This is a Validated Antibody Database (VAD) review about rat Calm1, based on 17 published articles (read how Labome selects the articles), using Calm1 antibody in all methods. It is aimed to help Labome visitors find the most suited Calm1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Calm1 synonym: CaMI; Calm; Cam1; calmodulin-1; Calmodulin 1 (phosphorylase kinase, delta); caM; calmodulin I

Abcam
mouse monoclonal (2D1)
  • ELISA; human
Abcam Calm1 antibody (Abcam, ab2860) was used in ELISA on human samples . Eur J Neurosci (2017) ncbi
rabbit monoclonal (EP799Y)
  • western blot; pig; 1:1000; loading ...; fig 8b
In order to use CRISPR/Cas9 to generate pigs with a deletion of Exon 7 of the CD163 gene, encoding the scavenger receptor cysteine-rich domain 5, Abcam Calm1 antibody (Abcam, ab45689) was used in western blot on pig samples at 1:1000 (fig 8b). PLoS Pathog (2017) ncbi
rabbit monoclonal (EP799Y)
  • western blot; mouse; loading ...; fig 3c
Abcam Calm1 antibody (Abcam, EP799Y) was used in western blot on mouse samples (fig 3c). Mol Cell Proteomics (2016) ncbi
rabbit monoclonal (EP799Y)
  • immunocytochemistry; rat; 1:200
Abcam Calm1 antibody (Abcam, ab45689) was used in immunocytochemistry on rat samples at 1:200. J Cell Biochem (2015) ncbi
rabbit monoclonal (EP799Y)
  • immunocytochemistry; mouse; loading ...; fig 4d
  • western blot; mouse; loading ...; fig 3a
Abcam Calm1 antibody (abcam, ab45689) was used in immunocytochemistry on mouse samples (fig 4d) and in western blot on mouse samples (fig 3a). PLoS ONE (2015) ncbi
rabbit monoclonal (EP799Y)
  • immunohistochemistry - frozen section; mouse; 1:500
  • western blot; mouse
Abcam Calm1 antibody (Abcam, ab45689) was used in immunohistochemistry - frozen section on mouse samples at 1:500 and in western blot on mouse samples . J Proteomics (2014) ncbi
mouse monoclonal (2D1)
  • immunohistochemistry - paraffin section; mouse; 1:100
Abcam Calm1 antibody (Abcam, ab2860) was used in immunohistochemistry - paraffin section on mouse samples at 1:100. PLoS ONE (2014) ncbi
rabbit monoclonal (EP799Y)
  • western blot; common platanna; 1:5000
Abcam Calm1 antibody (Abcam, ab45689) was used in western blot on common platanna samples at 1:5000. J Gen Physiol (2013) ncbi
rabbit monoclonal (EP799Y)
  • western blot; human; 1:3000
  • western blot; rat; 1:3000
Abcam Calm1 antibody (Abcam, ab45689) was used in western blot on human samples at 1:3000 and in western blot on rat samples at 1:3000. Neurochem Int (2013) ncbi
rabbit monoclonal (EP799Y)
  • western blot; human; 1:1000
Abcam Calm1 antibody (Abcam, ab45689) was used in western blot on human samples at 1:1000. BMC Cancer (2012) ncbi
Invitrogen
mouse monoclonal (2D1)
  • western blot; mouse; 1:1000; fig 5a
In order to examine the effects of sildenafil on skeletal muscle, Invitrogen Calm1 antibody (Thermo Scientific, MA3-917) was used in western blot on mouse samples at 1:1000 (fig 5a). Exp Mol Med (2016) ncbi
mouse monoclonal (6D4)
  • western blot; rat
In order to examine calcium-related proteins in the intrinsic laryngeal muscles, cricothyroid, and tibialis anterior muscles from male Sprague-Dawley rats, Invitrogen Calm1 antibody (Affinity Bioreagents, MA3-918) was used in western blot on rat samples . Physiol Rep (2015) ncbi
mouse monoclonal (2D1)
  • blocking or activating experiments; rat; 5 ug/ml
In order to investigate anit-Yo antibodies in Purkinje cell death, Invitrogen Calm1 antibody (Pierce Antibodies, MA3-917) was used in blocking or activating experiments on rat samples at 5 ug/ml. PLoS ONE (2015) ncbi
mouse monoclonal (2D1)
  • immunohistochemistry - free floating section; shrews; 1:100
In order to study the mechanism for serotonin 5-HT3 receptor-mediated vomiting, Invitrogen Calm1 antibody (Thermo, MA3-917) was used in immunohistochemistry - free floating section on shrews samples at 1:100. PLoS ONE (2014) ncbi
mouse monoclonal (2D1)
  • western blot; fruit fly; 1:500; fig s4
In order to analyze Drosophila pericentrin and its requirement for interaction with calmodulin for function at centrosomes and neuronal basal bodies but not sperm basal bodies, Invitrogen Calm1 antibody (Thermo Scientific, MA3-917) was used in western blot on fruit fly samples at 1:500 (fig s4). Mol Biol Cell (2014) ncbi
Cell Signaling Technology
rabbit polyclonal
  • western blot; mouse; fig 7
In order to test if sigma1R is involved in the negative control that glutamate N-methyl-d-aspartate acid receptors exert on opioid antinociception, Cell Signaling Technology Calm1 antibody (Cell Signaling, 4830) was used in western blot on mouse samples (fig 7). Antioxid Redox Signal (2015) ncbi
rabbit polyclonal
  • immunocytochemistry; rat; 1:200
  • western blot; rat; 1:200
In order to identify the molecular mechanisms that regulate the enrichment of KCNQ channels at the neuronal axon, Cell Signaling Technology Calm1 antibody (Cell Signaling, 4830) was used in immunocytochemistry on rat samples at 1:200 and in western blot on rat samples at 1:200. PLoS ONE (2014) ncbi
Articles Reviewed
  1. Salman M, Kitchen P, Woodroofe M, Brown J, Bill R, Conner A, et al. Hypothermia increases aquaporin 4 (AQP4) plasma membrane abundance in human primary cortical astrocytes via a calcium/transient receptor potential vanilloid 4 (TRPV4)- and calmodulin-mediated mechanism. Eur J Neurosci. 2017;46:2542-2547 pubmed publisher
  2. Burkard C, Lillico S, Reid E, Jackson B, Mileham A, Ait Ali T, et al. Precision engineering for PRRSV resistance in pigs: Macrophages from genome edited pigs lacking CD163 SRCR5 domain are fully resistant to both PRRSV genotypes while maintaining biological function. PLoS Pathog. 2017;13:e1006206 pubmed publisher
  3. Huang M, Lee K, Kim K, Ahn M, Cho C, Kim D, et al. The maintenance ability and Ca2+ availability of skeletal muscle are enhanced by sildenafil. Exp Mol Med. 2016;48:e278 pubmed publisher
  4. Pehar M, Ball L, Sharma D, Harlan B, Comte Walters S, Neely B, et al. Changes in Protein Expression and Lysine Acetylation Induced by Decreased Glutathione Levels in Astrocytes. Mol Cell Proteomics. 2016;15:493-505 pubmed publisher
  5. Ferretti R, Marques M, Khurana T, Santo Neto H. Expression of calcium-buffering proteins in rat intrinsic laryngeal muscles. Physiol Rep. 2015;3: pubmed publisher
  6. Ulke Lemée A, Turner S, MacDonald J. In situ analysis of smoothelin-like 1 and calmodulin interactions in smooth muscle cells by proximity ligation. J Cell Biochem. 2015;116:2667-75 pubmed publisher
  7. Greenlee J, Clawson S, Hill K, Wood B, Clardy S, Tsunoda I, et al. Anti-Yo antibody uptake and interaction with its intracellular target antigen causes Purkinje cell death in rat cerebellar slice cultures: a possible mechanism for paraneoplastic cerebellar degeneration in humans with gynecological or breast cancers. PLoS ONE. 2015;10:e0123446 pubmed publisher
  8. Chung S, Gillies M, Sugiyama Y, Zhu L, Lee S, Shen W. Profiling of microRNAs involved in retinal degeneration caused by selective Müller cell ablation. PLoS ONE. 2015;10:e0118949 pubmed publisher
  9. Rodríguez Muñoz M, Sánchez Blázquez P, Herrero Labrador R, Martínez Murillo R, Merlos M, Vela J, et al. The σ1 receptor engages the redox-regulated HINT1 protein to bring opioid analgesia under NMDA receptor negative control. Antioxid Redox Signal. 2015;22:799-818 pubmed publisher
  10. Zhong W, Hutchinson T, Chebolu S, Darmani N. Serotonin 5-HT3 receptor-mediated vomiting occurs via the activation of Ca2+/CaMKII-dependent ERK1/2 signaling in the least shrew (Cryptotis parva). PLoS ONE. 2014;9:e104718 pubmed publisher
  11. Cavaretta J, Sherer K, Lee K, Kim E, Issema R, Chung H. Polarized axonal surface expression of neuronal KCNQ potassium channels is regulated by calmodulin interaction with KCNQ2 subunit. PLoS ONE. 2014;9:e103655 pubmed publisher
  12. Galletta B, Guillen R, Fagerstrom C, Brownlee C, Lerit D, Megraw T, et al. Drosophila pericentrin requires interaction with calmodulin for its function at centrosomes and neuronal basal bodies but not at sperm basal bodies. Mol Biol Cell. 2014;25:2682-94 pubmed publisher
  13. Zeng H, Rao X, Zhang L, Zhao X, Zhang W, Wang J, et al. Quantitative proteomics reveals olfactory input-dependent alterations in the mouse olfactory bulb proteome. J Proteomics. 2014;109:125-42 pubmed publisher
  14. Li R, Tan J, Chen L, Feng J, Liang W, Guo X, et al. Iqcg is essential for sperm flagellum formation in mice. PLoS ONE. 2014;9:e98053 pubmed publisher
  15. Morales P, Garneau L, Klein H, Lavoie M, Parent L, Sauve R. Contribution of the KCa3.1 channel-calmodulin interactions to the regulation of the KCa3.1 gating process. J Gen Physiol. 2013;142:37-60 pubmed publisher
  16. Yang W, Wang X, Duan C, Lu L, Yang H. Alpha-synuclein overexpression increases phospho-protein phosphatase 2A levels via formation of calmodulin/Src complex. Neurochem Int. 2013;63:180-94 pubmed publisher
  17. Makinoshima H, Ishii G, Kojima M, Fujii S, Higuchi Y, Kuwata T, et al. PTPRZ1 regulates calmodulin phosphorylation and tumor progression in small-cell lung carcinoma. BMC Cancer. 2012;12:537 pubmed publisher