This is a Validated Antibody Database (VAD) review about rat Flot2, based on 19 published articles (read how Labome selects the articles), using Flot2 antibody in all methods. It is aimed to help Labome visitors find the most suited Flot2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Flot2 synonym: flotillin-2; REG-1; reggie-1; reggie1-1

Santa Cruz Biotechnology
mouse monoclonal (B-6)
  • western blot; human; loading ...; fig 1b
Santa Cruz Biotechnology Flot2 antibody (Santa Cruz Biotechnology, sc-28320) was used in western blot on human samples (fig 1b). Biol Open (2019) ncbi
mouse monoclonal (B-6)
  • western blot; human; loading ...; fig 3
Santa Cruz Biotechnology Flot2 antibody (Santa Cruz, sc-28320) was used in western blot on human samples (fig 3). J Proteome Res (2017) ncbi
mouse monoclonal (B-6)
  • western blot; human; loading ...; fig 4c
In order to study NPC1L1 gene, proteome and ezetimibe, Santa Cruz Biotechnology Flot2 antibody (SantaCruz, SC-28320) was used in western blot on human samples (fig 4c). Atherosclerosis (2016) ncbi
mouse monoclonal (B-6)
  • immunocytochemistry; mouse; 1:50; fig 4
Santa Cruz Biotechnology Flot2 antibody (Santa Cruz, sc-28320) was used in immunocytochemistry on mouse samples at 1:50 (fig 4). Pflugers Arch (2015) ncbi
Abcam
rabbit monoclonal (EPR14128(B))
  • western blot; human; loading ...; fig 5d, s4f
Abcam Flot2 antibody (Abcam, ab181988) was used in western blot on human samples (fig 5d, s4f). Cell (2019) ncbi
MilliporeSigma
rabbit polyclonal
  • western blot; human
MilliporeSigma Flot2 antibody (Sigma-Aldrich, F1805) was used in western blot on human samples . FEBS J (2015) ncbi
Cell Signaling Technology
rabbit monoclonal (C42A3)
  • western blot; human; loading ...; fig 1b
  • western blot; mouse; loading ...; fig 1b
Cell Signaling Technology Flot2 antibody (Cell Signaling, 3436) was used in western blot on human samples (fig 1b) and in western blot on mouse samples (fig 1b). Dev Cell (2017) ncbi
rabbit monoclonal (C42A3)
  • western blot; human; loading ...; fig 1b
In order to dose MDAMB231 breast cancer cells with paclitaxel, causing them to generate exosomes highly enriched with Survivin, Cell Signaling Technology Flot2 antibody (Cell Signaling, 3436S) was used in western blot on human samples (fig 1b). Cancers (Basel) (2016) ncbi
rabbit monoclonal (C42A3)
  • western blot; mouse; 1:1000; loading ...; fig 6a
  • western blot; human; 1:1000; loading ...; fig 7c
In order to report that oncogenic transformation results in microvesicles, Cell Signaling Technology Flot2 antibody (Cell Signaling, 3436S) was used in western blot on mouse samples at 1:1000 (fig 6a) and in western blot on human samples at 1:1000 (fig 7c). J Biol Chem (2016) ncbi
rabbit monoclonal (C42A3)
  • western blot; mouse; 1:2000; loading ...; fig 4b
Cell Signaling Technology Flot2 antibody (Cell Signaling Technology, 3436) was used in western blot on mouse samples at 1:2000 (fig 4b). Nat Commun (2016) ncbi
rabbit monoclonal (C42A3)
  • immunoprecipitation; human
  • western blot; human
In order to investigate flotillin-1-mediated regulation of insulin-like growth factor-1 signaling, Cell Signaling Technology Flot2 antibody (Cell Signaling Technology, 3436) was used in immunoprecipitation on human samples and in western blot on human samples . J Cell Sci (2015) ncbi
rabbit monoclonal (C42A3)
  • western blot; mouse
In order to study the effects of ATRAP in the renal distal tubules in response to high salt treatment using wild type and ATRAP transgenic mice, Cell Signaling Technology Flot2 antibody (Cell Signaling Technology, 3436) was used in western blot on mouse samples . J Am Heart Assoc (2015) ncbi
BD Biosciences
mouse monoclonal (29/Flotillin-2)
  • western blot; mouse; fig 2a
In order to describe extracellular vesicle subpopulations secreted by fat cells, BD Biosciences Flot2 antibody (BD Biosciences, 610383) was used in western blot on mouse samples (fig 2a). J Extracell Vesicles (2017) ncbi
mouse monoclonal (29/Flotillin-2)
  • immunocytochemistry; human; 1:500; loading ...; fig S1b
  • western blot; human; 1:5000; loading ...; fig 1e, 5a
BD Biosciences Flot2 antibody (BD Biosciences, 610383) was used in immunocytochemistry on human samples at 1:500 (fig S1b) and in western blot on human samples at 1:5000 (fig 1e, 5a). J Cell Sci (2017) ncbi
mouse monoclonal (29/Flotillin-2)
  • western blot; human; fig 3
In order to characterize induction of apoptosis in SH-SY5Y human neuroblastoma cells through up regulation of flotillin-2 in the lipid raft and the activation of BDNF/Trkb signaling due to cholesterol overload, BD Biosciences Flot2 antibody (BD Biosciences, 610383) was used in western blot on human samples (fig 3). Neuroscience (2016) ncbi
mouse monoclonal (29/Flotillin-2)
  • western blot; human; fig s3
BD Biosciences Flot2 antibody (BD, 610383) was used in western blot on human samples (fig s3). J Mol Cell Biol (2015) ncbi
mouse monoclonal (29/Flotillin-2)
  • western blot; human; fig 3
BD Biosciences Flot2 antibody (BD Biosciences, 610384) was used in western blot on human samples (fig 3). Basic Res Cardiol (2014) ncbi
mouse monoclonal (29/Flotillin-2)
  • western blot; mouse
In order to study the mechanisms underlying the increased hepatic lipid raft alkaline phosphatase activity observed in a dystrophic mouse model, BD Biosciences Flot2 antibody (BD Biosciences, 610383) was used in western blot on mouse samples . Chem Biol Interact (2014) ncbi
mouse monoclonal (29/Flotillin-2)
  • western blot; human
BD Biosciences Flot2 antibody (BD Transduction Laboratories, clone 29) was used in western blot on human samples . BMC Cancer (2014) ncbi
Articles Reviewed
  1. Xiong X, Lee C, Li W, Yu J, Zhu L, Kim Y, et al. Acid Sphingomyelinase regulates the localization and trafficking of palmitoylated proteins. Biol Open. 2019;: pubmed publisher
  2. Jeppesen D, Fenix A, Franklin J, Higginbotham J, Zhang Q, Zimmerman L, et al. Reassessment of Exosome Composition. Cell. 2019;177:428-445.e18 pubmed publisher
  3. Guo H, Chitiprolu M, Roncevic L, Javalet C, Hemming F, Trung M, et al. Atg5 Disassociates the V1V0-ATPase to Promote Exosome Production and Tumor Metastasis Independent of Canonical Macroautophagy. Dev Cell. 2017;43:716-730.e7 pubmed publisher
  4. Durcin M, Fleury A, Taillebois E, Hilairet G, Krupova Z, Henry C, et al. Characterisation of adipocyte-derived extracellular vesicle subtypes identifies distinct protein and lipid signatures for large and small extracellular vesicles. J Extracell Vesicles. 2017;6:1305677 pubmed publisher
  5. Ludwig A, Nguyen T, Leong D, Ravi L, Tan B, Sandin S, et al. Caveolae provide a specialized membrane environment for respiratory syncytial virus assembly. J Cell Sci. 2017;130:1037-1050 pubmed publisher
  6. Staubach S, Müller S, Pekmez M, Hanisch F. Classical Galactosemia: Insight into Molecular Pathomechanisms by Differential Membrane Proteomics of Fibroblasts under Galactose Stress. J Proteome Res. 2017;16:516-527 pubmed publisher
  7. Kreger B, Johansen E, Cerione R, Antonyak M. The Enrichment of Survivin in Exosomes from Breast Cancer Cells Treated with Paclitaxel Promotes Cell Survival and Chemoresistance. Cancers (Basel). 2016;8: pubmed
  8. Kreger B, Dougherty A, Greene K, Cerione R, Antonyak M. Microvesicle Cargo and Function Changes upon Induction of Cellular Transformation. J Biol Chem. 2016;291:19774-85 pubmed publisher
  9. Desrochers L, Bordeleau F, Reinhart King C, Cerione R, Antonyak M. Microvesicles provide a mechanism for intercellular communication by embryonic stem cells during embryo implantation. Nat Commun. 2016;7:11958 pubmed publisher
  10. Huang Y, Lin C, Liao H, Liu C, Chen Y, Chiu W, et al. Cholesterol overload induces apoptosis in SH-SY5Y human neuroblastoma cells through the up regulation of flotillin-2 in the lipid raft and the activation of BDNF/Trkb signaling. Neuroscience. 2016;328:201-9 pubmed publisher
  11. Schweitzer M, Makhoul S, Paliouras M, Beitel L, Gottlieb B, Trifiro M, et al. Characterization of the NPC1L1 gene and proteome from an exceptional responder to ezetimibe. Atherosclerosis. 2016;246:78-86 pubmed publisher
  12. Eckhard A, Dos Santos A, Liu W, Bassiouni M, Arnold H, Gleiser C, et al. Regulation of the perilymphatic-endolymphatic water shunt in the cochlea by membrane translocation of aquaporin-5. Pflugers Arch. 2015;467:2571-88 pubmed publisher
  13. Jang D, Kwon H, Jeong K, Lee J, Pak Y. Essential role of flotillin-1 palmitoylation in the intracellular localization and signaling function of IGF-1 receptor. J Cell Sci. 2015;128:2179-90 pubmed publisher
  14. Inoue M, Hur J, Kihara T, Teranishi Y, Yamamoto N, Ishikawa T, et al. Human brain proteins showing neuron-specific interactions with γ-secretase. FEBS J. 2015;282:2587-99 pubmed publisher
  15. Wakui H, Uneda K, Tamura K, Ohsawa M, Azushima K, Kobayashi R, et al. Renal tubule angiotensin II type 1 receptor-associated protein promotes natriuresis and inhibits salt-sensitive blood pressure elevation. J Am Heart Assoc. 2015;4:e001594 pubmed publisher
  16. Menck K, Scharf C, Bleckmann A, Dyck L, Rost U, Wenzel D, et al. Tumor-derived microvesicles mediate human breast cancer invasion through differentially glycosylated EMMPRIN. J Mol Cell Biol. 2015;7:143-53 pubmed publisher
  17. Fork C, Hitzel J, Nichols B, Tikkanen R, Brandes R. Flotillin-1 facilitates toll-like receptor 3 signaling in human endothelial cells. Basic Res Cardiol. 2014;109:439 pubmed publisher
  18. Montenegro M, Moral Naranjo M, Campoy F, Muñoz Delgado E, Vidal C. The lipid raft-bound alkaline phosphatase activity increases and the level of transcripts remains unaffected in liver of merosin-deficient LAMA2dy mouse. Chem Biol Interact. 2014;216:1-8 pubmed publisher
  19. Arkhipova K, Sheyderman A, Laktionov K, Mochalnikova V, Zborovskaya I. Simultaneous expression of flotillin-1, flotillin-2, stomatin and caveolin-1 in non-small cell lung cancer and soft tissue sarcomas. BMC Cancer. 2014;14:100 pubmed publisher