This is a Validated Antibody Database (VAD) review about rat Gjd2, based on 21 published articles (read how Labome selects the articles), using Gjd2 antibody in all methods. It is aimed to help Labome visitors find the most suited Gjd2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Gjd2 synonym: Cx36; Gja9; gap junction delta-2 protein; connexin-36; gap junction alpha-9 protein; gap junction membrane channel protein alpha 9

Knockout validation
Invitrogen
rabbit polyclonal
  • immunohistochemistry knockout validation; mouse; 1:500; fig 1
In order to test if disruption of rod-cone coupling prevents secondary cone death and reduces the spread of degeneration, Invitrogen Gjd2 antibody (Invitrogen, 51-6300) was used in immunohistochemistry knockout validation on mouse samples at 1:500 (fig 1). PLoS ONE (2013) ncbi
Invitrogen
rabbit polyclonal
  • immunocytochemistry; mouse; loading ...; fig 1d
In order to explore if the anti-diabetic sulphonylurea glibenclamide protects insulin-producing cells against conditions mimicking those expected at the onset of type 1 diabetes, Invitrogen Gjd2 antibody (Invitrogen, 51-6200) was used in immunocytochemistry on mouse samples (fig 1d). PLoS ONE (2016) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000; fig 4d
In order to assess the contributions of Sig-1Rs and connexin 43 to the induction of central neuropathic pain during spinal cord injury, Invitrogen Gjd2 antibody (Invitrogen, 51-6200) was used in western blot on mouse samples at 1:1000 (fig 4d). Neuropharmacology (2016) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse; 1:500; fig s8
  • western blot; mouse; 1:250; fig 6
In order to propose that electrical coupling between neocortical interneurons regulates chemical synapse development and circuit formation, Invitrogen Gjd2 antibody (Invitrogen, 51-6300) was used in immunohistochemistry on mouse samples at 1:500 (fig s8) and in western blot on mouse samples at 1:250 (fig 6). Nat Commun (2016) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse; 1:500; loading ...; tbl 1
In order to characterize Brn3 null mice, Invitrogen Gjd2 antibody (Invitrogen, 51-6200) was used in immunohistochemistry on mouse samples at 1:500 (tbl 1). J Comp Neurol (2019) ncbi
rabbit polyclonal
  • western blot; mouse; 1:250; fig 2
In order to study differential expression of astrocytic connexins by a prenatal alcohol exposure mouse model, Invitrogen Gjd2 antibody (Invitrogen, 516200) was used in western blot on mouse samples at 1:250 (fig 2). Neurobiol Dis (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; 1:500
In order to investigate the counterpart of connexin36 at gap junctions between rods and cones, Invitrogen Gjd2 antibody (Invitrogen, 51-6300) was used in immunohistochemistry - frozen section on mouse samples at 1:500. J Mol Neurosci (2016) ncbi
rabbit polyclonal
  • western blot; rat; 1:300; fig 9
In order to investigate the role of baclofen on hippocampal CA1 pyramidal cells and its effect on reversing neuronal cell damage under chronic cerebral hyperprofusion, Invitrogen Gjd2 antibody (Invitrogen, 51-6300) was used in western blot on rat samples at 1:300 (fig 9). Sci Rep (2015) ncbi
rabbit polyclonal
  • western blot; mouse; 1:100; fig 5
In order to determine if Cx36 gap junctions contribute to neuronal death via channel-dependent or channel-independent mechanisms, Invitrogen Gjd2 antibody (Invitrogen, 51-6300) was used in western blot on mouse samples at 1:100 (fig 5). PLoS ONE (2015) ncbi
rabbit polyclonal
  • immunohistochemistry - frozen section; rat; 1:400
In order to research light-damaged albino rats and their gap junction proteins, Invitrogen Gjd2 antibody (Life Technologies, 51?C6300) was used in immunohistochemistry - frozen section on rat samples at 1:400. Mol Vis (2014) ncbi
rabbit polyclonal
  • western blot; rat; 1:1000; fig 1
In order to show that suppression of spinal connexin 43 after nerve injury alleviates neuropathic mechanical hypersensitivity, Invitrogen Gjd2 antibody (Life technologies, 51-6200) was used in western blot on rat samples at 1:1000 (fig 1). Neurosci Lett (2014) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse; 1-2 ug/ml
  • immunohistochemistry; rat; 1-2 ug/ml
In order to investigate gap junction-mediated communcation between nitrergic enteric neurons, Invitrogen Gjd2 antibody (Life Technologies Corporation, 51-6300) was used in immunohistochemistry on mouse samples at 1-2 ug/ml and in immunohistochemistry on rat samples at 1-2 ug/ml. FEBS Lett (2014) ncbi
rabbit polyclonal
  • immunohistochemistry; rat
In order to investigate gap junction-mediated communcation on trigeminal motoneurons and at primary afferent terminals on spinal cord neurons in adult mouse and rat, Invitrogen Gjd2 antibody (Life Technologies Corporation, 51-6300) was used in immunohistochemistry on rat samples . Neuroscience (2014) ncbi
rabbit polyclonal
  • immunocytochemistry; mouse
  • immunocytochemistry; rat
In order to characterize connexins associated with motoneurons in rodent spinal cord, sexually dimorphic motor nuclei and trigeminal motor nucleus, Invitrogen Gjd2 antibody (Life Technologies, 51-6300) was used in immunocytochemistry on mouse samples and in immunocytochemistry on rat samples . Eur J Neurosci (2014) ncbi
rabbit polyclonal
  • immunocytochemistry; mouse
In order to identify gap junction-mediated communcation between motoneurons in sexually dimorphic motor nuclei in spinal cord of rat and mouse, Invitrogen Gjd2 antibody (Invitrogen/Zymed Laboratories, 51-6300) was used in immunocytochemistry on mouse samples . Eur J Neurosci (2014) ncbi
rabbit polyclonal
  • immunocytochemistry; human; fig s1
In order to investigate the functional and molecular complexities of electrical synapses in fish, Invitrogen Gjd2 antibody (Life Technologies, 51-6300) was used in immunocytochemistry on human samples (fig s1). Neuron (2013) ncbi
rabbit polyclonal
  • immunohistochemistry knockout validation; mouse; 1:500; fig 1
In order to test if disruption of rod-cone coupling prevents secondary cone death and reduces the spread of degeneration, Invitrogen Gjd2 antibody (Invitrogen, 51-6300) was used in immunohistochemistry knockout validation on mouse samples at 1:500 (fig 1). PLoS ONE (2013) ncbi
rabbit polyclonal
  • western blot; rat
In order to test if circadian electrical oscillations are synchronized by changes in the expression of connexins and alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor subunits, Invitrogen Gjd2 antibody (Invitrogen, 51-6300) was used in western blot on rat samples . Neuroscience (2012) ncbi
rabbit polyclonal
  • western blot; rat
In order to test if circadian electrical oscillations are synchronized by changes in the expression of connexins and alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor subunits, Invitrogen Gjd2 antibody (Invitrogen, 51-6200) was used in western blot on rat samples . Neuroscience (2012) ncbi
rabbit polyclonal
  • immunohistochemistry; rat
In order to report "mixed" (electrical/chemical) synapses on both principal cells and interneurons in adult rat hippocampus, Invitrogen Gjd2 antibody (Invitrogen (Life Technologies), 51-6300) was used in immunohistochemistry on rat samples . Front Neuroanat (2012) ncbi
rabbit polyclonal
  • western blot; mouse; 0.5 ug/ml; fig 1
In order to report that the ischemic increase in neuronal gap junction coupling is regulated by glutamate via group II metabotropic glutamate receptors, Invitrogen Gjd2 antibody (Zymed, 51-6300) was used in western blot on mouse samples at 0.5 ug/ml (fig 1). J Neurosci (2012) ncbi
rabbit polyclonal
  • western blot; mouse; 0.5 ug/ml; fig 6
In order to investigate the mechanism by which group II metabotropic glutamate receptors regulate connexin-36, Invitrogen Gjd2 antibody (Zymed, 51-6300) was used in western blot on mouse samples at 0.5 ug/ml (fig 6). J Neurosci (2011) ncbi
rabbit polyclonal
  • immunocytochemistry; rat; 1:400; fig 3
  • western blot; rat; 1:500; fig 1
In order to discuss the role of Cx36 in neural development, Invitrogen Gjd2 antibody (Zymed, 51-6300) was used in immunocytochemistry on rat samples at 1:400 (fig 3) and in western blot on rat samples at 1:500 (fig 1). PLoS ONE (2011) ncbi
Articles Reviewed
  1. Lamprianou S, Gysemans C, Bou Saab J, Pontes H, Mathieu C, Meda P. Glibenclamide Prevents Diabetes in NOD Mice. PLoS ONE. 2016;11:e0168839 pubmed publisher
  2. Choi S, Roh D, Yoon S, Kwon S, Choi H, Han H, et al. Astrocyte sigma-1 receptors modulate connexin 43 expression leading to the induction of below-level mechanical allodynia in spinal cord injured mice. Neuropharmacology. 2016;111:34-46 pubmed publisher
  3. Yao X, Wang M, He X, He F, Zhang S, Lu W, et al. Electrical coupling regulates layer 1 interneuron microcircuit formation in the neocortex. Nat Commun. 2016;7:12229 pubmed publisher
  4. Ghinia M, Novelli E, Sajgo S, Badea T, Strettoi E. Brn3a and Brn3b knockout mice display unvaried retinal fine structure despite major morphological and numerical alterations of ganglion cells. J Comp Neurol. 2019;527:187-211 pubmed publisher
  5. Ramani M, Mylvaganam S, Krawczyk M, Wang L, Zoidl C, Brien J, et al. Differential expression of astrocytic connexins in a mouse model of prenatal alcohol exposure. Neurobiol Dis. 2016;91:83-93 pubmed publisher
  6. Bolte P, Herrling R, Dorgau B, Schultz K, Feigenspan A, Weiler R, et al. Expression and Localization of Connexins in the Outer Retina of the Mouse. J Mol Neurosci. 2016;58:178-92 pubmed publisher
  7. Liu L, Li C, Lu Y, Zong X, Luo C, Sun J, et al. Baclofen mediates neuroprotection on hippocampal CA1 pyramidal cells through the regulation of autophagy under chronic cerebral hypoperfusion. Sci Rep. 2015;5:14474 pubmed publisher
  8. Fontes J, Ramsey J, Polk J, Koop A, Denisova J, Belousov A. Death of Neurons following Injury Requires Conductive Neuronal Gap Junction Channels but Not a Specific Connexin. PLoS ONE. 2015;10:e0125395 pubmed publisher
  9. Guo C, Tran H, Green C, Danesh Meyer H, Acosta M. Gap junction proteins in the light-damaged albino rat. Mol Vis. 2014;20:670-82 pubmed
  10. Xu Q, Cheong Y, He S, Tiwari V, Liu J, Wang Y, et al. Suppression of spinal connexin 43 expression attenuates mechanical hypersensitivity in rats after an L5 spinal nerve injury. Neurosci Lett. 2014;566:194-199 pubmed publisher
  11. Nagy J, Urena Ramirez V, Ghia J. Functional alterations in gut contractility after connexin36 ablation and evidence for gap junctions forming electrical synapses between nitrergic enteric neurons. FEBS Lett. 2014;588:1480-90 pubmed publisher
  12. Bautista W, McCrea D, Nagy J. Connexin36 identified at morphologically mixed chemical/electrical synapses on trigeminal motoneurons and at primary afferent terminals on spinal cord neurons in adult mouse and rat. Neuroscience. 2014;263:159-80 pubmed publisher
  13. Bautista W, Rash J, Vanderpool K, Yasumura T, Nagy J. Re-evaluation of connexins associated with motoneurons in rodent spinal cord, sexually dimorphic motor nuclei and trigeminal motor nucleus. Eur J Neurosci. 2014;39:757-70 pubmed publisher
  14. Bautista W, Nagy J. Connexin36 in gap junctions forming electrical synapses between motoneurons in sexually dimorphic motor nuclei in spinal cord of rat and mouse. Eur J Neurosci. 2014;39:771-87 pubmed publisher
  15. Rash J, Curti S, Vanderpool K, Kamasawa N, Nannapaneni S, Palacios Prado N, et al. Molecular and functional asymmetry at a vertebrate electrical synapse. Neuron. 2013;79:957-69 pubmed publisher
  16. Kranz K, Paquet Durand F, Weiler R, Janssen Bienhold U, Dedek K. Testing for a gap junction-mediated bystander effect in retinitis pigmentosa: secondary cone death is not altered by deletion of connexin36 from cones. PLoS ONE. 2013;8:e57163 pubmed publisher
  17. Corthell J, Fadool D, Trombley P. Connexin and AMPA receptor expression changes over time in the rat olfactory bulb. Neuroscience. 2012;222:38-48 pubmed publisher
  18. Hamzei Sichani F, Davidson K, Yasumura T, Janssen W, Wearne S, Hof P, et al. Mixed Electrical-Chemical Synapses in Adult Rat Hippocampus are Primarily Glutamatergic and Coupled by Connexin-36. Front Neuroanat. 2012;6:13 pubmed publisher
  19. Wang Y, Song J, Denisova J, Park W, Fontes J, Belousov A. Neuronal gap junction coupling is regulated by glutamate and plays critical role in cell death during neuronal injury. J Neurosci. 2012;32:713-25 pubmed publisher
  20. Park W, Wang Y, Park S, Denisova J, Fontes J, Belousov A. Interplay of chemical neurotransmitters regulates developmental increase in electrical synapses. J Neurosci. 2011;31:5909-20 pubmed publisher
  21. Hartfield E, Rinaldi F, Glover C, Wong L, Caldwell M, Uney J. Connexin 36 expression regulates neuronal differentiation from neural progenitor cells. PLoS ONE. 2011;6:e14746 pubmed publisher