This is a Validated Antibody Database (VAD) review about rat Gja8, based on 6 published articles (read how Labome selects the articles), using Gja8 antibody in all methods. It is aimed to help Labome visitors find the most suited Gja8 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Gja8 synonym: Cx50; gap junction alpha-8 protein; connexin-50; gap junction membrane channel protein alpha 8

Invitrogen
mouse monoclonal (C6)
  • immunocytochemistry; rat; 1:200; fig 1
  • western blot; rat; 1:500; fig 1
In order to investigate modulation of Sox2 expression in spinal-cord-derived ependymal stem/progenitor cells by connexin 50, Invitrogen Gja8 antibody (Life Technologies, 33-4300) was used in immunocytochemistry on rat samples at 1:200 (fig 1) and in western blot on rat samples at 1:500 (fig 1). Cell Tissue Res (2016) ncbi
mouse monoclonal (C6)
  • immunohistochemistry - frozen section; mouse; 1:1000
In order to investigate the counterpart of connexin36 at gap junctions between rods and cones, Invitrogen Gja8 antibody (Invitrogen, 33-4300) was used in immunohistochemistry - frozen section on mouse samples at 1:1000. J Mol Neurosci (2016) ncbi
mouse monoclonal (C6)
  • immunocytochemistry; mouse; 0.7 ug/ml
  • immunohistochemistry; mouse; 0.5 ug/ml
  • western blot; mouse; 0.2 ug/ml
In order to investigate homotypic gap junctions of Connexin50 in axon terminals of mouse horizontal cells, Invitrogen Gja8 antibody (Invitrogen, 33-4300) was used in immunocytochemistry on mouse samples at 0.7 ug/ml, in immunohistochemistry on mouse samples at 0.5 ug/ml and in western blot on mouse samples at 0.2 ug/ml. J Comp Neurol (2015) ncbi
mouse monoclonal (C6)
  • western blot; mouse; fig 2
In order to use knock out mice to determine the role of PKCgamma in the regulation of gap junction coupling in the normal lens, Invitrogen Gja8 antibody (Zymed-Invitrogen, 33-4300) was used in western blot on mouse samples (fig 2). Curr Eye Res (2011) ncbi
mouse monoclonal (C6)
  • immunohistochemistry - paraffin section; human; 1:1000; fig 1
In order to examine connexin expression in chromaffin cells of normal human adrenal glands and benign and malignant pheochromocytomas, Invitrogen Gja8 antibody (ZYMED, 33-4300) was used in immunohistochemistry - paraffin section on human samples at 1:1000 (fig 1). Ann N Y Acad Sci (2006) ncbi
mouse monoclonal (C6)
  • immunohistochemistry - frozen section; rabbit; 5-10 ug/ml
In order to study connexins in mammalian Muller cells, Invitrogen Gja8 antibody (Zymed, 33-4300) was used in immunohistochemistry - frozen section on rabbit samples at 5-10 ug/ml. Vis Neurosci (2006) ncbi
Articles Reviewed
  1. Rodríguez Jiménez F, Alastrue A, Stojkovic M, Erceg S, Moreno Manzano V. Connexin 50 modulates Sox2 expression in spinal-cord-derived ependymal stem/progenitor cells. Cell Tissue Res. 2016;365:295-307 pubmed publisher
  2. Bolte P, Herrling R, Dorgau B, Schultz K, Feigenspan A, Weiler R, et al. Expression and Localization of Connexins in the Outer Retina of the Mouse. J Mol Neurosci. 2016;58:178-92 pubmed publisher
  3. Dorgau B, Herrling R, Schultz K, Greb H, Segelken J, Ströh S, et al. Connexin50 couples axon terminals of mouse horizontal cells by homotypic gap junctions. J Comp Neurol. 2015;523:2062-81 pubmed publisher
  4. Das S, Wang H, Molina S, Martinez Wittinghan F, Jena S, Bossmann L, et al. PKC?, role in lens differentiation and gap junction coupling. Curr Eye Res. 2011;36:620-31 pubmed publisher
  5. Willenberg H, Schott M, Saeger W, Tries A, Scherbaum W, Bornstein S. Expression of connexins in chromaffin cells of normal human adrenals and in benign and malignant pheochromocytomas. Ann N Y Acad Sci. 2006;1073:578-83 pubmed
  6. Zahs K, Ceelen P. Gap junctional coupling and connexin immunoreactivity in rabbit retinal glia. Vis Neurosci. 2006;23:1-10 pubmed