This is a Validated Antibody Database (VAD) review about rat Fasn, based on 24 published articles (read how Labome selects the articles), using Fasn antibody in all methods. It is aimed to help Labome visitors find the most suited Fasn antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Knockout validation
Abcam
domestic rabbit monoclonal (EPR7466)
  • western blot knockout validation; human; 1:2000; loading ...
Abcam Fasn antibody (Abcam, ab128870) was used in western blot knockout validation on human samples at 1:2000. Nat Metab (2020) ncbi
Santa Cruz Biotechnology
mouse monoclonal (A-5)
  • western blot; mouse; 1:1000; loading ...; fig 3b
Santa Cruz Biotechnology Fasn antibody (Santa, sc-55580) was used in western blot on mouse samples at 1:1000 (fig 3b). Nat Commun (2020) ncbi
mouse monoclonal (G-11)
  • immunohistochemistry; mouse; 1:100; loading ...; fig e3c
Santa Cruz Biotechnology Fasn antibody (Santa Cruz, sc48357) was used in immunohistochemistry on mouse samples at 1:100 (fig e3c). Nature (2018) ncbi
mouse monoclonal (A-5)
  • western blot; mouse; loading ...; fig s9a
  • western blot; human; loading ...; fig s7
Santa Cruz Biotechnology Fasn antibody (Santa Cruz, sc-55580) was used in western blot on mouse samples (fig s9a) and in western blot on human samples (fig s7). Sci Signal (2017) ncbi
mouse monoclonal (A-5)
  • western blot; mouse; loading ...; fig 5A
Santa Cruz Biotechnology Fasn antibody (Santa Cruz, sc-55580) was used in western blot on mouse samples (fig 5A). Int J Mol Med (2017) ncbi
mouse monoclonal (A-5)
  • western blot; rat; loading ...; fig 9a
Santa Cruz Biotechnology Fasn antibody (Santa Cruz, sc-55580) was used in western blot on rat samples (fig 9a). Redox Biol (2016) ncbi
mouse monoclonal (A-5)
  • western blot; human; fig 5
Santa Cruz Biotechnology Fasn antibody (Santa Cruz, 55580) was used in western blot on human samples (fig 5). BMC Complement Altern Med (2016) ncbi
mouse monoclonal (A-5)
  • western blot; human; fig 2
In order to elucidate the mechanism of intracellular activation of EGFR by fatty acid synthase dependent palmitoylation, Santa Cruz Biotechnology Fasn antibody (Santa Cruz Biotechnology, sc-55580) was used in western blot on human samples (fig 2). Oncotarget (2015) ncbi
mouse monoclonal (A-5)
  • western blot; rat
Santa Cruz Biotechnology Fasn antibody (Santa Cruz, sc-55580) was used in western blot on rat samples . Cell Physiol Biochem (2015) ncbi
mouse monoclonal (A-5)
  • western blot; mouse; fig 7
  • western blot; human; fig 7
Santa Cruz Biotechnology Fasn antibody (Santa Cruz, sc-55580) was used in western blot on mouse samples (fig 7) and in western blot on human samples (fig 7). Oncogene (2016) ncbi
mouse monoclonal (A-5)
  • western blot; human; 300 ng/ml; fig s6c
Santa Cruz Biotechnology Fasn antibody (Santa cruz, sc-55580) was used in western blot on human samples at 300 ng/ml (fig s6c). Mol Cancer (2015) ncbi
mouse monoclonal (A-5)
  • western blot; human; fig 5b
Santa Cruz Biotechnology Fasn antibody (santa cruz, sc-55580) was used in western blot on human samples (fig 5b). Int J Mol Med (2015) ncbi
mouse monoclonal (A-5)
  • immunohistochemistry - paraffin section; human; fig 4
  • immunoprecipitation; human; fig 5
  • immunocytochemistry; human; fig 4
  • western blot; human; fig 4
In order to analyze EGF induced mitochondrial fusion of cancer cells created by de novo synthesized palmitate and mitochondrial EGFR, Santa Cruz Biotechnology Fasn antibody (Santa Cruz, sc-55580) was used in immunohistochemistry - paraffin section on human samples (fig 4), in immunoprecipitation on human samples (fig 5), in immunocytochemistry on human samples (fig 4) and in western blot on human samples (fig 4). Cell Cycle (2014) ncbi
mouse monoclonal (G-11)
  • immunohistochemistry - paraffin section; mouse; 1:100; fig 1
Santa Cruz Biotechnology Fasn antibody (Santa Cruz, sc-48357) was used in immunohistochemistry - paraffin section on mouse samples at 1:100 (fig 1). Stem Cell Reports (2014) ncbi
mouse monoclonal (G-11)
  • immunohistochemistry - frozen section; mouse
In order to examine the role of keratin 76 in tight junction function and maintenance of the skin barrier, Santa Cruz Biotechnology Fasn antibody (Santa Cruz Biotechnology, sc-48357) was used in immunohistochemistry - frozen section on mouse samples . PLoS Genet (2014) ncbi
Abcam
domestic rabbit monoclonal (EPR7466)
  • western blot knockout validation; human; 1:2000; loading ...
Abcam Fasn antibody (Abcam, ab128870) was used in western blot knockout validation on human samples at 1:2000. Nat Metab (2020) ncbi
domestic rabbit polyclonal
  • western blot; bovine; loading ...; fig 3a
Abcam Fasn antibody (Abcam, ab22759) was used in western blot on bovine samples (fig 3a). Biomed Res Int (2019) ncbi
domestic rabbit monoclonal (EPR7466)
  • western blot; mouse; loading ...; fig 1b
Abcam Fasn antibody (Abcam, ab128870) was used in western blot on mouse samples (fig 1b). EMBO J (2019) ncbi
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig 1f
In order to investigate the contribution of liver receptor homolog 1 SUMOylation to nonalcoholic fatty liver disease development, Abcam Fasn antibody (Abcam, ab22759) was used in western blot on mouse samples (fig 1f). J Clin Invest (2017) ncbi
domestic rabbit monoclonal (EPR7466)
  • western blot; mouse; loading ...; fig 3g
Abcam Fasn antibody (Abcam, ab128870) was used in western blot on mouse samples (fig 3g). Cell Death Dis (2016) ncbi
domestic rabbit polyclonal
  • proximity ligation assay; human; loading ...; fig 1b
  • immunoprecipitation; human; loading ...; fig 1a
  • immunocytochemistry; human; loading ...; fig 1d
  • western blot; human; 1 ug/ml; loading ...; fig 1a
  • proximity ligation assay; mouse; loading ...; fig 1h
  • immunocytochemistry; mouse; loading ...; fig 1f
  • western blot; mouse; 1 ug/ml; loading ...; fig s4c
Abcam Fasn antibody (Abcam, ab22759) was used in proximity ligation assay on human samples (fig 1b), in immunoprecipitation on human samples (fig 1a), in immunocytochemistry on human samples (fig 1d), in western blot on human samples at 1 ug/ml (fig 1a), in proximity ligation assay on mouse samples (fig 1h), in immunocytochemistry on mouse samples (fig 1f) and in western blot on mouse samples at 1 ug/ml (fig s4c). Nat Immunol (2016) ncbi
domestic rabbit monoclonal (EPR7466)
  • immunohistochemistry - paraffin section; human; 1:200; fig 1
In order to analyze metastatic breast cancer and the expression of lipid metabolism-related proteins, Abcam Fasn antibody (Abcam, ab128870) was used in immunohistochemistry - paraffin section on human samples at 1:200 (fig 1). PLoS ONE (2015) ncbi
domestic rabbit monoclonal (EPR7466)
  • immunohistochemistry - paraffin section; human; 1:200; fig 2
  • western blot; human; fig 1
In order to characterize different breast cancer subtypes and differential expression of lipid metabolism-related proteins, Abcam Fasn antibody (Abcam, ab128870) was used in immunohistochemistry - paraffin section on human samples at 1:200 (fig 2) and in western blot on human samples (fig 1). PLoS ONE (2015) ncbi
domestic rabbit monoclonal (EPR7466)
  • western blot; mouse
Abcam Fasn antibody (Abcam, ab128870) was used in western blot on mouse samples . J Lipid Res (2013) ncbi
Novus Biologicals
domestic rabbit polyclonal
  • immunocytochemistry; human; 1:100; fig 3
  • western blot; human; 1:1000; fig 3
Novus Biologicals Fasn antibody (Novus, NB400-114) was used in immunocytochemistry on human samples at 1:100 (fig 3) and in western blot on human samples at 1:1000 (fig 3). Nat Commun (2016) ncbi
Articles Reviewed
  1. Aregger M, Lawson K, Billmann M, Costanzo M, Tong A, Chan K, et al. Systematic mapping of genetic interactions for de novo fatty acid synthesis identifies C12orf49 as a regulator of lipid metabolism. Nat Metab. 2020;2:499-513 pubmed publisher
  2. Viscarra J, Wang Y, Nguyen H, Choi Y, Sul H. Histone demethylase JMJD1C is phosphorylated by mTOR to activate de novo lipogenesis. Nat Commun. 2020;11:796 pubmed publisher
  3. Guo Z, Zhao K, Feng X, Yan D, Yao R, Chen Y, et al. mTORC2 Regulates Lipogenic Gene Expression through PPARγ to Control Lipid Synthesis in Bovine Mammary Epithelial Cells. Biomed Res Int. 2019;2019:5196028 pubmed publisher
  4. Su L, Zhou L, Chen F, Wang H, Qian H, Sheng Y, et al. Cideb controls sterol-regulated ER export of SREBP/SCAP by promoting cargo loading at ER exit sites. EMBO J. 2019;38: pubmed publisher
  5. Liakath Ali K, Mills E, Sequeira I, Lichtenberger B, Pisco A, Sipilä K, et al. An evolutionarily conserved ribosome-rescue pathway maintains epidermal homeostasis. Nature. 2018;556:376-380 pubmed publisher
  6. Viscarra J, Wang Y, Hong I, Sul H. Transcriptional activation of lipogenesis by insulin requires phosphorylation of MED17 by CK2. Sci Signal. 2017;10: pubmed publisher
  7. Kim I, Nam T. Enzyme-treated Ecklonia cava extract inhibits adipogenesis through the downregulation of C/EBP? in 3T3-L1 adipocytes. Int J Mol Med. 2017;39:636-644 pubmed publisher
  8. Stein S, Lemos V, Xu P, Demagny H, Wang X, Ryu D, et al. Impaired SUMOylation of nuclear receptor LRH-1 promotes nonalcoholic fatty liver disease. J Clin Invest. 2017;127:583-592 pubmed publisher
  9. Liu Z, Gan L, Wu T, Feng F, Luo D, Gu H, et al. Adiponectin reduces ER stress-induced apoptosis through PPARα transcriptional regulation of ATF2 in mouse adipose. Cell Death Dis. 2016;7:e2487 pubmed publisher
  10. Serviddio G, Bellanti F, Villani R, Tamborra R, Zerbinati C, Blonda M, et al. Effects of dietary fatty acids and cholesterol excess on liver injury: A lipidomic approach. Redox Biol. 2016;9:296-305 pubmed publisher
  11. Cader M, Boroviak K, Zhang Q, Assadi G, Kempster S, Sewell G, et al. C13orf31 (FAMIN) is a central regulator of immunometabolic function. Nat Immunol. 2016;17:1046-56 pubmed publisher
  12. Jeong H, Cho Y, Kim K, Kim Y, Kim K, Na Y, et al. Anti-lipoapoptotic effects of Alisma orientalis extract on non-esterified fatty acid-induced HepG2 cells. BMC Complement Altern Med. 2016;16:239 pubmed publisher
  13. Karlas A, Berrè S, Couderc T, Varjak M, Braun P, Meyer M, et al. A human genome-wide loss-of-function screen identifies effective chikungunya antiviral drugs. Nat Commun. 2016;7:11320 pubmed publisher
  14. Bollu L, Katreddy R, Blessing A, Pham N, Zheng B, Wu X, et al. Intracellular activation of EGFR by fatty acid synthase dependent palmitoylation. Oncotarget. 2015;6:34992-5003 pubmed publisher
  15. Jung Y, Kim H, Koo J. Expression of Lipid Metabolism-Related Proteins in Metastatic Breast Cancer. PLoS ONE. 2015;10:e0137204 pubmed publisher
  16. Dettlaff Pokora A, Sledzinski T, Swierczynski J. Up-Regulation Mttp and Apob Gene Expression in Rat Liver is Related to Post-Lipectomy Hypertriglyceridemia. Cell Physiol Biochem. 2015;36:1767-77 pubmed
  17. Li S, Oh Y, Yue P, Khuri F, Sun S. Inhibition of mTOR complex 2 induces GSK3/FBXW7-dependent degradation of sterol regulatory element-binding protein 1 (SREBP1) and suppresses lipogenesis in cancer cells. Oncogene. 2016;35:642-50 pubmed publisher
  18. Koizume S, Ito S, Nakamura Y, Yoshihara M, Furuya M, Yamada R, et al. Lipid starvation and hypoxia synergistically activate ICAM1 and multiple genes in an Sp1-dependent manner to promote the growth of ovarian cancer. Mol Cancer. 2015;14:77 pubmed publisher
  19. Kim S, Lee Y, Koo J. Differential expression of lipid metabolism-related proteins in different breast cancer subtypes. PLoS ONE. 2015;10:e0119473 pubmed publisher
  20. Kang O, Kim S, Mun S, Seo Y, Hwang H, Lee Y, et al. Puerarin ameliorates hepatic steatosis by activating the PPARα and AMPK signaling pathways in hepatocytes. Int J Mol Med. 2015;35:803-9 pubmed publisher
  21. Bollu L, Ren J, Blessing A, Katreddy R, Gao G, Xu L, et al. Involvement of de novo synthesized palmitate and mitochondrial EGFR in EGF induced mitochondrial fusion of cancer cells. Cell Cycle. 2014;13:2415-30 pubmed publisher
  22. Kretzschmar K, Cottle D, Donati G, Chiang M, Quist S, Gollnick H, et al. BLIMP1 is required for postnatal epidermal homeostasis but does not define a sebaceous gland progenitor under steady-state conditions. Stem Cell Reports. 2014;3:620-33 pubmed publisher
  23. DiTommaso T, Cottle D, Pearson H, Schlüter H, Kaur P, Humbert P, et al. Keratin 76 is required for tight junction function and maintenance of the skin barrier. PLoS Genet. 2014;10:e1004706 pubmed publisher
  24. De Sousa Coelho A, Relat J, Hondares E, Pérez Martí A, Ribas F, Villarroya F, et al. FGF21 mediates the lipid metabolism response to amino acid starvation. J Lipid Res. 2013;54:1786-97 pubmed publisher