This is a Validated Antibody Database (VAD) review about rat Grm5, based on 28 published articles (read how Labome selects the articles), using Grm5 antibody in all methods. It is aimed to help Labome visitors find the most suited Grm5 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Grm5 synonym: mGluR5; mGlur5; metabotropic glutamate receptor 5; glutamate receptor, metabotropic 5; metabotropic glutamate receptor (mGluR5); metabotropic glutamate receptor 5b

Abcam
rabbit monoclonal (EPR2425Y)
  • western blot; mouse; loading ...; tbl 3
In order to investigate the neural mechanism underlying nicotine withdrawal-induced inattention, Abcam Grm5 antibody (Abcam, ab76316) was used in western blot on mouse samples (tbl 3). Psychopharmacology (Berl) (2017) ncbi
rabbit monoclonal (EPR2425Y)
  • western blot; mouse; fig 2
In order to examine the developmental requirement of Shank3, Abcam Grm5 antibody (Abcam, ab76316) was used in western blot on mouse samples (fig 2). Nature (2016) ncbi
rabbit monoclonal (EPR2425Y)
  • immunocytochemistry; mouse; 1:250; fig s8
In order to study the coupling of cellular prion protein to intracellular signaling in Alzheimer's disease by metabotropic glutamate receptor 5, Abcam Grm5 antibody (Abcam, ab76316) was used in immunocytochemistry on mouse samples at 1:250 (fig s8). Brain (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 1
Abcam Grm5 antibody (ABCAM, ab27190) was used in western blot on human samples (fig 1). Acta Neuropathol (2015) ncbi
rabbit monoclonal (EPR2425Y)
  • western blot; rat; 1:1000
Abcam Grm5 antibody (Abcam, ab76316) was used in western blot on rat samples at 1:1000. Mol Psychiatry (2015) ncbi
rabbit polyclonal
  • western blot; mouse; 1:300
In order to examine how glutamate modulates intestinal epithelial cell growth and epithelial barrier function during total parenteral nutrition, Abcam Grm5 antibody (Abcam, ab-53090) was used in western blot on mouse samples at 1:300. FASEB J (2014) ncbi
rabbit polyclonal
  • western blot; human; 1:300
Abcam Grm5 antibody (Abcam, ab53090) was used in western blot on human samples at 1:300. Transl Psychiatry (2013) ncbi
rabbit polyclonal
  • western blot; rat; 1:500
  • immunohistochemistry - paraffin section; human; 1:100
Abcam Grm5 antibody (Abcam, ab53090) was used in western blot on rat samples at 1:500 and in immunohistochemistry - paraffin section on human samples at 1:100. Glia (2013) ncbi
rabbit polyclonal
  • immunocytochemistry; mouse; 1:100
Abcam Grm5 antibody (Abcam, ab53090) was used in immunocytochemistry on mouse samples at 1:100. Cell J (2012) ncbi
rabbit polyclonal
  • western blot; human; 1:250
Abcam Grm5 antibody (Abcam, ab27190) was used in western blot on human samples at 1:250. Schizophr Res (2013) ncbi
Invitrogen
rabbit polyclonal
  • western blot; mouse; 1:500; fig 3
In order to determine if the hippocampal gene/protein expression of relevant glutamate signaling components, cannabinoid type 1 receptor, and endocannabinoid metabolic enzymes were altered following acute and/or repeated cocaine administration, Invitrogen Grm5 antibody (Thermo Scientific, PA1-24637) was used in western blot on mouse samples at 1:500 (fig 3). Eur Neuropsychopharmacol (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - free floating section; rat; 1:1000
In order to test the effect of a p38alpha MAPK inhibitor on spinal cord injury in the rat, Invitrogen Grm5 antibody (ThermoScientific, PA1-4663) was used in immunohistochemistry - free floating section on rat samples at 1:1000. Pain (2014) ncbi
Alomone Labs
rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; fig 7
  • immunoprecipitation; mouse; fig 7
  • immunocytochemistry; mouse; fig s7
  • western blot; mouse; fig 6
Alomone Labs Grm5 antibody (Alomone Labs, AGC-007) was used in immunohistochemistry - frozen section on mouse samples (fig 7), in immunoprecipitation on mouse samples (fig 7), in immunocytochemistry on mouse samples (fig s7) and in western blot on mouse samples (fig 6). Nat Commun (2016) ncbi
EMD Millipore
rabbit polyclonal
  • western blot; mouse; 1:3000; loading ...; fig 1
EMD Millipore Grm5 antibody (Millipore, AB5675) was used in western blot on mouse samples at 1:3000 (fig 1). Mol Pharmacol (2018) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 1d
EMD Millipore Grm5 antibody (Millipore, ab5675) was used in western blot on mouse samples at 1:1000 (fig 1d). J Neurochem (2018) ncbi
rabbit polyclonal
  • immunocytochemistry; mouse; 1:50; fig 2h
In order to study metabotropic glutamate receptor 5 in a murine model of fragile X syndrome, EMD Millipore Grm5 antibody (Millipore, AB5675) was used in immunocytochemistry on mouse samples at 1:50 (fig 2h). Dev Neurobiol (2017) ncbi
rabbit polyclonal
  • western blot; rat; fig 5
In order to characterize and identify PSD-95 depalmitoylating enzymes, EMD Millipore Grm5 antibody (EMD Millipore, AB5675) was used in western blot on rat samples (fig 5). J Neurosci (2016) ncbi
rabbit polyclonal
  • western blot; mouse; loading ...; fig 2a
In order to present the effect of pharmacological enhancement of mGlu5 receptors on behavioral deficits in SHANK3 knock-out mice, EMD Millipore Grm5 antibody (Millipore, AB5675) was used in western blot on mouse samples (fig 2a). Mol Psychiatry (2017) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000; fig 7a
In order to show how ATL1 and VCP work together to regulate endoplasmic reticulum and protein synthesis for dendritic spine formation, EMD Millipore Grm5 antibody (Millipore, AB5675) was used in western blot on mouse samples at 1:1000 (fig 7a). Nat Commun (2016) ncbi
rabbit polyclonal
  • other; rat; 1:400; fig 1
  • immunohistochemistry - free floating section; rat; 1:500; fig s2
  • immunocytochemistry; rat; 1:250; fig 1
  • western blot; rat; 1:1000; fig 1
In order to elucidate neuropathic pain and the critical role of intracellular mGluR5, EMD Millipore Grm5 antibody (Millipore, AB5675) was used in other on rat samples at 1:400 (fig 1), in immunohistochemistry - free floating section on rat samples at 1:500 (fig s2), in immunocytochemistry on rat samples at 1:250 (fig 1) and in western blot on rat samples at 1:1000 (fig 1). Nat Commun (2016) ncbi
rabbit polyclonal
  • western blot; mouse; fig 5a
EMD Millipore Grm5 antibody (Millipore, AB5675) was used in western blot on mouse samples (fig 5a). Sci Rep (2015) ncbi
rabbit polyclonal
  • western blot; rat; 1:250
EMD Millipore Grm5 antibody (Millipore, AB5675) was used in western blot on rat samples at 1:250. Front Cell Neurosci (2015) ncbi
mouse monoclonal (N75/33)
  • immunohistochemistry - paraffin section; human; 1:100
EMD Millipore Grm5 antibody (EMD Millipore, MABN540) was used in immunohistochemistry - paraffin section on human samples at 1:100. J Child Neurol (2016) ncbi
rabbit polyclonal
  • western blot; mouse; fig 7
EMD Millipore Grm5 antibody (Millipore, AB5675) was used in western blot on mouse samples (fig 7). J Neurosci (2015) ncbi
rabbit polyclonal
  • western blot; rat
In order to determine gene expression during the initial, intermediate, and later stages of ischemia/reperfusion injury, EMD Millipore Grm5 antibody (EMD Millipore, AB5675) was used in western blot on rat samples . Ophthalmol Eye Dis (2014) ncbi
rabbit polyclonal
  • western blot; rat; 1:2000
EMD Millipore Grm5 antibody (Millipore, AB5675) was used in western blot on rat samples at 1:2000. PLoS ONE (2014) ncbi
rabbit polyclonal
  • western blot; mouse; fig 4
EMD Millipore Grm5 antibody (Millipore, 5675) was used in western blot on mouse samples (fig 4). EMBO Mol Med (2014) ncbi
Neuromab
mouse monoclonal (N75/33)
  • western blot; mouse; fig 7
In order to analyze selective partitioning into complexes and supercomplexes during synapse maturation by NMDA receptors, Neuromab Grm5 antibody (NeuroMab, 75-116) was used in western blot on mouse samples (fig 7). Nat Commun (2016) ncbi
Articles Reviewed
  1. Marks C, Shonesy B, Wang X, Stephenson J, Niswender C, Colbran R. Activated CaMKIIα Binds to the mGlu5 Metabotropic Glutamate Receptor and Modulates Calcium Mobilization. Mol Pharmacol. 2018;94:1352-1362 pubmed publisher
  2. Lautz J, Brown E, Williams VanSchoiack A, Smith S. Synaptic activity induces input-specific rearrangements in a targeted synaptic protein interaction network. J Neurochem. 2018;146:540-559 pubmed publisher
  3. Higa K, Grim A, Kamenski M, van Enkhuizen J, Zhou X, Li K, et al. Nicotine withdrawal-induced inattention is absent in alpha7 nAChR knockout mice. Psychopharmacology (Berl). 2017;234:1573-1586 pubmed publisher
  4. Achuta V, Grym H, Putkonen N, Louhivuori V, Kärkkäinen V, Koistinaho J, et al. Metabotropic glutamate receptor 5 responses dictate differentiation of neural progenitors to NMDA-responsive cells in fragile X syndrome. Dev Neurobiol. 2017;77:438-453 pubmed publisher
  5. Yokoi N, Fukata Y, Sekiya A, Murakami T, Kobayashi K, Fukata M. Identification of PSD-95 Depalmitoylating Enzymes. J Neurosci. 2016;36:6431-44 pubmed publisher
  6. Wang X, Bey A, Katz B, Badea A, Kim N, David L, et al. Altered mGluR5-Homer scaffolds and corticostriatal connectivity in a Shank3 complete knockout model of autism. Nat Commun. 2016;7:11459 pubmed publisher
  7. Frank R, Komiyama N, Ryan T, Zhu F, O Dell T, Grant S. NMDA receptors are selectively partitioned into complexes and supercomplexes during synapse maturation. Nat Commun. 2016;7:11264 pubmed publisher
  8. Vicidomini C, Ponzoni L, Lim D, Schmeisser M, Reim D, Morello N, et al. Pharmacological enhancement of mGlu5 receptors rescues behavioral deficits in SHANK3 knock-out mice. Mol Psychiatry. 2017;22:689-702 pubmed publisher
  9. Shih Y, Hsueh Y. VCP and ATL1 regulate endoplasmic reticulum and protein synthesis for dendritic spine formation. Nat Commun. 2016;7:11020 pubmed publisher
  10. Mei Y, Monteiro P, Zhou Y, Kim J, Gao X, Fu Z, et al. Adult restoration of Shank3 expression rescues selective autistic-like phenotypes. Nature. 2016;530:481-4 pubmed publisher
  11. Vincent K, Cornea V, Jong Y, Laferrière A, Kumar N, Mickeviciute A, et al. Intracellular mGluR5 plays a critical role in neuropathic pain. Nat Commun. 2016;7:10604 pubmed publisher
  12. Blanco E, Galeano P, Palomino A, Pavón F, Rivera P, Serrano A, et al. Cocaine-induced behavioral sensitization decreases the expression of endocannabinoid signaling-related proteins in the mouse hippocampus. Eur Neuropsychopharmacol. 2016;26:477-92 pubmed publisher
  13. Haas L, Salazar S, Kostylev M, Um J, Kaufman A, Strittmatter S. Metabotropic glutamate receptor 5 couples cellular prion protein to intracellular signalling in Alzheimer's disease. Brain. 2016;139:526-46 pubmed publisher
  14. Hatanaka Y, Watase K, Wada K, Nagai Y. Abnormalities in synaptic dynamics during development in a mouse model of spinocerebellar ataxia type 1. Sci Rep. 2015;5:16102 pubmed publisher
  15. Bragina L, Bonifacino T, Bassi S, Milanese M, Bonanno G, Conti F. Differential expression of metabotropic glutamate and GABA receptors at neocortical glutamatergic and GABAergic axon terminals. Front Cell Neurosci. 2015;9:345 pubmed publisher
  16. Lechpammer M, Wintermark P, Merry K, Jackson M, Jantzie L, Jensen F. Dysregulation of FMRP/mTOR Signaling Cascade in Hypoxic-Ischemic Injury of Premature Human Brain. J Child Neurol. 2016;31:426-32 pubmed publisher
  17. Matosin N, Fernandez Enright F, Fung S, Lum J, Engel M, Andrews J, et al. Alterations of mGluR5 and its endogenous regulators Norbin, Tamalin and Preso1 in schizophrenia: towards a model of mGluR5 dysregulation. Acta Neuropathol. 2015;130:119-29 pubmed publisher
  18. Lo S, Wang Y, Weber M, Larson J, Scearce Levie K, Sheng M. Caspase-3 deficiency results in disrupted synaptic homeostasis and impaired attention control. J Neurosci. 2015;35:2118-32 pubmed publisher
  19. Ledonne A, Nobili A, Latagliata E, Cavallucci V, Guatteo E, Puglisi Allegra S, et al. Neuregulin 1 signalling modulates mGluR1 function in mesencephalic dopaminergic neurons. Mol Psychiatry. 2015;20:959-73 pubmed publisher
  20. Andreeva K, Zhang M, Fan W, Li X, Chen Y, Rebolledo Mendez J, et al. Time-dependent Gene Profiling Indicates the Presence of Different Phases for Ischemia/Reperfusion Injury in Retina. Ophthalmol Eye Dis. 2014;6:43-54 pubmed publisher
  21. Galán Arriero I, Avila Martin G, Ferrer Donato A, Gómez Soriano J, Bravo Esteban E, Taylor J. Oral administration of the p38α MAPK inhibitor, UR13870, inhibits affective pain behavior after spinal cord injury. Pain. 2014;155:2188-98 pubmed publisher
  22. Gong K, Kung L, Magni G, Bhargava A, Jasmin L. Increased response to glutamate in small diameter dorsal root ganglion neurons after sciatic nerve injury. PLoS ONE. 2014;9:e95491 pubmed publisher
  23. Xiao W, Feng Y, Holst J, Hartmann B, Yang H, Teitelbaum D. Glutamate prevents intestinal atrophy via luminal nutrient sensing in a mouse model of total parenteral nutrition. FASEB J. 2014;28:2073-87 pubmed publisher
  24. Arroyo A, Camoletto P, Morando L, Sassoè Pognetto M, Giustetto M, Van Veldhoven P, et al. Pharmacological reversion of sphingomyelin-induced dendritic spine anomalies in a Niemann Pick disease type A mouse model. EMBO Mol Med. 2014;6:398-413 pubmed publisher
  25. Fatemi S, Folsom T, Rooney R, Thuras P. mRNA and protein expression for novel GABAA receptors ? and ?2 are altered in schizophrenia and mood disorders; relevance to FMRP-mGluR5 signaling pathway. Transl Psychiatry. 2013;3:e271 pubmed publisher
  26. Lim D, Iyer A, Ronco V, Grolla A, Canonico P, Aronica E, et al. Amyloid beta deregulates astroglial mGluR5-mediated calcium signaling via calcineurin and Nf-kB. Glia. 2013;61:1134-45 pubmed publisher
  27. Meamar R, Karamali F, Mousavi S, Baharvand H, Nasr Esfahani M. Could MDMA Promote Stemness Characteristics in Mouse Embryonic Stem Cells via mGlu5 Metabotropic Glutamate Receptors?. Cell J. 2012;14:185-92 pubmed
  28. Matosin N, Frank E, Deng C, Huang X, Newell K. Metabotropic glutamate receptor 5 binding and protein expression in schizophrenia and following antipsychotic drug treatment. Schizophr Res. 2013;146:170-6 pubmed publisher