This is a Validated Antibody Database (VAD) review about rat Col4a2, based on 21 published articles (read how Labome selects the articles), using Col4a2 antibody in all methods. It is aimed to help Labome visitors find the most suited Col4a2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Col4a2 synonym: collagen alpha-2(IV) chain; LOW QUALITY PROTEIN: collagen alpha-2(IV) chain; alpha-2 collagen type IV; collagen, type IV, alpha 2; procollagen, type IV, alpha 2

Bio-Rad
rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; 1:200; fig s2h
Bio-Rad Col4a2 antibody (AbD Serotec, 2150-1470) was used in immunohistochemistry - frozen section on mouse samples at 1:200 (fig s2h). Cell (2018) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse; loading ...; fig 1f
Bio-Rad Col4a2 antibody (BioRad, 2150-1470) was used in immunohistochemistry on mouse samples (fig 1f). J Clin Invest (2017) ncbi
rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; 1:50; fig s10i
In order to investigate the similarity between interferon gamma-induced tumor ischemia and physiological blood vessel regression, Bio-Rad Col4a2 antibody (AbD Serotec, 2150-1470) was used in immunohistochemistry - frozen section on mouse samples at 1:50 (fig s10i). Nature (2017) ncbi
rabbit polyclonal
  • immunohistochemistry - free floating section; mouse; 1:300; loading ...; fig 2a
In order to describe a role for sphingosine 1-phosphate receptor-1 in regulating blood brain barrier permeability, Bio-Rad Col4a2 antibody (Serotec, 2150-1470) was used in immunohistochemistry - free floating section on mouse samples at 1:300 (fig 2a). Proc Natl Acad Sci U S A (2017) ncbi
goat polyclonal
  • immunohistochemistry; mouse; 1:50; loading ...; fig 7
In order to study acute kidney injury in mice by noninvasive measurement of renal perfusion and tissue edema using functional MRI, Bio-Rad Col4a2 antibody (Biozol, 1340-01) was used in immunohistochemistry on mouse samples at 1:50 (fig 7). PLoS ONE (2017) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse; 1:200; fig 5e
In order to examine the contribution of vascular smooth muscle cell-derived endosialin to atherosclerosis, Bio-Rad Col4a2 antibody (AbD Serotec, 2150-1470) was used in immunohistochemistry on mouse samples at 1:200 (fig 5e). Arterioscler Thromb Vasc Biol (2017) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse; 1:100; loading ...; fig 7c
In order to find that mice with a dysfunctional fibronectin-synergy motif suffer from surprisingly mild platelet adhesion and bleeding defects due to delayed thrombus formation after vessel injury, Bio-Rad Col4a2 antibody (Bio-Rad, 2150-1470) was used in immunohistochemistry on mouse samples at 1:100 (fig 7c). elife (2017) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse; 1:1000; loading ...; tbl 1
In order to report the relationship between pre-tumor cells and their surrounding stroma in cerebellar tumor medulloblastoma, Bio-Rad Col4a2 antibody (AbD Serotec, 2150-1470) was used in immunohistochemistry on mouse samples at 1:1000 (tbl 1). elife (2016) ncbi
rabbit polyclonal
  • immunocytochemistry; human; 1:300; fig 4a
In order to study the role of endothelial cell exocytosis in cerebral cavernous malformation disease progression, Bio-Rad Col4a2 antibody (AbD Serotec, 2150-1470) was used in immunocytochemistry on human samples at 1:300 (fig 4a). Nat Med (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; 1:100; fig S1
Bio-Rad Col4a2 antibody (AbD Serotec, 2150-1470) was used in immunohistochemistry - frozen section on mouse samples at 1:100 (fig S1). Nat Commun (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - free floating section; mouse; 1:250; fig 3
In order to determine the rescue of VE-PTP-dependent restoration of Tie2 signaling from angiopoietin-2-induced blood-brain barrier compromise and increased stroke size, Bio-Rad Col4a2 antibody (Abd Serotec, 2150-1470) was used in immunohistochemistry - free floating section on mouse samples at 1:250 (fig 3). Acta Neuropathol (2016) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse; 1:400; fig 1c
Bio-Rad Col4a2 antibody (AbD Serotec, 2150-1470) was used in immunohistochemistry on mouse samples at 1:400 (fig 1c). elife (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - free floating section; mouse; fig 4
In order to characterize the rearrangement in the cerebroventricular malformation and cerebrovascular tree due to a tPA deficiency in mice, Bio-Rad Col4a2 antibody (AbD Serotec, 21501470) was used in immunohistochemistry - free floating section on mouse samples (fig 4). Front Cell Neurosci (2015) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 1c
Bio-Rad Col4a2 antibody (Abd Serotec, 2150-1470) was used in immunohistochemistry - paraffin section on mouse samples at 1:100 (fig 1c). Nat Commun (2015) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse; 1:200
In order to study blood vessel polarization during angiogenic sprouting, Bio-Rad Col4a2 antibody (AbD Serotec, 2150-1470) was used in immunohistochemistry on mouse samples at 1:200. Development (2014) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse; 1:200
In order to determine if ephrin-A5 regulates primary vitreous regression, Bio-Rad Col4a2 antibody (AbD Serotec, 2150-1470) was used in immunohistochemistry on mouse samples at 1:200. Invest Ophthalmol Vis Sci (2014) ncbi
EMD Millipore
mouse monoclonal (23IIC3)
  • other; pig; 1:1000; fig 2
In order to learn about dependence on composition and assembly of endogenous extracellular matrices by blood-brain barrier properties in vitro, EMD Millipore Col4a2 antibody (Millipore, MAB1910) was used in other on pig samples at 1:1000 (fig 2). Cell Tissue Res (2016) ncbi
mouse monoclonal (23IIC3)
  • immunohistochemistry; rat; 1:250
EMD Millipore Col4a2 antibody (Millipore, MAB1910) was used in immunohistochemistry on rat samples at 1:250. PLoS ONE (2014) ncbi
mouse monoclonal (23IIC3)
  • immunohistochemistry - frozen section; human
EMD Millipore Col4a2 antibody (Millipore, MAB1910) was used in immunohistochemistry - frozen section on human samples . Cell Tissue Bank (2014) ncbi
mouse monoclonal (23IIC3)
  • ELISA; human
EMD Millipore Col4a2 antibody (Chemicon International, MAB1910) was used in ELISA on human samples . BMC Cancer (2013) ncbi
mouse monoclonal (23IIC3)
  • immunohistochemistry - free floating section; human; 1:800
In order to use skin biopsy to study human facial cutaneous innervation, EMD Millipore Col4a2 antibody (Chemicon, MAB1910) was used in immunohistochemistry - free floating section on human samples at 1:800. J Anat (2013) ncbi
Articles Reviewed
  1. Dias D, Kim H, Holl D, Werne Solnestam B, Lundeberg J, Carlén M, et al. Reducing Pericyte-Derived Scarring Promotes Recovery after Spinal Cord Injury. Cell. 2018;173:153-165.e22 pubmed publisher
  2. Kim J, Kim Y, Kim J, Park D, Bae H, Lee D, et al. YAP/TAZ regulates sprouting angiogenesis and vascular barrier maturation. J Clin Invest. 2017;127:3441-3461 pubmed publisher
  3. Kammertoens T, Friese C, Arina A, Idel C, Briesemeister D, Rothe M, et al. Tumour ischaemia by interferon-? resembles physiological blood vessel regression. Nature. 2017;545:98-102 pubmed publisher
  4. Yanagida K, Liu C, Faraco G, Galvani S, Smith H, Burg N, et al. Size-selective opening of the blood-brain barrier by targeting endothelial sphingosine 1-phosphate receptor 1. Proc Natl Acad Sci U S A. 2017;114:4531-4536 pubmed publisher
  5. Tewes S, Gueler F, Chen R, Gutberlet M, Jang M, Meier M, et al. Functional MRI for characterization of renal perfusion impairment and edema formation due to acute kidney injury in different mouse strains. PLoS ONE. 2017;12:e0173248 pubmed publisher
  6. Hasanov Z, Ruckdeschel T, König C, Mogler C, Kapel S, Korn C, et al. Endosialin Promotes Atherosclerosis Through Phenotypic Remodeling of Vascular Smooth Muscle Cells. Arterioscler Thromb Vasc Biol. 2017;37:495-505 pubmed publisher
  7. Benito Jardón M, Klapproth S, Gimeno LLuch I, Petzold T, Bharadwaj M, Müller D, et al. The fibronectin synergy site re-enforces cell adhesion and mediates a crosstalk between integrin classes. elife. 2017;6: pubmed publisher
  8. Bassett E, Tokarew N, Allemano E, Mazerolle C, Morin K, Mears A, et al. Norrin/Frizzled4 signalling in the preneoplastic niche blocks medulloblastoma initiation. elife. 2016;5: pubmed publisher
  9. Jenny Zhou H, Qin L, Zhang H, Tang W, Ji W, He Y, et al. Endothelial exocytosis of angiopoietin-2 resulting from CCM3 deficiency contributes to cerebral cavernous malformation. Nat Med. 2016;22:1033-1042 pubmed publisher
  10. Chen Q, Zhang H, Liu Y, Adams S, Eilken H, Stehling M, et al. Endothelial cells are progenitors of cardiac pericytes and vascular smooth muscle cells. Nat Commun. 2016;7:12422 pubmed publisher
  11. Zobel K, Hansen U, Galla H. Blood-brain barrier properties in vitro depend on composition and assembly of endogenous extracellular matrices. Cell Tissue Res. 2016;365:233-45 pubmed publisher
  12. Gurnik S, Devraj K, Macas J, Yamaji M, Starke J, Scholz A, et al. Angiopoietin-2-induced blood-brain barrier compromise and increased stroke size are rescued by VE-PTP-dependent restoration of Tie2 signaling. Acta Neuropathol. 2016;131:753-73 pubmed publisher
  13. Franco C, Jones M, Bernabeu M, Vion A, Barbacena P, Fan J, et al. Non-canonical Wnt signalling modulates the endothelial shear stress flow sensor in vascular remodelling. elife. 2016;5:e07727 pubmed publisher
  14. Stefanitsch C, Lawrence A, Olverling A, Nilsson I, Fredriksson L. tPA Deficiency in Mice Leads to Rearrangement in the Cerebrovascular Tree and Cerebroventricular Malformations. Front Cell Neurosci. 2015;9:456 pubmed publisher
  15. Salvucci O, Ohnuki H, Maric D, Hou X, Li X, Yoon S, et al. EphrinB2 controls vessel pruning through STAT1-JNK3 signalling. Nat Commun. 2015;6:6576 pubmed publisher
  16. Pelton J, Wright C, Leitges M, Bautch V. Multiple endothelial cells constitute the tip of developing blood vessels and polarize to promote lumen formation. Development. 2014;141:4121-6 pubmed publisher
  17. Carlos C, Sonehara N, Oliani S, Burdmann E. Predictive usefulness of urinary biomarkers for the identification of cyclosporine A-induced nephrotoxicity in a rat model. PLoS ONE. 2014;9:e103660 pubmed publisher
  18. Son A, Sheleg M, Cooper M, Sun Y, Kleiman N, Zhou R. Formation of persistent hyperplastic primary vitreous in ephrin-A5-/- mice. Invest Ophthalmol Vis Sci. 2014;55:1594-606 pubmed publisher
  19. Tauzin H, Rolin G, Viennet C, Saas P, Humbert P, Muret P. A skin substitute based on human amniotic membrane. Cell Tissue Bank. 2014;15:257-65 pubmed publisher
  20. Ohlund D, Franklin O, Lundberg E, Lundin C, Sund M. Type IV collagen stimulates pancreatic cancer cell proliferation, migration, and inhibits apoptosis through an autocrine loop. BMC Cancer. 2013;13:154 pubmed publisher
  21. Nolano M, Provitera V, Caporaso G, Stancanelli A, Leandri M, Biasiotta A, et al. Cutaneous innervation of the human face as assessed by skin biopsy. J Anat. 2013;222:161-9 pubmed publisher