This is a Validated Antibody Database (VAD) review about rat Herc2, based on 5 published articles (read how Labome selects the articles), using Herc2 antibody in all methods. It is aimed to help Labome visitors find the most suited Herc2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Herc2 synonym: E3 ubiquitin-protein ligase HERC2; hect (homologous to the E6-AP (UBE3A) carboxyl terminus) domain and RCC1 (CHC1)-like domain (RLD) 2; hect domain and RLD 2

BD Biosciences
mouse monoclonal (17/HERC2)
  • immunoprecipitation; human; fig 3
  • western blot; human; fig 3
BD Biosciences Herc2 antibody (BD Transduction Labs, 612366) was used in immunoprecipitation on human samples (fig 3) and in western blot on human samples (fig 3). elife (2015) ncbi
mouse monoclonal (17/HERC2)
  • immunocytochemistry; human; 1:100; fig 1f
  • western blot; human; 1:500; fig 1c
  • western blot; mouse; 1:500; fig 1d
In order to analyze protein kinase LRRK2, parkinson's disease associated, which modulates notch signaling via the endosomal pathway, BD Biosciences Herc2 antibody (BD Biosciences, 612366) was used in immunocytochemistry on human samples at 1:100 (fig 1f), in western blot on human samples at 1:500 (fig 1c) and in western blot on mouse samples at 1:500 (fig 1d). PLoS Genet (2015) ncbi
mouse monoclonal (17/HERC2)
  • western blot; human; fig 1
In order to elucidate how microphthalmia-associated transcription factor regulates transcription, BD Biosciences Herc2 antibody (BD Transduction Laboratories, 612366) was used in western blot on human samples (fig 1). elife (2015) ncbi
mouse monoclonal (17/HERC2)
  • western blot; human
BD Biosciences Herc2 antibody (BD, 612366) was used in western blot on human samples . Cancer Res (2015) ncbi
mouse monoclonal (17/HERC2)
  • western blot; human; 1:1000
BD Biosciences Herc2 antibody (BD, 612366) was used in western blot on human samples at 1:1000. Nature (2014) ncbi
Articles Reviewed
  1. Mancias J, Pontano Vaites L, Nissim S, Biancur D, Kim A, Wang X, et al. Ferritinophagy via NCOA4 is required for erythropoiesis and is regulated by iron dependent HERC2-mediated proteolysis. elife. 2015;4: pubmed publisher
  2. Imai Y, Kobayashi Y, Inoshita T, Meng H, Arano T, Uemura K, et al. The Parkinson's Disease-Associated Protein Kinase LRRK2 Modulates Notch Signaling through the Endosomal Pathway. PLoS Genet. 2015;11:e1005503 pubmed publisher
  3. Laurette P, Strub T, Koludrovic D, Keime C, Le Gras S, Seberg H, et al. Transcription factor MITF and remodeller BRG1 define chromatin organisation at regulatory elements in melanoma cells. elife. 2015;4: pubmed publisher
  4. Peng Y, Dai H, Wang E, Lin C, Mo W, Peng G, et al. TUSC4 functions as a tumor suppressor by regulating BRCA1 stability. Cancer Res. 2015;75:378-86 pubmed publisher
  5. Mancias J, Wang X, Gygi S, Harper J, Kimmelman A. Quantitative proteomics identifies NCOA4 as the cargo receptor mediating ferritinophagy. Nature. 2014;509:105-9 pubmed publisher