncbi
Santa Cruz Biotechnology
mouse monoclonal (DB-14)
  • western blot; mouse; 1:1000; fig 3
Santa Cruz Biotechnology Ifng antibody (Santa Cruz Biotechnology, sc-59992) was used in western blot on mouse samples at 1:1000 (fig 3). Sci Rep (2015) ncbi
mouse monoclonal (DB-14)
  • western blot; mouse; fig 5
Santa Cruz Biotechnology Ifng antibody (Santa Cruz, sc-59992) was used in western blot on mouse samples (fig 5). Laryngoscope (2014) ncbi
Articles Reviewed
  1. Lodygin D, Hermann M, Schweingruber N, Flügel Koch C, Watanabe T, Schlosser C, et al. β-Synuclein-reactive T cells induce autoimmune CNS grey matter degeneration. Nature. 2019;566:503-508 pubmed publisher
  2. Tuncel J, Haag S, Holmdahl R. MHC class II alleles associated with Th1 rather than Th17 type immunity drive the onset of early arthritis in a rat model of rheumatoid arthritis. Eur J Immunol. 2017;47:563-574 pubmed publisher
  3. Wu X, Wu X, Ma Y, Shao F, Tan Y, Tan T, et al. CUG-binding protein 1 regulates HSC activation and liver fibrogenesis. Nat Commun. 2016;7:13498 pubmed publisher
  4. Flach A, Litke T, Strauss J, Haberl M, Gómez C, Reindl M, et al. Autoantibody-boosted T-cell reactivation in the target organ triggers manifestation of autoimmune CNS disease. Proc Natl Acad Sci U S A. 2016;113:3323-8 pubmed publisher
  5. Fiume G, Scialdone A, Albano F, Rossi A, Tuccillo F, Rea D, et al. Impairment of T cell development and acute inflammatory response in HIV-1 Tat transgenic mice. Sci Rep. 2015;5:13864 pubmed publisher
  6. Garcia P, Apolinário L, Böckelmann P, da Silva Nunes I, Duran N, Fávaro W. Alterations in ubiquitin ligase Siah-2 and its corepressor N-CoR after P-MAPA immunotherapy and anti-androgen therapy: new therapeutic opportunities for non-muscle invasive bladder cancer. Int J Clin Exp Pathol. 2015;8:4427-43 pubmed
  7. Nacka Aleksić M, Djikić J, Pilipović I, Stojić Vukanić Z, Kosec D, Bufan B, et al. Male rats develop more severe experimental autoimmune encephalomyelitis than female rats: sexual dimorphism and diergism at the spinal cord level. Brain Behav Immun. 2015;49:101-18 pubmed publisher
  8. Fisher P, Zhao Y, Rico M, Massicotte V, WADE C, Litvin J, et al. Increased CCN2, substance P and tissue fibrosis are associated with sensorimotor declines in a rat model of repetitive overuse injury. J Cell Commun Signal. 2015;9:37-54 pubmed publisher
  9. Pusic A, Mitchell H, Kunkler P, Klauer N, Kraig R. Spreading depression transiently disrupts myelin via interferon-gamma signaling. Exp Neurol. 2015;264:43-54 pubmed publisher
  10. Kim B, Park H, Shin J, Kim S, Kim S. Human placental extract reduces allergic inflammation in a murine allergic rhinitis model. Laryngoscope. 2014;124:E399-404 pubmed publisher
  11. Chao G, Wallis R, Marandi L, Ning T, Sarmiento J, Paterson A, et al. Iddm30 controls pancreatic expression of Ccl11 (Eotaxin) and the Th1/Th2 balance within the insulitic lesions. J Immunol. 2014;192:3645-53 pubmed publisher
  12. Hoentjen F, Tonkonogy S, Liu B, Sartor R, Taurog J, Dieleman L. Adoptive transfer of nontransgenic mesenteric lymph node cells induces colitis in athymic HLA-B27 transgenic nude rats. Clin Exp Immunol. 2006;143:474-83 pubmed
  13. Al Qaoud K, Abdel Hafez S. Humoral and cytokine response during protection of mice against secondary hydatidosis caused by Echinococcus granulosus. Parasitol Res. 2005;98:54-60 pubmed
  14. Hoentjen F, Welling G, Harmsen H, Zhang X, Snart J, Tannock G, et al. Reduction of colitis by prebiotics in HLA-B27 transgenic rats is associated with microflora changes and immunomodulation. Inflamm Bowel Dis. 2005;11:977-85 pubmed
  15. Qian B, Tonkonogy S, Hoentjen F, Dieleman L, Sartor R. Dysregulated luminal bacterial antigen-specific T-cell responses and antigen-presenting cell function in HLA-B27 transgenic rats with chronic colitis. Immunology. 2005;116:112-21 pubmed
  16. Janelidze S, Enell K, Visse E, Darabi A, Salford L, Siesjo P. Activation of purified allogeneic CD4(+) T cells by rat bone marrow-derived dendritic cells induces concurrent secretion of IFN-gamma, IL-4, and IL-10. Immunol Lett. 2005;101:193-201 pubmed
  17. Moalem G, Xu K, Yu L. T lymphocytes play a role in neuropathic pain following peripheral nerve injury in rats. Neuroscience. 2004;129:767-77 pubmed
  18. Todd D, Forsberg E, Greiner D, Mordes J, Rossini A, Bortell R. Deficiencies in gut NK cell number and function precede diabetes onset in BB rats. J Immunol. 2004;172:5356-62 pubmed
  19. Cabarrocas J, Piaggio E, Zappulla J, Desbois S, Mars L, Lassmann H, et al. A transgenic mouse model for T-cell ignorance of a glial autoantigen. J Autoimmun. 2004;22:179-89 pubmed
  20. Dieleman L, Hoentjen F, Qian B, Sprengers D, Tjwa E, Torres M, et al. Reduced ratio of protective versus proinflammatory cytokine responses to commensal bacteria in HLA-B27 transgenic rats. Clin Exp Immunol. 2004;136:30-9 pubmed
  21. Chakir H, Wang H, Lefebvre D, Webb J, Scott F. T-bet/GATA-3 ratio as a measure of the Th1/Th2 cytokine profile in mixed cell populations: predominant role of GATA-3. J Immunol Methods. 2003;278:157-69 pubmed
  22. Archin N, Atherton S. Infiltration of T-lymphocytes in the brain after anterior chamber inoculation of a neurovirulent and neuroinvasive strain of HSV-1. J Neuroimmunol. 2002;130:117-27 pubmed
  23. Sorkness R, Castleman W, Mikus L, Mosser A, Lemanske R. Chronic postbronchiolitis airway instability induced with anti-IFN-gamma antibody in F344 rats. Pediatr Res. 2002;52:382-6 pubmed
  24. Hylkema M, Timens W, Luinge M, Van Der Werf N, Hoekstra M. The effect of bacillus Calmette-Guérin immunization depends on the genetic predisposition to Th2-type responsiveness. Am J Respir Cell Mol Biol. 2002;27:244-9 pubmed
  25. Uehara K, Ichida T, Sugahara S, Ishikawa T, Yamagiwa S, Yoshida Y, et al. Systemic administration of liposome-encapsulated OK-432 prolongs the survival of rats with hepatocellular carcinoma through the induction of IFN-gamma-producing hepatic lymphocytes. J Gastroenterol Hepatol. 2002;17:81-90 pubmed
  26. Todd D, Greiner D, Rossini A, Mordes J, Bortell R. An atypical population of NK cells that spontaneously secrete IFN-gamma and IL-4 is present in the intraepithelial lymphoid compartment of the rat. J Immunol. 2001;167:3600-9 pubmed
  27. Liva S, Voskuhl R. Testosterone acts directly on CD4+ T lymphocytes to increase IL-10 production. J Immunol. 2001;167:2060-7 pubmed
  28. Caraher E, Parenteau M, Gruber H, Scott F. Flow cytometric analysis of intracellular IFN-gamma, IL-4 and IL-10 in CD3(+)4(+) T-cells from rat spleen. J Immunol Methods. 2000;244:29-40 pubmed
  29. Lin Y, Soares M, Sato K, Csizmadia E, Robson S, Smith N, et al. Long-term survival of hamster hearts in presensitized rats. J Immunol. 2000;164:4883-92 pubmed
  30. Kawai T, Seki M, Hiromatsu K, Eastcott J, Watts G, Sugai M, et al. Selective diapedesis of Th1 cells induced by endothelial cell RANTES. J Immunol. 1999;163:3269-78 pubmed
  31. Cetre C, Pierrot C, Cocude C, Lafitte S, Capron A, Capron M, et al. Profiles of Th1 and Th2 cytokines after primary and secondary infection by Schistosoma mansoni in the semipermissive rat host. Infect Immun. 1999;67:2713-9 pubmed
  32. Lin Y, Soares M, Sato K, Takigami K, Csizmadia E, Anrather J, et al. Rejection of cardiac xenografts by CD4+ or CD8+ T cells. J Immunol. 1999;162:1206-14 pubmed
  33. Miyatake T, Koyamada N, Hancock W, Soares M, Bach F. Survival of accommodated cardiac xenografts upon retransplantation into cyclosporine-treated recipients. Transplantation. 1998;65:1563-9 pubmed
  34. Shibata Y, Metzger W, Myrvik Q. Chitin particle-induced cell-mediated immunity is inhibited by soluble mannan: mannose receptor-mediated phagocytosis initiates IL-12 production. J Immunol. 1997;159:2462-7 pubmed
  35. Shibata Y, Foster L, Metzger W, Myrvik Q. Alveolar macrophage priming by intravenous administration of chitin particles, polymers of N-acetyl-D-glucosamine, in mice. Infect Immun. 1997;65:1734-41 pubmed
  36. Früh R, Blum B, Mossmann H, Domdey H, von Specht B. TH1 cells trigger tumor necrosis factor alpha-mediated hypersensitivity to Pseudomonas aeruginosa after adoptive transfer into SCID mice. Infect Immun. 1995;63:1107-12 pubmed