This is a Validated Antibody Database (VAD) review about rat Capn1, based on 25 published articles (read how Labome selects the articles), using Capn1 antibody in all methods. It is aimed to help Labome visitors find the most suited Capn1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Capn1 synonym: calpain-1 catalytic subunit; CANP 1; calcium-activated neutral proteinase 1; calpain 1, (mu/I) large subunit; calpain mu-type; calpain-1 large subunit; micromolar-calpain; muCANP

Knockout validation
Cell Signaling Technology
rabbit polyclonal
  • western blot knockout validation; mouse; 1:1000; loading ...; fig 4e
Cell Signaling Technology Capn1 antibody (Cell Signaling, 2556) was used in western blot knockout validation on mouse samples at 1:1000 (fig 4e). Cell Rep (2016) ncbi
Invitrogen
mouse monoclonal (107-82)
  • western blot; cow; 1:1000; loading ...; tbl 1
In order to examine the relationships among a list of 23 protein biomarkers with CIE-L*a*b* meat color traits and ultimate pH on muscles of Maine-Anjou cows, Invitrogen Capn1 antibody (ABR, MA3-942) was used in western blot on cow samples at 1:1000 (tbl 1). J Agric Food Chem (2017) ncbi
mouse monoclonal (15C10)
  • western blot; human; 1:1000; loading ...; fig 3a
In order to determine the role of calcium regulation in sporadic inclusion body myositis, Invitrogen Capn1 antibody (Thermo Fischer, MA1-12434) was used in western blot on human samples at 1:1000 (fig 3a). Acta Neuropathol Commun (2017) ncbi
mouse monoclonal (9A4H8D3)
  • western blot; sheep; 1:1000; loading ...; fig 3
In order to report the effects of phosphorylation on the activity of calpain and its sensitivity to temperature and calcium ions, Invitrogen Capn1 antibody (Thermo Scientific, MA3-940) was used in western blot on sheep samples at 1:1000 (fig 3). Food Chem (2017) ncbi
mouse monoclonal (9A4H8D3)
  • western blot; sheep; 1:5000
In order to study the effects of tannins on meat quality, Invitrogen Capn1 antibody (Fisher Scientific, MA3-940) was used in western blot on sheep samples at 1:5000. J Sci Food Agric (2016) ncbi
mouse monoclonal (9A4H8D3)
  • western blot; pig
Invitrogen Capn1 antibody (Thermo Scientific, 9A4H8D3) was used in western blot on pig samples . Int J Legal Med (2016) ncbi
mouse monoclonal (9A4H8D3)
  • western blot; pig; 1:10000
In order to investigate the differences in calpain system, desmin degradation, pH values and water holding capacity between muscles of commercial Meishan and Duroc x Landrace x Yorkshire crossbred pigs, Invitrogen Capn1 antibody (Affinity Bioreagents, MA3-940) was used in western blot on pig samples at 1:10000. Anim Sci J (2016) ncbi
mouse monoclonal (9A4H8D3)
  • western blot; cow; 1:5000
In order to determine the effect of calcium lactate/phosphate enhancement on the quality of beef round cuts in high-oxygen modified atmosphere, Invitrogen Capn1 antibody (Thermo, MA3-940) was used in western blot on cow samples at 1:5000. Meat Sci (2015) ncbi
mouse monoclonal (9A4H8D3)
  • western blot; pig; 1:10000
In order to investigate the differences in protein modifications between pale, soft, and exudative and red, firm, and nonexudative pork during postmortem aging, Invitrogen Capn1 antibody (Affinity Bioreagents, MA3-940) was used in western blot on pig samples at 1:10000. J Anim Sci (2014) ncbi
mouse monoclonal (9A4H8D3)
  • western blot; pig; 1:10000
In order to investigate the effect of nitric oxide (NO) on calpain activation, protein proteolysis, and oxidation in porcine muscles, Invitrogen Capn1 antibody (Aynity Bioreagents, MA3-940) was used in western blot on pig samples at 1:10000. J Agric Food Chem (2014) ncbi
mouse monoclonal (9A4H8D3)
  • immunocytochemistry; rat
  • western blot; rat
In order to study the distinct roles of different calpain isoforms in synaptic neuroprotection and extrasynaptic neurodegeneration mediated by the NMDA receptor, Invitrogen Capn1 antibody (Thermo Scientific, MA3-940) was used in immunocytochemistry on rat samples and in western blot on rat samples . J Neurosci (2013) ncbi
Enzo Life Sciences
mouse monoclonal (9A4H8D3)
  • western blot; cow; 1:1000; loading ...; tbl 1
In order to examine the relationships among a list of 23 protein biomarkers with CIE-L*a*b* meat color traits and ultimate pH on muscles of Maine-Anjou cows, Enzo Life Sciences Capn1 antibody (Alexis, 9A4H8D3) was used in western blot on cow samples at 1:1000 (tbl 1). J Agric Food Chem (2017) ncbi
mouse monoclonal (9A4H8D3)
  • western blot; mouse; 1:1000; fig 4
Enzo Life Sciences Capn1 antibody (Enzo, ALX-804-050-R100) was used in western blot on mouse samples at 1:1000 (fig 4). Nat Commun (2015) ncbi
mouse monoclonal (9A4H8D3)
  • western blot; cow; 1:1000
Enzo Life Sciences Capn1 antibody (Alexis, 9A4H8D3) was used in western blot on cow samples at 1:1000. J Agric Food Chem (2014) ncbi
Abcam
rabbit polyclonal
  • western blot; human; fig 2
Abcam Capn1 antibody (Abcam, 28258) was used in western blot on human samples (fig 2). J Musculoskelet Neuronal Interact (2014) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; mouse; loading ...; fig 8b
  • immunohistochemistry; mouse; loading ...; fig 1c
  • western blot; mouse; loading ...; fig 1a
Abcam Capn1 antibody (Abcam, ab39170) was used in chromatin immunoprecipitation on mouse samples (fig 8b), in immunohistochemistry on mouse samples (fig 1c) and in western blot on mouse samples (fig 1a). Biochem J (2014) ncbi
rabbit monoclonal (EPR3319)
  • immunohistochemistry - paraffin section; rat
In order to identify the mechanism of colistin-induced nephropathy, Abcam Capn1 antibody (Abcam, ab108400) was used in immunohistochemistry - paraffin section on rat samples . Antimicrob Agents Chemother (2013) ncbi
Santa Cruz Biotechnology
mouse monoclonal (D-11)
  • western blot; mouse; 1:2000; fig 3
Santa Cruz Biotechnology Capn1 antibody (Santa Cruz, sc-271313) was used in western blot on mouse samples at 1:2000 (fig 3). ASN Neuro (2013) ncbi
Cell Signaling Technology
rabbit polyclonal
  • western blot; human; 1:1000; fig 5
Cell Signaling Technology Capn1 antibody (CST, 2556) was used in western blot on human samples at 1:1000 (fig 5). Cell Death Dis (2016) ncbi
rabbit polyclonal
  • western blot knockout validation; mouse; 1:1000; loading ...; fig 4e
Cell Signaling Technology Capn1 antibody (Cell Signaling, 2556) was used in western blot knockout validation on mouse samples at 1:1000 (fig 4e). Cell Rep (2016) ncbi
rabbit polyclonal
  • western blot; rat; 1:500; fig 3
Cell Signaling Technology Capn1 antibody (Cell Signaling, 2556) was used in western blot on rat samples at 1:500 (fig 3). PLoS ONE (2016) ncbi
rabbit polyclonal
  • western blot; rat; 1:1000; fig 4
Cell Signaling Technology Capn1 antibody (Cell signaling, 2556S) was used in western blot on rat samples at 1:1000 (fig 4). Cell Death Dis (2016) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000; fig 3
Cell Signaling Technology Capn1 antibody (Cell Signaling, 2556) was used in western blot on mouse samples at 1:1000 (fig 3). Mol Neurodegener (2015) ncbi
rabbit polyclonal
  • western blot; rat
Cell Signaling Technology Capn1 antibody (CST, 2556) was used in western blot on rat samples . FASEB J (2014) ncbi
Sigma-Aldrich
mouse monoclonal (15C10)
  • western blot; human; 1:1000; fig 10
Sigma-Aldrich Capn1 antibody (Sigma-Aldrich, C0355) was used in western blot on human samples at 1:1000 (fig 10). J Am Heart Assoc (2016) ncbi
mouse monoclonal (15C10)
  • western blot; human; fig 4
Sigma-Aldrich Capn1 antibody (Sigma, C5736) was used in western blot on human samples (fig 4). Cell Biosci (2015) ncbi
mouse monoclonal (15C10)
  • western blot; mouse; loading ...; fig 2
Sigma-Aldrich Capn1 antibody (Sigma, C5736) was used in western blot on mouse samples (fig 2). PLoS ONE (2015) ncbi
Articles Reviewed
  1. Gagaoua M, Couvreur S, Le Bec G, Aminot G, Picard B. Associations among Protein Biomarkers and pH and Color Traits in Longissimus thoracis and Rectus abdominis Muscles in Protected Designation of Origin Maine-Anjou Cull Cows. J Agric Food Chem. 2017;65:3569-3580 pubmed publisher
  2. Amici D, Pinal Fernández I, Mázala D, Lloyd T, Corse A, Christopher Stine L, et al. Calcium dysregulation, functional calpainopathy, and endoplasmic reticulum stress in sporadic inclusion body myositis. Acta Neuropathol Commun. 2017;5:24 pubmed publisher
  3. Du M, Li X, Li Z, Li M, Gao L, Zhang D. Phosphorylation inhibits the activity of ?-calpain at different incubation temperatures and Ca2+ concentrations in vitro. Food Chem. 2017;228:649-655 pubmed publisher
  4. Hammers D, Sleeper M, Forbes S, Shima A, Walter G, Sweeney H. Tadalafil Treatment Delays the Onset of Cardiomyopathy in Dystrophin-Deficient Hearts. J Am Heart Assoc. 2016;5: pubmed publisher
  5. Anta B, Pérez Rodríguez A, Castro J, García Domínguez C, Ibiza S, Martínez N, et al. PGA1-induced apoptosis involves specific activation of H-Ras and N-Ras in cellular endomembranes. Cell Death Dis. 2016;7:e2311 pubmed publisher
  6. Wang Y, Hersheson J, López D, Hammer M, Liu Y, Lee K, et al. Defects in the CAPN1 Gene Result in Alterations in Cerebellar Development and Cerebellar Ataxia in Mice and Humans. Cell Rep. 2016;16:79-91 pubmed publisher
  7. Talbert E, Smuder A, Kwon O, Sollanek K, Wiggs M, Powers S. Blockage of the Ryanodine Receptor via Azumolene Does Not Prevent Mechanical Ventilation-Induced Diaphragm Atrophy. PLoS ONE. 2016;11:e0148161 pubmed publisher
  8. Shinde V, Kotla P, Strang C, Gorbatyuk M. Unfolded protein response-induced dysregulation of calcium homeostasis promotes retinal degeneration in rat models of autosomal dominant retinitis pigmentosa. Cell Death Dis. 2016;7:e2085 pubmed publisher
  9. Choudhury S, Liu Y, Clark A, Pang I. Caspase-7: a critical mediator of optic nerve injury-induced retinal ganglion cell death. Mol Neurodegener. 2015;10:40 pubmed publisher
  10. Cui W, Zhou J, Dehne N, Brüne B. Hypoxia induces calpain activity and degrades SMAD2 to attenuate TGFβ signaling in macrophages. Cell Biosci. 2015;5:36 pubmed publisher
  11. Giordano C, Lemaire C, Li T, Kimoff R, Petrof B. Autophagy-associated atrophy and metabolic remodeling of the mouse diaphragm after short-term intermittent hypoxia. PLoS ONE. 2015;10:e0131068 pubmed publisher
  12. Girard M, Dohme Meier F, Silacci P, Ampuero Kragten S, Kreuzer M, Bee G. Forage legumes rich in condensed tannins may increase n-3 fatty acid levels and sensory quality of lamb meat. J Sci Food Agric. 2016;96:1923-33 pubmed publisher
  13. Foditsch E, Saenger A, Monticelli F. Skeletal muscle proteins: a new approach to delimitate the time since death. Int J Legal Med. 2016;130:433-40 pubmed publisher
  14. Wang J, Yan X, Liu R, Fu Q, Zhou G, Zhang W. Differences in calpain system, desmin degradation and water holding capacity between commercial Meishan and Duroc × Landrace × Yorkshire crossbred pork. Anim Sci J. 2016;87:109-16 pubmed publisher
  15. Sato S, Kawamata Y, Takahashi A, Imai Y, Hanyu A, Okuma A, et al. Ablation of the p16(INK4a) tumour suppressor reverses ageing phenotypes of klotho mice. Nat Commun. 2015;6:7035 pubmed publisher
  16. Blottner D, Bosutti A, Degens H, Schiffl G, Gutsmann M, Buehlmeier J, et al. Whey protein plus bicarbonate supplement has little effects on structural atrophy and proteolysis marker immunopatterns in skeletal muscle disuse during 21 days of bed rest. J Musculoskelet Neuronal Interact. 2014;14:432-44 pubmed
  17. Cruzen S, Kim Y, Lonergan S, Grubbs J, Fritchen A, Huff Lonergan E. Effect of early postmortem enhancement of calcium lactate/phosphate on quality attributes of beef round muscles under different packaging systems. Meat Sci. 2015;101:63-72 pubmed publisher
  18. Picard B, Gagaoua M, Micol D, Cassar Malek I, Hocquette J, Terlouw C. Inverse relationships between biomarkers and beef tenderness according to contractile and metabolic properties of the muscle. J Agric Food Chem. 2014;62:9808-18 pubmed publisher
  19. Yin Y, Zhang W, Zhou G, Guo B. Comparison of protein degradation, protein oxidation, and ?-calpain activation between pale, soft, and exudative and red, firm, and nonexudative pork during postmortem aging. J Anim Sci. 2014;92:3745-52 pubmed publisher
  20. Li Y, Liu R, Zhang W, Fu Q, Liu N, Zhou G. Effect of nitric oxide on ?-calpain activation, protein proteolysis, and protein oxidation of pork during post-mortem aging. J Agric Food Chem. 2014;62:5972-7 pubmed publisher
  21. Smith I, Godinez G, Singh B, McCaughey K, Alcantara R, Gururaja T, et al. Inhibition of Janus kinase signaling during controlled mechanical ventilation prevents ventilation-induced diaphragm dysfunction. FASEB J. 2014;28:2790-803 pubmed publisher
  22. Arnandis T, Ferrer Vicens I, Torres L, García C, García Trevijano E, Zaragoza R, et al. Differential functions of calpain 1 during epithelial cell death and adipocyte differentiation in mammary gland involution. Biochem J. 2014;459:355-68 pubmed publisher
  23. Wang Y, Briz V, Chishti A, Bi X, Baudry M. Distinct roles for ?-calpain and m-calpain in synaptic NMDAR-mediated neuroprotection and extrasynaptic NMDAR-mediated neurodegeneration. J Neurosci. 2013;33:18880-92 pubmed publisher
  24. Li H, Zhang N, Sun G, Ding S. Inhibition of the group I mGluRs reduces acute brain damage and improves long-term histological outcomes after photothrombosis-induced ischaemia. ASN Neuro. 2013;5:195-207 pubmed publisher
  25. Ozkan G, Ulusoy S, Orem A, Alkanat M, Mungan S, Yulug E, et al. How does colistin-induced nephropathy develop and can it be treated?. Antimicrob Agents Chemother. 2013;57:3463-9 pubmed publisher