This is a Validated Antibody Database (VAD) review about rat Hspa4, based on 38 published articles (read how Labome selects the articles), using Hspa4 antibody in all methods. It is aimed to help Labome visitors find the most suited Hspa4 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Hspa4 synonym: Hsp110; Hsp70; irp94

Invitrogen
mouse monoclonal (3A3)
  • western blot; human; 1:200; loading ...; fig 5
In order to elucidate the antiproliferative and pro-apoptotic effects of thiazine[6,5-b]indole, Invitrogen Hspa4 antibody (Affinity BioReagents, MA3-006) was used in western blot on human samples at 1:200 (fig 5). Biomed Pharmacother (2017) ncbi
mouse monoclonal (3A3)
In order to measure biochemical and organismal responses to acute heat stress on two neritic copepods, Invitrogen Hspa4 antibody (Thermo Scientific, MA3-006) was used . Comp Biochem Physiol A Mol Integr Physiol (2017) ncbi
mouse monoclonal (2A4)
  • western blot; human; 1:5000; loading ...; fig 1a
In order to demonstrate the bipartite role of Hsp90 in chaperoning CRAF kinase, Invitrogen Hspa4 antibody (Thermo Pierce, MA3-008) was used in western blot on human samples at 1:5000 (fig 1a). J Biol Chem (2016) ncbi
mouse monoclonal (3A3)
  • western blot; Artemia franciscana; 1:5000; fig 2a
  • western blot; human; 1:5000
In order to test if exposure to non-lethal heat shock increases the tolerance of Artemia franciscana to subsequent metal exposure, Invitrogen Hspa4 antibody (Affinity BioReagents, 3A3) was used in western blot on Artemia franciscana samples at 1:5000 (fig 2a) and in western blot on human samples at 1:5000. Environ Res (2016) ncbi
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig 6a
In order to test if Hsp70 and the 20S proteasome cooperate to selectively degrade oxidatively damaged proteins, Invitrogen Hspa4 antibody (Thermo Fisher Scientific, PA5-28003) was used in western blot on mouse samples (fig 6a). Free Radic Biol Med (2016) ncbi
mouse monoclonal (3A3)
  • western blot; mouse; 1:1000; loading ...; fig 3c
In order to investigate the function of extended synaptotagmins in knock out mice, Invitrogen Hspa4 antibody (Thermo Scientific, MA3-0066) was used in western blot on mouse samples at 1:1000 (fig 3c). PLoS ONE (2016) ncbi
mouse monoclonal (MB-H1)
  • immunohistochemistry - paraffin section; human; 1:40; tbl 3
In order to investigate evidence for two distinct pathogenetic pathways by studing the squamous cell carcinoma of the oral cavity and cytokeratin and protein expression patterns, Invitrogen Hspa4 antibody (Invitrogen, 33-3800) was used in immunohistochemistry - paraffin section on human samples at 1:40 (tbl 3). Oncol Lett (2016) ncbi
mouse monoclonal (3A3)
In order to assess the effect of salinity on catfish's susceptibility to bacterial infection, Invitrogen Hspa4 antibody (ThermoScientific, 3A3) was used . Fish Shellfish Immunol (2016) ncbi
mouse monoclonal (3A3)
  • western blot; Pacific oyster; 1:2500; fig 1
In order to study salinity and thermal stress in wild and farmed Pacific oysters Crassostrea gigas, Invitrogen Hspa4 antibody (Affinity BioReagents, MA3-006) was used in western blot on Pacific oyster samples at 1:2500 (fig 1). Comp Biochem Physiol A Mol Integr Physiol (2016) ncbi
mouse monoclonal (5A5)
  • western blot; scallops; fig 7
In order to determine the affect of cellular bioenergetics in marine molluscs and functional reorganization of mitochondria by intermittent hypoxia, Invitrogen Hspa4 antibody (Affinity Bioreagents, MA3-007) was used in western blot on scallops samples (fig 7). J Exp Biol (2016) ncbi
mouse monoclonal (5A5)
  • western blot; human; fig 1
In order to calculate how ionizing radiation can promote cell survival in exosomes derived from squamous head and neck cancer, Invitrogen Hspa4 antibody (Affinity Bioreagents, MA3-007) was used in western blot on human samples (fig 1). PLoS ONE (2016) ncbi
mouse monoclonal (3A3)
  • western blot; mouse; 1:5000; fig 1
In order to determine the affects of dendritic cell migration in vitro by loss of gadkin, Invitrogen Hspa4 antibody (Affinity Bioreagents, MA3006) was used in western blot on mouse samples at 1:5000 (fig 1). PLoS ONE (2015) ncbi
mouse monoclonal (3A3)
  • western blot; common limpet; 1:1000
In order to examine the effect of sun exposure on of thermal refugia, Invitrogen Hspa4 antibody (Thermo Scientific, MA3-006) was used in western blot on common limpet samples at 1:1000. Glob Chang Biol (2016) ncbi
mouse monoclonal (3A3)
  • western blot; Asian green mussel; 1:5000; fig 2
In order to test if non-lethal heat shock of Perna viridis promotes the production of Hsp70 and enhances its resistance to stresses, Invitrogen Hspa4 antibody (Thermo Scientific, MA3-006) was used in western blot on Asian green mussel samples at 1:5000 (fig 2). PLoS ONE (2015) ncbi
domestic rabbit polyclonal
In order to identify proteins in syncytiotrophoblast extracellular vesicles and determine their role in preeclampsia, Invitrogen Hspa4 antibody (Thermo Fisher, PA5-28003) was used . Cell Physiol Biochem (2015) ncbi
mouse monoclonal (3A3)
  • immunohistochemistry; holothurians; 1:200
  • western blot; holothurians; 1:1000
In order to study HSP70 in Apostichopus japonicus, Invitrogen Hspa4 antibody (Thermo, MA3-006) was used in immunohistochemistry on holothurians samples at 1:200 and in western blot on holothurians samples at 1:1000. Fish Shellfish Immunol (2015) ncbi
mouse monoclonal (5A5)
  • western blot; mouse
In order to elucidate the factors that mediate radiation-induced endothelial dysfunction, Invitrogen Hspa4 antibody (scbt, MA3-007) was used in western blot on mouse samples . J Proteome Res (2015) ncbi
mouse monoclonal (3A3)
  • western blot; mouse; fig 2
In order to study the temporal regulation of MyoD in skeletal muscle differentiation, Invitrogen Hspa4 antibody (ABR Affinity Bio Reagents, MA3-006) was used in western blot on mouse samples (fig 2). Dev Dyn (2015) ncbi
mouse monoclonal (5A5)
  • ELISA; fruit fly ; 1:1000; fig 3
In order to test the thermal co-adaptation hypothesis using Drosophila subobscura, Invitrogen Hspa4 antibody (Thermo Scientific, 5A5) was used in ELISA on fruit fly samples at 1:1000 (fig 3). J Evol Biol (2012) ncbi
mouse monoclonal (5A5)
  • ELISA; Lycaena tityrus; 1:700; loading ...; fig 2c
In order to evaluate if the use of constant temperatures is justified when studying Lycaena tityrus, Invitrogen Hspa4 antibody (Affinity BioReagents, 5A5) was used in ELISA on Lycaena tityrus samples at 1:700 (fig 2c). Oecologia (2011) ncbi
mouse monoclonal (3A3)
  • western blot; Marenzelleria viridis; 1:3000; fig 7a
  • western blot; human; 1:3000
In order to study the effect of acclimation salinity on the stress protein response in two sibling species of Marenzelleria, Invitrogen Hspa4 antibody (Affinity BioReagents, MA3-006) was used in western blot on Marenzelleria viridis samples at 1:3000 (fig 7a) and in western blot on human samples at 1:3000. Comp Biochem Physiol B Biochem Mol Biol (2006) ncbi
mouse monoclonal (3A3)
  • western blot; rat; loading ...; fig 2a
In order to find factors that interact with N-myristoylated Arf6, Invitrogen Hspa4 antibody (Affinity Bioreagents, 3A3) was used in western blot on rat samples (fig 2a). Methods Enzymol (2005) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig 4a
Cell Signaling Technology Hspa4 antibody (Cell Signaling Technology, 3303) was used in western blot on mouse samples (fig 4a). Cell Death Dis (2021) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 1c
Cell Signaling Technology Hspa4 antibody (Cell Signaling, 3303) was used in western blot on human samples (fig 1c). Cells (2019) ncbi
domestic rabbit polyclonal
  • western blot; human; fig 1e
Cell Signaling Technology Hspa4 antibody (Cell Signaling, 3303) was used in western blot on human samples (fig 1e). Mol Vis (2017) ncbi
BD Biosciences
mouse monoclonal (7/Hsp70)
  • western blot; mouse; loading ...; fig 2b
  • western blot; human; loading ...; fig s3b
BD Biosciences Hspa4 antibody (BD Pharmingen, 610607) was used in western blot on mouse samples (fig 2b) and in western blot on human samples (fig s3b). Cancer Sci (2022) ncbi
mouse monoclonal (7/Hsp70)
  • western blot; human; loading ...; fig 2a
BD Biosciences Hspa4 antibody (BD Biosciences, 610607) was used in western blot on human samples (fig 2a). Sci Adv (2019) ncbi
mouse monoclonal (21/Hsp110)
  • western blot; dogs; 1:500; loading ...; fig 3
BD Biosciences Hspa4 antibody (BD Biosciences, 610511) was used in western blot on dogs samples at 1:500 (fig 3). Int J Hyperthermia (2017) ncbi
mouse monoclonal (7/Hsp70)
  • western blot; human; loading ...; fig 5d
In order to identify homologous to the E6-AP carboxyl terminus as a ubiquitin ligase for modulator of apoptosis protein 1, BD Biosciences Hspa4 antibody (BD Biosciences, 610607) was used in western blot on human samples (fig 5d). Oncogene (2017) ncbi
mouse monoclonal (7/Hsp70)
  • western blot; human; fig 2a
In order to develop a first-in-class humanized antibody against PRL-3 and assess its use as a treatment for gastric cancer, BD Biosciences Hspa4 antibody (BD Pharmingen, 610607) was used in western blot on human samples (fig 2a). JCI Insight (2016) ncbi
mouse monoclonal (7/Hsp70)
  • western blot; mouse
BD Biosciences Hspa4 antibody (BD Transduction, 610608) was used in western blot on mouse samples . Oncoimmunology (2016) ncbi
mouse monoclonal (7/Hsp70)
  • western blot; human; fig 3
In order to study pVHL-mediated degradation of B-Myb and hypoxia-inducible factor alpha by parallele regulation of von Hippel-Lindau disease, BD Biosciences Hspa4 antibody (BD Transduction Laboratories, 610607) was used in western blot on human samples (fig 3). Mol Cell Biol (2016) ncbi
mouse monoclonal (7/Hsp70)
  • western blot; human; fig 1b
BD Biosciences Hspa4 antibody (BD Transduction Laboratories, 610607) was used in western blot on human samples (fig 1b). Autophagy (2015) ncbi
mouse monoclonal (7/Hsp70)
  • western blot; human; fig 1
BD Biosciences Hspa4 antibody (Transduction Laboratories, 610608) was used in western blot on human samples (fig 1). J Extracell Vesicles (2015) ncbi
mouse monoclonal (7/Hsp70)
  • western blot; human
BD Biosciences Hspa4 antibody (BD Biosciences, 610607) was used in western blot on human samples . Oncotarget (2015) ncbi
mouse monoclonal (7/Hsp70)
  • western blot; chicken; 1:1000
BD Biosciences Hspa4 antibody (BD Bioscience, 610607) was used in western blot on chicken samples at 1:1000. Biosci Biotechnol Biochem (2015) ncbi
mouse monoclonal (7/Hsp70)
  • western blot; rat
BD Biosciences Hspa4 antibody (Transduction Laboratories, 610607) was used in western blot on rat samples . World J Gastroenterol (2015) ncbi
mouse monoclonal (7/Hsp70)
  • western blot; human; fig 6
BD Biosciences Hspa4 antibody (BD, 610607) was used in western blot on human samples (fig 6). J Virol (2011) ncbi
Articles Reviewed
  1. Wang Q, Chen Y, Xie Y, Yang D, Sun Y, Yuan Y, et al. Histone H1.2 promotes hepatocarcinogenesis by regulating signal transducer and activator of transcription 3 signaling. Cancer Sci. 2022;113:1679-1692 pubmed publisher
  2. Zhao F, Zheng T, Gong W, Wu J, Xie H, Li W, et al. Extracellular vesicles package dsDNA to aggravate Crohn's disease by activating the STING pathway. Cell Death Dis. 2021;12:815 pubmed publisher
  3. Barbero G, Castro M, Villanueva M, Quezada M, Fernández N, Demorrow S, et al. An Autocrine Wnt5a Loop Promotes NF-κB Pathway Activation and Cytokine/Chemokine Secretion in Melanoma. Cells. 2019;8: pubmed publisher
  4. May J, Kouri F, Hurley L, Liu J, Tommasini Ghelfi S, Ji Y, et al. IDH3α regulates one-carbon metabolism in glioblastoma. Sci Adv. 2019;5:eaat0456 pubmed publisher
  5. Liu L, Jiang Y, Chahine A, Curtiss E, Steinle J. Epac1 agonist decreased inflammatory proteins in retinal endothelial cells, and loss of Epac1 increased inflammatory proteins in the retinal vasculature of mice. Mol Vis. 2017;23:1-7 pubmed
  6. Solárová Z, Kello M, Varinska L, Budovská M, Solar P. Inhibition of heat shock protein (Hsp) 90 potentiates the antiproliferative and pro-apoptotic effects of 2-(4'fluoro-phenylamino)-4H-1,3-thiazine[6,5-b]indole in A2780cis cells. Biomed Pharmacother. 2017;85:463-471 pubmed publisher
  7. Graner A, Hellwinkel J, Lencioni A, Madsen H, Harland T, Marchando P, et al. HSP90 inhibitors in the context of heat shock and the unfolded protein response: effects on a primary canine pulmonary adenocarcinoma cell line. Int J Hyperthermia. 2017;33:303-317 pubmed publisher
  8. Rahlff J, Peters J, Moyano M, Pless O, Claussen C, Peck M. Short-term molecular and physiological responses to heat stress in neritic copepods Acartia tonsa and Eurytemora affinis. Comp Biochem Physiol A Mol Integr Physiol. 2017;203:348-358 pubmed publisher
  9. Matsuura K, Huang N, Cocce K, Zhang L, Kornbluth S. Downregulation of the proapoptotic protein MOAP-1 by the UBR5 ubiquitin ligase and its role in ovarian cancer resistance to cisplatin. Oncogene. 2017;36:1698-1706 pubmed publisher
  10. Mitra S, Ghosh B, Gayen N, Roy J, Mandal A. Bipartite Role of Heat Shock Protein 90 (Hsp90) Keeps CRAF Kinase Poised for Activation. J Biol Chem. 2016;291:24579-24593 pubmed
  11. Thura M, Al Aidaroos A, Yong W, Kono K, Gupta A, Lin Y, et al. PRL3-zumab, a first-in-class humanized antibody for cancer therapy. JCI Insight. 2016;1:e87607 pubmed publisher
  12. Pestana J, Novais S, Norouzitallab P, Vandegehuchte M, Bossier P, De Schamphelaere K. Non-lethal heat shock increases tolerance to metal exposure in brine shrimp. Environ Res. 2016;151:663-670 pubmed publisher
  13. Reeg S, Jung T, Castro J, Davies K, Henze A, Grune T. The molecular chaperone Hsp70 promotes the proteolytic removal of oxidatively damaged proteins by the proteasome. Free Radic Biol Med. 2016;99:153-166 pubmed publisher
  14. Finkel P, Frey B, Mayer F, Bösl K, Werthmöller N, Mackensen A, et al. The dual role of NK cells in antitumor reactions triggered by ionizing radiation in combination with hyperthermia. Oncoimmunology. 2016;5:e1101206 pubmed publisher
  15. Sclip A, Bacaj T, Giam L, Sudhof T. Extended Synaptotagmin (ESyt) Triple Knock-Out Mice Are Viable and Fertile without Obvious Endoplasmic Reticulum Dysfunction. PLoS ONE. 2016;11:e0158295 pubmed publisher
  16. Frohwitter G, Buerger H, van Diest P, Korsching E, Kleinheinz J, Fillies T. Cytokeratin and protein expression patterns in squamous cell carcinoma of the oral cavity provide evidence for two distinct pathogenetic pathways. Oncol Lett. 2016;12:107-113 pubmed
  17. Schmitz M, Douxfils J, Mandiki S, Morana C, Baekelandt S, Kestemont P. Chronic hyperosmotic stress interferes with immune homeostasis in striped catfish (Pangasianodon hypophthalmus, S.) and leads to excessive inflammatory response during bacterial infection. Fish Shellfish Immunol. 2016;55:550-8 pubmed publisher
  18. Yang C, Sierp M, Abbott C, Li Y, Qin J. Responses to thermal and salinity stress in wild and farmed Pacific oysters Crassostrea gigas. Comp Biochem Physiol A Mol Integr Physiol. 2016;201:22-29 pubmed publisher
  19. Ivanina A, Nesmelova I, Leamy L, Sokolov E, Sokolova I. Intermittent hypoxia leads to functional reorganization of mitochondria and affects cellular bioenergetics in marine molluscs. J Exp Biol. 2016;219:1659-74 pubmed publisher
  20. Okumura F, Uematsu K, Byrne S, Hirano M, Joo Okumura A, Nishikimi A, et al. Parallel Regulation of von Hippel-Lindau Disease by pVHL-Mediated Degradation of B-Myb and Hypoxia-Inducible Factor ?. Mol Cell Biol. 2016;36:1803-17 pubmed publisher
  21. Mutschelknaus L, Peters C, Winkler K, Yentrapalli R, Heider T, Atkinson M, et al. Exosomes Derived from Squamous Head and Neck Cancer Promote Cell Survival after Ionizing Radiation. PLoS ONE. 2016;11:e0152213 pubmed publisher
  22. Schachtner H, Weimershaus M, Stache V, Plewa N, Legler D, Höpken U, et al. Loss of Gadkin Affects Dendritic Cell Migration In Vitro. PLoS ONE. 2015;10:e0143883 pubmed publisher
  23. Lima F, Gomes F, Seabra R, Wethey D, Seabra M, Cruz T, et al. Loss of thermal refugia near equatorial range limits. Glob Chang Biol. 2016;22:254-63 pubmed publisher
  24. Aleng N, Sung Y, MacRae T, Abd Wahid M. Non-Lethal Heat Shock of the Asian Green Mussel, Perna viridis, Promotes Hsp70 Synthesis, Induces Thermotolerance and Protects Against Vibrio Infection. PLoS ONE. 2015;10:e0135603 pubmed publisher
  25. Hieke N, Löffler A, Kaizuka T, Berleth N, Böhler P, Drießen S, et al. Expression of a ULK1/2 binding-deficient ATG13 variant can partially restore autophagic activity in ATG13-deficient cells. Autophagy. 2015;11:1471-83 pubmed publisher
  26. Lobb R, Becker M, Wen S, Wong C, Wiegmans A, Leimgruber A, et al. Optimized exosome isolation protocol for cell culture supernatant and human plasma. J Extracell Vesicles. 2015;4:27031 pubmed publisher
  27. Pulvino M, Chen L, Oleksyn D, Li J, Compitello G, Rossi R, et al. Inhibition of COP9-signalosome (CSN) deneddylating activity and tumor growth of diffuse large B-cell lymphomas by doxycycline. Oncotarget. 2015;6:14796-813 pubmed
  28. Ueda S, Kokaji Y, Simizu S, Honda K, Yoshino K, Kamisoyama H, et al. Chicken heat shock protein HSPB1 increases and interacts with αB-crystallin in aged skeletal muscle. Biosci Biotechnol Biochem. 2015;79:1867-75 pubmed publisher
  29. Li H, Han L, Yang Z, Huang W, Zhang X, Gu Y, et al. Differential Proteomic Analysis of Syncytiotrophoblast Extracellular Vesicles from Early-Onset Severe Preeclampsia, using 8-Plex iTRAQ Labeling Coupled with 2D Nano LC-MS/MS. Cell Physiol Biochem. 2015;36:1116-30 pubmed publisher
  30. Xu D, Sun L, Liu S, Zhang L, Yang H. Histological, ultrastructural and heat shock protein 70 (HSP70) responses to heat stress in the sea cucumber Apostichopus japonicus. Fish Shellfish Immunol. 2015;45:321-6 pubmed publisher
  31. Pantazi E, Zaouali M, Bejaoui M, Folch Puy E, Ben Abdennebi H, Varela A, et al. Sirtuin 1 in rat orthotopic liver transplantation: an IGL-1 preservation solution approach. World J Gastroenterol. 2015;21:1765-74 pubmed publisher
  32. Azimzadeh O, Sievert W, Sarioglu H, Merl Pham J, Yentrapalli R, Bakshi M, et al. Integrative proteomics and targeted transcriptomics analyses in cardiac endothelial cells unravel mechanisms of long-term radiation-induced vascular dysfunction. J Proteome Res. 2015;14:1203-19 pubmed publisher
  33. Cho O, Mallappa C, Hernández Hernández J, Rivera Pérez J, Imbalzano A. Contrasting roles for MyoD in organizing myogenic promoter structures during embryonic skeletal muscle development. Dev Dyn. 2015;244:43-55 pubmed publisher
  34. Calabria G, Dolgova O, Rego C, Castañeda L, Rezende E, Balanya J, et al. Hsp70 protein levels and thermotolerance in Drosophila subobscura: a reassessment of the thermal co-adaptation hypothesis. J Evol Biol. 2012;25:691-700 pubmed publisher
  35. Ariumi Y, Kuroki M, Kushima Y, Osugi K, Hijikata M, Maki M, et al. Hepatitis C virus hijacks P-body and stress granule components around lipid droplets. J Virol. 2011;85:6882-92 pubmed publisher
  36. Fischer K, Kölzow N, Höltje H, Karl I. Assay conditions in laboratory experiments: is the use of constant rather than fluctuating temperatures justified when investigating temperature-induced plasticity?. Oecologia. 2011;166:23-33 pubmed publisher
  37. Blank M, Bastrop R, Jürss K. Stress protein response in two sibling species of Marenzelleria (Polychaeta: Spionidae): is there an influence of acclimation salinity?. Comp Biochem Physiol B Biochem Mol Biol. 2006;144:451-62 pubmed
  38. Krauss M, Haucke V. Functional assay of effectors of ADP ribosylation factor 6 during clathrin/AP-2 coat recruitment to membranes. Methods Enzymol. 2005;404:388-98 pubmed