This is a Validated Antibody Database (VAD) review about rat Cyld, based on 18 published articles (read how Labome selects the articles), using Cyld antibody in all methods. It is aimed to help Labome visitors find the most suited Cyld antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Cyld synonym: LRRGT00003; Rp1; Rp1h

Knockout validation
Santa Cruz Biotechnology
mouse monoclonal (E-4)
  • western blot knockout validation; human; 1:200; loading ...; fig 1a
Santa Cruz Biotechnology Cyld antibody (Santa Cruz, sc-74434) was used in western blot knockout validation on human samples at 1:200 (fig 1a). Cancers (Basel) (2020) ncbi
Santa Cruz Biotechnology
mouse monoclonal (E-10)
  • western blot knockout validation; human; 1:500; loading ...; fig 4a
Santa Cruz Biotechnology Cyld antibody (SantaCruz, sc-74435) was used in western blot knockout validation on human samples at 1:500 (fig 4a). Front Cell Dev Biol (2018) ncbi
Santa Cruz Biotechnology
mouse monoclonal (E-4)
  • western blot knockout validation; human; 1:200; loading ...; fig 1a
Santa Cruz Biotechnology Cyld antibody (Santa Cruz, sc-74434) was used in western blot knockout validation on human samples at 1:200 (fig 1a). Cancers (Basel) (2020) ncbi
mouse monoclonal (E-10)
  • western blot; human; loading ...; fig 2d
Santa Cruz Biotechnology Cyld antibody (Santa, sc74435) was used in western blot on human samples (fig 2d). Cell Rep (2019) ncbi
mouse monoclonal (H-6)
  • immunoprecipitation; human; loading ...; fig 1c
  • western blot; human; loading ...; fig 1c, 1d
Santa Cruz Biotechnology Cyld antibody (Santa Cruz, sc-137139) was used in immunoprecipitation on human samples (fig 1c) and in western blot on human samples (fig 1c, 1d). Cell Rep (2019) ncbi
mouse monoclonal (E-10)
  • western blot knockout validation; human; 1:500; loading ...; fig 4a
Santa Cruz Biotechnology Cyld antibody (SantaCruz, sc-74435) was used in western blot knockout validation on human samples at 1:500 (fig 4a). Front Cell Dev Biol (2018) ncbi
mouse monoclonal (E-10)
  • western blot; mouse; loading ...; fig EV2c
  • western blot; human; loading ...; fig EV2b
Santa Cruz Biotechnology Cyld antibody (Santa Cruz Biotechnology, sc-74435) was used in western blot on mouse samples (fig EV2c) and in western blot on human samples (fig EV2b). EMBO J (2017) ncbi
mouse monoclonal (E-10)
  • western blot; human; fig 1
Santa Cruz Biotechnology Cyld antibody (Santa Cruz, sc-74435) was used in western blot on human samples (fig 1). Cell Rep (2016) ncbi
mouse monoclonal (E-10)
  • western blot; mouse; fig 6
In order to demonstrate that OTULIN is essential for preventing TNF-associated systemic inflammation in humans and mice, Santa Cruz Biotechnology Cyld antibody (santa Cruz, sc-74435) was used in western blot on mouse samples (fig 6). Cell (2016) ncbi
mouse monoclonal (E-10)
  • western blot; human; loading ...; fig 4a
In order to show that CARD14 activates p38 and JNK MAP kinase pathways, Santa Cruz Biotechnology Cyld antibody (Santa Cruz, sc-74435) was used in western blot on human samples (fig 4a). EMBO Rep (2016) ncbi
mouse monoclonal (E-10)
  • western blot; mouse; fig 1
Santa Cruz Biotechnology Cyld antibody (Santa Cruz, sc-74435) was used in western blot on mouse samples (fig 1). PLoS ONE (2015) ncbi
mouse monoclonal (E-10)
  • western blot; human; loading ...; fig 5d
Santa Cruz Biotechnology Cyld antibody (Santa Cruz, sc-74435) was used in western blot on human samples (fig 5d). PLoS ONE (2014) ncbi
Novus Biologicals
domestic rabbit polyclonal (OTI2E12)
  • immunocytochemistry; human; 10 ug/ml; fig 4c
  • western blot; human; fig 4d
Novus Biologicals Cyld antibody (Novus, NB110-95574) was used in immunocytochemistry on human samples at 10 ug/ml (fig 4c) and in western blot on human samples (fig 4d). J Biol Chem (2017) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D1A10)
  • western blot; mouse; 1:2000; loading ...; fig 5a
Cell Signaling Technology Cyld antibody (Cell Signaling Technology, 8462) was used in western blot on mouse samples at 1:2000 (fig 5a). J Cell Sci (2019) ncbi
domestic rabbit monoclonal (D1A10)
  • western blot; human; 1:1000; fig s15
Cell Signaling Technology Cyld antibody (Cell Signaling, 8462) was used in western blot on human samples at 1:1000 (fig s15). Nat Commun (2016) ncbi
domestic rabbit monoclonal (D1A10)
  • western blot; human; fig 5
Cell Signaling Technology Cyld antibody (Cell Signaling, 8462) was used in western blot on human samples (fig 5). EMBO J (2016) ncbi
domestic rabbit monoclonal (D1A10)
  • western blot; human; 1:1000; fig s11
Cell Signaling Technology Cyld antibody (Cell Signaling, 8462S) was used in western blot on human samples at 1:1000 (fig s11). Nat Commun (2016) ncbi
domestic rabbit monoclonal (D1A10)
  • western blot; human
Cell Signaling Technology Cyld antibody (Cell Signaling, 8462) was used in western blot on human samples . J Biol Chem (2016) ncbi
domestic rabbit monoclonal (D1A10)
  • western blot; human; fig 3, 4, 5
In order to analyze SUMOylation in neuroblastoma cells as it impairs deubiquitinating activity of CYLD, Cell Signaling Technology Cyld antibody (Cell Signaling, D1A10) was used in western blot on human samples (fig 3, 4, 5). Oncogene (2015) ncbi
domestic rabbit monoclonal (D1A10)
  • western blot; human; 1:1000; fig 2b
Cell Signaling Technology Cyld antibody (Cell Signaling, 8462S) was used in western blot on human samples at 1:1000 (fig 2b). Nat Chem Biol (2014) ncbi
Articles Reviewed
  1. Pseftogas A, Xanthopoulos K, Poutahidis T, Ainali C, Dafou D, Panteris E, et al. The Tumor Suppressor CYLD Inhibits Mammary Epithelial to Mesenchymal Transition by the Coordinated Inhibition of YAP/TAZ and TGF Signaling. Cancers (Basel). 2020;12: pubmed publisher
  2. Gehring T, Erdmann T, Rahm M, Graß C, Flatley A, O Neill T, et al. MALT1 Phosphorylation Controls Activation of T Lymphocytes and Survival of ABC-DLBCL Tumor Cells. Cell Rep. 2019;29:873-888.e10 pubmed publisher
  3. Douanne T, André Grégoire G, Thys A, Trillet K, Gavard J, Bidere N. CYLD Regulates Centriolar Satellites Proteostasis by Counteracting the E3 Ligase MIB1. Cell Rep. 2019;27:1657-1665.e4 pubmed publisher
  4. Wang Z, Feng J, Yu J, Chen G. FKBP12 mediates necroptosis by initiating RIPK1-RIPK3-MLKL signal transduction in response to TNF receptor 1 ligation. J Cell Sci. 2019;132: pubmed publisher
  5. Lork M, Kreike M, Staal J, Beyaert R. Importance of Validating Antibodies and Small Compound Inhibitors Using Genetic Knockout Studies-T Cell Receptor-Induced CYLD Phosphorylation by IKK?/TBK1 as a Case Study. Front Cell Dev Biol. 2018;6:40 pubmed publisher
  6. Wu G, Mu T, Gao Z, Wang J, Sy M, Li C. Prion protein is required for tumor necrosis factor α (TNFα)-triggered nuclear factor κB (NF-κB) signaling and cytokine production. J Biol Chem. 2017;292:18747-18759 pubmed publisher
  7. Lafont E, Kantari Mimoun C, Dráber P, De Miguel D, Hartwig T, Reichert M, et al. The linear ubiquitin chain assembly complex regulates TRAIL-induced gene activation and cell death. EMBO J. 2017;36:1147-1166 pubmed publisher
  8. Nakazawa S, Oikawa D, Ishii R, Ayaki T, Takahashi H, Takeda H, et al. Linear ubiquitination is involved in the pathogenesis of optineurin-associated amyotrophic lateral sclerosis. Nat Commun. 2016;7:12547 pubmed publisher
  9. Kupka S, De Miguel D, Dráber P, Martino L, Surinova S, Rittinger K, et al. SPATA2-Mediated Binding of CYLD to HOIP Enables CYLD Recruitment to Signaling Complexes. Cell Rep. 2016;16:2271-80 pubmed publisher
  10. Damgaard R, Walker J, Marco Casanova P, Morgan N, Titheradge H, Elliott P, et al. The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity. Cell. 2016;166:1215-1230.e20 pubmed publisher
  11. Wagner S, Satpathy S, Beli P, Choudhary C. SPATA2 links CYLD to the TNF-? receptor signaling complex and modulates the receptor signaling outcomes. EMBO J. 2016;35:1868-84 pubmed publisher
  12. Lin W, Zhang J, Lin H, Li Z, Sun X, Xin D, et al. Syndecan-4 negatively regulates antiviral signalling by mediating RIG-I deubiquitination via CYLD. Nat Commun. 2016;7:11848 pubmed publisher
  13. Afonina I, Van Nuffel E, Baudelet G, Driege Y, Kreike M, Staal J, et al. The paracaspase MALT1 mediates CARD14-induced signaling in keratinocytes. EMBO Rep. 2016;17:914-27 pubmed publisher
  14. Monian P, Jiang X. The Cellular Apoptosis Susceptibility Protein (CAS) Promotes Tumor Necrosis Factor-related Apoptosis-inducing Ligand (TRAIL)-induced Apoptosis and Cell Proliferation. J Biol Chem. 2016;291:2379-88 pubmed publisher
  15. Yu J, Hoffman S, Beal A, Dykon A, Ringenberg M, Hughes A, et al. MALT1 Protease Activity Is Required for Innate and Adaptive Immune Responses. PLoS ONE. 2015;10:e0127083 pubmed publisher
  16. Baens M, Bonsignore L, Somers R, Vanderheydt C, Weeks S, Gunnarsson J, et al. MALT1 auto-proteolysis is essential for NF-κB-dependent gene transcription in activated lymphocytes. PLoS ONE. 2014;9:e103774 pubmed publisher
  17. Kobayashi T, Masoumi K, Massoumi R. Deubiquitinating activity of CYLD is impaired by SUMOylation in neuroblastoma cells. Oncogene. 2015;34:2251-60 pubmed publisher
  18. Liang Q, Dexheimer T, Zhang P, Rosenthal A, Villamil M, You C, et al. A selective USP1-UAF1 inhibitor links deubiquitination to DNA damage responses. Nat Chem Biol. 2014;10:298-304 pubmed publisher