This is a Validated Antibody Database (VAD) review about rat Cdc20, based on 28 published articles (read how Labome selects the articles), using Cdc20 antibody in all methods. It is aimed to help Labome visitors find the most suited Cdc20 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Cdc20 synonym: p55cdc; cell division cycle protein 20 homolog; cell cycle protein p55CDC; cell division cycle 20 homolog

Santa Cruz Biotechnology
mouse monoclonal (E-7)
  • western blot; human; 1:1000; loading ...; fig s3a
Santa Cruz Biotechnology Cdc20 antibody (Santa Cruz, sc-13162) was used in western blot on human samples at 1:1000 (fig s3a). EMBO J (2018) ncbi
mouse monoclonal (E-7)
  • western blot; human; loading ...; fig 1a
Santa Cruz Biotechnology Cdc20 antibody (SantaCruz, sc-13162) was used in western blot on human samples (fig 1a). Nature (2018) ncbi
mouse monoclonal (E-7)
  • immunoprecipitation; human; loading ...; fig 1e
  • western blot; human; loading ...; fig 1e
Santa Cruz Biotechnology Cdc20 antibody (Santa Cruz Biotechnology, sc-13162) was used in immunoprecipitation on human samples (fig 1e) and in western blot on human samples (fig 1e). Nat Cell Biol (2017) ncbi
mouse monoclonal (E-7)
  • proximity ligation assay; human; 1:250; loading ...; fig s1d
In order to examine if the CDC20-MAD2 complex is cell cycle regulated, Santa Cruz Biotechnology Cdc20 antibody (Santa Cruz, sc-13162) was used in proximity ligation assay on human samples at 1:250 (fig s1d). Sci Rep (2017) ncbi
mouse monoclonal (H-7)
  • western blot; human; 1:100; loading ...; fig 1c
Santa Cruz Biotechnology Cdc20 antibody (SantaCruz Biotechnology, sc-5296) was used in western blot on human samples at 1:100 (fig 1c). Biol Open (2016) ncbi
mouse monoclonal (E-7)
  • immunoprecipitation; human; loading ...; fig 1d
  • western blot; human; loading ...; fig 1b
Santa Cruz Biotechnology Cdc20 antibody (Santa Cruz, sc-13162) was used in immunoprecipitation on human samples (fig 1d) and in western blot on human samples (fig 1b). Cell Cycle (2016) ncbi
mouse monoclonal (E-7)
  • ELISA; human; 1:1000; fig 1c
  • western blot; human; 1:1000
Santa Cruz Biotechnology Cdc20 antibody (Santa Cruz, sc-13162) was used in ELISA on human samples at 1:1000 (fig 1c) and in western blot on human samples at 1:1000. MAbs (2016) ncbi
mouse monoclonal (E-7)
  • western blot; human; 1:1000; fig 1
Santa Cruz Biotechnology Cdc20 antibody (Santa Cruz, sc-13162) was used in western blot on human samples at 1:1000 (fig 1). Nat Commun (2016) ncbi
mouse monoclonal (H-7)
  • western blot; human; 1:200; fig 3
Santa Cruz Biotechnology Cdc20 antibody (Santa Cruz, sc-5296) was used in western blot on human samples at 1:200 (fig 3). Nat Commun (2016) ncbi
mouse monoclonal (E-7)
  • immunoprecipitation; human; fig s4b
Santa Cruz Biotechnology Cdc20 antibody (santa cruz, sc-13162) was used in immunoprecipitation on human samples (fig s4b). Proc Natl Acad Sci U S A (2016) ncbi
mouse monoclonal (E-7)
  • other; human; loading ...; fig st1
In order to use size exclusion chromatography-microsphere-based affinity proteomics to study clinical samples obtained from pediatric acute leukemia patients, Santa Cruz Biotechnology Cdc20 antibody (SCBT, E-7) was used in other on human samples (fig st1). Mol Cell Proteomics (2016) ncbi
mouse monoclonal (E-7)
  • western blot; human; fig s1
Santa Cruz Biotechnology Cdc20 antibody (Santa Cruz, sc-13162) was used in western blot on human samples (fig s1). Proc Natl Acad Sci U S A (2016) ncbi
mouse monoclonal (H-7)
  • western blot; mouse; loading ...; fig 5a
In order to present the role of nuclear pore protein nup88 on anaphase-promoting complex, Santa Cruz Biotechnology Cdc20 antibody (Santa Cruz, sc-5296) was used in western blot on mouse samples (fig 5a). J Clin Invest (2016) ncbi
mouse monoclonal (E-7)
  • immunoprecipitation; human
  • western blot; human
In order to study the effect of MASTL on cyclin B1 degradation and its mechanism, Santa Cruz Biotechnology Cdc20 antibody (Santa Cruz, sc-13162) was used in immunoprecipitation on human samples and in western blot on human samples . Biol Open (2015) ncbi
mouse monoclonal (H-7)
  • western blot; human; 1:500
In order to study CDC26 phosphorylation and its effect on the tetratricopeptide repeat subcomplex of APC/C, Santa Cruz Biotechnology Cdc20 antibody (Santa Cruz Biotechnology, sc-5296) was used in western blot on human samples at 1:500. PLoS ONE (2015) ncbi
mouse monoclonal (E-7)
  • immunoprecipitation; human; 1:500
In order to explore how Nek2 isoform A affects anaphase-promoting complex/cyclosome containing Cdc20, Santa Cruz Biotechnology Cdc20 antibody (Santa Cruz Biotechnology, sc-13162) was used in immunoprecipitation on human samples at 1:500. J Cell Sci (2015) ncbi
mouse monoclonal (E-7)
  • immunocytochemistry; human; fig 2
  • western blot; human; 1:500; fig 3
Santa Cruz Biotechnology Cdc20 antibody (Santa Cruz, sc-13162) was used in immunocytochemistry on human samples (fig 2) and in western blot on human samples at 1:500 (fig 3). Nature (2015) ncbi
mouse monoclonal (E-7)
  • immunocytochemistry; human; fig 6a
Santa Cruz Biotechnology Cdc20 antibody (Santa, SC-13162) was used in immunocytochemistry on human samples (fig 6a). Proc Natl Acad Sci U S A (2014) ncbi
mouse monoclonal (E-7)
  • immunohistochemistry; human; 1:500
  • western blot; human
Santa Cruz Biotechnology Cdc20 antibody (Santa Cruz, SC-13162) was used in immunohistochemistry on human samples at 1:500 and in western blot on human samples . Oncotarget (2014) ncbi
mouse monoclonal (H-7)
  • western blot; human; fig 2b
In order to report that nuclear pore complexes in interphase cells function as scaffolds for anaphase-inhibitory signaling, Santa Cruz Biotechnology Cdc20 antibody (Santa, sc-5296) was used in western blot on human samples (fig 2b). Cell (2014) ncbi
mouse monoclonal (E-7)
  • immunohistochemistry; mouse; 1:100
  • western blot; mouse
In order to study the role of Cdk1 signaling in the mechanism by which filamin B modulates the proliferation and differentiation of chondrocytes, Santa Cruz Biotechnology Cdc20 antibody (Santa Cruz, sc-13162) was used in immunohistochemistry on mouse samples at 1:100 and in western blot on mouse samples . PLoS ONE (2014) ncbi
mouse monoclonal (E-7)
  • western blot; human; 1:1000
Santa Cruz Biotechnology Cdc20 antibody (Santa, sc-13162) was used in western blot on human samples at 1:1000. EMBO Rep (2014) ncbi
mouse monoclonal (E-7)
  • western blot; human
Santa Cruz Biotechnology Cdc20 antibody (Santa Cruz, sc-13162) was used in western blot on human samples . Mol Cell Biol (2013) ncbi
Cell Signaling Technology
rabbit polyclonal
  • western blot; mouse; fig 1
Cell Signaling Technology Cdc20 antibody (Cell Signal, 4823) was used in western blot on mouse samples (fig 1). Cell Rep (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 7
Cell Signaling Technology Cdc20 antibody (Cell Signaling, 4823) was used in western blot on human samples (fig 7). Oncotarget (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 6
In order to determine the role of Epstein-Barr virus protein kinase integration in mitotic signaling by phosphoproteomic profiling, Cell Signaling Technology Cdc20 antibody (Cell Signaling Technology, 4823) was used in western blot on human samples (fig 6). PLoS Pathog (2015) ncbi
rabbit polyclonal
  • western blot; human; loading ...; fig 6d
Cell Signaling Technology Cdc20 antibody (Cell Signalling, 8038) was used in western blot on human samples (fig 6d). Mol Cell Proteomics (2015) ncbi
rabbit polyclonal
  • western blot; mouse
Cell Signaling Technology Cdc20 antibody (Cell Signaling, 4823S) was used in western blot on mouse samples . Mol Cell Biol (2014) ncbi
Articles Reviewed
  1. Allan L, Skowyra A, Rogers K, Zeller D, Clarke P. Atypical APC/C-dependent degradation of Mcl-1 provides an apoptotic timer during mitotic arrest. EMBO J. 2018;37: pubmed publisher
  2. Zhang J, Bu X, Wang H, Zhu Y, Geng Y, Nihira N, et al. Cyclin D-CDK4 kinase destabilizes PD-L1 via cullin 3-SPOP to control cancer immune surveillance. Nature. 2018;553:91-95 pubmed publisher
  3. Hein J, Hertz E, Garvanska D, Kruse T, Nilsson J. Distinct kinetics of serine and threonine dephosphorylation are essential for mitosis. Nat Cell Biol. 2017;19:1433-1440 pubmed publisher
  4. Li J, Dang N, Wood D, Huang J. The kinetochore-dependent and -independent formation of the CDC20-MAD2 complex and its functions in HeLa cells. Sci Rep. 2017;7:41072 pubmed publisher
  5. Garvanska D, Larsen M, Nilsson J. Synergistic inhibition of the APC/C by the removal of APC15 in HCT116 cells lacking UBE2C. Biol Open. 2016;5:1441-1448 pubmed publisher
  6. Ho T, Guilbaud G, Blow J, Sale J, Watson C. The KRAB Zinc Finger Protein Roma/Zfp157 Is a Critical Regulator of Cell-Cycle Progression and Genomic Stability. Cell Rep. 2016;15:724-734 pubmed publisher
  7. Salsi V, Fantini S, Zappavigna V. NUP98 fusion oncoproteins interact with the APC/C(Cdc20) as a pseudosubstrate and prevent mitotic checkpoint complex binding. Cell Cycle. 2016;15:2275-87 pubmed publisher
  8. Sedgwick G, Larsen M, Lischetti T, Streicher W, Jersie Christensen R, Olsen J, et al. Conformation-specific anti-Mad2 monoclonal antibodies for the dissection of checkpoint signaling. MAbs. 2016;8:689-97 pubmed publisher
  9. Hein J, Nilsson J. Interphase APC/C-Cdc20 inhibition by cyclin A2-Cdk2 ensures efficient mitotic entry. Nat Commun. 2016;7:10975 pubmed publisher
  10. Jia L, Li B, Yu H. The Bub1-Plk1 kinase complex promotes spindle checkpoint signalling through Cdc20 phosphorylation. Nat Commun. 2016;7:10818 pubmed publisher
  11. Craney A, Kelly A, Jia L, Fedrigo I, Yu H, Rape M. Control of APC/C-dependent ubiquitin chain elongation by reversible phosphorylation. Proc Natl Acad Sci U S A. 2016;113:1540-5 pubmed publisher
  12. Kanderová V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, et al. High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016;15:1246-61 pubmed publisher
  13. Kaisari S, Sitry Shevah D, Miniowitz Shemtov S, Hershko A. Intermediates in the assembly of mitotic checkpoint complexes and their role in the regulation of the anaphase-promoting complex. Proc Natl Acad Sci U S A. 2016;113:966-71 pubmed publisher
  14. Naylor R, Jeganathan K, Cao X, van Deursen J. Nuclear pore protein NUP88 activates anaphase-promoting complex to promote aneuploidy. J Clin Invest. 2016;126:543-59 pubmed publisher
  15. Lub S, Maes A, Maes K, De Veirman K, De Bruyne E, Menu E, et al. Inhibiting the anaphase promoting complex/cyclosome induces a metaphase arrest and cell death in multiple myeloma cells. Oncotarget. 2016;7:4062-76 pubmed publisher
  16. Li R, Liao G, Nirujogi R, Pinto S, Shaw P, Huang T, et al. Phosphoproteomic Profiling Reveals Epstein-Barr Virus Protein Kinase Integration of DNA Damage Response and Mitotic Signaling. PLoS Pathog. 2015;11:e1005346 pubmed publisher
  17. McCloy R, Parker B, Rogers S, Chaudhuri R, Gayevskiy V, Hoffman N, et al. Global Phosphoproteomic Mapping of Early Mitotic Exit in Human Cells Identifies Novel Substrate Dephosphorylation Motifs. Mol Cell Proteomics. 2015;14:2194-212 pubmed publisher
  18. Voets E, Wolthuis R. MASTL promotes cyclin B1 destruction by enforcing Cdc20-independent binding of cyclin B1 to the APC/C. Biol Open. 2015;4:484-95 pubmed publisher
  19. Masuda K, Chiyoda T, Sugiyama N, Segura Cabrera A, Kabe Y, Ueki A, et al. LATS1 and LATS2 phosphorylate CDC26 to modulate assembly of the tetratricopeptide repeat subcomplex of APC/C. PLoS ONE. 2015;10:e0118662 pubmed publisher
  20. Boekhout M, Wolthuis R. Nek2A destruction marks APC/C activation at the prophase-to-prometaphase transition by spindle-checkpoint-restricted Cdc20. J Cell Sci. 2015;128:1639-53 pubmed publisher
  21. Izawa D, Pines J. The mitotic checkpoint complex binds a second CDC20 to inhibit active APC/C. Nature. 2015;517:631-4 pubmed publisher
  22. Perry M, Dufour C, Eichner L, Tsang D, Deblois G, Muller W, et al. ERBB2 deficiency alters an E2F-1-dependent adaptive stress response and leads to cardiac dysfunction. Mol Cell Biol. 2014;34:4232-43 pubmed publisher
  23. Han J, Vitre B, Fachinetti D, Cleveland D. Bimodal activation of BubR1 by Bub3 sustains mitotic checkpoint signaling. Proc Natl Acad Sci U S A. 2014;111:E4185-93 pubmed publisher
  24. Hu K, Liao D, Wu W, Han A, Shi H, Wang F, et al. Targeting the anaphase-promoting complex/cyclosome (APC/C)- bromodomain containing 7 (BRD7) pathway for human osteosarcoma. Oncotarget. 2014;5:3088-100 pubmed
  25. Rodriguez Bravo V, Maciejowski J, Corona J, Buch H, Collin P, Kanemaki M, et al. Nuclear pores protect genome integrity by assembling a premitotic and Mad1-dependent anaphase inhibitor. Cell. 2014;156:1017-31 pubmed publisher
  26. Hu J, Lu J, Lian G, Zhang J, Hecht J, Sheen V. Filamin B regulates chondrocyte proliferation and differentiation through Cdk1 signaling. PLoS ONE. 2014;9:e89352 pubmed publisher
  27. Hein J, Nilsson J. Stable MCC binding to the APC/C is required for a functional spindle assembly checkpoint. EMBO Rep. 2014;15:264-72 pubmed publisher
  28. Lim H, Dimova N, Tan M, Sigoillot F, King R, Shi Y. The G2/M regulator histone demethylase PHF8 is targeted for degradation by the anaphase-promoting complex containing CDC20. Mol Cell Biol. 2013;33:4166-80 pubmed publisher