This is a Validated Antibody Database (VAD) review about rat Fhl2, based on 4 published articles (read how Labome selects the articles), using Fhl2 antibody in all methods. It is aimed to help Labome visitors find the most suited Fhl2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Fhl2 synonym: SLIM 3; four and a half LIM domains protein 2; FHL-2; LIM domain protein DRAL; SLIM-3; skeletal muscle LIM-protein 3

Proteintech Group
rabbit polyclonal
  • flow cytometry; human; 1:1000; loading ...; fig 10
  • western blot; human; 1:1000; loading ...; fig 10c
Proteintech Group Fhl2 antibody (Proteintech, 21619-1-AP) was used in flow cytometry on human samples at 1:1000 (fig 10) and in western blot on human samples at 1:1000 (fig 10c). Nat Commun (2017) ncbi
Invitrogen
mouse monoclonal (F4B2-B11)
  • immunocytochemistry; human; loading ...; fig 1i
  • western blot; human; loading ...; fig 1f
  • immunocytochemistry; mouse; loading ...; fig 1d
  • western blot; mouse; loading ...; fig 1c
In order to examine the localization and function of FHL2 in natural killer cells, Invitrogen Fhl2 antibody (Thermo Fisher Scientific, F4B2-B11) was used in immunocytochemistry on human samples (fig 1i), in western blot on human samples (fig 1f), in immunocytochemistry on mouse samples (fig 1d) and in western blot on mouse samples (fig 1c). Front Immunol (2017) ncbi
Santa Cruz Biotechnology
mouse monoclonal (F4B2-B11)
  • immunoprecipitation; rat; loading ...; fig 4b
  • immunocytochemistry; rat; loading ...; fig 6a
  • western blot; rat; loading ...; fig 5a
In order to report that mammalian actin-binding protein-1 and FHL2 are binding partners that regulate Rho GTPase signaling and breast cancer cell invasion, Santa Cruz Biotechnology Fhl2 antibody (Santa Cruz, F4B2-B11) was used in immunoprecipitation on rat samples (fig 4b), in immunocytochemistry on rat samples (fig 6a) and in western blot on rat samples (fig 5a). J Biol Chem (2016) ncbi
Abcam
rabbit polyclonal
  • immunohistochemistry; mouse
Abcam Fhl2 antibody (Abcam, ab66399) was used in immunohistochemistry on mouse samples . PLoS ONE (2013) ncbi
Articles Reviewed
  1. Baranek T, Morello E, Valayer A, Aimar R, Bréa D, Henry C, et al. FHL2 Regulates Natural Killer Cell Development and Activation during Streptococcus pneumoniae Infection. Front Immunol. 2017;8:123 pubmed publisher
  2. Xu X, Fan Z, Liang C, Li L, Wang L, Liang Y, et al. A signature motif in LIM proteins mediates binding to checkpoint proteins and increases tumour radiosensitivity. Nat Commun. 2017;8:14059 pubmed publisher
  3. Boateng L, Bennin D, de Oliveira S, Huttenlocher A. Mammalian Actin-binding Protein-1/Hip-55 Interacts with FHL2 and Negatively Regulates Cell Invasion. J Biol Chem. 2016;291:13987-98 pubmed publisher
  4. Alnajar A, Nordhoff C, Schied T, Chiquet Ehrismann R, Loser K, Vogl T, et al. The LIM-only protein FHL2 attenuates lung inflammation during bleomycin-induced fibrosis. PLoS ONE. 2013;8:e81356 pubmed publisher