This is a Validated Antibody Database (VAD) review about rat Drd1, based on 16 published articles (read how Labome selects the articles), using Drd1 antibody in all methods. It is aimed to help Labome visitors find the most suited Drd1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Drd1 synonym: D1a; Drd-1; Drd1a

Knockout validation
MilliporeSigma
rat monoclonal (1-1-F11 s.E6)
  • immunohistochemistry - frozen section; human; 1:500; loading ...; fig 2d
  • western blot; human; 1:1000; loading ...; fig 1a
  • immunohistochemistry - frozen section; rat; 1:500; loading ...; fig 2c
  • western blot; rat; 1:1000; loading ...; fig 1a
  • immunohistochemistry knockout validation; mouse; 1:500; loading ...; fig 2a
  • western blot knockout validation; mouse; 1:1000; loading ...; fig 1a
  • immunohistochemistry - frozen section; mouse; 1:500; loading ...; fig 2b
MilliporeSigma Drd1 antibody (Sigma-Aldrich, D2944) was used in immunohistochemistry - frozen section on human samples at 1:500 (fig 2d), in western blot on human samples at 1:1000 (fig 1a), in immunohistochemistry - frozen section on rat samples at 1:500 (fig 2c), in western blot on rat samples at 1:1000 (fig 1a), in immunohistochemistry knockout validation on mouse samples at 1:500 (fig 2a), in western blot knockout validation on mouse samples at 1:1000 (fig 1a) and in immunohistochemistry - frozen section on mouse samples at 1:500 (fig 2b). Amino Acids (2017) ncbi
MilliporeSigma
rat monoclonal (1-1-F11 s.E6)
  • proximity ligation assay; human; fig 6
  • immunohistochemistry knockout validation; mouse; 1:200-1:500; fig 5
  • proximity ligation assay; mouse; fig 6
MilliporeSigma Drd1 antibody (Sigma-Aldrich, D2944) was used in proximity ligation assay on human samples (fig 6), in immunohistochemistry knockout validation on mouse samples at 1:200-1:500 (fig 5) and in proximity ligation assay on mouse samples (fig 6). Mol Psychiatry (2015) ncbi
Abcam
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 2d
Abcam Drd1 antibody (Abcam, ab20066) was used in western blot on mouse samples at 1:1000 (fig 2d). Sci Adv (2020) ncbi
domestic rabbit polyclonal
  • western blot; rat; loading ...; fig 3a
Abcam Drd1 antibody (Abcam, ab20066) was used in western blot on rat samples (fig 3a). BMC Neurosci (2016) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; mouse; 1:4500; fig 3
Abcam Drd1 antibody (Abcam, ab20066) was used in immunohistochemistry on mouse samples at 1:4500 (fig 3). Int J Mol Sci (2016) ncbi
domestic rabbit polyclonal
  • western blot; mouse; fig 7
In order to analyze selective partitioning into complexes and supercomplexes during synapse maturation by NMDA receptors, Abcam Drd1 antibody (Abcam, ab20066) was used in western blot on mouse samples (fig 7). Nat Commun (2016) ncbi
domestic rabbit monoclonal (EP1560Y)
  • western blot; rat; 1:5000; fig 3
Abcam Drd1 antibody (Abcam, ab81296) was used in western blot on rat samples at 1:5000 (fig 3). Front Behav Neurosci (2016) ncbi
domestic rabbit polyclonal
  • western blot; rat; 1:500; loading ...; fig 2
Abcam Drd1 antibody (Abcam, ab-20066) was used in western blot on rat samples at 1:500 (fig 2). Dev Neurobiol (2016) ncbi
domestic rabbit monoclonal (EP1560Y)
  • western blot; human
Abcam Drd1 antibody (Abcam, ab81296) was used in western blot on human samples . Cell Signal (2015) ncbi
domestic rabbit monoclonal (EP1560Y)
  • western blot; mouse; 1:1000
Abcam Drd1 antibody (Abcam, ab81296) was used in western blot on mouse samples at 1:1000. Front Neural Circuits (2014) ncbi
Novus Biologicals
mouse monoclonal (SG2-D1a)
  • western blot; mouse; loading ...
Novus Biologicals Drd1 antibody (Novus Biologicals, NB110-60,017) was used in western blot on mouse samples . Sci Rep (2021) ncbi
mouse monoclonal (SG2-D1a)
  • immunohistochemistry - frozen section; rat; 1:400; loading ...; fig 1e
  • western blot; rat; 1:400; loading ...; fig 4c
In order to test if D1-like receptors regulate duodenal permeability, Novus Biologicals Drd1 antibody (Novus, NB110-60017) was used in immunohistochemistry - frozen section on rat samples at 1:400 (fig 1e) and in western blot on rat samples at 1:400 (fig 4c). Acta Physiol (Oxf) (2017) ncbi
Santa Cruz Biotechnology
mouse monoclonal (SG2-D1a)
  • western blot; mouse; 1:200; fig 4
Santa Cruz Biotechnology Drd1 antibody (Santa Cruz, sc-33660) was used in western blot on mouse samples at 1:200 (fig 4). PLoS ONE (2010) ncbi
MilliporeSigma
rat monoclonal (1-1-F11 s.E6)
  • immunohistochemistry - frozen section; mouse; 1:500; loading ...; fig 6a
MilliporeSigma Drd1 antibody (Sigma-Aldrich, D2944) was used in immunohistochemistry - frozen section on mouse samples at 1:500 (fig 6a). Nat Commun (2022) ncbi
rat monoclonal (1-1-F11 s.E6)
  • immunohistochemistry; mouse; 1:200; loading ...; fig 4a
MilliporeSigma Drd1 antibody (Sigma, D2944) was used in immunohistochemistry on mouse samples at 1:200 (fig 4a). Nat Commun (2020) ncbi
rat monoclonal (1-1-F11 s.E6)
  • immunohistochemistry - frozen section; human; 1:500; loading ...; fig 2d
  • western blot; human; 1:1000; loading ...; fig 1a
  • immunohistochemistry - frozen section; rat; 1:500; loading ...; fig 2c
  • western blot; rat; 1:1000; loading ...; fig 1a
  • immunohistochemistry knockout validation; mouse; 1:500; loading ...; fig 2a
  • western blot knockout validation; mouse; 1:1000; loading ...; fig 1a
  • immunohistochemistry - frozen section; mouse; 1:500; loading ...; fig 2b
MilliporeSigma Drd1 antibody (Sigma-Aldrich, D2944) was used in immunohistochemistry - frozen section on human samples at 1:500 (fig 2d), in western blot on human samples at 1:1000 (fig 1a), in immunohistochemistry - frozen section on rat samples at 1:500 (fig 2c), in western blot on rat samples at 1:1000 (fig 1a), in immunohistochemistry knockout validation on mouse samples at 1:500 (fig 2a), in western blot knockout validation on mouse samples at 1:1000 (fig 1a) and in immunohistochemistry - frozen section on mouse samples at 1:500 (fig 2b). Amino Acids (2017) ncbi
rat monoclonal (1-1-F11 s.E6)
  • proximity ligation assay; human; fig 6
  • immunohistochemistry knockout validation; mouse; 1:200-1:500; fig 5
  • proximity ligation assay; mouse; fig 6
MilliporeSigma Drd1 antibody (Sigma-Aldrich, D2944) was used in proximity ligation assay on human samples (fig 6), in immunohistochemistry knockout validation on mouse samples at 1:200-1:500 (fig 5) and in proximity ligation assay on mouse samples (fig 6). Mol Psychiatry (2015) ncbi
rat monoclonal (1-1-F11 s.E6)
  • immunohistochemistry - free floating section; mouse; 1:250
  • immunocytochemistry; mouse; 1:200
MilliporeSigma Drd1 antibody (Sigma Aldrich, D2944) was used in immunohistochemistry - free floating section on mouse samples at 1:250 and in immunocytochemistry on mouse samples at 1:200. J Comp Neurol (2015) ncbi
Articles Reviewed
  1. Liu Z, Yang N, Dong J, Tian W, Chang L, Ma J, et al. Deficiency in endocannabinoid synthase DAGLB contributes to early onset Parkinsonism and murine nigral dopaminergic neuron dysfunction. Nat Commun. 2022;13:3490 pubmed publisher
  2. Sun R, Tsunekawa T, Hirose T, Yaginuma H, Taki K, Mizoguchi A, et al. GABAB receptor signaling in the caudate putamen is involved in binge-like consumption during a high fat diet in mice. Sci Rep. 2021;11:19296 pubmed publisher
  3. Ke Y, Weng M, Chhetri G, Usman M, Li Y, Yu Q, et al. Trappc9 deficiency in mice impairs learning and memory by causing imbalance of dopamine D1 and D2 neurons. Sci Adv. 2020;6: pubmed publisher
  4. Eom T, Han S, Kim J, Blundon J, Wang Y, Yu J, et al. Schizophrenia-related microdeletion causes defective ciliary motility and brain ventricle enlargement via microRNA-dependent mechanisms in mice. Nat Commun. 2020;11:912 pubmed publisher
  5. Stojanovic T, Orlova M, Sialana F, Höger H, Stuchlík S, Milenkovic I, et al. Validation of dopamine receptor DRD1 and DRD2 antibodies using receptor deficient mice. Amino Acids. 2017;49:1101-1109 pubmed publisher
  6. Qiu Y, Chen D, Huang X, Huang L, Tang L, Jiang J, et al. Neuroprotective effects of HTR1A antagonist WAY-100635 on scopolamine-induced delirium in rats and underlying molecular mechanisms. BMC Neurosci. 2016;17:66 pubmed
  7. Feng X, Zhang D, Wang Y, Fan R, Hong F, Zhang Y, et al. Dopamine enhances duodenal epithelial permeability via the dopamine D5 receptor in rodent. Acta Physiol (Oxf). 2017;220:113-123 pubmed publisher
  8. Kajiwara M, Ban T, Matsubara K, Nakanishi Y, Masuda S. Urinary Dopamine as a Potential Index of the Transport Activity of Multidrug and Toxin Extrusion in the Kidney. Int J Mol Sci. 2016;17: pubmed publisher
  9. Frank R, Komiyama N, Ryan T, Zhu F, O Dell T, Grant S. NMDA receptors are selectively partitioned into complexes and supercomplexes during synapse maturation. Nat Commun. 2016;7:11264 pubmed publisher
  10. Aher Y, Subramaniyan S, Shanmugasundaram B, Sase A, Saroja S, Holy M, et al. A Novel Heterocyclic Compound CE-104 Enhances Spatial Working Memory in the Radial Arm Maze in Rats and Modulates the Dopaminergic System. Front Behav Neurosci. 2016;10:20 pubmed publisher
  11. Romano López A, Méndez Díaz M, García F, Regalado Santiago C, Ruiz Contreras A, Prospero Garcia O. Maternal separation and early stress cause long-lasting effects on dopaminergic and endocannabinergic systems and alters dendritic morphology in the nucleus accumbens and frontal cortex in rats. Dev Neurobiol. 2016;76:819-31 pubmed publisher
  12. Zhang B, Yang X, Tiberi M. Functional importance of two conserved residues in intracellular loop 1 and transmembrane region 2 of Family A GPCRs: insights from ligand binding and signal transduction responses of D1 and D5 dopaminergic receptor mutants. Cell Signal. 2015;27:2014-25 pubmed publisher
  13. Frederick A, Yano H, Trifilieff P, Vishwasrao H, Biezonski D, Mészáros J, et al. Evidence against dopamine D1/D2 receptor heteromers. Mol Psychiatry. 2015;20:1373-85 pubmed publisher
  14. Biezonski D, Trifilieff P, Meszaros J, Javitch J, Kellendonk C. Evidence for limited D1 and D2 receptor coexpression and colocalization within the dorsal striatum of the neonatal mouse. J Comp Neurol. 2015;523:1175-89 pubmed publisher
  15. Brewer K, Baran C, Whitfield B, Jensen A, Clemens S. Dopamine D3 receptor dysfunction prevents anti-nociceptive effects of morphine in the spinal cord. Front Neural Circuits. 2014;8:62 pubmed publisher
  16. Kurz A, Double K, Lastres Becker I, Tozzi A, Tantucci M, Bockhart V, et al. A53T-alpha-synuclein overexpression impairs dopamine signaling and striatal synaptic plasticity in old mice. PLoS ONE. 2010;5:e11464 pubmed publisher