This is a Validated Antibody Database (VAD) review about rat Dhfr, based on 6 published articles (read how Labome selects the articles), using Dhfr antibody in all methods. It is aimed to help Labome visitors find the most suited Dhfr antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Dhfr synonym: Dhfr1; dihydrofolate reductase; Dihydrofolate reductase 1 (active)

Abnova
mouse monoclonal (2B10)
  • western blot; mouse; 1:500; loading ...; fig 2a
Abnova Dhfr antibody (Novus Biologicals, H00001719-M01) was used in western blot on mouse samples at 1:500 (fig 2a). Redox Biol (2019) ncbi
Santa Cruz Biotechnology
mouse monoclonal (A-9)
  • western blot; human; fig 2
Santa Cruz Biotechnology Dhfr antibody (santa Cruz, sc-377091) was used in western blot on human samples (fig 2). Nat Methods (2015) ncbi
mouse monoclonal (49)
  • western blot; human; 1:200
Santa Cruz Biotechnology Dhfr antibody (Santa Cruz, sc-136246) was used in western blot on human samples at 1:200. BMC Biotechnol (2015) ncbi
Abcam
rabbit monoclonal (EPR5285)
  • western blot; mouse; fig 2
Abcam Dhfr antibody (Abcam, ab124814) was used in western blot on mouse samples (fig 2). Science (2016) ncbi
Proteintech Group
rabbit polyclonal
  • western blot; human; 1:500; loading ...; fig 6f
In order to study the contributions of dihydrofolate reductase and serine hydroxymethyltransferase-2 to the development of rod and ring structures, Proteintech Group Dhfr antibody (Proteintech, 15194-1-AP) was used in western blot on human samples at 1:500 (fig 6f). J Cell Sci (2016) ncbi
BD Biosciences
mouse monoclonal (49/DHFR)
  • western blot; mouse; 1:500; loading ...; fig 4c
BD Biosciences Dhfr antibody (BD Transduction Laboratories, 610697) was used in western blot on mouse samples at 1:500 (fig 4c). Arterioscler Thromb Vasc Biol (2016) ncbi
Articles Reviewed
  1. Li Q, Youn J, Siu K, Murugesan P, Zhang Y, Cai H. Knockout of dihydrofolate reductase in mice induces hypertension and abdominal aortic aneurysm via mitochondrial dysfunction. Redox Biol. 2019;24:101185 pubmed publisher
  2. Calise S, Purich D, Nguyen T, Saleem D, Krueger C, Yin J, et al. 'Rod and ring' formation from IMP dehydrogenase is regulated through the one-carbon metabolic pathway. J Cell Sci. 2016;129:3042-52 pubmed publisher
  3. Ben Sahra I, Hoxhaj G, Ricoult S, Asara J, Manning B. mTORC1 induces purine synthesis through control of the mitochondrial tetrahydrofolate cycle. Science. 2016;351:728-733 pubmed publisher
  4. Xia N, Horke S, Habermeier A, Closs E, Reifenberg G, Gericke A, et al. Uncoupling of Endothelial Nitric Oxide Synthase in Perivascular Adipose Tissue of Diet-Induced Obese Mice. Arterioscler Thromb Vasc Biol. 2016;36:78-85 pubmed publisher
  5. Huber K, Olek K, Müller A, Tan C, Bennett K, Colinge J, et al. Proteome-wide drug and metabolite interaction mapping by thermal-stability profiling. Nat Methods. 2015;12:1055-7 pubmed publisher
  6. Chin C, Chin H, Chin C, Lai E, Ng S. Engineering selection stringency on expression vector for the production of recombinant human alpha1-antitrypsin using Chinese Hamster ovary cells. BMC Biotechnol. 2015;15:44 pubmed publisher