This is a Validated Antibody Database (VAD) review about rat Cdk5, based on 37 published articles (read how Labome selects the articles), using Cdk5 antibody in all methods. It is aimed to help Labome visitors find the most suited Cdk5 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Knockout validation
Santa Cruz Biotechnology
mouse monoclonal (DC 17)
  • western blot knockout validation; human; 1:1000; loading ...; fig 1f
Santa Cruz Biotechnology Cdk5 antibody (Santa Cruz, sc-249) was used in western blot knockout validation on human samples at 1:1000 (fig 1f). Nat Commun (2019) ncbi
Invitrogen
mouse monoclonal (DC34)
  • immunocytochemistry knockout validation; mouse; fig 2
In order to demonstrate that the Cdk5-Foxc2 interaction regulates lymphatic vessel development, Invitrogen Cdk5 antibody (Life Technologies, AHZ0492) was used in immunocytochemistry knockout validation on mouse samples (fig 2). Nat Commun (2015) ncbi
Santa Cruz Biotechnology
mouse monoclonal (J-3)
  • western blot; rat; 1:1000; loading ...; fig 5a
Santa Cruz Biotechnology Cdk5 antibody (Santa cruz, sc-6247) was used in western blot on rat samples at 1:1000 (fig 5a). elife (2021) ncbi
mouse monoclonal (J-3)
  • western blot; rat; 1:1000; loading ...; fig 5a
Santa Cruz Biotechnology Cdk5 antibody (Santa cruz, sc-6247) was used in western blot on rat samples at 1:1000 (fig 5a). Mod Pathol (2021) ncbi
mouse monoclonal (DC 17)
  • western blot knockout validation; human; 1:1000; loading ...; fig 1f
Santa Cruz Biotechnology Cdk5 antibody (Santa Cruz, sc-249) was used in western blot knockout validation on human samples at 1:1000 (fig 1f). Nat Commun (2019) ncbi
mouse monoclonal (DC 17)
  • western blot; mouse; 1:5000; loading ...; fig 1c
  • western blot; human; 1:5000; loading ...; fig s1c
In order to study the function of liprin alpha 1 phosphorylation in synapse development, Santa Cruz Biotechnology Cdk5 antibody (Santa Cruz Biotechnology, DC-17) was used in western blot on mouse samples at 1:5000 (fig 1c) and in western blot on human samples at 1:5000 (fig s1c). Proc Natl Acad Sci U S A (2017) ncbi
mouse monoclonal (J-3)
  • western blot; mouse; 1:1000; loading ...; fig ev2
In order to describe the role of miR-132 in amyloid and TAU pathology associated with Alzheimer's disease, Santa Cruz Biotechnology Cdk5 antibody (Santa Cruz, sc-6247) was used in western blot on mouse samples at 1:1000 (fig ev2). EMBO Mol Med (2016) ncbi
mouse monoclonal (DC 17)
  • western blot; human; 1:2000; fig 4
Santa Cruz Biotechnology Cdk5 antibody (Santa Cruz Biotech, sc-249) was used in western blot on human samples at 1:2000 (fig 4). J Korean Med Sci (2016) ncbi
mouse monoclonal (J-3)
  • western blot; human; fig 4
Santa Cruz Biotechnology Cdk5 antibody (Santa Cruz, sc-6247) was used in western blot on human samples (fig 4). Acta Neuropathol Commun (2016) ncbi
mouse monoclonal (DC 17)
  • other; human; loading ...; fig st1
In order to use size exclusion chromatography-microsphere-based affinity proteomics to study clinical samples obtained from pediatric acute leukemia patients, Santa Cruz Biotechnology Cdk5 antibody (SCBT, DC 17) was used in other on human samples (fig st1). Mol Cell Proteomics (2016) ncbi
mouse monoclonal (DC 17)
  • western blot; mouse; 1:1000; fig 2
  • western blot; rat; fig 3
In order to report that p35 S-nitrosylation by nitric oxide signaling regulates hippocampal synaptic strength, Santa Cruz Biotechnology Cdk5 antibody (Santa Cruz, DC17) was used in western blot on mouse samples at 1:1000 (fig 2) and in western blot on rat samples (fig 3). Nat Commun (2015) ncbi
mouse monoclonal (J-3)
  • immunoprecipitation; rat
  • western blot; rat
Santa Cruz Biotechnology Cdk5 antibody (Santa Cruz, sc-6247) was used in immunoprecipitation on rat samples and in western blot on rat samples . PLoS ONE (2015) ncbi
mouse monoclonal (J-3)
  • western blot; human; fig 7
Santa Cruz Biotechnology Cdk5 antibody (Santa Cruz, sc-6247) was used in western blot on human samples (fig 7). Nat Neurosci (2015) ncbi
mouse monoclonal (DC 17)
  • immunoprecipitation; human; fig 1
In order to identify CDK4 binding partners, Santa Cruz Biotechnology Cdk5 antibody (Santa Cruz, DC-17) was used in immunoprecipitation on human samples (fig 1). Cell Cycle (2014) ncbi
mouse monoclonal (J-3)
  • immunohistochemistry - paraffin section; human; 1:200
  • immunoprecipitation; human
  • western blot; human
Santa Cruz Biotechnology Cdk5 antibody (Santa Cruz Biotechnology, sc-6247) was used in immunohistochemistry - paraffin section on human samples at 1:200, in immunoprecipitation on human samples and in western blot on human samples . J Cell Biol (2014) ncbi
mouse monoclonal (DC 17)
  • western blot; human; 1:2000; fig 7c
In order to study the initiation and regulation of radial migration, Santa Cruz Biotechnology Cdk5 antibody (Santa, sc-249) was used in western blot on human samples at 1:2000 (fig 7c). Nat Commun (2014) ncbi
mouse monoclonal (J-3)
  • immunocytochemistry; mouse; 1:100; fig 4
In order to elucidate the molecular mechanisms of dilation formation and nuclear elongation, Santa Cruz Biotechnology Cdk5 antibody (Santa Cruz, sc-6247) was used in immunocytochemistry on mouse samples at 1:100 (fig 4). Development (2014) ncbi
mouse monoclonal (J-3)
  • immunoprecipitation; human; fig 1
  • western blot; human; fig 1
Santa Cruz Biotechnology Cdk5 antibody (Santa Cruz, sc-6247) was used in immunoprecipitation on human samples (fig 1) and in western blot on human samples (fig 1). Exp Cell Res (2015) ncbi
mouse monoclonal (DC 17)
  • western blot; mouse
Santa Cruz Biotechnology Cdk5 antibody (Santa Cruz Biotechnology, DC17) was used in western blot on mouse samples . J Neurosci (2014) ncbi
mouse monoclonal (J-3)
  • immunocytochemistry; human
  • western blot; human
  • western blot; rat
In order to investigate the role of MiR-26b in cell cycle entry, tau-phosphorylation, and apoptosis in postmitotic neurons, Santa Cruz Biotechnology Cdk5 antibody (Santa Cruz Biotechnology, sc-6247) was used in immunocytochemistry on human samples , in western blot on human samples and in western blot on rat samples . J Neurosci (2013) ncbi
Abcam
domestic rabbit monoclonal (EP715Y)
  • western blot; human; 1:1000; fig s4h
Abcam Cdk5 antibody (Abcam, ab40773) was used in western blot on human samples at 1:1000 (fig s4h). Nat Commun (2021) ncbi
domestic rabbit monoclonal (EP715Y)
  • western blot; mouse; 1:500; loading ...; fig 2d
Abcam Cdk5 antibody (Abcam, Ab40773) was used in western blot on mouse samples at 1:500 (fig 2d). Kidney Blood Press Res (2018) ncbi
domestic rabbit monoclonal (EP715Y)
  • western blot; human; 1:1000; fig 6
Abcam Cdk5 antibody (Abcam, ab40773) was used in western blot on human samples at 1:1000 (fig 6). Sci Rep (2015) ncbi
domestic rabbit monoclonal (EP715Y)
  • western blot; rat; 1:2000
Abcam Cdk5 antibody (Abcam, ab40773) was used in western blot on rat samples at 1:2000. Brain Res Bull (2014) ncbi
domestic rabbit monoclonal (EP715Y)
  • immunohistochemistry - frozen section; rat; 1:200
  • immunoprecipitation; rat
  • western blot; rat
Abcam Cdk5 antibody (Abcam, ab40773) was used in immunohistochemistry - frozen section on rat samples at 1:200, in immunoprecipitation on rat samples and in western blot on rat samples . PLoS ONE (2012) ncbi
Invitrogen
mouse monoclonal (DC34)
  • western blot; human; 1:500; loading ...; fig 6s1d
Invitrogen Cdk5 antibody (Thermofisher, AHZ0492) was used in western blot on human samples at 1:500 (fig 6s1d). elife (2020) ncbi
mouse monoclonal (DC34)
  • immunohistochemistry - paraffin section; human; 1:100; loading ...; fig 1b
  • western blot; human; loading ...; fig 2a
In order to find a Cdk5-Foxo1-Bim pathway in cell death in tumorspheres, implicating Cdk5 as a target for tumor-initiating cells, Invitrogen Cdk5 antibody (Life Technologies, AHZ0492) was used in immunohistochemistry - paraffin section on human samples at 1:100 (fig 1b) and in western blot on human samples (fig 2a). Br J Cancer (2017) ncbi
mouse monoclonal (DC34)
  • western blot; human; fig 5
In order to characterize promotion of non-productive angiogenesis by inhibition of endothelial Cdk5 that reduces tumor growth, Invitrogen Cdk5 antibody (Life technologies, AHZ0492) was used in western blot on human samples (fig 5). Oncotarget (2016) ncbi
mouse monoclonal (DC34)
  • immunocytochemistry knockout validation; mouse; fig 2
In order to demonstrate that the Cdk5-Foxc2 interaction regulates lymphatic vessel development, Invitrogen Cdk5 antibody (Life Technologies, AHZ0492) was used in immunocytochemistry knockout validation on mouse samples (fig 2). Nat Commun (2015) ncbi
mouse monoclonal (DC34)
  • western blot; human; 1:100; fig 1
In order to demonstrate that cyclin-dependent kinase 2 and 5 regulate neoangiogenesis, Invitrogen Cdk5 antibody (Molecular Probes, AHZ0492) was used in western blot on human samples at 1:100 (fig 1). J Cell Physiol (2013) ncbi
mouse monoclonal (DC34)
  • western blot; mouse; 1:1000; fig 6
In order to compare the anti-amyloidogenic, anti-inflammatory, and anti-tangle effects of dietary aged garlic extract with its prominent constituents in a mouse model of Alzheimer's disease, Invitrogen Cdk5 antibody (BioSource, AHZ0492) was used in western blot on mouse samples at 1:1000 (fig 6). J Ethnopharmacol (2006) ncbi
OriGene
mouse monoclonal (OTI2H6)
  • immunohistochemistry - frozen section; mouse; 1:20; loading ...; fig 4f
  • immunoprecipitation; mouse; 1:50; loading ...; fig 1c, 4a, 4c
  • immunohistochemistry; mouse; 1:20; loading ...; fig 3a
  • western blot; mouse; 1:500; loading ...; fig 1b, 4a, 4e
  • immunoprecipitation; human; 1:50; loading ...; fig 4b
  • western blot; human; 1:500; loading ...; fig 4b
OriGene Cdk5 antibody (Origene, CF500397) was used in immunohistochemistry - frozen section on mouse samples at 1:20 (fig 4f), in immunoprecipitation on mouse samples at 1:50 (fig 1c, 4a, 4c), in immunohistochemistry on mouse samples at 1:20 (fig 3a), in western blot on mouse samples at 1:500 (fig 1b, 4a, 4e), in immunoprecipitation on human samples at 1:50 (fig 4b) and in western blot on human samples at 1:500 (fig 4b). elife (2019) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig 3b
Cell Signaling Technology Cdk5 antibody (Cell Signaling, 2506) was used in western blot on mouse samples (fig 3b). Nature (2019) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig s6e
In order to search for signaling pathways that modulate polyglutamine-androgen receptor phosphorylation for therapy development, Cell Signaling Technology Cdk5 antibody (Cell Signaling, 2506) was used in western blot on human samples (fig s6e). Sci Transl Med (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:2500; loading ...; fig s2b
In order to demonstrate that microtubule affinity-regulating kinase is a target of methylene blue, Cell Signaling Technology Cdk5 antibody (Cell Signaling, 2506) was used in western blot on human samples at 1:2500 (fig s2b). Sci Rep (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; fig 5a
Cell Signaling Technology Cdk5 antibody (CST, 2506) was used in western blot on human samples (fig 5a). Mol Brain (2016) ncbi
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig s2b
Cell Signaling Technology Cdk5 antibody (Cell Signaling Technology, 2506) was used in western blot on mouse samples (fig s2b). Proc Natl Acad Sci U S A (2016) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; fig 4
In order to elucidate modification microglial phenotype and neuroprotection in P301S tau transgenic mice via TREM2, Cell Signaling Technology Cdk5 antibody (Cell Signaling, 2506) was used in western blot on mouse samples at 1:1000 (fig 4). Neuropharmacology (2016) ncbi
domestic rabbit polyclonal
  • western blot; rat; fig 2c
Cell Signaling Technology Cdk5 antibody (Cell Signaling, 2506) was used in western blot on rat samples (fig 2c). J Diabetes Res (2016) ncbi
Articles Reviewed
  1. Hu D, Sun X, Magpusao A, Fedorov Y, Thompson M, Wang B, et al. Small-molecule suppression of calpastatin degradation reduces neuropathology in models of Huntington's disease. Nat Commun. 2021;12:5305 pubmed publisher
  2. Zhang X, Zou L, Meng L, Xiong M, Pan L, Chen G, et al. Amphiphysin I cleavage by asparagine endopeptidase leads to tau hyperphosphorylation and synaptic dysfunction. elife. 2021;10: pubmed publisher
  3. Chatrian A, Colling R, Browning L, Alham N, Sirinukunwattana K, Malacrino S, et al. Artificial intelligence for advance requesting of immunohistochemistry in diagnostically uncertain prostate biopsies. Mod Pathol. 2021;: pubmed publisher
  4. Vevea J, Chapman E. Acute disruption of the synaptic vesicle membrane protein synaptotagmin 1 using knockoff in mouse hippocampal neurons. elife. 2020;9: pubmed publisher
  5. Brenna A, Olejniczak I, Chavan R, Ripperger J, Langmesser S, Cameroni E, et al. Cyclin-dependent kinase 5 (CDK5) regulates the circadian clock. elife. 2019;8: pubmed publisher
  6. Faraco G, Hochrainer K, Segarra S, Schaeffer S, Santisteban M, Menon A, et al. Dietary salt promotes cognitive impairment through tau phosphorylation. Nature. 2019;: pubmed publisher
  7. Sang Y, Li Y, Zhang Y, Alvarez A, Yu B, Zhang W, et al. CDK5-dependent phosphorylation and nuclear translocation of TRIM59 promotes macroH2A1 ubiquitination and tumorigenicity. Nat Commun. 2019;10:4013 pubmed publisher
  8. Yang X, Ding Y, Yang M, Yu L, Hu Y, Deng Y. Nestin Improves Preeclampsia-Like Symptoms by Inhibiting Activity of Cyclin-Dependent Kinase 5. Kidney Blood Press Res. 2018;43:616-627 pubmed publisher
  9. Huang H, Lin X, Liang Z, Zhao T, Du S, Loy M, et al. Cdk5-dependent phosphorylation of liprin?1 mediates neuronal activity-dependent synapse development. Proc Natl Acad Sci U S A. 2017;114:E6992-E7001 pubmed publisher
  10. Mandl M, Zhang S, Ulrich M, Schmoeckel E, Mayr D, Vollmar A, et al. Inhibition of Cdk5 induces cell death of tumor-initiating cells. Br J Cancer. 2017;116:912-922 pubmed publisher
  11. Polanco M, Parodi S, Piol D, Stack C, Chivet M, Contestabile A, et al. Adenylyl cyclase activating polypeptide reduces phosphorylation and toxicity of the polyglutamine-expanded androgen receptor in spinobulbar muscular atrophy. Sci Transl Med. 2016;8:370ra181 pubmed publisher
  12. Sun W, Lee S, Huang X, Liu S, Inayathullah M, Kim K, et al. Attenuation of synaptic toxicity and MARK4/PAR1-mediated Tau phosphorylation by methylene blue for Alzheimer's disease treatment. Sci Rep. 2016;6:34784 pubmed publisher
  13. Xing H, Lim Y, Chong J, Lee J, Aarsland D, Ballard C, et al. Increased phosphorylation of collapsin response mediator protein-2 at Thr514 correlates with ?-amyloid burden and synaptic deficits in Lewy body dementias. Mol Brain. 2016;9:84 pubmed publisher
  14. Salta E, Sierksma A, Vanden Eynden E, De Strooper B. miR-132 loss de-represses ITPKB and aggravates amyloid and TAU pathology in Alzheimer's brain. EMBO Mol Med. 2016;8:1005-18 pubmed publisher
  15. Xiang J, Guo S, Jiang S, Xu Y, Li J, Li L, et al. Silencing of Long Non-Coding RNA MALAT1 Promotes Apoptosis of Glioma Cells. J Korean Med Sci. 2016;31:688-94 pubmed publisher
  16. Kurbatskaya K, Phillips E, Croft C, Dentoni G, Hughes M, Wade M, et al. Upregulation of calpain activity precedes tau phosphorylation and loss of synaptic proteins in Alzheimer's disease brain. Acta Neuropathol Commun. 2016;4:34 pubmed publisher
  17. Seneviratne U, Nott A, Bhat V, Ravindra K, Wishnok J, Tsai L, et al. S-nitrosation of proteins relevant to Alzheimer's disease during early stages of neurodegeneration. Proc Natl Acad Sci U S A. 2016;113:4152-7 pubmed publisher
  18. Jiang T, Zhang Y, Chen Q, Gao Q, Zhu X, Zhou J, et al. TREM2 modifies microglial phenotype and provides neuroprotection in P301S tau transgenic mice. Neuropharmacology. 2016;105:196-206 pubmed publisher
  19. Draney C, Hobson A, Grover S, Jack B, Tessem J. Cdk5r1 Overexpression Induces Primary β-Cell Proliferation. J Diabetes Res. 2016;2016:6375804 pubmed publisher
  20. Kanderová V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, et al. High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016;15:1246-61 pubmed publisher
  21. Merk H, Zhang S, Lehr T, Müller C, Ulrich M, Bibb J, et al. Inhibition of endothelial Cdk5 reduces tumor growth by promoting non-productive angiogenesis. Oncotarget. 2016;7:6088-104 pubmed publisher
  22. Zhang P, Fu W, Fu A, Ip N. S-nitrosylation-dependent proteasomal degradation restrains Cdk5 activity to regulate hippocampal synaptic strength. Nat Commun. 2015;6:8665 pubmed publisher
  23. Liebl J, Zhang S, Moser M, Agalarov Y, Demir C, Hager B, et al. Cdk5 controls lymphatic vessel development and function by phosphorylation of Foxc2. Nat Commun. 2015;6:7274 pubmed publisher
  24. Yu H, Xie J, Li B, Sun Y, Gao Q, Ding Z, et al. TIGAR regulates DNA damage and repair through pentosephosphate pathway and Cdk5-ATM pathway. Sci Rep. 2015;5:9853 pubmed publisher
  25. Kim B, Serebreni L, Fallica J, Hamdan O, Wang L, Johnston L, et al. Cyclin-dependent kinase five mediates activation of lung xanthine oxidoreductase in response to hypoxia. PLoS ONE. 2015;10:e0124189 pubmed publisher
  26. Xie Q, Wu Q, Horbinski C, Flavahan W, Yang K, Zhou W, et al. Mitochondrial control by DRP1 in brain tumor initiating cells. Nat Neurosci. 2015;18:501-10 pubmed publisher
  27. Jirawatnotai S, Sharma S, Michowski W, Suktitipat B, Geng Y, Quackenbush J, et al. The cyclin D1-CDK4 oncogenic interactome enables identification of potential novel oncogenes and clinical prognosis. Cell Cycle. 2014;13:2889-900 pubmed publisher
  28. Tripathi B, Qian X, Mertins P, Wang D, Papageorge A, Carr S, et al. CDK5 is a major regulator of the tumor suppressor DLC1. J Cell Biol. 2014;207:627-42 pubmed publisher
  29. Ye T, Ip J, Fu A, Ip N. Cdk5-mediated phosphorylation of RapGEF2 controls neuronal migration in the developing cerebral cortex. Nat Commun. 2014;5:4826 pubmed publisher
  30. Nishimura Y, Shikanai M, Hoshino M, Ohshima T, Nabeshima Y, Mizutani K, et al. Cdk5 and its substrates, Dcx and p27kip1, regulate cytoplasmic dilation formation and nuclear elongation in migrating neurons. Development. 2014;141:3540-50 pubmed publisher
  31. Tripathi B, Lowy D, Zelenka P. The Cdk5 activator P39 specifically links muskelin to myosin II and regulates stress fiber formation and actin organization in lens. Exp Cell Res. 2015;330:186-98 pubmed publisher
  32. Tang J, Ip J, Ye T, Ng Y, Yung W, Wu Z, et al. Cdk5-dependent Mst3 phosphorylation and activity regulate neuronal migration through RhoA inhibition. J Neurosci. 2014;34:7425-36 pubmed publisher
  33. Liu X, Liu Y, Zhang J, Zhang W, Sun Y, Gu X, et al. Intrathecal administration of roscovitine prevents remifentanil-induced postoperative hyperalgesia and decreases the phosphorylation of N-methyl-D-aspartate receptor and metabotropic glutamate receptor 5 in spinal cord. Brain Res Bull. 2014;106:9-16 pubmed publisher
  34. Absalon S, Kochanek D, Raghavan V, Krichevsky A. MiR-26b, upregulated in Alzheimer's disease, activates cell cycle entry, tau-phosphorylation, and apoptosis in postmitotic neurons. J Neurosci. 2013;33:14645-59 pubmed publisher
  35. Zhang H, Zhang X, Wang W, Xue Q, Lu H, Huang J, et al. Increased synaptophysin is involved in inflammation-induced heat hyperalgesia mediated by cyclin-dependent kinase 5 in rats. PLoS ONE. 2012;7:e46666 pubmed publisher
  36. de Nigris F, Mancini F, Schiano C, Infante T, Zullo A, Minucci P, et al. Osteosarcoma cells induce endothelial cell proliferation during neo-angiogenesis. J Cell Physiol. 2013;228:846-52 pubmed publisher
  37. Chauhan N. Effect of aged garlic extract on APP processing and tau phosphorylation in Alzheimer's transgenic model Tg2576. J Ethnopharmacol. 2006;108:385-94 pubmed