This is a Validated Antibody Database (VAD) review about rat Ccl12, based on 9 published articles (read how Labome selects the articles), using Ccl12 antibody in all methods. It is aimed to help Labome visitors find the most suited Ccl12 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Ccl12 synonym: MCP-5

Novus Biologicals
mouse monoclonal (2D8)
  • immunohistochemistry - paraffin section; human; 1:100; loading ...; fig e10g
Novus Biologicals Ccl12 antibody (Novus, NBP2- 2211555) was used in immunohistochemistry - paraffin section on human samples at 1:100 (fig e10g). Nature (2019) ncbi
mouse monoclonal (2D8)
  • immunohistochemistry - paraffin section; human; 1:4000; loading ...; fig 3f
  • immunohistochemistry - paraffin section; mouse; 1:500; loading ...; fig 2d
Novus Biologicals Ccl12 antibody (Novus Biologicals, NBP-22115) was used in immunohistochemistry - paraffin section on human samples at 1:4000 (fig 3f) and in immunohistochemistry - paraffin section on mouse samples at 1:500 (fig 2d). Nat Commun (2019) ncbi
mouse monoclonal (2D8)
  • immunohistochemistry; mouse; loading ...; fig 3b
Novus Biologicals Ccl12 antibody (Novus Biologicals, 2D8) was used in immunohistochemistry on mouse samples (fig 3b). Diabetes (2017) ncbi
mouse monoclonal (2D8)
  • western blot; mouse; fig 4
Novus Biologicals Ccl12 antibody (Novus, NBP2-22115S) was used in western blot on mouse samples (fig 4). Proc Natl Acad Sci U S A (2016) ncbi
mouse monoclonal (2D8)
  • immunohistochemistry - paraffin section; human; 1:100
In order to investigate the relationship between macrophages and T-box transcription factor Brachyury in tumor progression and metastasis, Novus Biologicals Ccl12 antibody (Novus Biologicals, NBP2-22115) was used in immunohistochemistry - paraffin section on human samples at 1:100. Tumour Biol (2015) ncbi
BioLegend
hamsters monoclonal (2H5)
  • blocking or activating experiments; mouse; ; fig 3g
  • flow cytometry; mouse; loading ...; fig 4a
BioLegend Ccl12 antibody (BioLegend, 2H5) was used in blocking or activating experiments on mouse samples at (fig 3g) and in flow cytometry on mouse samples (fig 4a). Sci Adv (2020) ncbi
hamsters monoclonal (2H5)
  • immunohistochemistry - paraffin section; mouse
BioLegend Ccl12 antibody (Biolegend, 505902) was used in immunohistochemistry - paraffin section on mouse samples . Cardiovasc Res (2015) ncbi
hamsters monoclonal (2H5)
  • dot blot; human; tbl s1
In order to use polydimethylsiloxane microchambers to co-detect 42 immune effector proteins secreted from a single LPS-stimulation macrophage, BioLegend Ccl12 antibody (Biolegend, 505902) was used in dot blot on human samples (tbl s1). Proc Natl Acad Sci U S A (2015) ncbi
Invitrogen
hamsters monoclonal (2H5)
  • ELISA; mouse; fig 2b
  • ELISA; human; fig 1b
In order to optimize innate defense regulator derivatives with enhanced chemokine induction, Invitrogen Ccl12 antibody (eBioscience, 2H5) was used in ELISA on mouse samples (fig 2b) and in ELISA on human samples (fig 1b). J Immunol (2010) ncbi
Articles Reviewed
  1. Fu Y, Ding Y, Wang Q, Zhu F, Tan Y, Lu X, et al. Blood-stage malaria parasites manipulate host innate immune responses through the induction of sFGL2. Sci Adv. 2020;6:eaay9269 pubmed publisher
  2. Ramachandran P, Dobie R, Wilson Kanamori J, Dora E, Henderson B, Luu N, et al. Resolving the fibrotic niche of human liver cirrhosis at single-cell level. Nature. 2019;575:512-518 pubmed publisher
  3. Pascual García M, Bonfill Teixidor E, Planas Rigol E, Rubio Perez C, Iurlaro R, Arias A, et al. LIF regulates CXCL9 in tumor-associated macrophages and prevents CD8+ T cell tumor-infiltration impairing anti-PD1 therapy. Nat Commun. 2019;10:2416 pubmed publisher
  4. Portillo J, Lopez Corcino Y, Miao Y, Tang J, Sheibani N, Kern T, et al. CD40 in Retinal Müller Cells Induces P2X7-Dependent Cytokine Expression in Macrophages/Microglia in Diabetic Mice and Development of Early Experimental Diabetic Retinopathy. Diabetes. 2017;66:483-493 pubmed publisher
  5. Nishio M, Sugimachi K, Goto H, Wang J, Morikawa T, Miyachi Y, et al. Dysregulated YAP1/TAZ and TGF-β signaling mediate hepatocarcinogenesis in Mob1a/1b-deficient mice. Proc Natl Acad Sci U S A. 2016;113:E71-80 pubmed publisher
  6. Wan W, Liu Q, Lionakis M, Marino A, Anderson S, Swamydas M, et al. Atypical chemokine receptor 1 deficiency reduces atherogenesis in ApoE-knockout mice. Cardiovasc Res. 2015;106:478-87 pubmed publisher
  7. Chen S, Jiao J, Jiang D, Wan Z, Li L, Li K, et al. T-box transcription factor Brachyury in lung cancer cells inhibits macrophage infiltration by suppressing CCL2 and CCL4 chemokines. Tumour Biol. 2015;36:5881-90 pubmed publisher
  8. Lu Y, Xue Q, Eisele M, Sulistijo E, Brower K, Han L, et al. Highly multiplexed profiling of single-cell effector functions reveals deep functional heterogeneity in response to pathogenic ligands. Proc Natl Acad Sci U S A. 2015;112:E607-15 pubmed publisher
  9. Nijnik A, Madera L, Ma S, Waldbrook M, Elliott M, Easton D, et al. Synthetic cationic peptide IDR-1002 provides protection against bacterial infections through chemokine induction and enhanced leukocyte recruitment. J Immunol. 2010;184:2539-50 pubmed publisher