This is a Validated Antibody Database (VAD) review about rat Hist1h2aa, based on 34 published articles (read how Labome selects the articles), using Hist1h2aa antibody in all methods. It is aimed to help Labome visitors find the most suited Hist1h2aa antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Hist1h2aa synonym: Th2a; histone H2A type 4; histone H2A, testis; histone cluster 1, H2aa; testis-specific histone 2a

Cell Signaling Technology
rabbit monoclonal (D27C4)
  • immunocytochemistry; human; 1:100; loading ...; fig s2a
Cell Signaling Technology Hist1h2aa antibody (Cell Signaling, 8240) was used in immunocytochemistry on human samples at 1:100 (fig s2a). Nucleic Acids Res (2019) ncbi
rabbit monoclonal (D27C4)
  • ChIP-Seq; mouse; loading ...; fig 1f
  • western blot; mouse; loading ...; fig 1d
Cell Signaling Technology Hist1h2aa antibody (Cell Signaling, 8240) was used in ChIP-Seq on mouse samples (fig 1f) and in western blot on mouse samples (fig 1d). Mol Cell (2019) ncbi
rabbit monoclonal (D27C4)
  • chromatin immunoprecipitation; mouse; loading ...; fig 2f
  • western blot; mouse; loading ...; fig 2a, 4d, s6f
Cell Signaling Technology Hist1h2aa antibody (Cell Signaling, 8240) was used in chromatin immunoprecipitation on mouse samples (fig 2f) and in western blot on mouse samples (fig 2a, 4d, s6f). Science (2019) ncbi
rabbit monoclonal (D27C4)
  • ChIP-Seq; mouse; loading ...; fig 4b
  • western blot; mouse; 1:1000; loading ...; fig 1b
Cell Signaling Technology Hist1h2aa antibody (Cell Signaling, 8240) was used in ChIP-Seq on mouse samples (fig 4b) and in western blot on mouse samples at 1:1000 (fig 1b). Nat Commun (2019) ncbi
rabbit monoclonal (D27C4)
  • ChIP-Seq; mouse; loading ...; fig 3d
Cell Signaling Technology Hist1h2aa antibody (Cell signaling, 8240S) was used in ChIP-Seq on mouse samples (fig 3d). Nat Commun (2019) ncbi
rabbit monoclonal (D27C4)
  • western blot; human; loading ...; fig 5a
Cell Signaling Technology Hist1h2aa antibody (Cell Signaling, 8240) was used in western blot on human samples (fig 5a). J Exp Clin Cancer Res (2019) ncbi
rabbit monoclonal (D27C4)
  • immunocytochemistry; mouse; 1:200; loading ...; fig s6d
Cell Signaling Technology Hist1h2aa antibody (Cell Signaling, 8240S) was used in immunocytochemistry on mouse samples at 1:200 (fig s6d). Cell (2019) ncbi
rabbit monoclonal (D27C4)
  • western blot; human; loading ...; fig 7b
Cell Signaling Technology Hist1h2aa antibody (Cell Signaling Technology, 8240) was used in western blot on human samples (fig 7b). Nat Cell Biol (2018) ncbi
rabbit monoclonal (D27C4)
  • ChIP-Seq; human; 1:100; loading ...; fig 6a
Cell Signaling Technology Hist1h2aa antibody (Cell Signaling, 8240) was used in ChIP-Seq on human samples at 1:100 (fig 6a). Nat Commun (2017) ncbi
rabbit monoclonal (D27C4)
  • immunocytochemistry; human; 1:1000; loading ...; fig 4e
  • western blot; human; 1:1000; loading ...; fig 4a
Cell Signaling Technology Hist1h2aa antibody (Cell Signaling Technology, 8240s) was used in immunocytochemistry on human samples at 1:1000 (fig 4e) and in western blot on human samples at 1:1000 (fig 4a). Sci Rep (2017) ncbi
rabbit monoclonal (D27C4)
  • western blot; human; loading ...; fig 5b
Cell Signaling Technology Hist1h2aa antibody (CST, 8240) was used in western blot on human samples (fig 5b). Sci Rep (2017) ncbi
rabbit monoclonal (D27C4)
  • chromatin immunoprecipitation; mouse; loading ...; tbl s3
Cell Signaling Technology Hist1h2aa antibody (Cell Signaling, 8240S) was used in chromatin immunoprecipitation on mouse samples (tbl s3). Science (2017) ncbi
rabbit monoclonal (D27C4)
  • ChIP-Seq; mouse; loading ...; fig 5a
  • immunohistochemistry; mouse; 1:200
  • western blot; mouse; loading ...; fig 5c
Cell Signaling Technology Hist1h2aa antibody (cell signaling, 8240) was used in ChIP-Seq on mouse samples (fig 5a), in immunohistochemistry on mouse samples at 1:200 and in western blot on mouse samples (fig 5c). Mol Cell (2017) ncbi
rabbit monoclonal (D27C4)
  • western blot; human; loading ...; fig 2a
Cell Signaling Technology Hist1h2aa antibody (Cell Signaling Technology, 8240) was used in western blot on human samples (fig 2a). J Biol Chem (2017) ncbi
rabbit monoclonal (D27C4)
  • immunocytochemistry; mouse; 1:500; fig 2
Cell Signaling Technology Hist1h2aa antibody (NEB, 8240) was used in immunocytochemistry on mouse samples at 1:500 (fig 2). Nat Commun (2016) ncbi
rabbit monoclonal (D27C4)
  • chromatin immunoprecipitation; fruit fly; 1:100; fig s10d
  • immunohistochemistry; fruit fly; 1:500; fig s10b
In order to demonstrate that PRC1 components act as neoplastic tumor suppressors independently of PRC2 function, Cell Signaling Technology Hist1h2aa antibody (Cell signaling, D27C4) was used in chromatin immunoprecipitation on fruit fly samples at 1:100 (fig s10d) and in immunohistochemistry on fruit fly samples at 1:500 (fig s10b). Nat Genet (2016) ncbi
rabbit monoclonal (D27C4)
  • chromatin immunoprecipitation; thale cress; fig 4
In order to study the interaction of LHP1 to repress KNOX genes in arabidopsis with transcription factors AS1 and AS2, Cell Signaling Technology Hist1h2aa antibody (Cell Signaling, D27C4) was used in chromatin immunoprecipitation on thale cress samples (fig 4). J Integr Plant Biol (2016) ncbi
rabbit monoclonal (D27C4)
  • chromatin immunoprecipitation; fruit fly; loading ...; fig s14c
Cell Signaling Technology Hist1h2aa antibody (Cell Signaling, 8240) was used in chromatin immunoprecipitation on fruit fly samples (fig s14c). Science (2016) ncbi
rabbit monoclonal (D27C4)
  • ChIP-Seq; mouse; fig 5
  • western blot; mouse; fig 5
Cell Signaling Technology Hist1h2aa antibody (Cell Signaling Technology, 8240S) was used in ChIP-Seq on mouse samples (fig 5) and in western blot on mouse samples (fig 5). PLoS ONE (2016) ncbi
rabbit monoclonal (D27C4)
  • ChIP-Seq; mouse; fig st2
  • western blot; mouse; fig s10
Cell Signaling Technology Hist1h2aa antibody (Cell Signaling, 8240) was used in ChIP-Seq on mouse samples (fig st2) and in western blot on mouse samples (fig s10). Epigenetics Chromatin (2016) ncbi
rabbit monoclonal (D27C4)
  • immunohistochemistry; mouse; 1:250; loading ...; fig 1b
In order to propose that non-canonical Polycomb Repressive Complex 1 is active during pre-implantation development, Cell Signaling Technology Hist1h2aa antibody (Cell Signaling, D27C4) was used in immunohistochemistry on mouse samples at 1:250 (fig 1b). Epigenetics (2016) ncbi
rabbit monoclonal (D27C4)
  • chromatin immunoprecipitation; mouse; fig 6
In order to study the importance of basal autophagy in the maintenance of the stem-cell quiescent state, Cell Signaling Technology Hist1h2aa antibody (Cell Signaling, 8240) was used in chromatin immunoprecipitation on mouse samples (fig 6). Nature (2016) ncbi
rabbit monoclonal (D27C4)
  • chromatin immunoprecipitation; mouse; fig 3
  • immunohistochemistry; mouse; 1:400; fig 4
  • western blot; mouse; 1:5000; fig 2
Cell Signaling Technology Hist1h2aa antibody (Cell Signaling, 8240) was used in chromatin immunoprecipitation on mouse samples (fig 3), in immunohistochemistry on mouse samples at 1:400 (fig 4) and in western blot on mouse samples at 1:5000 (fig 2). Oncotarget (2016) ncbi
rabbit monoclonal (D27C4)
  • immunohistochemistry - frozen section; mouse; 1:200; fig 2
  • chromatin immunoprecipitation; mouse; fig 2
Cell Signaling Technology Hist1h2aa antibody (Cell Signaling, 8240s) was used in immunohistochemistry - frozen section on mouse samples at 1:200 (fig 2) and in chromatin immunoprecipitation on mouse samples (fig 2). J Cell Biol (2016) ncbi
rabbit monoclonal (D27C4)
  • western blot; human; fig 5
Cell Signaling Technology Hist1h2aa antibody (Cell Signaling, D27C4) was used in western blot on human samples (fig 5). J Biol Chem (2015) ncbi
rabbit monoclonal (D27C4)
  • chromatin immunoprecipitation; human; fig s7
Cell Signaling Technology Hist1h2aa antibody (Cell Signaling, 8240) was used in chromatin immunoprecipitation on human samples (fig s7). Cell Res (2015) ncbi
rabbit monoclonal (D27C4)
In order to examine the association of the FBXL10/KDM2B scaffolding protein with novel polycomb repressive complex-1 and its role in adipogenesis, Cell Signaling Technology Hist1h2aa antibody (Cell Signaling, 8240) was used . J Biol Chem (2015) ncbi
rabbit monoclonal (D27C4)
  • western blot; human; fig 1i
  • chromatin immunoprecipitation; mouse; fig 1a
Cell Signaling Technology Hist1h2aa antibody (Cell Signaling Technology, 8240) was used in western blot on human samples (fig 1i) and in chromatin immunoprecipitation on mouse samples (fig 1a). Nature (2014) ncbi
rabbit monoclonal (D27C4)
  • western blot; human
Cell Signaling Technology Hist1h2aa antibody (Cell Signaling Technology, 8240) was used in western blot on human samples . J Biol Chem (2014) ncbi
rabbit monoclonal (D27C4)
  • western blot; human
Cell Signaling Technology Hist1h2aa antibody (Cell Signaling, 8240) was used in western blot on human samples . elife (2014) ncbi
rabbit monoclonal (D27C4)
  • immunocytochemistry; mouse; 1:500; fig 1
  • western blot; mouse; 1:1000; fig s1c
Cell Signaling Technology Hist1h2aa antibody (Cell Signaling Technology, 8240) was used in immunocytochemistry on mouse samples at 1:500 (fig 1) and in western blot on mouse samples at 1:1000 (fig s1c). Cell Rep (2014) ncbi
rabbit monoclonal (D27C4)
  • chromatin immunoprecipitation; human
  • western blot; human; 1:1000
In order to study the Involvement of EED in the organization of polycomb group complexes, Cell Signaling Technology Hist1h2aa antibody (Cell Signaling, 8240) was used in chromatin immunoprecipitation on human samples and in western blot on human samples at 1:1000. Nat Commun (2014) ncbi
rabbit monoclonal (D27C4)
  • immunoprecipitation; human
Cell Signaling Technology Hist1h2aa antibody (CST, 8240s) was used in immunoprecipitation on human samples . J Biol Chem (2013) ncbi
rabbit monoclonal (D27C4)
  • western blot; mouse
In order to study the role of polycomb repressive complex 2 in leukemia progression, Cell Signaling Technology Hist1h2aa antibody (Cell Signaling, D27C4) was used in western blot on mouse samples . Blood (2013) ncbi
Articles Reviewed
  1. Traynor S, Møllegaard N, Jørgensen M, Brückmann N, Pedersen C, Terp M, et al. Remodeling and destabilization of chromosome 1 pericentromeric heterochromatin by SSX proteins. Nucleic Acids Res. 2019;47:6668-6684 pubmed publisher
  2. Fursova N, Blackledge N, Nakayama M, Ito S, Koseki Y, Farcas A, et al. Synergy between Variant PRC1 Complexes Defines Polycomb-Mediated Gene Repression. Mol Cell. 2019;74:1020-1036.e8 pubmed publisher
  3. He M, Chaurushiya M, Webster J, Kummerfeld S, Reja R, Chaudhuri S, et al. Intrinsic apoptosis shapes the tumor spectrum linked to inactivation of the deubiquitinase BAP1. Science. 2019;364:283-285 pubmed publisher
  4. Lavarone E, Barbieri C, Pasini D. Dissecting the role of H3K27 acetylation and methylation in PRC2 mediated control of cellular identity. Nat Commun. 2019;10:1679 pubmed publisher
  5. Kelly M, So J, Rogers A, Gregory G, Li J, Zethoven M, et al. Bcor loss perturbs myeloid differentiation and promotes leukaemogenesis. Nat Commun. 2019;10:1347 pubmed publisher
  6. Chen X, Hu L, Yang H, Ma H, Ye K, Zhao C, et al. DHHC protein family targets different subsets of glioma stem cells in specific niches. J Exp Clin Cancer Res. 2019;38:25 pubmed publisher
  7. Żylicz J, Bousard A, Zumer K, Dossin F, Mohammad E, da Rocha S, et al. The Implication of Early Chromatin Changes in X Chromosome Inactivation. Cell. 2019;176:182-197.e23 pubmed publisher
  8. Zhang Y, Shi J, Liu X, Feng L, Gong Z, Koppula P, et al. BAP1 links metabolic regulation of ferroptosis to tumour suppression. Nat Cell Biol. 2018;20:1181-1192 pubmed publisher
  9. Ning B, Zhao W, Qian C, Liu P, Li Q, Li W, et al. USP26 functions as a negative regulator of cellular reprogramming by stabilising PRC1 complex components. Nat Commun. 2017;8:349 pubmed publisher
  10. Patel N, Garikapati K, Pandita R, Singh D, Pandita T, Bhadra U, et al. miR-15a/miR-16 down-regulates BMI1, impacting Ub-H2A mediated DNA repair and breast cancer cell sensitivity to doxorubicin. Sci Rep. 2017;7:4263 pubmed publisher
  11. Yan Y, Zhao W, Huang Y, Tong H, Xia Y, Jiang Q, et al. Loss of Polycomb Group Protein Pcgf1 Severely Compromises Proper Differentiation of Embryonic Stem Cells. Sci Rep. 2017;7:46276 pubmed publisher
  12. Lau M, Schwartz M, Kundu S, Savol A, Wang P, Marr S, et al. Mutation of a nucleosome compaction region disrupts Polycomb-mediated axial patterning. Science. 2017;355:1081-1084 pubmed publisher
  13. Jullien J, Vodnala M, Pasque V, Oikawa M, Miyamoto K, Allen G, et al. Gene Resistance to Transcriptional Reprogramming following Nuclear Transfer Is Directly Mediated by Multiple Chromatin-Repressive Pathways. Mol Cell. 2017;65:873-884.e8 pubmed publisher
  14. Wheaton K, Sarkari F, Stanly Johns B, Davarinejad H, Egorova O, Kaustov L, et al. UbE2E1/UBCH6 Is a Critical in Vivo E2 for the PRC1-catalyzed Ubiquitination of H2A at Lys-119. J Biol Chem. 2017;292:2893-2902 pubmed publisher
  15. Cooper S, Grijzenhout A, Underwood E, Ancelin K, Zhang T, Nesterova T, et al. Jarid2 binds mono-ubiquitylated H2A lysine 119 to mediate crosstalk between Polycomb complexes PRC1 and PRC2. Nat Commun. 2016;7:13661 pubmed publisher
  16. Loubiere V, Delest A, Thomas A, Bonev B, Schuettengruber B, Sati S, et al. Coordinate redeployment of PRC1 proteins suppresses tumor formation during Drosophila development. Nat Genet. 2016;48:1436-1442 pubmed publisher
  17. Li Z, Li B, Liu J, Guo Z, Liu Y, Li Y, et al. Transcription factors AS1 and AS2 interact with LHP1 to repress KNOX genes in Arabidopsis. J Integr Plant Biol. 2016;58:959-970 pubmed publisher
  18. Lu C, Jain S, Hoelper D, Bechet D, Molden R, Ran L, et al. Histone H3K36 mutations promote sarcomagenesis through altered histone methylation landscape. Science. 2016;352:844-9 pubmed publisher
  19. Si S, Nakajima Takagi Y, Aoyama K, Oshima M, Saraya A, Sugishita H, et al. Loss of Pcgf5 Affects Global H2A Monoubiquitination but Not the Function of Hematopoietic Stem and Progenitor Cells. PLoS ONE. 2016;11:e0154561 pubmed publisher
  20. Stelloh C, Reimer M, Pulakanti K, Blinka S, Peterson J, Pinello L, et al. The cohesin-associated protein Wapal is required for proper Polycomb-mediated gene silencing. Epigenetics Chromatin. 2016;9:14 pubmed publisher
  21. Eid A, Torres Padilla M. Characterization of non-canonical Polycomb Repressive Complex 1 subunits during early mouse embryogenesis. Epigenetics. 2016;11:389-97 pubmed publisher
  22. García Prat L, Martínez Vicente M, Perdiguero E, Ortet L, Rodríguez Ubreva J, Rebollo E, et al. Autophagy maintains stemness by preventing senescence. Nature. 2016;529:37-42 pubmed publisher
  23. Benitz S, Regel I, Reinhard T, Popp A, Schäffer I, Raulefs S, et al. Polycomb repressor complex 1 promotes gene silencing through H2AK119 mono-ubiquitination in acinar-to-ductal metaplasia and pancreatic cancer cells. Oncotarget. 2016;7:11424-33 pubmed publisher
  24. Mardaryev A, Liu B, Rapisarda V, Poterlowicz K, Malashchuk I, Rudolf J, et al. Cbx4 maintains the epithelial lineage identity and cell proliferation in the developing stratified epithelium. J Cell Biol. 2016;212:77-89 pubmed publisher
  25. Daou S, Hammond Martel I, Mashtalir N, Barbour H, Gagnon J, Iannantuono N, et al. The BAP1/ASXL2 Histone H2A Deubiquitinase Complex Regulates Cell Proliferation and Is Disrupted in Cancer. J Biol Chem. 2015;290:28643-63 pubmed publisher
  26. Papadakis A, Sun C, Knijnenburg T, Xue Y, Grernrum W, Hölzel M, et al. SMARCE1 suppresses EGFR expression and controls responses to MET and ALK inhibitors in lung cancer. Cell Res. 2015;25:445-58 pubmed publisher
  27. Inagaki T, Iwasaki S, Matsumura Y, Kawamura T, Tanaka T, Abe Y, et al. The FBXL10/KDM2B scaffolding protein associates with novel polycomb repressive complex-1 to regulate adipogenesis. J Biol Chem. 2015;290:4163-77 pubmed publisher
  28. Bhatnagar S, Gazin C, Chamberlain L, Ou J, Zhu X, Tushir J, et al. TRIM37 is a new histone H2A ubiquitin ligase and breast cancer oncoprotein. Nature. 2014;516:116-20 pubmed publisher
  29. Hock A, Vigneron A, Vousden K. Ubiquitin-specific peptidase 42 (USP42) functions to deubiquitylate histones and regulate transcriptional activity. J Biol Chem. 2014;289:34862-70 pubmed publisher
  30. Ji X, Lu H, Zhou Q, Luo K. LARP7 suppresses P-TEFb activity to inhibit breast cancer progression and metastasis. elife. 2014;3:e02907 pubmed publisher
  31. Cooper S, Dienstbier M, Hassan R, Schermelleh L, Sharif J, Blackledge N, et al. Targeting polycomb to pericentric heterochromatin in embryonic stem cells reveals a role for H2AK119u1 in PRC2 recruitment. Cell Rep. 2014;7:1456-1470 pubmed publisher
  32. Cao Q, Wang X, Zhao M, Yang R, Malik R, Qiao Y, et al. The central role of EED in the orchestration of polycomb group complexes. Nat Commun. 2014;5:3127 pubmed publisher
  33. Yuan G, Ma B, Yuan W, Zhang Z, Chen P, Ding X, et al. Histone H2A ubiquitination inhibits the enzymatic activity of H3 lysine 36 methyltransferases. J Biol Chem. 2013;288:30832-42 pubmed publisher
  34. Lee S, Phipson B, Hyland C, Leong H, Allan R, Lun A, et al. Polycomb repressive complex 2 (PRC2) suppresses E?-myc lymphoma. Blood. 2013;122:2654-63 pubmed publisher