This is a Validated Antibody Database (VAD) review about rat Gpx1, based on 25 published articles (read how Labome selects the articles), using Gpx1 antibody in all methods. It is aimed to help Labome visitors find the most suited Gpx1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Gpx1 synonym: GSHPx; GSHPx-1; glutathione peroxidase 1; GPx-1; cellular glutathione peroxidase

Abcam
rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:200; loading ...; fig 5e, 5s2c
Abcam Gpx1 antibody (Abcam, ab22604) was used in immunohistochemistry - paraffin section on human samples at 1:200 (fig 5e, 5s2c). elife (2019) ncbi
rabbit polyclonal
  • western blot; mouse; fig 6d
Abcam Gpx1 antibody (Abcam, Ab22604) was used in western blot on mouse samples (fig 6d). Cell (2018) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; human; loading ...; fig 5c
  • western blot; human; 1:100; loading ...; fig 5a
Abcam Gpx1 antibody (Abcam, ab22604) was used in immunohistochemistry - paraffin section on human samples (fig 5c) and in western blot on human samples at 1:100 (fig 5a). Biochim Biophys Acta Proteins Proteom (2017) ncbi
rabbit polyclonal
  • western blot; human; fig 2a
Abcam Gpx1 antibody (Abcam, ab22604) was used in western blot on human samples (fig 2a). Placenta (2017) ncbi
rabbit monoclonal (EPR3312)
  • western blot; human; 1:1000; loading ...; fig 3a
Abcam Gpx1 antibody (Abcam, ab108427) was used in western blot on human samples at 1:1000 (fig 3a). Front Physiol (2016) ncbi
rabbit polyclonal
  • western blot; rat; fig 5a
Abcam Gpx1 antibody (Abcam, ab22604) was used in western blot on rat samples (fig 5a). PLoS ONE (2016) ncbi
rabbit polyclonal
  • western blot; human; loading ...; fig 6a
In order to examine the changes to heat shock proteins after blood flow restricted exercise, Abcam Gpx1 antibody (Abcam, ab22604) was used in western blot on human samples (fig 6a). Scand J Med Sci Sports (2017) ncbi
rabbit polyclonal
  • western blot; mouse; fig 6
Abcam Gpx1 antibody (Abcam, ab22604) was used in western blot on mouse samples (fig 6). Redox Biol (2016) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000; fig 3
Abcam Gpx1 antibody (Abcam, ab22604) was used in western blot on mouse samples at 1:1000 (fig 3). PLoS ONE (2016) ncbi
rabbit polyclonal
  • western blot; human; 1:1000; fig 4b
Abcam Gpx1 antibody (Abcam, ab22604) was used in western blot on human samples at 1:1000 (fig 4b). Mol Med Rep (2016) ncbi
rabbit polyclonal
  • western blot; mouse
Abcam Gpx1 antibody (Abcam, 22604) was used in western blot on mouse samples . PLoS ONE (2015) ncbi
rabbit monoclonal (EPR3311)
  • western blot; human; 1:1000
In order to measure nuclear and cytoplasmic protein abundances in human IMR90 fibroblasts subjected to mild oxidative stress using a subcellular spatial razor approach, Abcam Gpx1 antibody (Abcam, ab108429) was used in western blot on human samples at 1:1000. J Proteome Res (2014) ncbi
rabbit polyclonal
  • western blot; rat; 1:1000
Abcam Gpx1 antibody (Abcam, ab22604) was used in western blot on rat samples at 1:1000. Sleep (2014) ncbi
rabbit polyclonal
  • western blot; rat; 1:2000
Abcam Gpx1 antibody (Abcam, ab22604) was used in western blot on rat samples at 1:2000. PLoS ONE (2014) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000
Abcam Gpx1 antibody (Abcam, ab22604) was used in western blot on mouse samples at 1:1000. Exp Eye Res (2013) ncbi
Santa Cruz Biotechnology
mouse monoclonal (E-7)
  • western blot; mouse; 1:500; loading ...; fig 5c
In order to propose that long-term quercetin consumption benefits the dystrophic heart, Santa Cruz Biotechnology Gpx1 antibody (Santa Cruz Biotechnology, sc-74498) was used in western blot on mouse samples at 1:500 (fig 5c). Am J Physiol Heart Circ Physiol (2017) ncbi
mouse monoclonal (B-6)
  • western blot; human; loading ...; fig 9a
In order to present the effect thiosemicarbazones and inhibitors of thioredoxin reductase on mitochondrial peroxide defense, Santa Cruz Biotechnology Gpx1 antibody (Santa Cruz, sc-133160) was used in western blot on human samples (fig 9a). Free Radic Biol Med (2016) ncbi
mouse monoclonal (E-7)
  • western blot; human; 1:300; fig s4
Santa Cruz Biotechnology Gpx1 antibody (Santa Cruz, sc-74498) was used in western blot on human samples at 1:300 (fig s4). Redox Biol (2015) ncbi
mouse monoclonal (D-12)
  • western blot; mouse
In order to study the mechanism for 2-hydroxyethyl methacrylate-induced apoptosis, Santa Cruz Biotechnology Gpx1 antibody (Santa Cruz, sc-133152) was used in western blot on mouse samples . Biomaterials (2014) ncbi
mouse monoclonal (B-6)
  • western blot; human
Santa Cruz Biotechnology Gpx1 antibody (Santa Cruz Biotechnology, sc-133160) was used in western blot on human samples . J Biol Chem (2012) ncbi
R&D Systems
goat polyclonal
  • western blot; human; 1:1000; fig s14a
R&D Systems Gpx1 antibody (R&D Systems, AF3798) was used in western blot on human samples at 1:1000 (fig s14a). Nat Chem Biol (2016) ncbi
goat polyclonal
  • western blot; mouse; 1:100
R&D Systems Gpx1 antibody (R&D Systems, AF3798) was used in western blot on mouse samples at 1:100. Free Radic Biol Med (2015) ncbi
Invitrogen
rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 4d
In order to discuss mechanisms associated with platelet-derived growth factor, subtype BB protection against oxidative damage, Invitrogen Gpx1 antibody (Thermo Scientific, PA5-30593) was used in western blot on human samples at 1:1000 (fig 4d). Mol Neurobiol (2018) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 4e
In order to explore the links between oxidative perturbations and plasma albumin modifications in the liver, Invitrogen Gpx1 antibody (Pierce, PA5-30593) was used in western blot on mouse samples at 1:1000 (fig 4e). Free Radic Biol Med (2017) ncbi
GeneTex
rabbit polyclonal
  • western blot; mouse; fig 3
GeneTex Gpx1 antibody (GeneTex, GTX116040) was used in western blot on mouse samples (fig 3). PLoS ONE (2016) ncbi
Articles Reviewed
  1. Basnet H, Tian L, Ganesh K, Huang Y, Macalinao D, Brogi E, et al. Flura-seq identifies organ-specific metabolic adaptations during early metastatic colonization. elife. 2019;8: pubmed publisher
  2. Ingold I, Berndt C, Schmitt S, Doll S, Poschmann G, Buday K, et al. Selenium Utilization by GPX4 Is Required to Prevent Hydroperoxide-Induced Ferroptosis. Cell. 2018;172:409-422.e21 pubmed publisher
  3. Jin X, Xu Z, Cao J, Shao P, Zhou M, Qin Z, et al. Proteomics analysis of human placenta reveals glutathione metabolism dysfunction as the underlying pathogenesis for preeclampsia. Biochim Biophys Acta Proteins Proteom. 2017;1865:1207-1214 pubmed publisher
  4. Li M, Cheng W, Luo J, Hu X, Nie T, Lai H, et al. Loss of selenocysteine insertion sequence binding protein 2 suppresses the proliferation, migration/invasion and hormone secretion of human trophoblast cells via the PI3K/Akt and ERK signaling pathway. Placenta. 2017;55:81-89 pubmed publisher
  5. Cabezas R, Vega Vela N, González Sanmiguel J, Gonzalez J, Esquinas P, Echeverria V, et al. PDGF-BB Preserves Mitochondrial Morphology, Attenuates ROS Production, and Upregulates Neuroglobin in an Astrocytic Model Under Rotenone Insult. Mol Neurobiol. 2018;55:3085-3095 pubmed publisher
  6. Ahn B, Coblentz P, Beharry A, Patel N, Judge A, Moylan J, et al. Diaphragm Abnormalities in Patients with End-Stage Heart Failure: NADPH Oxidase Upregulation and Protein Oxidation. Front Physiol. 2016;7:686 pubmed publisher
  7. Patche J, Girard D, Catan A, Boyer F, Dobi A, Planesse C, et al. Diabetes-induced hepatic oxidative stress: a new pathogenic role for glycated albumin. Free Radic Biol Med. 2017;102:133-148 pubmed publisher
  8. Ballmann C, Denney T, Beyers R, Quindry T, Romero M, Amin R, et al. Lifelong quercetin enrichment and cardioprotection in Mdx/Utrn+/- mice. Am J Physiol Heart Circ Physiol. 2017;312:H128-H140 pubmed publisher
  9. Dubey A, Copeland P. The Selenocysteine-Specific Elongation Factor Contains Unique Sequences That Are Required for Both Nuclear Export and Selenocysteine Incorporation. PLoS ONE. 2016;11:e0165642 pubmed publisher
  10. Cumming K, Ellefsen S, Rønnestad B, Ugelstad I, Raastad T. Acute and long-term effects of blood flow restricted training on heat shock proteins and endogenous antioxidant systems. Scand J Med Sci Sports. 2017;27:1190-1201 pubmed publisher
  11. Mowry A, Kavazis A, Sirman A, Potts W, Hood W. Reproduction Does Not Adversely Affect Liver Mitochondrial Respiratory Function but Results in Lipid Peroxidation and Increased Antioxidants in House Mice. PLoS ONE. 2016;11:e0160883 pubmed publisher
  12. Shanmugam G, Narasimhan M, Sakthivel R, Kumar R R, Davidson C, Palaniappan S, et al. A biphasic effect of TNF-? in regulation of the Keap1/Nrf2 pathway in cardiomyocytes. Redox Biol. 2016;9:77-89 pubmed publisher
  13. Tweedie D, Fukui K, Li Y, Yu Q, Barak S, Tamargo I, et al. Cognitive Impairments Induced by Concussive Mild Traumatic Brain Injury in Mouse Are Ameliorated by Treatment with Phenserine via Multiple Non-Cholinergic and Cholinergic Mechanisms. PLoS ONE. 2016;11:e0156493 pubmed publisher
  14. Li B, Guo W, Hong L, Liu Y, Liu C, Hong S, et al. Role of mechanical strain-activated PI3K/Akt signaling pathway in pelvic organ prolapse. Mol Med Rep. 2016;14:243-53 pubmed publisher
  15. Shimada K, Skouta R, Kaplan A, Yang W, Hayano M, Dixon S, et al. Global survey of cell death mechanisms reveals metabolic regulation of ferroptosis. Nat Chem Biol. 2016;12:497-503 pubmed publisher
  16. Myers C. Enhanced targeting of mitochondrial peroxide defense by the combined use of thiosemicarbazones and inhibitors of thioredoxin reductase. Free Radic Biol Med. 2016;91:81-92 pubmed publisher
  17. Bagulho A, Vilas Boas F, Pena A, Peneda C, Santos F, Jerónimo A, et al. The extracellular matrix modulates H2O2 degradation and redox signaling in endothelial cells. Redox Biol. 2015;6:454-460 pubmed publisher
  18. Musicki B, Zhang Y, Chen H, Brown T, Zirkin B, Burnett A. Mechanism of testosterone deficiency in the transgenic sickle cell mouse. PLoS ONE. 2015;10:e0128694 pubmed publisher
  19. Wang J, Shanmugam A, Markand S, Zorrilla E, Ganapathy V, Smith S. Sigma 1 receptor regulates the oxidative stress response in primary retinal Müller glial cells via NRF2 signaling and system xc(-), the Na(+)-independent glutamate-cystine exchanger. Free Radic Biol Med. 2015;86:25-36 pubmed publisher
  20. Baqader N, Radulovic M, Crawford M, Stoeber K, Godovac Zimmermann J. Nuclear cytoplasmic trafficking of proteins is a major response of human fibroblasts to oxidative stress. J Proteome Res. 2014;13:4398-423 pubmed publisher
  21. Dalmases M, Torres M, Márquez Kisinousky L, Almendros I, Planas A, Embid C, et al. Brain tissue hypoxia and oxidative stress induced by obstructive apneas is different in young and aged rats. Sleep. 2014;37:1249-56 pubmed publisher
  22. Moura C, Lollo P, Morato P, Nisishima L, Carneiro E, Amaya Farfan J. Whey protein hydrolysate enhances HSP90 but does not alter HSP60 and HSP25 in skeletal muscle of rats. PLoS ONE. 2014;9:e83437 pubmed publisher
  23. Schweikl H, Petzel C, Bolay C, Hiller K, Buchalla W, Krifka S. 2-Hydroxyethyl methacrylate-induced apoptosis through the ATM- and p53-dependent intrinsic mitochondrial pathway. Biomaterials. 2014;35:2890-904 pubmed publisher
  24. Yuki K, Yoshida T, Miyake S, Tsubota K, Ozawa Y. Neuroprotective role of superoxide dismutase 1 in retinal ganglion cells and inner nuclear layer cells against N-methyl-d-aspartate-induced cytotoxicity. Exp Eye Res. 2013;115:230-8 pubmed publisher
  25. Wu C, Tang S, Wang P, Lee H, Ko J. Nickel-induced epithelial-mesenchymal transition by reactive oxygen species generation and E-cadherin promoter hypermethylation. J Biol Chem. 2012;287:25292-302 pubmed publisher