This is a Validated Antibody Database (VAD) review about rat Cspg4, based on 24 published articles (read how Labome selects the articles), using Cspg4 antibody in all methods. It is aimed to help Labome visitors find the most suited Cspg4 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Cspg4 synonym: Ng2

Abcam
mouse monoclonal (132.38)
  • immunohistochemistry; mouse; 1:150; loading ...; fig 1c
Abcam Cspg4 antibody (Abcam, ab50009) was used in immunohistochemistry on mouse samples at 1:150 (fig 1c). Nat Commun (2020) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; pigs ; 1:250; loading ...; fig 2f, 2g, 2h
Abcam Cspg4 antibody (Abcam, ab129051) was used in immunohistochemistry - paraffin section on pigs samples at 1:250 (fig 2f, 2g, 2h). PLoS ONE (2020) ncbi
mouse monoclonal (132.38)
  • immunohistochemistry - frozen section; mouse; loading ...; fig 2f
Abcam Cspg4 antibody (Abcam, ab5009) was used in immunohistochemistry - frozen section on mouse samples (fig 2f). PLoS ONE (2019) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; mouse; loading ...; fig s9b
Abcam Cspg4 antibody (Abcam, ab129051) was used in immunocytochemistry on mouse samples (fig s9b). Nature (2019) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:200; loading ...; fig 1e
  • immunocytochemistry; human; 1:200; loading ...; fig s1a
  • western blot; human; loading ...; fig s1b
Abcam Cspg4 antibody (Abcam, ab129051) was used in immunohistochemistry - paraffin section on mouse samples at 1:200 (fig 1e), in immunocytochemistry on human samples at 1:200 (fig s1a) and in western blot on human samples (fig s1b). J Cell Biol (2019) ncbi
mouse monoclonal (132.38)
  • immunohistochemistry - frozen section; mouse; 1:1000; fig 7
Abcam Cspg4 antibody (Abcam, ab50009) was used in immunohistochemistry - frozen section on mouse samples at 1:1000 (fig 7). Glia (2018) ncbi
mouse monoclonal (132.38)
  • immunohistochemistry; rat; 1:100; loading ...; fig s1d
Abcam Cspg4 antibody (Abcam, ab50009) was used in immunohistochemistry on rat samples at 1:100 (fig s1d). Cell Death Differ (2018) ncbi
mouse monoclonal (132.38)
  • western blot; mouse; 1:200; loading ...; fig s5m
In order to identify an astrocyte subtype induced by activated microglia, Abcam Cspg4 antibody (abcam, ab50009) was used in western blot on mouse samples at 1:200 (fig s5m). Nature (2017) ncbi
mouse monoclonal (132.38)
  • immunohistochemistry; mouse; 1:100; fig s13
In order to determine how brain inflammation is recovered by neural stem cells that sustain natural killer cells, Abcam Cspg4 antibody (Abcam, ab50009) was used in immunohistochemistry on mouse samples at 1:100 (fig s13). Nat Neurosci (2016) ncbi
mouse monoclonal (132.38)
  • immunocytochemistry; mouse; fig s1
In order to elucidate the mechanism by which epsilon-toxin causes white matter damage, Abcam Cspg4 antibody (Abcam, ab5009) was used in immunocytochemistry on mouse samples (fig s1). MBio (2015) ncbi
mouse monoclonal (132.38)
  • immunohistochemistry - frozen section; rat; 1:1000; loading ...; fig 3g
Abcam Cspg4 antibody (Abcam, ab50009) was used in immunohistochemistry - frozen section on rat samples at 1:1000 (fig 3g). BMC Neurosci (2015) ncbi
mouse monoclonal (132.38)
  • immunohistochemistry - frozen section; mouse; fig 8
Abcam Cspg4 antibody (Abcam, ab50009) was used in immunohistochemistry - frozen section on mouse samples (fig 8). J Cell Mol Med (2015) ncbi
mouse monoclonal (132.38)
  • immunocytochemistry; human; 1:200
Abcam Cspg4 antibody (Abcam, ab50009) was used in immunocytochemistry on human samples at 1:200. Biochem Biophys Res Commun (2015) ncbi
mouse monoclonal (132.38)
  • immunohistochemistry; mouse; 1:200
Abcam Cspg4 antibody (Abcam, ab50009) was used in immunohistochemistry on mouse samples at 1:200. Neurobiol Dis (2014) ncbi
Invitrogen
mouse monoclonal (D120.43/D4.11/N143.8/N109.6)
  • immunohistochemistry - free floating section; mouse; 1:100; loading ...; fig 3e
Invitrogen Cspg4 antibody (Thermo Fisher, 37-2700) was used in immunohistochemistry - free floating section on mouse samples at 1:100 (fig 3e). elife (2020) ncbi
mouse monoclonal (D120.43/D4.11/N143.8/N109.6)
  • flow cytometry; rat; 5 ug/ml; fig 2
In order to elucidate how CD200 restrains macrophage attacks on oligodendrocyte precursors by tollike receptor 4, Invitrogen Cspg4 antibody (Invitrogen, 37-2700) was used in flow cytometry on rat samples at 5 ug/ml (fig 2). J Cereb Blood Flow Metab (2016) ncbi
mouse monoclonal (N143.8)
  • flow cytometry; human; fig 1
In order to assess the preclinical safety profile of a human embryonic stem cell-derived oligodendrocyte progenitor cell therapy product to treat spinal cord injury, Invitrogen Cspg4 antibody (Invitrogen, 37-C2300) was used in flow cytometry on human samples (fig 1). Regen Med (2015) ncbi
mouse monoclonal (D120.43/D4.11/N143.8/N109.6)
  • immunohistochemistry; rat; fig 4
In order to study the phenotype overlap of different glial cell populations of NG-2 (+), astroglia, and oligodendroglia cells, Invitrogen Cspg4 antibody (Zymed, 37-2700) was used in immunohistochemistry on rat samples (fig 4). Front Neuroanat (2015) ncbi
mouse monoclonal (D120.43/D4.11/N143.8/N109.6)
  • immunohistochemistry - free floating section; rat; 1:300
In order to assess IL-1beta expression in periependymal astrocytes after injury, Invitrogen Cspg4 antibody (Invitrogen, 372700) was used in immunohistochemistry - free floating section on rat samples at 1:300. Neuroscience (2015) ncbi
mouse monoclonal (D120.43/D4.11/N143.8/N109.6)
  • immunohistochemistry - frozen section; rat; 1:300; fig 9
In order to study the expression of Frizzled receptors in the damaged spinal cord, Invitrogen Cspg4 antibody (Zymed, 37-2700) was used in immunohistochemistry - frozen section on rat samples at 1:300 (fig 9). PLoS ONE (2012) ncbi
mouse monoclonal (D120.43/D4.11/N143.8/N109.6)
  • immunohistochemistry; rat; fig 8e
In order to study the luminal/abluminal localization of a number of blood-brain barrier transporters expressed by endothelial cells, Invitrogen Cspg4 antibody (Invitrogen, N109.6) was used in immunohistochemistry on rat samples (fig 8e). Neuroscience (2008) ncbi
Santa Cruz Biotechnology
mouse monoclonal (G-9)
  • western blot; human; 1:500; loading ...; fig 5f
Santa Cruz Biotechnology Cspg4 antibody (Santa Cruz Biotechnology, sc-166251) was used in western blot on human samples at 1:500 (fig 5f). Cells (2020) ncbi
mouse monoclonal (LHM 2)
  • flow cytometry; mouse; 1:20; loading ...; fig 6c
Santa Cruz Biotechnology Cspg4 antibody (Santa Cruz, sc-53389) was used in flow cytometry on mouse samples at 1:20 (fig 6c). J Cell Mol Med (2014) ncbi
mouse monoclonal (132.38)
  • immunohistochemistry - frozen section; rat; 1:1000
In order to characterize the expression of chondroitinase ABC delivery by an adenovirus vector to the central nervous system, Santa Cruz Biotechnology Cspg4 antibody (Santa Cruz, sc33666) was used in immunohistochemistry - frozen section on rat samples at 1:1000. J Neurosci Methods (2014) ncbi
Articles Reviewed
  1. Schmitt B, Boewe A, Becker V, Nalbach L, Gu Y, Götz C, et al. Protein Kinase CK2 Regulates Nerve/Glial Antigen (NG)2-Mediated Angiogenic Activity of Human Pericytes. Cells. 2020;9: pubmed publisher
  2. Sase S, Almad A, Boecker C, Guedes Dias P, Li J, Takanohashi A, et al. TUBB4A mutations result in both glial and neuronal degeneration in an H-ABC leukodystrophy mouse model. elife. 2020;9: pubmed publisher
  3. Wu Z, Parry M, Hou X, Liu M, Wang H, Cain R, et al. Gene therapy conversion of striatal astrocytes into GABAergic neurons in mouse models of Huntington's disease. Nat Commun. 2020;11:1105 pubmed publisher
  4. Swier V, White K, Meyerholz D, Chefdeville A, Khanna R, Sieren J, et al. Validating indicators of CNS disorders in a swine model of neurological disease. PLoS ONE. 2020;15:e0228222 pubmed publisher
  5. Seki M, Furukawa N, Koitabashi N, Obokata M, Conway S, Arakawa H, et al. Periostin-expressing cell-specific transforming growth factor-β inhibition in pulmonary artery prevents pulmonary arterial hypertension. PLoS ONE. 2019;14:e0220795 pubmed publisher
  6. Wimmer R, Leopoldi A, Aichinger M, Wick N, Hantusch B, Novatchkova M, et al. Human blood vessel organoids as a model of diabetic vasculopathy. Nature. 2019;565:505-510 pubmed publisher
  7. Niu F, Liao K, Hu G, Sil S, Callen S, Guo M, et al. Cocaine-induced release of CXCL10 from pericytes regulates monocyte transmigration into the CNS. J Cell Biol. 2019;218:700-721 pubmed publisher
  8. Hamdan H, Patyal P, Kockara N, Wight P. The wmN1 enhancer region in intron 1 is required for expression of human PLP1. Glia. 2018;66:1763-1774 pubmed publisher
  9. Zhang R, Wu Y, Xie F, Zhong Y, Wang Y, Xu M, et al. RGMa mediates reactive astrogliosis and glial scar formation through TGF?1/Smad2/3 signaling after stroke. Cell Death Differ. 2018;25:1503-1516 pubmed publisher
  10. Liddelow S, Guttenplan K, Clarke L, Bennett F, Bohlen C, Schirmer L, et al. Neurotoxic reactive astrocytes are induced by activated microglia. Nature. 2017;541:481-487 pubmed publisher
  11. Liu Q, Sanai N, Jin W, La Cava A, Van Kaer L, Shi F. Neural stem cells sustain natural killer cells that dictate recovery from brain inflammation. Nat Neurosci. 2016;19:243-52 pubmed publisher
  12. Hayakawa K, Pham L, Seo J, Miyamoto N, Maki T, Terasaki Y, et al. CD200 restrains macrophage attack on oligodendrocyte precursors via toll-like receptor 4 downregulation. J Cereb Blood Flow Metab. 2016;36:781-93 pubmed publisher
  13. Priest C, Manley N, Denham J, Wirth E, Lebkowski J. Preclinical safety of human embryonic stem cell-derived oligodendrocyte progenitors supporting clinical trials in spinal cord injury. Regen Med. 2015;10:939-58 pubmed publisher
  14. Alghamdi B, Fern R. Phenotype overlap in glial cell populations: astroglia, oligodendroglia and NG-2(+) cells. Front Neuroanat. 2015;9:49 pubmed publisher
  15. Linden J, Ma Y, Zhao B, Harris J, Rumah K, Schaeren Wiemers N, et al. Clostridium perfringens Epsilon Toxin Causes Selective Death of Mature Oligodendrocytes and Central Nervous System Demyelination. MBio. 2015;6:e02513 pubmed publisher
  16. Raha Chowdhury R, Raha A, Forostyak S, Zhao J, Stott S, Bomford A. Expression and cellular localization of hepcidin mRNA and protein in normal rat brain. BMC Neurosci. 2015;16:24 pubmed publisher
  17. Zeng H, Vaka V, He X, Booz G, Chen J. High-fat diet induces cardiac remodelling and dysfunction: assessment of the role played by SIRT3 loss. J Cell Mol Med. 2015;19:1847-56 pubmed publisher
  18. Parker W, Qu Z, May J. Ascorbic acid transport in brain microvascular pericytes. Biochem Biophys Res Commun. 2015;458:262-7 pubmed publisher
  19. Paniagua Torija B, Arevalo Martin A, Molina Holgado E, Molina Holgado F, Garcia Ovejero D. Spinal cord injury induces a long-lasting upregulation of interleukin-1β in astrocytes around the central canal. Neuroscience. 2015;284:283-9 pubmed publisher
  20. Milesi S, Boussadia B, Plaud C, Catteau M, Rousset M, de Bock F, et al. Redistribution of PDGFR? cells and NG2DsRed pericytes at the cerebrovasculature after status epilepticus. Neurobiol Dis. 2014;71:151-8 pubmed publisher
  21. Schuster A, Klotz M, Schwab T, Di Liddo R, Bertalot T, Schrenk S, et al. Maintenance of the enteric stem cell niche by bacterial lipopolysaccharides? Evidence and perspectives. J Cell Mol Med. 2014;18:1429-43 pubmed publisher
  22. Alves J, Muir E, Andrews M, Ward A, Michelmore N, Dasgupta D, et al. AAV vector-mediated secretion of chondroitinase provides a sensitive tracer for axonal arborisations. J Neurosci Methods. 2014;227:107-20 pubmed publisher
  23. Gonzalez P, Fernández Martos C, González Fernández C, Arenas E, Rodríguez F. Spatio-temporal expression pattern of frizzled receptors after contusive spinal cord injury in adult rats. PLoS ONE. 2012;7:e50793 pubmed publisher
  24. Roberts L, Black D, Raman C, Woodford K, Zhou M, Haggerty J, et al. Subcellular localization of transporters along the rat blood-brain barrier and blood-cerebral-spinal fluid barrier by in vivo biotinylation. Neuroscience. 2008;155:423-38 pubmed publisher